Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,827,031 T→A 100% D410V (GAT→GTT)  astB ← succinylarginine dihydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,827,0310TA98.2% 356.0 / ‑3.1 110D410V (GAT→GTT) astBsuccinylarginine dihydrolase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (65/43);  total (66/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 110 total aligned reads displayed.

CTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCC  >  NC_000913/1826934‑1827126
                                                                                                 |                                                                                               
ctcGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACg                                                                                               <  1:3902872/100‑1 (MQ=255)
  cgcgCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgc                                                                                             <  2:2931252/100‑1 (MQ=255)
    cttccgatctgATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcag                                                                                           <  1:960174/90‑1 (MQ=255)
     gcTGGACCGGATAAACCGAACCGCGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcagc                                                                                          <  1:3293569/100‑1 (MQ=255)
      cTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCCCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcacca                                                                                         >  2:1263008/1‑100 (MQ=255)
       tGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGcagcag                                                                                        <  2:3827839/100‑1 (MQ=255)
         gAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTa                                                                                      <  1:3310011/100‑1 (MQ=255)
          aaCGGATAAACCGACCCGAGATTCATTACTTGCCCCCATACATCCACTGCTTCCCGCCTTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCCGCAGTaa                                                                                     >  2:332474/1‑100 (MQ=255)
          aaCGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTaa                                                                                     >  2:4004905/1‑100 (MQ=255)
           aCGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAg                                                                                    <  1:1997126/100‑1 (MQ=255)
            cGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAgg                                                                                   <  2:2989807/100‑1 (MQ=255)
             ggATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGc                                                                                  >  1:3544963/1‑100 (MQ=255)
             ggATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGc                                                                                  >  1:1394984/1‑100 (MQ=255)
              gATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCg                                                                                 >  1:3304832/1‑100 (MQ=255)
              gATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCg                                                                                 >  1:3850082/1‑100 (MQ=255)
              gATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTAACGCAGAAATTGCGGGGCGGCCAGCACGGCCGCAGTAAGGCg                                                                                 >  2:301986/1‑100 (MQ=255)
                tAAACCGAACCTAGATTCAGTAATTGGCGCATTACCTCCAGTTCTGCCCGCCGTTCGCGCAGCAATTGGGGGTCGGCCAGAACGGAAGCAGTAAGGCTAt                                                                               >  2:1847427/1‑100 (MQ=255)
                tAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGAt                                                                               <  1:1496007/100‑1 (MQ=255)
                tAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGAGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGc                                                                                  >  2:907570/1‑97 (MQ=255)
                  naccgaaccgaGATTCAGTAATTGGCTCAATAAACACAGTACTTCCCGCCCTTCGCGCAGCAATTACGGGCCGGCCAGGACGGCAGAAGGAAGCCGAGcg                                                                             >  2:2022630/2‑100 (MQ=255)
                   accgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcgc                                                                            <  2:356792/100‑1 (MQ=255)
                   accgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGAAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGACcg                                                                             >  1:1379648/1‑99 (MQ=255)
                    ccgaaccgaGATTCAGTAATTGGCTCAATCCTTCCAGAGCTTCCGGCCCTTCGCGCAGCAATTGGGGGTCGGCCCGAACGGCTGCAGTACGCCGATcgc                                                                            >  2:261267/1‑99 (MQ=255)
                    ccgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcgcg                                                                           >  1:2711543/1‑100 (MQ=255)
                     cgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCgg                                                                          <  1:1083531/100‑1 (MQ=255)
                      gaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgt                                                                         <  2:3993670/100‑1 (MQ=255)
                      gaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgt                                                                         <  1:2087759/100‑1 (MQ=255)
                        accgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGgtag                                                                       <  2:2292187/100‑1 (MQ=255)
                         ccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtagt                                                                      >  1:604521/1‑100 (MQ=255)
                         ccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgcag                                                                       >  2:2667318/1‑99 (MQ=255)
                          cgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGgtagta                                                                     >  2:2126134/1‑100 (MQ=255)
                          cgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtagta                                                                     <  2:3662983/100‑1 (MQ=255)
                            agaTTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAAc                                                                   <  1:2227602/100‑1 (MQ=255)
                             gaTTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACg                                                                  <  1:3537859/100‑1 (MQ=255)
                               ttCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGAt                                                                >  1:1992075/1‑100 (MQ=255)
                                 cAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATcc                                                              >  2:86267/1‑100 (MQ=255)
                                    tAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAcc                                                           >  1:2641005/1‑100 (MQ=255)
                                     aaTTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGTTAGTAACGATCCAccc                                                          <  2:2869798/100‑1 (MQ=255)
                                     aaTTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAccc                                                          >  2:4127949/1‑100 (MQ=255)
                                     aaTTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAccc                                                          <  1:3162918/100‑1 (MQ=255)
                                      aTTGGCTCAATACATCCAGTGCTTCCGGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAACGCAATCGCGGAAGTAAAGATCCACCCa                                                         >  1:2508682/1‑100 (MQ=255)
                                        tGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGt                                                       >  1:3291627/1‑100 (MQ=255)
                                        tGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGt                                                       >  1:4048632/1‑100 (MQ=255)
                                         ggCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTc                                                      >  1:616933/1‑100 (MQ=255)
                                           cTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCAt                                                    >  1:3100782/1‑100 (MQ=255)
                                            tCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCAtt                                                   >  1:1143050/1‑100 (MQ=255)
                                             cAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTg                                                  >  2:1403137/1‑100 (MQ=255)
                                             cAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTg                                                  >  1:265943/1‑100 (MQ=255)
                                              aaTACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGa                                                 >  2:364952/1‑100 (MQ=255)
                                              aaTACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGa                                                 >  1:2541391/1‑100 (MQ=255)
                                              aaTACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGa                                                 <  1:4006867/100‑1 (MQ=255)
                                                tACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTACGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgc                                               >  2:1949190/1‑100 (MQ=255)
                                                tACATCCAGTGCTTCCCACCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgc                                               <  2:2144693/100‑1 (MQ=255)
                                                 aCATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcg                                              <  2:3743845/100‑1 (MQ=255)
                                                 aCATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcg                                              <  1:3084808/100‑1 (MQ=255)
                                                  cATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcgc                                             >  1:1996476/1‑100 (MQ=255)
                                                   aTCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCa                                            >  2:1025738/1‑100 (MQ=255)
                                                    tCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAt                                           >  1:3415900/1‑100 (MQ=255)
                                                    tCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGAGCAt                                           >  1:3727544/1‑100 (MQ=255)
                                                     ccAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCCGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAtt                                          >  2:602989/1‑100 (MQ=255)
                                                     ccAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAtt                                          >  1:290836/1‑100 (MQ=255)
                                                     ccAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAtt                                          <  1:1089137/100‑1 (MQ=255)
                                                     ccAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAtt                                          <  2:3752900/100‑1 (MQ=255)
                                                      cAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTa                                         >  2:1234042/1‑100 (MQ=255)
                                                      cAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTa                                         >  2:1641089/1‑100 (MQ=255)
                                                      cAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTa                                         >  1:3623167/1‑100 (MQ=255)
                                                       aGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTaa                                        >  1:1022536/1‑100 (MQ=255)
                                                       aGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTaa                                        >  1:288495/1‑100 (MQ=255)
                                                         tGCTTCCCGCCCGTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAAc                                      <  1:1208350/100‑1 (MQ=255)
                                                          gCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGAGATCGCGGGAGTACCGATCCACCCAGTCATTGAGCGCATTAAACa                                     >  2:638613/1‑100 (MQ=255)
                                                           cTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAg                                    <  1:476051/100‑1 (MQ=255)
                                                           cTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAg                                    <  1:3621897/100‑1 (MQ=255)
                                                           cTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAACGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCCCATTAAACa                                     >  1:2847938/1‑99 (MQ=255)
                                                            ttCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGACCCACCCAGTCATTGAGCGCATTCAACAGc                                   >  2:2563019/1‑100 (MQ=255)
                                                             tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg                                  >  1:1922317/1‑100 (MQ=255)
                                                              cccGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg                                  <  2:1124524/99‑1 (MQ=255)
                                                                cGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTAt                               <  1:516096/100‑1 (MQ=255)
                                                                cGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTAt                               <  1:3389349/100‑1 (MQ=255)
                                                                cGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTAt                               <  2:3661621/100‑1 (MQ=255)
                                                                cGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTAt                               <  2:3737432/100‑1 (MQ=255)
                                                                   ccTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGt                            >  2:863666/1‑100 (MQ=255)
                                                                    cTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGtt                           >  1:1904784/1‑100 (MQ=255)
                                                                    cTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGtt                           <  1:1438781/100‑1 (MQ=255)
                                                                      tCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtca                         <  2:1691661/100‑1 (MQ=255)
                                                                       cgcgCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcat                        >  2:3277589/1‑100 (MQ=255)
                                                                         cgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatca                      <  1:2378484/100‑1 (MQ=255)
                                                                         cgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatca                      <  2:529289/100‑1 (MQ=255)
                                                                         cgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatca                      >  1:1110422/1‑100 (MQ=255)
                                                                           cagcaATTGCGGGTCGGCCAGAACGGCAGCCGTAAGGCGATCGCGGTAGTAACGATCCACCCAGGCATTGCGCGCATTAAACAGCGCATCGTtcatca                      >  2:800080/1‑98 (MQ=255)
                                                                             gcaATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAc                  <  2:1022530/100‑1 (MQ=255)
                                                                                  tGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgccg             <  2:528214/100‑1 (MQ=255)
                                                                                     gggTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGAt          <  1:170660/100‑1 (MQ=255)
                                                                                     gggTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGAt          <  2:1550179/100‑1 (MQ=255)
                                                                                      ggTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGAtt         >  1:2505641/1‑100 (MQ=255)
                                                                                       gTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTc        >  2:1689714/1‑100 (MQ=255)
                                                                                       gTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTc        <  1:740480/100‑1 (MQ=255)
                                                                                         cGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCAc      <  1:320517/100‑1 (MQ=255)
                                                                                          ggCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCAcc     >  1:2426135/1‑100 (MQ=255)
                                                                                           gCCAGAACGGCAGCAGTAAGGCGATCGCGTTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCAcc     >  2:762724/1‑99 (MQ=255)
                                                                                           gCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCg    >  1:2836610/1‑100 (MQ=255)
                                                                                                 |                                                                                               
CTCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCC  >  NC_000913/1826934‑1827126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: