Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,865,682 G→C 15.5% intergenic (‑46/+44) yeaE ← / ← mipA aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,865,6820GC15.5% 368.2 / 59.7 143intergenic (‑46/+44)yeaE/mipAaldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery
Reads supporting (aligned to +/- strand):  ref base G (49/71);  new base C (12/10);  total (62/81)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-01

Only 100 of 145 total aligned reads displayed.

CCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCA  >  NC_000913/1865584‑1865781
                                                                                                  |                                                                                                   
ccTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGa                                                                                                    <  1:439322/100‑1 (MQ=255)
 cTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGAc                                                                                                   >  2:2094813/1‑100 (MQ=255)
  tACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTTCATTGGATCCTCCCCGGTATTGCACCACCGTAATGCAAGACAGGGCGTTACg                                                                                                  >  2:3090816/1‑100 (MQ=255)
   aCGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGc                                                                                                 <  1:1454302/100‑1 (MQ=255)
   aCGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATCTTTTGCTGCATTGTTGCCTCCCAGGTATTGCACCACCGAACTGCAACACAGGGCGTGACGc                                                                                                 <  2:3066305/100‑1 (MQ=255)
    cGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGcc                                                                                                >  1:2880775/1‑100 (MQ=255)
    cGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGcc                                                                                                >  2:1921375/1‑100 (MQ=255)
       cTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTg                                                                                             >  2:731178/1‑100 (MQ=255)
         ggCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGtt                                                                                           <  1:3358530/100‑1 (MQ=255)
          gCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGttt                                                                                          >  2:2857365/1‑100 (MQ=255)
           cAGTGAGACATCGCCACTAAATTGAATCATTTTTTTTTTTTTTTTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGtttt                                                                                         >  2:1062709/1‑100 (MQ=255)
           cAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtttt                                                                                         <  1:1166724/100‑1 (MQ=255)
           cAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGAATCGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtttt                                                                                         <  2:2908647/100‑1 (MQ=255)
           cAGTGAGACAGCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCATAATGCAAAACAGGGCGTGACGCCCTGtttt                                                                                         <  2:1606321/100‑1 (MQ=255)
            actGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttg                                                                                        <  1:2128668/98‑2 (MQ=255)
            aGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttg                                                                                        <  1:693220/100‑2 (MQ=255)
             gTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttgc                                                                                       <  1:694693/100‑3 (MQ=255)
             gTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTGTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttgc                                                                                       <  1:1927452/100‑3 (MQ=255)
              tGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTAt                                                                                      <  1:232474/100‑1 (MQ=255)
              tGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttgca                                                                                      <  1:1243818/100‑4 (MQ=255)
               gagaCATCGCCACTAAATTTAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATg                                                                                     <  2:1321576/100‑1 (MQ=255)
               gagaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATg                                                                                     >  1:3113366/1‑100 (MQ=255)
               gagaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATg                                                                                     <  1:3079607/100‑1 (MQ=255)
                agaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGc                                                                                    >  2:50357/1‑100 (MQ=255)
                agaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGc                                                                                    <  1:1420277/100‑1 (MQ=255)
                 gaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATg                                                                                     <  2:2374958/98‑1 (MQ=255)
                 gaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGca                                                                                   <  1:3614525/100‑1 (MQ=255)
                  aCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGca                                                                                   <  2:3328882/99‑1 (MQ=255)
                   cATCGCCACTAGATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGcaca                                                                                 >  2:4127343/1‑100 (MQ=255)
                   cATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGcaca                                                                                 >  1:2227525/1‑100 (MQ=255)
                    aTCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTGCTGCCAGGTATTGCACAAGCGTAATGCAAATCAGGGCGTGACGCCCTGTTTTTATGCACaa                                                                                >  2:2531733/1‑100 (MQ=255)
                     tCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaaa                                                                               <  1:255683/100‑1 (MQ=255)
                       gCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGTACAAAAt                                                                             >  2:1709473/1‑100 (MQ=255)
                        ccACTAAATTGAATCATTTTTTTTTGCATTGTTTCCTCCCAGGTATTGCACCACCTTAATGCAAAATAGGGCGTGTCGCCCTGTTTTTATGCACAAAGTg                                                                            >  2:1575600/1‑100 (MQ=255)
                             aaaTTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACCGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTg                                                                       <  2:2202500/100‑1 (MQ=255)
                              aactGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTgg                                                                      <  1:1579104/97‑1 (MQ=255)
                              aaTTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTgg                                                                      <  1:1555082/100‑1 (MQ=255)
                                 tGAATCATTTTTTATTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGaaa                                                                   <  1:665672/100‑1 (MQ=255)
                                    aTCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGAt                                                                >  1:2286748/1‑100 (MQ=255)
                                     tCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATg                                                               <  1:284219/100‑1 (MQ=255)
                                        ttttttGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCAt                                                            <  2:1164971/100‑1 (MQ=255)
                                            ttgttgCATTGTTTCCTCCCTGGTATTTCACCATCGTAATGCAAAACAGGGCGTGACGCTCTGTTTTTATGCACTAAATGCGCTGGAAAGATGCATTATc                                                        <  2:1901599/100‑1 (MQ=255)
                                            ttgttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATc                                                        <  2:1779706/100‑1 (MQ=255)
                                             tgttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCa                                                       <  1:3015350/100‑1 (MQ=255)
                                              gttgCATTGTTGCCTCCCAGGTATCGCATCACCCTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAg                                                      <  2:2576763/100‑1 (MQ=255)
                                                 gCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAt                                                   <  2:3026340/100‑1 (MQ=255)
                                                 gCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAt                                                   >  2:649615/1‑100 (MQ=255)
                                                  cATTGTTTCCTCGCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAtt                                                  >  1:2724718/1‑100 (MQ=255)
                                                  cATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAtt                                                  <  2:3598625/100‑1 (MQ=255)
                                                    ttGTTTCCTCCCAGGTCTTCCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTg                                                <  2:3889530/100‑1 (MQ=255)
                                                     tGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTg                                                <  1:633449/99‑1 (MQ=255)
                                                     tGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGt                                               <  1:3826561/100‑1 (MQ=255)
                                                      gTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTa                                              <  1:1508589/100‑1 (MQ=255)
                                                        ttCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAgg                                            <  1:1907423/100‑1 (MQ=255)
                                                        ttCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAgg                                            >  2:3770499/1‑100 (MQ=255)
                                                         tcctccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGt                                           >  2:1034703/1‑100 (MQ=255)
                                                          cctccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTg                                          >  2:246053/1‑100 (MQ=255)
                                                            tccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAt                                        >  2:3558574/1‑100 (MQ=255)
                                                              ccAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATcc                                      >  2:814135/1‑100 (MQ=255)
                                                                 ggTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCgg                                   >  2:897468/1‑100 (MQ=255)
                                                                  gTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGt                                  >  1:3110066/1‑100 (MQ=255)
                                                                  gTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGt                                  <  1:3299081/100‑1 (MQ=255)
                                                                   tATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTa                                 <  1:2132915/100‑1 (MQ=255)
                                                                     ttGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGa                               <  1:2929845/100‑1 (MQ=255)
                                                                        caccacCGTAATGCAAAACAGGGCGTGACGCCCTGTGTTTATGCACAAAATGCCGTGGAAAGATGCATTATCAGCATTTGTCGGTGATCCCGGTAGa                               >  1:1122527/1‑97 (MQ=255)
                                                                          ccaccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGAGGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCa                          >  2:3966441/1‑100 (MQ=255)
                                                                           caccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTTATCCCGGTAGAAATCAg                         <  1:249939/100‑1 (MQ=255)
                                                                           caccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAg                         <  1:3558710/100‑1 (MQ=255)
                                                                           caccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAg                         <  1:1498622/100‑1 (MQ=255)
                                                                             ccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                       <  2:1573412/100‑1 (MQ=255)
                                                                             ccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTAAGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                       >  1:2913155/1‑100 (MQ=255)
                                                                                cttccgatctcAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAg                    <  1:2485616/90‑1 (MQ=255)
                                                                                 gcgtggaagtaGGGCGTCAGGTCGTGTGTTTATGGACAAAATGCCCTGGAAAGATGCATTATCAGAAGTTGTAGGTAATCCAGGTAGAAATCAGGGCAGt                   >  2:3926701/11‑100 (MQ=255)
                                                                                 gcataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGt                   >  1:2955959/6‑100 (MQ=255)
                                                                                 gcataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGt                   >  2:3954263/6‑100 (MQ=255)
                                                                                 gcataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGt                   >  1:74644/6‑100 (MQ=255)
                                                                                  cataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTc                  >  1:1070451/5‑100 (MQ=255)
                                                                                  cataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTc                  >  1:86475/5‑100 (MQ=255)
                                                                                  aTGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTc                  >  1:3493683/1‑100 (MQ=255)
                                                                                   tGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTcc                 <  2:657492/100‑1 (MQ=255)
                                                                                    taaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCa                >  2:964964/3‑100 (MQ=255)
                                                                                    gCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCa                >  1:4037348/1‑100 (MQ=255)
                                                                                     cAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAg               <  2:3113347/100‑1 (MQ=255)
                                                                                     cAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAg               >  2:2568053/1‑100 (MQ=255)
                                                                                     aaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAg               >  1:3474217/2‑100 (MQ=255)
                                                                                      aaaaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAgg              <  2:3318194/100‑1 (MQ=255)
                                                                                      aaaaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAgg              <  1:1065752/100‑1 (MQ=255)
                                                                                      aaaaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAgg              <  1:3552516/100‑1 (MQ=255)
                                                                                       aaaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGa             >  1:3098412/1‑100 (MQ=255)
                                                                                        aaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAt            <  2:2531365/100‑1 (MQ=255)
                                                                                        aaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAt            <  2:1127804/100‑1 (MQ=255)
                                                                                        aaCAGGGCGTGACGCCCTGTTTTTATGCAAAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAAAAATCAGGCCAGTCCAGGAt            <  1:3770523/100‑1 (MQ=255)
                                                                                        aaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAt            >  1:2976402/1‑100 (MQ=255)
                                                                                         aCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAtt           >  1:1494851/1‑100 (MQ=255)
                                                                                          cAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAgagatcggaag          >  2:2485602/1‑90 (MQ=255)
                                                                                          cAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAttt          >  1:4014819/1‑100 (MQ=255)
                                                                                            gggCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTAt        >  2:3206855/1‑100 (MQ=255)
                                                                                                gTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATccac    <  1:1019656/100‑1 (MQ=255)
                                                                                                 tGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATccacc   <  1:1120833/100‑1 (MQ=255)
                                                                                                 tGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATccacc   <  2:1195084/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
CCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCA  >  NC_000913/1865584‑1865781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: