Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,965,088 C→T 15.5% V368V (GTG→GTA) ‡ flhB ← flagellin export apparatus, substrate specificity protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,965,0880CT15.5% 318.6 / 40.9 129V368V (GTG→GTAflhBflagellin export apparatus, substrate specificity protein
Reads supporting (aligned to +/- strand):  ref base C (64/45);  new base T (13/7);  total (77/52)
Fisher's exact test for biased strand distribution p-value = 8.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

Only 100 of 134 total aligned reads displayed.

ATCTGCCATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTAC  >  NC_000913/1964989‑1965187
                                                                                                   |                                                                                                   
aTCTGCCATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGc                                                                                                     <  1:1793923/100‑1 (MQ=255)
  cTGCCATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGc                                                                                                     >  2:793804/1‑98 (MQ=255)
    gCCATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCAcc                                                                                                  >  2:1244470/1‑99 (MQ=255)
    gCCATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCg                                                                                                 >  1:2351805/1‑100 (MQ=255)
      cATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGa                                                                                               >  1:3961865/1‑100 (MQ=255)
        ttGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAg                                                                                             <  2:2974800/100‑1 (MQ=255)
          ntgtCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATACAATCCAGGGCTTCCGGCACCGGAAGAt                                                                                           >  2:2988479/2‑100 (MQ=255)
           tgtCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATg                                                                                          <  1:3384818/100‑1 (MQ=255)
           tgtCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATg                                                                                          <  1:4135283/100‑1 (MQ=255)
           tgtCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATg                                                                                          <  1:1343881/100‑1 (MQ=255)
           tgtCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATg                                                                                          <  2:612638/100‑1 (MQ=255)
           tgtCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATg                                                                                          <  1:1104598/100‑1 (MQ=255)
            gtCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccct                                                                                         <  1:1142347/100‑5 (MQ=255)
            gtCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccct                                                                                         <  1:3931930/100‑5 (MQ=255)
            gtCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccct                                                                                         <  1:3566745/100‑5 (MQ=255)
            gtCGATTTCAGGTTTGCGGGCAGGAGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATg                                                                                          >  2:206564/1‑99 (MQ=255)
             tCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCTGGAAgccctg                                                                                        <  2:2228356/100‑6 (MQ=255)
             tCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccctg                                                                                        <  2:2621180/100‑6 (MQ=255)
             tCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccctg                                                                                        <  1:3730666/100‑6 (MQ=255)
              cGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGt                                                                                       >  2:1104303/1‑100 (MQ=255)
              cGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGt                                                                                       >  1:797343/1‑100 (MQ=255)
                  ttCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGt                                                                                   <  2:1108609/100‑1 (MQ=255)
                    cAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTg                                                                                 <  1:536682/100‑1 (MQ=255)
                     aGGTTTGCGGGCAGGCGCAGCAGCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTg                                                                                 <  2:786681/99‑1 (MQ=255)
                      ggTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTa                                                                               >  1:438021/1‑100 (MQ=255)
                            cGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGgc                                                                         >  1:3705992/1‑100 (MQ=255)
                            cGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATACAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGgc                                                                         >  2:2163352/1‑100 (MQ=255)
                               gCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCCCCGGTAGATGAGTAGATTGTACAGGGCGCt                                                                      >  2:954133/1‑100 (MQ=255)
                                cAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTg                                                                     <  1:2334764/100‑1 (MQ=255)
                                  ggCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTc                                                                   >  1:2749681/1‑100 (MQ=255)
                                  ggCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTc                                                                   <  1:471555/100‑1 (MQ=255)
                                  ggCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTc                                                                   >  1:3990166/1‑100 (MQ=255)
                                   gcgcAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTcc                                                                  <  2:545536/100‑1 (MQ=255)
                                   gcgcAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTcc                                                                  >  2:4026936/1‑100 (MQ=255)
                                    cgcAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTcca                                                                 <  1:2134455/100‑1 (MQ=255)
                                     gcagcaTCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTccac                                                                <  1:734603/100‑1 (MQ=255)
                                     gcagcaTCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTccac                                                                >  1:3572510/1‑100 (MQ=255)
                                        gcaTCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCAccag                                                             >  1:2963533/1‑100 (MQ=255)
                                          aTCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCGGGGCTTCCGGCACCGGAAGATGAGTATGTTGTACAGGGCGCTGGCCAccagcc                                                           <  2:4069244/100‑1 (MQ=255)
                                          aTCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCAccagcc                                                           <  1:702179/100‑1 (MQ=255)
                                           tCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATCAAATCACGTGCTTCCCGCACCGGAAGATGAGTCGGTTGTACAGGGCGCTCTCCAccag                                                             <  2:2463830/97‑1 (MQ=255)
                                                gCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGc                                                     >  2:3256056/1‑100 (MQ=255)
                                                gCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGc                                                     >  2:553762/1‑100 (MQ=255)
                                                gCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGc                                                     >  2:2429013/1‑100 (MQ=255)
                                                    gATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGc                                                 >  1:3445090/1‑100 (MQ=255)
                                                       tACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGtt                                              <  1:1065852/100‑1 (MQ=255)
                                                         cTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTc                                            >  1:3255610/1‑100 (MQ=255)
                                                         cTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTc                                            >  2:3257590/1‑100 (MQ=255)
                                                           cATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAg                                          <  2:2599424/100‑1 (MQ=255)
                                                           cATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAg                                          >  2:2109349/1‑100 (MQ=255)
                                                           cATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAg                                          <  1:3577731/100‑1 (MQ=255)
                                                              gggTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGtt                                        <  1:3832502/99‑1 (MQ=255)
                                                              gggTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTg                                       <  1:922914/100‑1 (MQ=255)
                                                              gggTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTg                                       <  1:946258/100‑1 (MQ=255)
                                                               ggTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGc                                      >  1:1510904/1‑100 (MQ=255)
                                                               ggTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGc                                      <  2:2321517/100‑1 (MQ=255)
                                                                gTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGcc                                     <  2:2123725/100‑1 (MQ=255)
                                                                gTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGcc                                     >  1:809244/1‑100 (MQ=255)
                                                                   ggTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGa                                  >  2:3035181/1‑100 (MQ=255)
                                                                   ggTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGa                                  >  2:1842033/1‑100 (MQ=255)
                                                                   ggTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGa                                  >  2:1573354/1‑100 (MQ=255)
                                                                   ggTTTCTCGTTAATAAAATCCAGGGCGTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCACCGTCTCAGTTGCCAGa                                  >  2:1917426/1‑100 (MQ=255)
                                                                    gTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGAc                                 <  2:347949/100‑1 (MQ=255)
                                                                       tctcGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCa                              >  2:3370126/1‑100 (MQ=255)
                                                                        ctcGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCa                              >  2:861967/1‑99 (MQ=255)
                                                                        ctcGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAg                             >  1:2855165/1‑100 (MQ=255)
                                                                         tcGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAgg                            >  1:2637241/1‑100 (MQ=255)
                                                                          cGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGc                           >  2:2276973/1‑100 (MQ=255)
                                                                            ttAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGcc                          <  2:2809268/99‑1 (MQ=255)
                                                                            ttAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCa                         <  1:2706704/100‑1 (MQ=255)
                                                                            ttAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCa                         >  1:2665016/1‑100 (MQ=255)
                                                                             taataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCan                        <  1:2988497/100‑2 (MQ=255)
                                                                             taataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAg                        >  1:4016334/1‑100 (MQ=255)
                                                                              aataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGc                       >  2:3847572/1‑100 (MQ=255)
                                                                               ataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                      >  1:2609074/1‑100 (MQ=255)
                                                                               ataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                      >  1:1790121/1‑100 (MQ=255)
                                                                               ataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                      >  1:362176/1‑100 (MQ=255)
                                                                                   agtCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCACCACtac                  >  2:1068338/3‑98 (MQ=255)
                                                                                     tactcatcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCg                >  1:521950/8‑100 (MQ=255)
                                                                                     tCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCg                <  1:472235/100‑1 (MQ=255)
                                                                                      actcatcTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGc               >  2:4030897/7‑100 (MQ=255)
                                                                                       cAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGcc              >  1:1903294/1‑100 (MQ=255)
                                                                                       cAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGcc              >  1:959952/1‑100 (MQ=255)
                                                                                        tcatcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCa             >  2:2081633/5‑100 (MQ=255)
                                                                                        tcatcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCa             >  2:81556/5‑100 (MQ=255)
                                                                                        aGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGACCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCa             >  1:822523/1‑100 (MQ=255)
                                                                                         gggCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAc            >  1:1234546/1‑100 (MQ=255)
                                                                                         gggCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAc            <  1:152024/100‑1 (MQ=255)
                                                                                         gggCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAc            >  1:3639950/1‑100 (MQ=255)
                                                                                         catcTTCCGGTGCCGGAAGATGCGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAc            >  2:3805878/4‑100 (MQ=255)
                                                                                         catcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGTCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAc            >  2:2084008/4‑100 (MQ=255)
                                                                                         catcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAc            >  1:1205798/4‑100 (MQ=255)
                                                                                         catcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAc            >  1:1743477/4‑100 (MQ=255)
                                                                                          atcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAcc           >  1:3698842/3‑100 (MQ=255)
                                                                                           gCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACcg          >  2:1277102/1‑100 (MQ=255)
                                                                                              tCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCgcgg       >  1:3582876/1‑100 (MQ=255)
                                                                                                 ggCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGt    >  1:3021581/1‑100 (MQ=255)
                                                                                                 ggCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGt    >  2:243464/1‑100 (MQ=255)
                                                                                                   cACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTAc  <  1:519779/100‑1 (MQ=255)
                                                                                                   cACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTAc  <  2:1234538/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
ATCTGCCATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTAC  >  NC_000913/1964989‑1965187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: