Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,555,324 A→G 16.4% S116P (TCT→CCT)  maeA ← malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,555,3240AG16.4% 221.7 / 31.7 97S116P (TCT→CCT) maeAmalate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme
Reads supporting (aligned to +/- strand):  ref base A (33/47);  new base G (7/9);  total (41/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGT  >  NC_000913/1555225‑1555423
                                                                                                   |                                                                                                   
cTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGa                                                                                                     >  2:1689964/1‑100 (MQ=255)
cTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGa                                                                                                     >  2:2991807/1‑100 (MQ=255)
     atatTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAAc                                                                                                <  1:508540/100‑1 (MQ=255)
       attatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGc                                                                                              >  2:1811960/1‑100 (MQ=255)
       attatGGTTCGGCACGTTTTGCAGAATATCGTCCACATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGc                                                                                              <  2:4104094/100‑1 (MQ=255)
        ttatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCt                                                                                             >  1:2720176/1‑100 (MQ=255)
        ttatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCt                                                                                             >  1:1981618/1‑100 (MQ=255)
        ttatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCt                                                                                             >  1:4095063/1‑100 (MQ=255)
         tatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTc                                                                                            <  2:908140/100‑1 (MQ=255)
         tatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTc                                                                                            >  2:425009/1‑100 (MQ=255)
         tatGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTc                                                                                            <  2:1634993/100‑1 (MQ=255)
          atGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTca                                                                                           <  1:362484/100‑1 (MQ=255)
          atGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTca                                                                                           >  2:2933995/1‑100 (MQ=255)
           tGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTcac                                                                                          >  2:2197572/1‑100 (MQ=255)
            ggTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTcaca                                                                                         >  2:1414308/1‑100 (MQ=255)
            ggTTCGGCACGTTTTGCAGAATACCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTcaca                                                                                         >  2:2043911/1‑100 (MQ=255)
             gTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAg                                                                                        >  2:269703/1‑100 (MQ=255)
             gTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAg                                                                                        >  1:1069613/1‑100 (MQ=255)
             gTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAg                                                                                        <  2:2720160/100‑1 (MQ=255)
             gTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAg                                                                                        <  1:266168/100‑1 (MQ=255)
             gTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAg                                                                                        <  1:264925/100‑1 (MQ=255)
              ttCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAAAAAAAAAAAAAACGCTCACAgg                                                                                       <  2:1091774/100‑1 (MQ=255)
               tCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGc                                                                                      <  1:1811973/100‑1 (MQ=255)
                cGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCt                                                                                     >  1:3807108/1‑100 (MQ=255)
                 ggCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACACTCACAGGCTg                                                                                    >  2:709881/1‑100 (MQ=255)
                  gCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTgc                                                                                   >  1:1852206/1‑100 (MQ=255)
                   cACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTgcg                                                                                  <  2:830238/100‑1 (MQ=255)
                   cACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTgcg                                                                                  <  1:1064725/100‑1 (MQ=255)
                    aCGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTgcgc                                                                                 <  2:3788734/100‑1 (MQ=255)
                     cGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGcc                                                                                <  1:1433820/100‑1 (MQ=255)
                     cGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGcc                                                                                <  1:2946064/100‑1 (MQ=255)
                      gTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCCGAAAAACGCTCAAAGGCTGCGCCg                                                                               >  1:1371224/1‑100 (MQ=255)
                      gTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCg                                                                               >  1:1259540/1‑100 (MQ=255)
                         ttGCCGAAGATCGTCACTGTTGTGCCGGTTCTGGTAAGAGATAACCACACCGCGTGAACGGCGGTAGATCTTAGGAAAACGCGCACAGGCTGCGCCGACg                                                                            <  2:810980/100‑1 (MQ=255)
                         ttGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACg                                                                            <  1:403394/100‑1 (MQ=255)
                          tGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACCCGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACgg                                                                           <  2:2823871/100‑1 (MQ=255)
                           gCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGt                                                                          >  1:2855489/1‑100 (MQ=255)
                            cAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACACCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGtt                                                                         >  2:1044970/1‑100 (MQ=255)
                              gAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTgg                                                                       <  2:436440/100‑1 (MQ=255)
                                atagCGTCCATAGGGTGCCGGTTCTGTTAAGAGATAAACGCGCCGCGTGAACGGCGGTAGAACTCAGAAAAACGCGCGCGGGCTGCGCCGACGGTTgggg                                                                     <  2:325162/100‑1 (MQ=255)
                                 tatCGTCCAGATTGTGCCGGTTCGGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAACCGCTCACAGGCTGCGACGACGGTTGGGGt                                                                    <  2:3338449/100‑1 (MQ=255)
                                        cATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTataaata                                                             <  1:1019924/100‑1 (MQ=255)
                                               tgCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGca                                                      <  2:527475/100‑1 (MQ=255)
                                                 ccGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcatc                                                    <  2:1542970/100‑1 (MQ=255)
                                                  cGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcatca                                                   <  1:3359050/100‑1 (MQ=255)
                                                  cGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcatca                                                   <  1:409423/100‑1 (MQ=255)
                                                   ggTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcatcat                                                  >  1:404563/1‑100 (MQ=255)
                                                   ggTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGcatcat                                                  <  2:3327577/100‑1 (MQ=255)
                                                     tgcTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatct                                                <  1:1937700/98‑1 (MQ=255)
                                                       cTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctca                                              <  1:738948/100‑1 (MQ=255)
                                                        tGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcat                                             >  1:919500/1‑100 (MQ=255)
                                                        tGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcat                                             >  1:725050/1‑100 (MQ=255)
                                                         ggTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatctcatc                                            >  1:1918275/1‑100 (MQ=255)
                                                          gTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCAtcatcttgatg                                           >  1:1451440/1‑95 (MQ=255)
                                                          gTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCtcatca                                           <  1:1664427/100‑1 (MQ=255)
                                                          gTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCtcatca                                           <  1:2535061/100‑1 (MQ=255)
                                                           tAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCaa                                          <  1:1197742/100‑1 (MQ=255)
                                                            aaGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAg                                         <  2:839532/100‑1 (MQ=255)
                                                            aaGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAg                                         >  1:1255998/1‑100 (MQ=255)
                                                             agagATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAga                                        >  1:2372082/1‑100 (MQ=255)
                                                             agagATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAga                                        >  2:4119649/1‑100 (MQ=255)
                                                               agaTAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAgatg                                      <  1:2204390/100‑1 (MQ=255)
                                                                  tAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGAtt                                   <  2:2372068/100‑1 (MQ=255)
                                                                  tAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCAAAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGAtt                                   >  1:344158/1‑100 (MQ=255)
                                                                  tAAACACGCCGCGTGAACGGCGGTAGATCTAAGCAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCAGCAAGATGAtt                                   >  2:2714627/1‑100 (MQ=255)
                                                                     acacGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGtt                                >  1:1481164/1‑100 (MQ=255)
                                                                      cacGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGttt                               <  1:1318803/100‑1 (MQ=255)
                                                                      cacGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGtt                                <  2:3379136/99‑1 (MQ=255)
                                                                       acGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTa                              <  2:1007444/100‑1 (MQ=255)
                                                                        cgccgcGTGAACGTCGGTAGATCTCAGAAAAACGCTCATAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAc                             >  2:2544089/1‑100 (MQ=255)
                                                                        cgccgcGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAc                             >  1:2869647/1‑100 (MQ=255)
                                                                        cgccgcGTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAc                             >  1:2869620/1‑100 (MQ=255)
                                                                        cgccgcGTGAACGGCGGTAGATCTCAGAAAAACGCTCACCGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAc                             <  2:2897944/100‑1 (MQ=255)
                                                                        cgccgcGTGAACGGCGGTAGATCTCAGAAAAACACTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAc                             <  1:976384/100‑1 (MQ=255)
                                                                         gccgcGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAcc                            <  2:638116/100‑1 (MQ=255)
                                                                             cgTGAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGAc                        <  2:2855473/100‑1 (MQ=255)
                                                                              gTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACg                       >  2:1137222/1‑100 (MQ=255)
                                                                               tGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACgg                      <  1:2376179/100‑1 (MQ=255)
                                                                                gAACGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGAGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACgg                      >  2:3254506/1‑99 (MQ=255)
                                                                                 aaCGGCGGTAGATCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTa                    <  1:1973555/100‑1 (MQ=255)
                                                                                 aaCGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTa                    <  1:1498865/100‑1 (MQ=255)
                                                                                  aCGGCGGTAGCTCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATGATCTCATCAAGATGATTGTTTACCAGACGGTag                   <  2:2725143/100‑1 (MQ=255)
                                                                                   cggcggTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTaga                  >  1:142637/1‑100 (MQ=255)
                                                                                   cggcggTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTaga                  >  1:599838/1‑100 (MQ=255)
                                                                                   cggcggTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGCATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTag                   >  1:1498148/1‑99 (MQ=255)
                                                                                     gcggTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTagaag                <  2:1481152/100‑1 (MQ=255)
                                                                                         tAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAggg            <  1:12924/100‑1 (MQ=255)
                                                                                         tAGATCTCAGAAAAACGCTCACAGGCTGCGCCAACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAggg            <  2:321866/100‑1 (MQ=255)
                                                                                          aGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGt           <  1:2051383/100‑1 (MQ=255)
                                                                                            aTCTCAGGAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGttt         >  2:3211243/1‑100 (MQ=255)
                                                                                            aTCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGt           <  1:2051337/98‑1 (MQ=255)
                                                                                             tctcAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTc        >  2:3133190/1‑100 (MQ=255)
                                                                                              ctcAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCg       <  1:3329520/100‑1 (MQ=255)
                                                                                              ctcAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCg       <  1:825751/100‑1 (MQ=255)
                                                                                               tcAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGt      <  1:1943278/100‑1 (MQ=255)
                                                                                               tcAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTAACAGACGATAGAAGAGGGTATc        >  2:4056944/1‑98 (MQ=255)
                                                                                                cAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGtt     >  1:870553/1‑100 (MQ=255)
                                                                                                  gAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAg   <  1:2544103/100‑1 (MQ=255)
                                                                                                  gAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAg   <  1:2043924/100‑1 (MQ=255)
                                                                                                   aaaaaCGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAgt  >  1:3670282/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CTTTAATATTATGGTTCGGCACGTTTTGCAGAATATCGTCCATATTGTGCCGGTTCTGGTAAGAGATAAACACGCCGCGTGAACGGCGGTAGATCTCAGAAAAACGCTCACAGGCTGCGCCGACGGTTGGGGTATAAATAACAGGCATCATCTCATCAAGATGATTGTTTACCAGACGGTAGAAGAGGGTTTCGTTAGT  >  NC_000913/1555225‑1555423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: