Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,778,470 A→G 56.5% Y278H (TAC→CAC)  ydiP ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,778,4700AG56.5% 52.7 / 108.2 100Y278H (TAC→CAC) ydiPputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (21/22);  new base G (27/29);  total (49/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

ACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGCCTGTGATAATT  >  NC_000913/1778371‑1778568
                                                                                                   |                                                                                                  
aCAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTa                                                                                                    >  1:361419/1‑100 (MQ=255)
aCAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTa                                                                                                    >  1:339061/1‑100 (MQ=255)
   aaCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATc                                                                                                 <  1:1716051/100‑1 (MQ=255)
     cTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGc                                                                                               >  1:1957750/1‑100 (MQ=255)
       gcgcCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTg                                                                                             <  1:3555098/100‑1 (MQ=255)
        cgccgcCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTgg                                                                                            >  1:1937710/1‑100 (MQ=255)
        cgccgcCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTgg                                                                                            <  1:3778835/100‑1 (MQ=255)
         gccgccAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGTAAAACAGTTTTTAAATTGTCTACGGTGATCGCTggg                                                                                           <  1:2909418/100‑1 (MQ=255)
         gccgccAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTAAAATTGTCTACGGTAATCGCTggg                                                                                           >  2:862743/1‑100 (MQ=255)
          ccgccAGATTTATGCAACAAGACTTACCGGTCGAGGAAATTCGGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGc                                                                                          >  1:2792511/1‑100 (MQ=255)
          ccgccAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGc                                                                                          <  1:3362162/100‑1 (MQ=255)
          ccgccAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGc                                                                                          >  2:3629694/1‑100 (MQ=255)
            gccAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGa                                                                                        <  1:1916252/100‑1 (MQ=255)
             ccAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGaa                                                                                       >  1:1898211/1‑100 (MQ=255)
              cAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGaac                                                                                      >  1:1378752/1‑100 (MQ=255)
                 aTTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGaacaac                                                                                   <  1:3607038/100‑1 (MQ=255)
                   ttATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCg                                                                                 <  1:1610415/100‑1 (MQ=255)
                    ccgatctcaaGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGa                                                                                <  2:3210360/93‑1 (MQ=255)
                     aTCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGAc                                                                               <  1:1038687/100‑1 (MQ=255)
                     aTCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGAc                                                                               >  1:2333393/1‑100 (MQ=255)
                     aTCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGAc                                                                               >  2:576211/1‑100 (MQ=255)
                         aacaaGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATgg                                                                           >  2:2592398/1‑100 (MQ=255)
                         aacaaGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATgg                                                                           >  1:2032946/1‑100 (MQ=255)
                          acgaGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgc                                                                          <  1:3258682/97‑1 (MQ=255)
                          acaaGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGATAATCGCTGGGCGAACAACCGACATGgc                                                                          <  2:4026095/100‑1 (MQ=255)
                           caaGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGaagatcgg                                                                         >  1:3210380/1‑93 (MQ=255)
                            aaGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGgcgc                                                                        <  1:1167498/100‑1 (MQ=255)
                            aaGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgc                                                                          >  2:1925189/1‑98 (MQ=255)
                                cTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCaaaa                                                                    >  2:741132/1‑100 (MQ=255)
                                 ttACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCaaa                                                                     <  2:110550/98‑1 (MQ=255)
                                  tACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAAc                                                                  >  1:4027161/1‑100 (MQ=255)
                                    ccGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAAc                                                                  <  2:1563067/98‑1 (MQ=255)
                                      ggctGAGGAAATTCCGATAGGATTTCTTGTTCCGCAAAACAGATTTTAAATTGTCTACGGTCATCGCTGCGCGAACAACCGACATGGCGCAAAAACAGtt                                                              >  2:3350694/4‑100 (MQ=255)
                                      ggTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGtt                                                              >  1:2514077/1‑100 (MQ=255)
                                         tGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTgg                                                           <  2:1378743/100‑1 (MQ=255)
                                         tGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTgg                                                           <  1:624005/100‑1 (MQ=255)
                                           aGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCa                                                         >  2:2834605/1‑100 (MQ=255)
                                            ggAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCaa                                                        >  2:1935303/1‑100 (MQ=255)
                                            ggAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCaa                                                        >  1:1025360/1‑100 (MQ=255)
                                            ggAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGATTTTAAATTGTCTACGTTGAACGATGCGCAAACAACAGACATGGCGCAAAAACAGTTTGGCa                                                         >  2:2281002/1‑99 (MQ=255)
                                            ggAAATTCAGAGAGGATTTCTTGTTCCGCAAAACAGATCTTAAATTGACTAGGGTGATCGCTGGCCGTACAACAAACATGCCGCAAAAACactgaggca                                                         >  2:2274022/1‑91 (MQ=255)
                                             gAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCaaa                                                       >  1:16773/1‑100 (MQ=255)
                                               aaTTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGt                                                     <  2:114940/100‑1 (MQ=255)
                                                    aGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCg                                                <  1:3402198/100‑1 (MQ=255)
                                                    aGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCg                                                <  1:2156899/100‑1 (MQ=255)
                                                      aTAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGAc                                              >  1:3715744/1‑100 (MQ=255)
                                                      aTAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGAc                                              >  2:2618098/1‑100 (MQ=255)
                                                       tAGGATTTGTTGTACGGCAAAACAGGTTTTAAATTATCTACGGTAATCGCTGGGCGAACAACCGAAATAACAAAAAAACAGTTTGGCAAAGTGATCGAc                                              <  2:1441190/99‑1 (MQ=255)
                                                       tAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACa                                             >  1:251542/1‑100 (MQ=255)
                                                       tAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACa                                             >  1:1291555/1‑100 (MQ=255)
                                                          gATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACAttt                                          >  2:2119158/1‑100 (MQ=255)
                                                           aTTTCTTGTTCCGGAAAACAGTTTTTAAATTGTATACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACagt                                           >  2:2555842/1‑96 (MQ=255)
                                                           aTTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACAtttt                                         >  1:1947301/1‑100 (MQ=255)
                                                           aTTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACAtttt                                         <  1:3178776/100‑1 (MQ=255)
                                                            tgtCTTGCTACGCAAAACAGATCTTAAATTGCCTACTGTGATCGTTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTc                                        <  2:2619156/98‑1 (MQ=255)
                                                             ttCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCa                                       <  1:2474499/100‑1 (MQ=255)
                                                              tCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCa                                       <  2:3010156/99‑1 (MQ=255)
                                                              tCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCAt                                      >  1:1995634/1‑100 (MQ=255)
                                                              tCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCAt                                      >  1:2427540/1‑100 (MQ=255)
                                                              tCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCAt                                      >  2:2912091/1‑100 (MQ=255)
                                                               cTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCAt                                      <  2:3255314/99‑1 (MQ=255)
                                                                 tGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAAc                                   >  2:640306/1‑100 (MQ=255)
                                                                 tGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAAc                                   <  2:2083649/100‑1 (MQ=255)
                                                                 tGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAAc                                   >  1:3965030/1‑100 (MQ=255)
                                                                 tGTACCACAAAACAGTTTATACATTGTCTCCGGTAATCGCTGAGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATaa                                    <  1:608773/99‑1 (MQ=255)
                                                                  gTTCCGCAAAACAGTTTTTAAATTTTTTATGGTAATCGCTAGGCGAACAACCAACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAAcc                                  >  2:769642/1‑100 (MQ=255)
                                                                  gTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAAcc                                  <  1:2399735/100‑1 (MQ=255)
                                                                  gTTCCGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAAcc                                  <  2:3894263/100‑1 (MQ=255)
                                                                  gTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAAcc                                  >  1:2727978/1‑100 (MQ=255)
                                                                  gTTCCGCAAAACAGTTTTTAAATTGTCGACGGTAAGCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATa                                     <  2:1359745/97‑1 (MQ=255)
                                                                   ttCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAAccc                                 >  1:336724/1‑100 (MQ=255)
                                                                     ccGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCcac                               <  1:103423/100‑1 (MQ=255)
                                                                      cGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCcaca                              >  1:1493186/1‑100 (MQ=255)
                                                                      cGCAAAACAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCcaca                              <  2:1038353/100‑1 (MQ=255)
                                                                          aaaCAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGcc                          <  1:214869/100‑1 (MQ=255)
                                                                            aCAGTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGTTGTGGCGCAACAACAGTTTGGCAAAGTGATCGACGTTTTCATAACCCACACGCCAg                        >  2:578271/1‑100 (MQ=255)
                                                                            aCAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATTGCGCAAAAACAGTTTGGCAAAGTCAGCGACATTTTCATAACACACACGCCAg                        >  2:2863146/1‑100 (MQ=255)
                                                                               gTTTTTAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGgag                     <  1:2155214/100‑1 (MQ=255)
                                                                                tttttAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGgaga                    <  1:992868/100‑1 (MQ=255)
                                                                                tttttAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGgaga                    >  1:3549661/1‑100 (MQ=255)
                                                                                 ttttAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGAt                   >  1:1792787/1‑100 (MQ=255)
                                                                                    tAAATTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGAtctc                >  1:2834366/1‑100 (MQ=255)
                                                                                     aaaTTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATctct               >  1:2106554/1‑100 (MQ=255)
                                                                                     aaaTTGTCTACGGTAATCGCTGGGCGAACAAAAAAAAAAAAAAAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATctct               <  2:32728/100‑1 (MQ=255)
                                                                                      aaTTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAACACAGTTTGGCACAGTGCTCAACATTTTCATAACCCACACGCCAGGAGAtc                  >  2:3535356/1‑96 (MQ=255)
                                                                                      aaTTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTg              <  2:3244027/100‑1 (MQ=255)
                                                                                       aTTGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGc             <  1:3308069/100‑1 (MQ=255)
                                                                                       aTTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGc             <  2:1850155/100‑1 (MQ=255)
                                                                                        ttGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGcc            <  1:560122/100‑1 (MQ=255)
                                                                                        ttGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGcc            <  1:1506291/100‑1 (MQ=255)
                                                                                        ttGTCTACGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTAGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGcc            <  2:2817925/100‑1 (MQ=255)
                                                                                          gTCTACGGTGATCGCGGGGTGAACAACCGACATGGCGCAAAAACAGCTTGGCAAAGTGATCGACATTTTCATAACCCACATGCCAGGAGATCTCTGCCt           >  2:2700150/1‑99 (MQ=255)
                                                                                          gTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAATCTGTTTGTTAAAGATACCGCGACTATCATAACCCACACGCCAGGAGATCTCTGCCtg          <  2:354589/100‑1 (MQ=255)
                                                                                            cTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGCCtgtg        <  1:3016951/100‑1 (MQ=255)
                                                                                              aCGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGCCTGTGAt      >  2:1366397/1‑100 (MQ=255)
                                                                                              aCGGTAATCGCTGGGCTAACAACCTACATGGCCCAAAAACAGTTTGGCAAAGTGATTGACACTTTCATAACCCACACGCCAGGAGATCTCTGCCTGTGAt      <  2:4058208/100‑1 (MQ=255)
                                                                                              aCGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGCCTGTGAt      <  1:974815/100‑1 (MQ=255)
                                                                                               cGGTGATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGCCTGTGATa     >  2:539168/1‑100 (MQ=255)
                                                                                               cGGTAAGCGCTTGGCGAACGACCGACATGGCGCAACAACAGTTTGGCAAAGTGATCGTCATTGTCATAACCCACTCGCCAGGAGATCTTTGCCTGTGAt      <  2:1955893/99‑1 (MQ=255)
                                                                                                  tAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGCCTGTGATAAtt  <  2:339058/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
ACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCGACATTTTCATAACCCACACGCCAGGAGATCTCTGCCTGTGATAATT  >  NC_000913/1778371‑1778568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: