Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,674,958 A→T 17.7% intergenic (+11/+14) tqsA → / ← pntB pheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,674,9580AT17.7% 277.0 / 48.1 115intergenic (+11/+14)tqsA/pntBpheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (37/57);  new base T (6/14);  total (43/72)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

CGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTT  >  NC_000913/1674859‑1675055
                                                                                                   |                                                                                                 
cattGACCATTATTGTCAAAATTGCGCTTGGACAAACAGCTGGCGGTCAACGCATCTCCGCTCTGTCAAGCGATCTCAATACAGAGTGACGGCCTcagca                                                                                                   <  2:1431965/98‑1 (MQ=255)
cGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGGCTcagca                                                                                                   <  1:2172686/100‑1 (MQ=255)
cGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagca                                                                                                   >  1:873395/1‑100 (MQ=255)
cGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagca                                                                                                   >  1:2947684/1‑100 (MQ=255)
cGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagca                                                                                                   >  1:2834679/1‑100 (MQ=255)
   tGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCagag                                                                                                >  1:3653896/1‑100 (MQ=255)
   tGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCagag                                                                                                <  2:161197/100‑1 (MQ=255)
   tGACAAGTATTATAAAAATTGCGCTTTAACAAACAGCGGGAGAGCAAAGCATCGCCGTTCTGTTAAGCGATCGCAATAAAGAGTGACGGCCTcatcag                                                                                                  <  2:3914071/98‑1 (MQ=255)
    gACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAgg                                                                                               <  1:664802/100‑1 (MQ=255)
    gACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTGCTGTTAAGCGATCACAAAAAAAAGTGAAGGTCTcagcag                                                                                                  >  2:2880827/1‑97 (MQ=255)
     aCAATTATTGTCAAACTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGc                                                                                              <  2:2900530/100‑1 (MQ=255)
      cAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGcc                                                                                             <  1:2594259/100‑1 (MQ=255)
      cAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGcc                                                                                             >  1:1004460/1‑100 (MQ=255)
      cAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGcc                                                                                             <  1:4065674/100‑1 (MQ=255)
      cAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGcc                                                                                             >  1:1443387/1‑100 (MQ=255)
         ttattGTGAAAATTGCGGTGGACCAAACAGCGTGAGTTCAAAGCATCGCCGTTCGGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTc                                                                                          <  2:2995385/100‑1 (MQ=255)
         ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTc                                                                                          <  2:1402081/100‑1 (MQ=255)
         ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTc                                                                                          <  2:1750590/100‑1 (MQ=255)
          tattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCa                                                                                         <  1:2194743/100‑1 (MQ=255)
          tattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCa                                                                                         <  1:2727165/100‑1 (MQ=255)
           attGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                        <  1:1244901/100‑2 (MQ=255)
            ttGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCact                                                                                       <  1:3915333/100‑3 (MQ=255)
            ttGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCa                                                                                         <  1:3107909/98‑1 (MQ=255)
             tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAggg                                                                                      <  1:1025589/100‑1 (MQ=255)
             tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAggg                                                                                      <  1:2745245/100‑1 (MQ=255)
             tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAg                                                                                        >  1:177863/1‑98 (MQ=255)
              gtaaaaaCTGCGCTTTAACAAACAGCGGGAGGACAAAACATCGCCGGCCTGTTCGGAGATCTCAATAAAGGGTGACGGCCTCTGATGCGGCCGTCact                                                                                       <  2:2807690/95‑3 (MQ=255)
              gTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCactct                                                                                     <  2:3064979/100‑5 (MQ=39)
              gTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGt                                                                                     <  1:621260/100‑1 (MQ=255)
              gTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGt                                                                                     >  2:3081103/1‑100 (MQ=255)
              gTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGt                                                                                     >  2:3078834/1‑100 (MQ=255)
               tCAAGATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGtt                                                                                    <  2:3296065/100‑1 (MQ=255)
               tCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGtt                                                                                    <  1:1680503/100‑1 (MQ=255)
                   aaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACag                                                                                <  2:14463/100‑1 (MQ=255)
                   aaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACag                                                                                <  2:1408236/100‑1 (MQ=255)
                    aTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACaga                                                                               <  2:837557/100‑1 (MQ=255)
                      tGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACagag                                                                              <  2:1894538/99‑1 (MQ=255)
                        cgcTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCtt                                                                           >  2:2671216/1‑100 (MQ=255)
                        cgcTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCtt                                                                           >  2:4098299/1‑100 (MQ=255)
                           ttGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCa                                                                        <  2:1616651/100‑1 (MQ=255)
                             gAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAgg                                                                      <  1:3078853/100‑1 (MQ=255)
                              aacaaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGa                                                                     >  1:1348195/1‑100 (MQ=255)
                                 aaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTg                                                                  <  1:461708/100‑1 (MQ=255)
                                  aacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGc                                                                 >  1:3652440/1‑100 (MQ=255)
                                    caGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCAt                                                               >  2:3069249/1‑100 (MQ=255)
                                     aGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATc                                                              <  1:146216/100‑1 (MQ=255)
                                     aGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATc                                                              <  1:1513796/100‑1 (MQ=255)
                                      gCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCGGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATcc                                                             <  2:947988/100‑1 (MQ=255)
                                       cGGGATGTCAAAGCATCGCCGTTCTGTTAAGCGATGTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATcc                                                             <  2:1001083/99‑1 (MQ=255)
                                         ggAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACg                                                          >  2:553327/1‑100 (MQ=255)
                                            ggTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTg                                                       <  1:1021440/100‑1 (MQ=255)
                                             gTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTgg                                                      >  1:2481191/1‑100 (MQ=255)
                                             gTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTg                                                       >  1:245477/1‑99 (MQ=255)
                                               cAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCt                                                    <  1:3440371/100‑1 (MQ=255)
                                                aaaGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCtt                                                   <  2:2012024/100‑1 (MQ=255)
                                                 aaGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCttt                                                  >  1:3660405/1‑100 (MQ=255)
                                                 aaGCATCACCGTTCTGTTAAGCAATCTCAATAAAGAGTGACGGCACCAGCAGAGGCCATCAGTGATACAGAGCTTTCAGAATCGCATCCACgaag                                                       >  2:2398743/1‑92 (MQ=255)
                                                  aGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTg                                                 <  2:873389/100‑1 (MQ=255)
                                                  aGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTg                                                 <  2:2071353/100‑1 (MQ=255)
                                                    cATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGc                                               <  1:1901852/100‑1 (MQ=255)
                                                     aTCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCg                                              <  1:17179/100‑1 (MQ=255)
                                                     aTCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCg                                              <  1:1775671/100‑1 (MQ=255)
                                                       cGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTc                                            <  1:855297/100‑1 (MQ=255)
                                                           gTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCa                                        <  1:2345410/100‑1 (MQ=255)
                                                           gTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTAAGGATTGCATCCACGCTGGCTTTGGCGTCACCa                                        <  1:3078409/100‑1 (MQ=255)
                                                                tttAACCGATCTTAATAAAGTGTGACGGTCTTATTATAGTCCGTCAGGGTTACAGAGCTTTCACGATTGCTTCCACGCTTGCTTTTCCGTCACCAAACAg                                   <  2:3718705/99‑1 (MQ=255)
                                                                 ttAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGc                                  <  1:2931161/100‑1 (MQ=255)
                                                                  tAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCa                                 <  1:2153545/100‑1 (MQ=255)
                                                                  tAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCa                                 <  2:1233160/100‑1 (MQ=255)
                                                                   aaGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAt                                >  1:2654835/1‑100 (MQ=255)
                                                                     gCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGACAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAtgt                              >  1:1554019/1‑100 (MQ=255)
                                                                      cGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAtgtg                             <  1:103838/100‑1 (MQ=255)
                                                                        aTCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTggg                           <  1:1174068/100‑1 (MQ=255)
                                                                           tcAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGgtgt                        >  2:2003556/1‑100 (MQ=255)
                                                                           tcAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGgtgt                        >  1:3614269/1‑100 (MQ=255)
                                                                                aaaGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTcc                   <  1:1983183/100‑1 (MQ=255)
                                                                                aaaGAGTGACGGCCTCAGCAGAGGCCGTCAGGGATACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGACGTCACCAAACAGCATGTGGGTGtgtt                     >  2:2485707/1‑98 (MQ=255)
                                                                                 aaGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCt                  >  2:3379380/1‑100 (MQ=255)
                                                                                   ccctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg                >  1:3423374/4‑100 (MQ=255)
                                                                                    cctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga               >  2:3383982/3‑100 (MQ=255)
                                                                                    cctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCCGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga               >  1:1225414/3‑100 (MQ=39)
                                                                                    agTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCtt                 <  2:1533673/98‑1 (MQ=255)
                                                                                     gTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa              <  2:3629724/100‑1 (MQ=255)
                                                                                     ctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa              >  1:3353231/2‑100 (MQ=255)
                                                                                     ctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa              >  1:1070323/2‑100 (MQ=255)
                                                                                     ctGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa              >  2:309719/2‑100 (MQ=255)
                                                                                     ctGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa              >  2:3525273/2‑100 (MQ=255)
                                                                                      tGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaag             <  1:3216512/100‑1 (MQ=255)
                                                                                      tGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaag             <  2:181141/100‑1 (MQ=255)
                                                                                      tGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaag             <  2:181159/100‑1 (MQ=255)
                                                                                      tGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaag             <  2:2231017/100‑1 (MQ=255)
                                                                                          ggCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGGATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAAc          <  2:3646651/99‑1 (MQ=255)
                                                                                          ggCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACa         >  2:480921/1‑100 (MQ=255)
                                                                                           gCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAg        <  1:2886129/100‑1 (MQ=255)
                                                                                            ccTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGc       >  2:3122681/1‑100 (MQ=255)
                                                                                            ccTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTATCCTTGAAGAACAGc       >  1:4065396/1‑100 (MQ=255)
                                                                                              tCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCgg     >  2:3140289/1‑100 (MQ=255)
                                                                                              tCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCgg     >  1:2019405/1‑100 (MQ=255)
                                                                                               cagcagAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCTCGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCggg    <  2:2654819/100‑1 (MQ=255)
                                                                                                 gcagAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGtt  >  1:420935/1‑100 (MQ=255)
                                                                                                   |                                                                                                 
CGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTT  >  NC_000913/1674859‑1675055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: