Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,674,955 A→T 17.0% intergenic (+8/+17) tqsA → / ← pntB pheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,674,9550AT17.0% 287.8 / 46.8 117intergenic (+8/+17)tqsA/pntBpheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (38/59);  new base T (6/14);  total (44/73)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

TGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGG  >  NC_000913/1674856‑1675053
                                                                                                   |                                                                                                  
caattttGACAATAATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTca                                                                                                    >  1:1071576/6‑100 (MQ=255)
   cattGACCATTATTGTCAAAATTGCGCTTGGACAAACAGCTGGCGGTCAACGCATCTCCGCTCTGTCAAGCGATCTCAATACAGAGTGACGGCCTcagca                                                                                                 <  2:1431965/98‑1 (MQ=255)
   cGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGGCTcagca                                                                                                 <  1:2172686/100‑1 (MQ=255)
   cGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagca                                                                                                 >  1:873395/1‑100 (MQ=255)
   cGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagca                                                                                                 >  1:2834679/1‑100 (MQ=255)
      tGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCagag                                                                                              <  2:161197/100‑1 (MQ=255)
      tGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCagag                                                                                              >  1:3653896/1‑100 (MQ=255)
      tGACAAGTATTATAAAAATTGCGCTTTAACAAACAGCGGGAGAGCAAAGCATCGCCGTTCTGTTAAGCGATCGCAATAAAGAGTGACGGCCTcatcag                                                                                                <  2:3914071/98‑1 (MQ=255)
       gACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAgg                                                                                             <  1:664802/100‑1 (MQ=255)
       gACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAgg                                                                                             <  1:3663102/100‑1 (MQ=255)
       gACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTGCTGTTAAGCGATCACAAAAAAAAGTGAAGGTCTcagcag                                                                                                >  2:2880827/1‑97 (MQ=255)
        aCAATTATTGTCAAACTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGc                                                                                            <  2:2900530/100‑1 (MQ=255)
        aCAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGc                                                                                            <  1:3248031/100‑1 (MQ=255)
         cAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGcc                                                                                           <  1:2594259/100‑1 (MQ=255)
         cAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGcc                                                                                           >  1:1004460/1‑100 (MQ=255)
         cAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGcc                                                                                           <  1:4065674/100‑1 (MQ=255)
         cAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGcc                                                                                           >  1:1443387/1‑100 (MQ=255)
            ttattGTGAAAATTGCGGTGGACCAAACAGCGTGAGTTCAAAGCATCGCCGTTCGGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTc                                                                                        <  2:2995385/100‑1 (MQ=255)
            ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTc                                                                                        <  2:1402081/100‑1 (MQ=255)
            ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTc                                                                                        <  2:1750590/100‑1 (MQ=255)
             tattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCa                                                                                       <  1:2727165/100‑1 (MQ=255)
              attGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                      <  1:1516903/100‑2 (MQ=255)
               ttGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCact                                                                                     <  1:3915333/100‑3 (MQ=255)
               ttGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCa                                                                                       <  1:3107909/98‑1 (MQ=255)
                tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAggg                                                                                    <  1:1025589/100‑1 (MQ=255)
                tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAggg                                                                                    >  1:2495085/1‑100 (MQ=255)
                tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAg                                                                                      >  1:177863/1‑98 (MQ=255)
                 gtaaaaaCTGCGCTTTAACAAACAGCGGGAGGACAAAACATCGCCGGCCTGTTCGGAGATCTCAATAAAGGGTGACGGCCTCTGATGCGGCCGTCact                                                                                     <  2:2807690/95‑3 (MQ=255)
                 gTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCactct                                                                                   <  2:3064979/100‑5 (MQ=39)
                 gTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGt                                                                                   <  1:621260/100‑1 (MQ=255)
                 gTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGt                                                                                   >  2:3081103/1‑100 (MQ=255)
                 gTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGt                                                                                   >  2:3078834/1‑100 (MQ=255)
                  tCAAGATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGtt                                                                                  <  2:3296065/100‑1 (MQ=255)
                  tCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGtt                                                                                  >  1:2004650/1‑100 (MQ=255)
                  tCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGtt                                                                                  <  1:1680503/100‑1 (MQ=255)
                   cAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTa                                                                                 >  1:2344367/1‑100 (MQ=255)
                      aaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACag                                                                              <  2:14463/100‑1 (MQ=255)
                      aaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACag                                                                              <  2:1408236/100‑1 (MQ=255)
                       aTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACaga                                                                             <  2:837557/100‑1 (MQ=255)
                         tGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACagag                                                                            <  2:1894538/99‑1 (MQ=255)
                           cgcTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCtt                                                                         >  2:2671216/1‑100 (MQ=255)
                           cgcTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCtt                                                                         >  2:4098299/1‑100 (MQ=255)
                              ttGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCa                                                                      <  2:1616651/100‑1 (MQ=255)
                                gAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAgg                                                                    <  1:3078853/100‑1 (MQ=255)
                                 aacaaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGa                                                                   >  1:1348195/1‑100 (MQ=255)
                                    aaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTg                                                                <  1:461708/100‑1 (MQ=255)
                                     aacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGc                                                               >  1:3652440/1‑100 (MQ=255)
                                       caGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCAt                                                             >  2:3069249/1‑100 (MQ=255)
                                        aGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATc                                                            <  1:146216/100‑1 (MQ=255)
                                         gCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCGGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATcc                                                           <  2:947988/100‑1 (MQ=255)
                                          cGGGATGTCAAAGCATCGCCGTTCTGTTAAGCGATGTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATcc                                                           <  2:1001083/99‑1 (MQ=255)
                                            ggAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACg                                                        >  2:553327/1‑100 (MQ=255)
                                               ggTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTg                                                     <  1:1021440/100‑1 (MQ=255)
                                                gTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTgg                                                    <  1:2993529/100‑1 (MQ=255)
                                                gTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTg                                                     >  1:245477/1‑99 (MQ=255)
                                                   aaaGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCtt                                                 <  2:2012024/100‑1 (MQ=255)
                                                    aaGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCttt                                                >  1:1723920/1‑100 (MQ=255)
                                                    aaGCATCACCGTTCTGTTAAGCAATCTCAATAAAGAGTGACGGCACCAGCAGAGGCCATCAGTGATACAGAGCTTTCAGAATCGCATCCACgaag                                                     >  2:2398743/1‑92 (MQ=255)
                                                     aGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTg                                               <  2:873389/100‑1 (MQ=255)
                                                     aGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTg                                               <  2:2071353/100‑1 (MQ=255)
                                                       cATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGc                                             <  1:1901852/100‑1 (MQ=255)
                                                        aTCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCg                                            <  1:1775671/100‑1 (MQ=255)
                                                          cGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTc                                          <  1:855297/100‑1 (MQ=255)
                                                            ccGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCAc                                        <  1:1262266/100‑1 (MQ=255)
                                                              gTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCa                                      <  1:2345410/100‑1 (MQ=255)
                                                              gTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTAAGGATTGCATCCACGCTGGCTTTGGCGTCACCa                                      <  1:3078409/100‑1 (MQ=255)
                                                                   tttAACCGATCTTAATAAAGTGTGACGGTCTTATTATAGTCCGTCAGGGTTACAGAGCTTTCACGATTGCTTCCACGCTTGCTTTTCCGTCACCAAACAg                                 <  2:3718705/99‑1 (MQ=255)
                                                                    ttAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGc                                <  1:2931161/100‑1 (MQ=255)
                                                                     tAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCa                               <  2:1233160/100‑1 (MQ=255)
                                                                     tAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCa                               <  1:2153545/100‑1 (MQ=255)
                                                                      aaGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAt                              >  1:2654835/1‑100 (MQ=255)
                                                                        gCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGACAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAtgt                            >  1:1554019/1‑100 (MQ=255)
                                                                         cGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAtgtg                           <  1:103838/100‑1 (MQ=255)
                                                                           aTCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTggg                         <  1:1174068/100‑1 (MQ=255)
                                                                              tcAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGgtgt                      >  1:3614269/1‑100 (MQ=255)
                                                                              tcAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGgtgt                      >  2:2003556/1‑100 (MQ=255)
                                                                                   aaaGAGTGACGGCCTCAGCAGAGGCCGTCAGGGATACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGACGTCACCAAACAGCATGTGGGTGtgtt                   >  2:2485707/1‑98 (MQ=255)
                                                                                    aaGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCt                >  2:3379380/1‑100 (MQ=255)
                                                                                      ccctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg              >  1:3423374/4‑100 (MQ=255)
                                                                                       cctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga             >  2:3383982/3‑100 (MQ=255)
                                                                                       cctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCCGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga             >  1:1225414/3‑100 (MQ=39)
                                                                                       agTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCtt               <  2:1533673/98‑1 (MQ=255)
                                                                                        gTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa            <  2:3629724/100‑1 (MQ=255)
                                                                                        ctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa            >  1:3353231/2‑100 (MQ=255)
                                                                                        ctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa            >  1:1070323/2‑100 (MQ=255)
                                                                                        ctGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa            >  2:309719/2‑100 (MQ=255)
                                                                                        ctGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa            >  2:3525273/2‑100 (MQ=255)
                                                                                         tGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaag           <  2:181159/100‑1 (MQ=255)
                                                                                         tGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaag           <  2:181141/100‑1 (MQ=255)
                                                                                         tGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaag           <  2:2231017/100‑1 (MQ=255)
                                                                                          gACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaaga          >  1:3572632/1‑100 (MQ=255)
                                                                                             ggCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGGATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAAc        <  2:3646651/99‑1 (MQ=255)
                                                                                             ggCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACa       >  2:480921/1‑100 (MQ=255)
                                                                                              gCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAg      <  1:2886129/100‑1 (MQ=255)
                                                                                               ccTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGc     >  2:3122681/1‑100 (MQ=255)
                                                                                               ccTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGc     <  1:2813499/100‑1 (MQ=255)
                                                                                               ccTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTATCCTTGAAGAACAGc     >  1:4065396/1‑100 (MQ=255)
                                                                                                 tCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCgg   >  2:3140289/1‑100 (MQ=255)
                                                                                                 tCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCgg   >  1:2019405/1‑100 (MQ=255)
                                                                                                  cagcagAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCTCGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCggg  <  2:2654819/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
TGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGG  >  NC_000913/1674856‑1675053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: