Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,681,932 T→C 100% P33P (CCA→CCG)  ydgC ← GlpM family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,681,9320TC96.0% 352.5 / 0.9 111P33P (CCA→CCG) ydgCGlpM family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base C (45/61);  total (48/63)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTACCAGCC  >  NC_000913/1681835‑1682026
                                                                                                 |                                                                                              
tCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCgg                                                                                              <  1:2321964/100‑1 (MQ=255)
  naCCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGaa                                                                                            >  2:1520367/2‑100 (MQ=255)
  gACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGaa                                                                                            <  1:3652285/100‑1 (MQ=255)
   aCCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAAt                                                                                           >  1:318348/1‑100 (MQ=255)
    ccACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCCCGTTCGCTGGCACGAATATAATGCGCGATAAGCGCAAAGGTCGGGCAAAGCGGaa                                                                                            >  2:3227758/1‑98 (MQ=255)
       caTACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGc                                                                                       >  2:1396727/1‑100 (MQ=255)
         tACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGccc                                                                                     <  1:2566615/100‑1 (MQ=255)
          aCTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGAAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGccgg                                                                                    >  1:2462422/1‑98 (MQ=255)
           cTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATCAGCCCgg                                                                                   >  2:3670385/1‑100 (MQ=255)
           cTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCACGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCgg                                                                                   >  1:542855/1‑100 (MQ=255)
            tAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGc                                                                                  >  1:862351/1‑100 (MQ=255)
            tAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGc                                                                                  >  1:2634744/1‑100 (MQ=255)
             aaaaaTGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCg                                                                                 >  1:2722393/1‑100 (MQ=255)
                aaTGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGata                                                                              >  1:1717164/1‑100 (MQ=255)
                  tgatgGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAGGCGGAATCAGCCCGGCGAtata                                                                            <  2:1657290/100‑1 (MQ=255)
                   gatgGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATCAGCCCGGCGATataa                                                                           <  1:3136980/100‑1 (MQ=255)
                   gatgGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATataa                                                                           <  1:87973/100‑1 (MQ=255)
                   gatgGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATataa                                                                           <  2:2268940/100‑1 (MQ=255)
                   gatgGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATataa                                                                           >  2:2077161/1‑100 (MQ=255)
                    atgGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATataat                                                                          <  1:457447/100‑1 (MQ=255)
                    atgGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATataat                                                                          <  2:3752300/100‑1 (MQ=255)
                       gTTGCGCGTAAGGCTTCAATGCTGCGTTCGCTGGCAATAATATAATGCGCGATAAGCGAAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGAaataataat                                                                       <  2:2793125/100‑1 (MQ=255)
                       gTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATaataat                                                                       >  1:2930914/1‑100 (MQ=255)
                           cgcgTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAAttttt                                                                   <  2:602772/100‑1 (MQ=255)
                           cgcgTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAAGGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAAttttt                                                                   <  2:3373274/100‑1 (MQ=255)
                            gcgTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTc                                                                  <  1:3784621/100‑1 (MQ=255)
                             cgTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCg                                                                 <  2:598990/100‑1 (MQ=255)
                             cgTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCg                                                                 >  2:3348315/1‑100 (MQ=255)
                             cgTAAGGATTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCg                                                                 <  2:4082291/100‑1 (MQ=255)
                               tAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGtt                                                               >  1:2246029/1‑100 (MQ=255)
                                aaGGCTTCAATGCCGCGTTCGCTTGCAACAATATAATGGGGGATAAGCGCACAGATCGTGACAAGTGGAATGAGCGCGGCGATATAATAATTTTTCGtt                                                               <  2:2240657/99‑1 (MQ=255)
                                  ggCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGttttt                                                            <  2:1178069/100‑1 (MQ=255)
                                  ggCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGttttt                                                            >  2:3405464/1‑100 (MQ=255)
                                   gCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTg                                                           >  1:2168428/1‑100 (MQ=255)
                                    cTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTgn                                                          <  1:2442790/100‑2 (MQ=255)
                                    cTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGc                                                          <  1:2979846/100‑1 (MQ=255)
                                    cTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGc                                                          >  2:3537061/1‑100 (MQ=255)
                                    cTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGc                                                          <  1:1708105/100‑1 (MQ=255)
                                    cTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGc                                                          <  2:2673741/100‑1 (MQ=255)
                                    cTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGc                                                          >  2:2444273/1‑100 (MQ=255)
                                     ttCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCt                                                         >  2:1998715/1‑100 (MQ=255)
                                      tCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTa                                                        <  2:2325739/100‑1 (MQ=255)
                                      tCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTa                                                        >  2:1275302/1‑100 (MQ=255)
                                        aaTGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTaaa                                                      >  1:2107191/1‑100 (MQ=255)
                                         aTGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTaaaa                                                     <  2:160258/100‑1 (MQ=255)
                                         aTGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTaaaa                                                     >  2:2108345/1‑100 (MQ=255)
                                          tGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAac                                                    <  1:1750086/100‑1 (MQ=255)
                                          tGCCGCGTTCGCTAGCAACAAAAGAATGCGCGATCAGCGCAAAGGTCGGGAAAAGCGGACTCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAac                                                    <  2:4102212/100‑1 (MQ=255)
                                           gCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAaca                                                   >  2:1418005/1‑100 (MQ=255)
                                            ccGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAacac                                                  >  2:1922236/1‑100 (MQ=255)
                                             cgcgTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACAcc                                                 <  1:3235322/100‑1 (MQ=255)
                                             cgcgTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACAcc                                                 <  2:3971395/100‑1 (MQ=255)
                                              gcgTTGGCAGACAACAATATAATCCTCGATAAGCGAAAACGTCTGAAAAAGCGTAACCAGCCCGTCGATATAATAATTTTTCGTTTTTACTaaa                                                      <  2:2087680/94‑1 (MQ=255)
                                              gcgTTCGCGGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCa                                                <  2:2458056/100‑1 (MQ=255)
                                                    gCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAAc                                          <  2:1526450/100‑1 (MQ=255)
                                                     cTGGCAACAATATAATGCGCGATAAGCGCAAATGTTGGGAAAAGCGGAATCGGTCCGGCGATATAATTATTTTTCGTTTTTTCTAAAACACCAATCAACa                                         <  1:2408934/100‑1 (MQ=255)
                                                     cTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACa                                         >  1:1269150/1‑100 (MQ=255)
                                                       ggCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGc                                       >  1:1398120/1‑100 (MQ=255)
                                                       ggCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGc                                       <  2:1084530/100‑1 (MQ=255)
                                                       ggCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGc                                       <  2:3823228/100‑1 (MQ=255)
                                                       ggCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGc                                       <  1:1000854/100‑1 (MQ=255)
                                                        gCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCa                                      <  2:80771/100‑1 (MQ=255)
                                                         caacaaTATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCAc                                     >  2:1354887/1‑100 (MQ=255)
                                                             aaTATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTAcc                                 >  2:3230110/1‑100 (MQ=255)
                                                             aaTATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTAcc                                 <  2:2772976/100‑1 (MQ=255)
                                                             aaTATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTAcc                                 <  1:488893/100‑1 (MQ=255)
                                                             aaTAGAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTAcc                                 <  2:1730418/100‑1 (MQ=255)
                                                              ataaAATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGAGATAATAATTTTTAGTTTTTGCTAAACCACCAATCAACAGGACTACCa                                >  2:1008215/1‑100 (MQ=255)
                                                               tataATGCGCGAGAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGTGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAg                               <  2:2436960/100‑1 (MQ=255)
                                                                ataATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAgc                              <  1:3235208/100‑1 (MQ=255)
                                                                ataATGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAgc                              <  2:1297174/100‑1 (MQ=255)
                                                                   aTGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTTCTTAAACACCAATCAACAGCACTACCAGCGCa                           >  2:1810017/1‑100 (MQ=255)
                                                                    tGCGCGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCAc                          >  1:486246/1‑100 (MQ=255)
                                                                     gcgcGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCAcc                         >  1:1891751/1‑100 (MQ=255)
                                                                     gcgcGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCAcc                         >  1:2764184/1‑100 (MQ=255)
                                                                     gcgcGATAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCAcc                         >  1:2926064/1‑100 (MQ=255)
                                                                           tAAGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAgcgc                   <  2:2116757/100‑1 (MQ=255)
                                                                            aaGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGcgcg                  <  1:411015/100‑1 (MQ=255)
                                                                            aaGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCCATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAgcgc                   <  2:2011512/99‑1 (MQ=255)
                                                                             aGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGTACTACCAGCGCACCGAGcgcg                  >  2:2584349/1‑99 (MQ=255)
                                                                             aGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCgg                 >  2:2442153/1‑100 (MQ=255)
                                                                             aGCGCAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCgg                 <  2:676501/100‑1 (MQ=255)
                                                                              gcgcAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGc                >  1:846744/1‑100 (MQ=255)
                                                                               cgcAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCt               >  1:2871409/1‑100 (MQ=255)
                                                                               cgaaaaGGGAGGGAAAAGCAGAATCAGCACGGCGATAGAATCATGGTAGGTTTTTGCTAAAACCCCAATCAACAGGACTACCAGCGCACTGGGAGCggg                <  2:415983/96‑2 (MQ=255)
                                                                                 cAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCttt             >  1:506857/1‑100 (MQ=255)
                                                                                 cAAAGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTGTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCttt             <  2:469393/100‑1 (MQ=255)
                                                                                  aaaGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTGTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTg            <  1:1208491/100‑1 (MQ=255)
                                                                                   aaGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGa           <  1:2558013/100‑1 (MQ=255)
                                                                                    aGGTGGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGAt          <  1:993433/100‑1 (MQ=255)
                                                                                    aGGTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGAt          <  1:4014399/100‑1 (MQ=255)
                                                                                     ggTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGAtt         <  1:694155/100‑1 (MQ=255)
                                                                                     ggTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTACGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGAtt         >  2:2259086/1‑100 (MQ=255)
                                                                                      gTCGGGATAATCGGAATCAGCCCGGCGATAGAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTa        <  1:2405661/100‑1 (MQ=255)
                                                                                      gTCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTa        >  1:1245486/1‑100 (MQ=255)
                                                                                       tCGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTAc       >  1:368836/1‑100 (MQ=255)
                                                                                        cGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTAcc      <  1:1987021/100‑1 (MQ=255)
                                                                                        cGGGAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTAcc      >  2:1987008/1‑100 (MQ=255)
                                                                                         gggAAAAGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTACCa     <  2:2576486/100‑1 (MQ=255)
                                                                                            aaaaGCGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTACCAGcc  <  1:2442167/100‑1 (MQ=255)
                                                                                                 |                                                                                              
TCGACCACATACTAAAAATGATGGTTGCGCGTAAGGCTTCAATGCCGCGTTCGCTGGCAACAATATAATGCGCGATAAGCGCAAAGGTCGGGAAAAGTGGAATCAGCCCGGCGATATAATAATTTTTCGTTTTTGCTAAAACACCAATCAACAGCACTACCAGCGCACCGAGCGCGGCTTTGATTACCAGCC  >  NC_000913/1681835‑1682026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑