Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,764,687 T→C 16.6% intergenic (‑301/+247) sufA ← / ← ydiH Fe‑S cluster assembly protein/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,764,6870TC16.6% 326.1 / 52.7 146intergenic (‑301/+247)sufA/ydiHFe‑S cluster assembly protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (56/65);  new base C (5/19);  total (62/84)
Fisher's exact test for biased strand distribution p-value = 2.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

Only 100 of 153 total aligned reads displayed.

GCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATA  >  NC_000913/1764588‑1764786
                                                                                                   |                                                                                                    
gCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGt                                                                                                      >  1:3767874/1‑100 (MQ=255)
gCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGt                                                                                                      >  1:4099439/1‑100 (MQ=255)
gCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGt                                                                                                      >  2:3085917/1‑100 (MQ=255)
gCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGt                                                                                                      >  2:1215247/1‑100 (MQ=255)
  aTTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCt                                                                                                    >  2:3529780/1‑100 (MQ=255)
  aTTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCt                                                                                                    >  2:2967513/1‑100 (MQ=255)
     aaGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCtgtg                                                                                                 <  1:3752590/100‑1 (MQ=255)
     aaGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCtgtg                                                                                                 >  1:1116431/1‑100 (MQ=255)
       ggCTGTGATGAAAAAGACAAAGTTGTATTGAAAATGACTATTGAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCg                                                                                              >  2:2185660/1‑100 (MQ=255)
       ggCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCg                                                                                              >  1:1021603/1‑100 (MQ=255)
         cTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGac                                                                                            <  1:3901470/100‑1 (MQ=255)
          cgatctgaAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCtgtgtcgca                                                                                            <  2:1402757/95‑6 (MQ=255)
           gtgATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGacac                                                                                          >  1:3325466/1‑100 (MQ=255)
           gtgATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGacac                                                                                          >  2:555659/1‑100 (MQ=255)
           gtgATGAAAAAGAAAAAGTTGTATTGAAAATGACGATTTAAGAGATAGGTAAAAAAGTGCAGAGTGCAGAAATAAGAAAACCCTTAAGTCTGTGCGaca                                                                                           >  2:2156152/1‑99 (MQ=255)
              atgaAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAgg                                                                                       >  2:1097577/1‑100 (MQ=255)
                gaaaaagaaaaaGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCt                                                                                     <  2:3076488/100‑1 (MQ=255)
                gaaaaagaaaaaGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCt                                                                                     >  1:3844400/1‑100 (MQ=255)
                 aaaaagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCtt                                                                                    >  1:1827336/1‑100 (MQ=255)
                 aaaaagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCtt                                                                                    >  1:439917/1‑100 (MQ=255)
                     agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAgg                                                                                >  2:3895237/1‑100 (MQ=255)
                     agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAg                                                                                 >  2:1067539/1‑99 (MQ=255)
                     agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAgg                                                                                <  2:1724689/100‑1 (MQ=37)
                     agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAgg                                                                                <  1:2202584/100‑1 (MQ=37)
                     agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAgg                                                                                <  1:2209049/100‑1 (MQ=37)
                     agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAgg                                                                                <  1:1254989/100‑1 (MQ=37)
                      gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAggg                                                                               <  1:511569/100‑1 (MQ=255)
                      gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAggg                                                                               <  1:1420421/100‑1 (MQ=255)
                      gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAggg                                                                               <  1:4108583/100‑1 (MQ=37)
                      gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAggg                                                                               <  1:315721/100‑1 (MQ=37)
                      gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAggg                                                                               <  2:966693/100‑1 (MQ=37)
                      gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAggg                                                                               <  1:2817859/100‑1 (MQ=37)
                       aaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGt                                                                              >  1:3289558/1‑100 (MQ=255)
                       aaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAGGGt                                                                              <  2:855889/100‑1 (MQ=37)
                        aaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAGGGtt                                                                             <  2:1880168/100‑1 (MQ=37)
                          aagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTc                                                                           <  1:1518530/100‑1 (MQ=255)
                           agTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCt                                                                          >  1:3378210/1‑100 (MQ=255)
                           agTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAGGGttttc                                                                          <  1:1312541/100‑3 (MQ=25)
                              tGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTAcc                                                                       <  1:1671004/100‑1 (MQ=255)
                              tGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTAcc                                                                       <  1:3581797/100‑1 (MQ=255)
                              tGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGACTGTGCGACACAGGCTTAAGGGTTTCTa                                                                         >  2:3257366/1‑98 (MQ=255)
                               gTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTAccc                                                                      <  1:2567659/100‑1 (MQ=255)
                               gTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGACTTAAGGGTTTCTAccc                                                                      >  2:2481555/1‑100 (MQ=255)
                                 aTTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCa                                                                    >  2:2753471/1‑100 (MQ=255)
                                  ttGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCAt                                                                   <  1:2339274/100‑1 (MQ=255)
                                  ttGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCAt                                                                   >  1:2384582/1‑100 (MQ=255)
                                    gAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATcc                                                                 >  1:1937933/1‑100 (MQ=255)
                                    gAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATcc                                                                 >  2:1038255/1‑100 (MQ=255)
                                         tGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGgcgc                                                            <  1:2395427/100‑1 (MQ=255)
                                           aCTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCtt                                                          >  2:83226/1‑100 (MQ=255)
                                           aCTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCtt                                                          <  1:1843686/100‑1 (MQ=255)
                                                 tAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTcc                                                    <  1:1242830/100‑1 (MQ=255)
                                                  aaGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCAACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCg                                                   >  2:2642381/1‑100 (MQ=255)
                                                   agagATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCgg                                                  <  2:2995994/100‑1 (MQ=255)
                                                   agagATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCgg                                                  <  1:2552158/100‑1 (MQ=255)
                                                         aGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCActct                                            >  2:4103933/1‑100 (MQ=255)
                                                         aGGTAAAAAAGTGCAGCGGTCAGAACTAAGAAAACCCTGACGCCTGTGCGACACAGGCTTAAGGGTTTCTACCGCAGCCGGCGCTTATCTCCGGCActc                                             <  2:1491415/99‑1 (MQ=255)
                                                          ggTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACtctc                                           <  1:3085634/100‑1 (MQ=255)
                                                          ggTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACtctc                                           <  1:785438/100‑1 (MQ=255)
                                                           gTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCa                                          >  1:1869833/1‑100 (MQ=255)
                                                             aaaaaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGt                                        <  1:1871472/100‑1 (MQ=255)
                                                              aaaaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTg                                       <  1:1012232/100‑1 (MQ=255)
                                                              aaaaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTg                                       <  2:1174563/100‑1 (MQ=255)
                                                               aaaaGTGCAGCGTTCAGGAATAAGAAAACCCTTAAGTCTTTGCGACACAGGCTTAGGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTg                                       <  2:2566238/99‑1 (MQ=255)
                                                                 aaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCt                                    >  2:2004472/1‑100 (MQ=255)
                                                                 aaGTGCAGCGTTCAGAAATAAGAAAACCCTACAGTCTGCGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGc                                     <  2:2902401/99‑1 (MQ=255)
                                                                  aGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCtt                                   <  2:1528969/100‑1 (MQ=255)
                                                                  aGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCtt                                   <  1:3326829/100‑1 (MQ=255)
                                                                  aGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCtt                                   <  1:2480005/100‑1 (MQ=255)
                                                                   gTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTa                                  <  1:475786/100‑1 (MQ=255)
                                                                   gTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCAATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTa                                  >  2:1915740/1‑100 (MQ=255)
                                                                       aGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGctc                              >  2:404067/1‑100 (MQ=255)
                                                                          gTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGctctag                           >  1:2129205/1‑98 (MQ=255)
                                                                            tCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGaa                         >  1:886565/1‑100 (MQ=255)
                                                                             cAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAg                        >  1:1076673/1‑100 (MQ=255)
                                                                             cAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAg                        >  1:3454097/1‑100 (MQ=255)
                                                                               gAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAggg                      <  1:3003107/100‑1 (MQ=255)
                                                                               gAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAggg                      <  1:4133999/100‑1 (MQ=255)
                                                                                ataTAAGAAACCCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAgggg                     <  2:3467633/98‑1 (MQ=255)
                                                                                    aaGAAAACCCTTAAGTCTGTGCGACACAGTCTTAAGGGTTTCTACCCCTTCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGAt                 <  2:3132759/100‑1 (MQ=255)
                                                                                     aGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATa                >  2:492801/1‑100 (MQ=255)
                                                                                       aaaaCCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAg              <  2:2884386/100‑1 (MQ=255)
                                                                                        aaaCCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGa             >  2:514390/1‑100 (MQ=255)
                                                                                        aaaCCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGa             >  2:3874295/1‑100 (MQ=255)
                                                                                        aaaCCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCACAGTGGCTTAGCTCTTGAAGGGGCGATAAGa             >  1:1713101/1‑100 (MQ=255)
                                                                                          aCCCTTAAGCCTGTGTCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaat           >  2:2336212/1‑100 (MQ=37)
                                                                                           cccTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGa             <  2:2620326/97‑1 (MQ=255)
                                                                                            ccTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaataa         >  1:338678/1‑100 (MQ=255)
                                                                                            ccTTAAGCCTGTGTCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaataa         >  2:2517015/1‑100 (MQ=37)
                                                                                             cTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaataat        <  1:2266339/100‑1 (MQ=255)
                                                                                              ttAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAAtc       <  1:4015204/100‑1 (MQ=255)
                                                                                              ttAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAAtc       <  2:130410/100‑1 (MQ=255)
                                                                                              ttAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAAtc       <  1:2239054/100‑1 (MQ=255)
                                                                                                aaGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAAtctc     <  2:696521/100‑1 (MQ=255)
                                                                                                 aGTTTGAGCGACACAGGCTTGAGAGTTTCTATCCCATCCCTCTCTTATCTACCGCACTCTCAGTGGCTTAGCTCTTGACGGGGCGATAAGAATAAtc       <  2:3165809/97‑1 (MQ=255)
                                                                                                 aGTCTGTGCGACTCAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTTAGTGGCTTAGCTCTTGAAGGTGCGATAAGAATAATCTCa    >  2:1106332/1‑100 (MQ=255)
                                                                                                 aGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCa    >  1:917091/1‑100 (MQ=255)
                                                                                                 aGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCa    <  1:1749807/100‑1 (MQ=255)
                                                                                                  gTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCAt   >  1:3971331/1‑100 (MQ=255)
                                                                                                   tCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATa  <  1:295171/100‑1 (MQ=255)
                                                                                                   |                                                                                                    
GCATTAAGGCTGTGATGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATA  >  NC_000913/1764588‑1764786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: