Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,757,688 T→A 19.5% intergenic (+31/+33) lpp → / ← ldtE murein lipoprotein/murein L,D‑transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,757,6880TA19.5% 264.1 / 61.0 123intergenic (+31/+33)lpp/ldtEmurein lipoprotein/murein L,D‑transpeptidase
Reads supporting (aligned to +/- strand):  ref base T (49/50);  new base A (12/12);  total (61/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01

Only 100 of 123 total aligned reads displayed.

TGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAG  >  NC_000913/1757591‑1757785
                                                                                                 |                                                                                                  
tgctAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACAAtg                                                                                                  <  1:1709405/100‑1 (MQ=255)
  ctAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAACATGGCGCACATtgtg                                                                                                >  1:3764902/1‑100 (MQ=255)
  ctAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATtgtg                                                                                                >  1:2382075/1‑100 (MQ=255)
   tAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTgc                                                                                               >  2:774468/1‑100 (MQ=255)
      aGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACAATGTGCGcc                                                                                            <  1:1578398/100‑1 (MQ=255)
        aTGACGCAGCTCGTGCTAACCAGGGTATGGACAACATGGATACTACATACCGCAAGTAATAGTACCTGTGAAGTGAAGAATGGCGCACAATGTGCGCCAt                                                                                         <  2:3650219/100‑1 (MQ=255)
        aTGACGCAGCTCGTGCTAACCAGCTTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACAATGTGCGCCAt                                                                                         <  2:3971286/100‑1 (MQ=255)
        aTGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACAATGTGCGCCAt                                                                                         <  1:1109248/100‑1 (MQ=255)
         tGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACAATGTGCGCCAtt                                                                                        <  1:3916613/100‑1 (MQ=255)
          gACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCAttt                                                                                       >  1:3876753/1‑100 (MQ=255)
           aCGCAGCTCGTGCTAACCAGCGTCTTGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACAATGTGCGCCAtttt                                                                                      <  2:1556537/100‑1 (MQ=255)
           aCGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCAtttt                                                                                      <  2:2991245/100‑1 (MQ=255)
           aCGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACAATGTGCGCCAtttt                                                                                      <  1:3158261/100‑1 (MQ=255)
           aCGCAGCTCGTGCTAACCAGCGTATGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACAATGTGCGCCAtt                                                                                        <  2:1465476/98‑1 (MQ=255)
            cGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGTGCACATTGTGCGCCAttttt                                                                                     >  1:852595/1‑100 (MQ=255)
             gCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACAATGTGCGCCAtttttc                                                                                    <  2:539701/100‑2 (MQ=255)
               aGCTCGTGCTAACCAGGGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACAATGTGCGCCAtttttcac                                                                                  <  2:1976643/100‑4 (MQ=255)
               aGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCAtttttttt                                                                                  <  1:3592451/100‑1 (MQ=255)
               aGCTCGTGCTAACCAGCGTCTAGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCAtttttttt                                                                                  <  1:2858135/100‑1 (MQ=255)
                gCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTg                                                                                 <  2:514940/100‑1 (MQ=255)
                gCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTg                                                                                 >  1:2552515/1‑100 (MQ=255)
                   cGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAATTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCt                                                                              <  1:2469574/100‑1 (MQ=255)
                     tGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGc                                                                            <  1:1898517/100‑1 (MQ=255)
                      gCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGcc                                                                           >  2:2973062/1‑100 (MQ=255)
                      gCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTCAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCg                                                                          >  2:2839143/1‑100 (MQ=255)
                        tAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGt                                                                         <  1:2160769/100‑1 (MQ=255)
                        tAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGt                                                                         <  1:1872801/100‑1 (MQ=255)
                        tAACCAGCGTCTGAACAACATGGATACTAAATACCGCATGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGt                                                                         <  2:129069/100‑1 (MQ=255)
                         aaCCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGtt                                                                        >  2:3125413/1‑100 (MQ=255)
                          aCCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGttt                                                                       >  1:2154392/1‑100 (MQ=255)
                           ccAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTa                                                                      >  1:730799/1‑100 (MQ=255)
                           ccAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTa                                                                      >  1:1053850/1‑100 (MQ=255)
                              gCGTCTGGACAACATCGCTACTAAAGACCGCAAGTAATAGTACCTATGAAGTGAAAACTGGCGCACATAGTGCGCCATTTTTTATGTCTGCCGTCTAcc                                                                    >  2:3174983/1‑99 (MQ=255)
                               cGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGc                                                                  <  1:3197798/100‑1 (MQ=255)
                                gTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCt                                                                 >  2:3858603/1‑100 (MQ=255)
                                 tCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTAc                                                               >  2:515776/1‑100 (MQ=255)
                                   tGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACt                                                              <  1:726120/100‑1 (MQ=255)
                                   tGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTATGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACt                                                              >  2:1952837/1‑100 (MQ=255)
                                    ggACAACATGGCTACTGAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTg                                                             <  2:2410073/100‑1 (MQ=255)
                                    ggACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTg                                                             >  1:804118/1‑100 (MQ=255)
                                        aacaTGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTc                                                         <  2:3287411/100‑1 (MQ=255)
                                        aacaTGGCTACTAAATACCGCAAGTAATAGGACCTGTGAAGTGAAAAATGGCGCACATTGGGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCg                                                           <  2:1831103/98‑1 (MQ=255)
                                         acaTGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATCGTGCGCCATTTTTTTTTTTTTTTTTTTACCGCTACTGGGt                                                          >  2:2898387/1‑98 (MQ=37)
                                          caTGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCAc                                                       <  2:47493/100‑1 (MQ=255)
                                          caTGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCAc                                                       <  2:156019/100‑1 (MQ=255)
                                               ctactaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTa                                                  >  1:223634/1‑100 (MQ=255)
                                               ctactaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTa                                                  >  2:3610947/1‑100 (MQ=255)
                                                 actaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAAc                                                <  1:4129903/100‑1 (MQ=255)
                                                 actaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAAc                                                <  1:1934397/100‑1 (MQ=255)
                                                 actaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAAc                                                <  1:296625/100‑1 (MQ=255)
                                                 actaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAAc                                                >  1:3271336/1‑100 (MQ=255)
                                                  ctaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTaa                                                 <  1:1910782/98‑1 (MQ=255)
                                                   taAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACat                                              >  1:4028836/1‑100 (MQ=255)
                                                      aTACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATAtt                                           >  1:1234113/1‑100 (MQ=255)
                                                          cGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCt                                       >  2:311134/1‑100 (MQ=255)
                                                          cGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCt                                       >  2:126636/1‑100 (MQ=255)
                                                          cGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCt                                       >  2:2074632/1‑100 (MQ=255)
                                                           gCAAGTAATAGTACCTGTGAAGTGGAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTcc                                         <  2:3482751/97‑1 (MQ=255)
                                                           gCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCtt                                      >  1:2894648/1‑100 (MQ=255)
                                                            cAAGTAATAGTACCTGTGAAGTGAAAAATGGCTCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTg                                     <  1:3812534/100‑1 (MQ=255)
                                                             aaGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGc                                    <  2:255867/100‑1 (MQ=255)
                                                                 aaTAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTg                                <  2:293260/100‑1 (MQ=255)
                                                                   tAGTACCTTTGAAGTTACAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTTCCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGt                              <  2:3319163/100‑1 (MQ=255)
                                                                    aGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGtt                             <  2:1939209/100‑1 (MQ=255)
                                                                     gTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTc                            <  2:4095686/100‑1 (MQ=255)
                                                                     gTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTc                            <  2:3974592/100‑1 (MQ=255)
                                                                     gTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTc                            <  1:1188141/100‑1 (MQ=255)
                                                                       aCCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCa                           <  2:3251981/99‑1 (MQ=255)
                                                                       aCCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCAc                          >  1:2267109/1‑100 (MQ=255)
                                                                       aCCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCAc                          <  1:317160/100‑1 (MQ=255)
                                                                        ccTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCAcc                         >  1:2905985/1‑100 (MQ=255)
                                                                         cTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCa                        <  1:1326959/100‑1 (MQ=255)
                                                                          tgtgAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCTCCAt                       >  2:3072612/1‑100 (MQ=255)
                                                                          tgtgAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCAt                       >  1:1565808/1‑100 (MQ=255)
                                                                          tgtgAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCAt                       >  2:2617504/1‑100 (MQ=255)
                                                                          tgtgAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCAt                       >  1:3530544/1‑100 (MQ=255)
                                                                             gaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCt                    <  2:738898/100‑1 (MQ=255)
                                                                              aagtgaaAAATGGCGCACATTGTGCGCCATTTGTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTg                   <  2:3727302/100‑1 (MQ=255)
                                                                                aaaaaaaaTGGCGCACAATGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTgcg                 >  1:2593121/4‑100 (MQ=255)
                                                                                aaaaaaaaTGGCGCACAATGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTgc                  >  1:335193/4‑99 (MQ=255)
                                                                                 tgaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTgcgc                >  1:1870100/1‑100 (MQ=255)
                                                                                 aaaaaaaTGGCGCACAATGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTgcgc                >  1:1976221/3‑100 (MQ=255)
                                                                                 aaaaaaaTGGCGCACAATGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTgcgc                >  1:1763823/3‑100 (MQ=255)
                                                                                 aaaaaaaTGGCGCACAATGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTgcgc                >  1:1125217/3‑100 (MQ=255)
                                                                                 aaaaaaaTGGCGCACAATGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTgcgc                >  2:61919/3‑100 (MQ=255)
                                                                                 aaaaaaaTGGCGCACAATGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTgcgc                >  2:1115697/3‑100 (MQ=255)
                                                                                  gaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCt               <  1:3778568/100‑1 (MQ=255)
                                                                                  gaaAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCt               <  2:1585240/100‑1 (MQ=255)
                                                                                   aaaaaTGGCGCACATTGTGCGCCATTTTTTTTTTCTGCCGTTTACCGCTACTGCGTCACTCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTg              <  2:3640176/100‑1 (MQ=255)
                                                                                    aaaaTGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGa             >  1:1789465/1‑100 (MQ=255)
                                                                                     aaaTGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGAc            >  1:3478926/1‑100 (MQ=255)
                                                                                       aTGGCGCACAATGTGCGCCATTTTTTTTTTTTTTCCGTTTCCAGCTCCTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCTCTGAct           >  2:2446462/1‑100 (MQ=255)
                                                                                       aTGGCGCACAATGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGActc          >  1:1126089/1‑100 (MQ=255)
                                                                                       aTGGCGCACAATGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGActc          >  1:520072/1‑100 (MQ=255)
                                                                                       aTGGCGCACAATGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGActc          >  1:409080/1‑100 (MQ=255)
                                                                                        tGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGActct         <  1:2744029/100‑1 (MQ=255)
                                                                                           cgcTCATTTTGCTTCATTTTTTTTTTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTAc       <  1:341668/99‑1 (MQ=255)
                                                                                            gcACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTg     >  2:1683219/1‑100 (MQ=255)
                                                                                             cacaTTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTGCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGa    >  2:786289/1‑100 (MQ=255)
                                                                                               caTTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAg  <  1:544845/100‑1 (MQ=255)
                                                                                                 |                                                                                                  
TGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAG  >  NC_000913/1757591‑1757785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: