Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,971,761 T→C 23.3% T311A (ACC→GCC)  tar ← methyl‑accepting chemotaxis protein II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,971,7610TC23.3% 221.5 / 71.8 133T311A (ACC→GCC) tarmethyl‑accepting chemotaxis protein II
Reads supporting (aligned to +/- strand):  ref base T (60/41);  new base C (14/17);  total (74/59)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.01e-01

Only 100 of 135 total aligned reads displayed.

TCCACCACTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACC  >  NC_000913/1971664‑1971860
                                                                                                 |                                                                                                   
  caccacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAgc                                                                                                 >  1:131967/1‑100 (MQ=255)
  caccacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAgc                                                                                                 <  1:98275/100‑1 (MQ=255)
   accacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAgct                                                                                                <  2:4091661/100‑1 (MQ=255)
   accacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCACGGtatttg                                                                                                <  1:482369/100‑6 (MQ=255)
       cTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTc                                                                                            <  2:2097549/100‑1 (MQ=255)
       cTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTc                                                                                            <  1:192737/100‑1 (MQ=255)
        tttGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTcc                                                                                           <  2:404517/100‑1 (MQ=255)
          tGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCAt                                                                                         >  1:3894108/1‑100 (MQ=255)
           gccgccGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGGGAGCTGCTCCAtn                                                                                        <  2:2559715/100‑2 (MQ=255)
            ccgccgTGCTGGGCGGTGTCGGGGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGc                                                                                       >  2:1705414/1‑100 (MQ=255)
            ccgccgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGc                                                                                       <  2:1118842/100‑1 (MQ=255)
                cgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTggc                                                                                   >  1:2448417/1‑100 (MQ=255)
                 gTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTggcg                                                                                  >  1:2556372/1‑100 (MQ=255)
                  tcttGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTggcgg                                                                                 <  1:3209108/97‑1 (MQ=255)
                  tGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTggcg                                                                                  <  2:3252760/99‑1 (MQ=255)
                   gCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTggcggc                                                                                <  2:492863/100‑1 (MQ=255)
                   gCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTggcggc                                                                                <  1:1779890/100‑1 (MQ=255)
                    cTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCa                                                                               <  1:1009197/100‑1 (MQ=255)
                     tGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTggcggaga                                                                              >  2:3209088/1‑97 (MQ=255)
                       ggCGGTGTCGGAGGCGCTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGt                                                                             <  2:261976/99‑1 (MQ=255)
                       ggCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGtt                                                                            <  1:3537133/100‑1 (MQ=255)
                        gCGGTGTCGGAGGCGCTTGGCGCCAGTTGGGAGGCCTGGCGGGCGTTATCGGCGTGTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGt                                                                             <  2:2005070/98‑1 (MQ=38)
                        gCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGtt                                                                            >  2:2838429/1‑99 (MQ=255)
                        gCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGtt                                                                            <  2:4088850/99‑1 (MQ=255)
                          ggTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTttct                                                                         >  1:3751364/1‑100 (MQ=255)
                          ggTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTttct                                                                         <  1:2872769/100‑1 (MQ=255)
                          ggTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTttct                                                                         >  2:1631398/1‑100 (MQ=255)
                            tgtCGGGGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTttcttc                                                                       <  2:3696941/100‑1 (MQ=255)
                            tgtCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTttcttc                                                                       <  1:3400290/100‑1 (MQ=255)
                             gtCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTcc                                                                      >  1:55075/1‑100 (MQ=255)
                             gtCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTGTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTttgttc                                                                       >  2:3703971/1‑99 (MQ=255)
                               cGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCa                                                                     >  2:3037644/1‑99 (MQ=255)
                                ggAGGCACTTTGTGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAgc                                                                   >  1:686799/1‑100 (MQ=255)
                                ggAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAgc                                                                   >  1:2908935/1‑100 (MQ=255)
                                ggAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAgc                                                                   >  1:3812255/1‑100 (MQ=255)
                                ggAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTATTCCAg                                                                    >  2:2428266/1‑99 (MQ=255)
                                 gAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAgcg                                                                  >  2:2965868/1‑100 (MQ=255)
                                 gAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAgcg                                                                  >  2:2286469/1‑100 (MQ=255)
                                   ggCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGcgcg                                                                <  1:440344/100‑1 (MQ=255)
                                   ggCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGcgcg                                                                >  1:3618699/1‑100 (MQ=255)
                                    gCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCgg                                                               <  2:3048313/100‑1 (MQ=255)
                                     cACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGcgcgaa                                                              >  2:197332/1‑98 (MQ=255)
                                       cTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATg                                                            <  2:3424671/100‑1 (MQ=255)
                                          tGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCct                                                         >  2:915793/1‑100 (MQ=255)
                                           gcgcCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctg                                                        >  1:1564107/1‑100 (MQ=255)
                                            cgcCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgc                                                       >  1:2135425/1‑100 (MQ=255)
                                             gcCAGGTGCGAGGCCTGGCGGGCGTTATCGGCGGTTTGCTTCACTGGCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgc                                                       <  2:1641846/99‑1 (MQ=255)
                                              ccAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgctg                                                     <  1:365874/100‑1 (MQ=255)
                                               cAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTTTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgct                                                      <  2:3719773/98‑1 (MQ=255)
                                               cAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGt                                                    <  1:117255/100‑1 (MQ=255)
                                               cAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGt                                                    <  1:1631410/100‑1 (MQ=255)
                                                aGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGtt                                                   <  2:1434928/100‑1 (MQ=255)
                                                aGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCATCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGtt                                                   >  1:2912914/1‑100 (MQ=255)
                                                  ttGCGTGTCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGCCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTc                                                  <  2:3470962/99‑1 (MQ=38)
                                                  ttGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCa                                                 <  2:2132184/100‑1 (MQ=255)
                                                    gCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGt                                               >  1:2871831/1‑100 (MQ=255)
                                                      gAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGt                                               >  2:933499/1‑98 (MQ=255)
                                                      gAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTAc                                             >  1:3047554/1‑100 (MQ=255)
                                                         gCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACggg                                          <  1:190524/100‑1 (MQ=255)
                                                           cTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTTCTCCATGCTGGCGGCAGTTTCATACAGCGCGGACGCATGCCGTTCCGTACGgga                                         >  2:1561690/1‑99 (MQ=25)
                                                           cTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGggag                                        >  2:2656338/1‑100 (MQ=255)
                                                           cTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGggag                                        <  1:3346255/100‑1 (MQ=255)
                                                             ggcgggcgTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGggagg                                       >  2:3115856/1‑99 (MQ=255)
                                                              gcgggcgTTATGGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTc                                                  <  2:3592775/87‑1 (MQ=255)
                                                                 ggcgTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCCGCGCGGATGCCTGCTGTTCCGTACGGGAGGAAAGa                                  >  2:2185628/1‑100 (MQ=255)
                                                                 ggcgTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGa                                  <  1:3881413/100‑1 (MQ=255)
                                                                   cgTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATc                                <  1:2345968/100‑1 (MQ=255)
                                                                   cgTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATc                                >  1:1924249/1‑100 (MQ=255)
                                                                     ttATCGGCGTTTTGCTTCACTGTCTCGGCGAGCTGCTCCATGCTGGCGGCAGTTTATGCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCgt                              <  2:3951676/100‑2 (MQ=38)
                                                                     ttATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCgg                              <  1:1019526/100‑1 (MQ=255)
                                                                      tATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCgg                              <  2:3929638/99‑1 (MQ=255)
                                                                       aTCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGgtg                            >  2:2430451/1‑100 (MQ=255)
                                                                        tCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGgtgt                           >  1:3473614/1‑100 (MQ=255)
                                                                        tCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTGTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGgtgt                           <  2:3302171/100‑1 (MQ=255)
                                                                         cGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGtt                          >  2:2843438/1‑100 (MQ=255)
                                                                         cGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGtt                          >  2:3198831/1‑100 (MQ=255)
                                                                          ggCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTg                         <  2:3174953/100‑1 (MQ=255)
                                                                          ggCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTg                         <  1:3757473/100‑1 (MQ=255)
                                                                          ggCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTg                         >  1:3969099/1‑100 (MQ=255)
                                                                            cGTTTTGCTTCACTGTCGCGTTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGc                        <  2:771283/99‑1 (MQ=255)
                                                                            cGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGcc                       >  2:1633883/1‑100 (MQ=255)
                                                                             gTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGcc                       <  2:800579/99‑1 (MQ=255)
                                                                              ttttGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAGGATCGGTGTTGCccg                     <  2:3845495/100‑1 (MQ=255)
                                                                              ttttGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCccg                     <  1:70747/100‑1 (MQ=255)
                                                                              ttttGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCccg                     <  2:587610/100‑1 (MQ=255)
                                                                               tttGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCccgc                    >  2:1771540/1‑100 (MQ=255)
                                                                                 tGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCccgccg                  <  1:2313901/100‑1 (MQ=255)
                                                                                  gCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCcgccgc                 <  2:1460244/100‑1 (MQ=255)
                                                                                  gCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCcgccgc                 >  1:310878/1‑100 (MQ=255)
                                                                                   cTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCa                >  1:708310/1‑100 (MQ=255)
                                                                                    ttCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGCGTTGCCCGCCGCaa               >  1:3949709/1‑100 (MQ=255)
                                                                                     tCACTGTCGCGGTGAGCTGCTCCCTGCTGGCGGCAGCTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGGGGCATGATCGGTGGTGCCCGCCGCAAt              >  2:1780196/1‑100 (MQ=255)
                                                                                     tCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAAt              <  1:915799/100‑1 (MQ=255)
                                                                                     tCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAAt              >  1:1570068/1‑100 (MQ=255)
                                                                                       aCTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAAttt            >  1:4040767/1‑100 (MQ=255)
                                                                                        cTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGCGGAAACATCGGTGTTGCCCGCCGCAAttt            >  2:748213/1‑99 (MQ=255)
                                                                                         tGTCGCGGTGAGCTGCGCCATGCTGGCGGCAGTTTCTTCCATCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCa          <  1:1375551/100‑1 (MQ=255)
                                                                                             gcgGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACggg      >  1:2586689/1‑100 (MQ=255)
                                                                                                gTGAGCTGCTCCATGCTGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCACTTTCACGGGTAcc  >  2:675480/1‑100 (MQ=255)
                                                                                                 tGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTAcc  <  1:3094420/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
TCCACCACTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGGTACC  >  NC_000913/1971664‑1971860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: