Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,988,339 C→T 56.9% Q40* (CAG→TAG)  yecR → lipoprotein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,988,3390CT56.9% 42.5 / 130.0 109Q40* (CAG→TAG) yecRlipoprotein, function unknown
Reads supporting (aligned to +/- strand):  ref base C (23/24);  new base T (35/27);  total (58/51)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

Only 100 of 111 total aligned reads displayed.

TCTTTCATTAATAACGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCTATACCCACGCGGTCCCCTTTG  >  NC_000913/1988242‑1988438
                                                                                                 |                                                                                                   
  tttCATTAATAACGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGCGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGc                                                                                                 >  2:4022490/1‑100 (MQ=255)
  tttCATTAATAACGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGc                                                                                                 >  1:1115076/1‑100 (MQ=255)
   ttCGTTAATAGCGCTGGGGGGGTGTACGGTTACTCGCCAAGCACGTGTGAGCGAAGTTGACGCGGCAACAGGTATTGGACGGTTAGTTTATGACCAGGc                                                                                                 <  2:3537562/99‑1 (MQ=255)
   ttCATTAATAACGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCt                                                                                                >  1:407021/1‑100 (MQ=255)
   ttCATTAATAACGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCt                                                                                                >  1:16532/1‑100 (MQ=255)
   ttCATTAATAACGCTGGCGGGGTGTACGGTTACGCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGc                                                                                                 <  2:1327347/99‑1 (MQ=255)
    tCATTAATAACGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCtt                                                                                               >  1:96964/1‑100 (MQ=255)
     cATTAATAACGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCttt                                                                                              >  2:1415945/1‑100 (MQ=255)
      aTTAATAACGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCtttt                                                                                             >  1:1268675/1‑100 (MQ=255)
       ttAATAACGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCttttt                                                                                            <  1:1541166/100‑1 (MQ=255)
        taataaCGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCtttttt                                                                                           <  2:3064212/100‑1 (MQ=255)
          ataaCGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTgc                                                                                         >  2:3153511/1‑100 (MQ=255)
          ataaCGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTgc                                                                                         <  2:2834808/100‑1 (MQ=255)
          ataaCGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTgc                                                                                         >  2:1338885/1‑100 (MQ=255)
          ataaCGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTgc                                                                                         >  1:2896742/1‑100 (MQ=255)
            accGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTg                                                                                          <  2:2462446/95‑1 (MQ=255)
            aaCGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTgcag                                                                                       >  1:2136551/1‑100 (MQ=255)
                cTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATg                                                                                   >  1:1259436/1‑100 (MQ=255)
                  ggCGGAGTGTACGGTTACTCTCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGcc                                                                                 <  1:2868933/100‑1 (MQ=255)
                     ggggTGTACGGTTACTCGCCAAGCCCATGTCAGCGAAGTTGACGTGGCAACATGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCa                                                                               <  2:468933/99‑1 (MQ=255)
                       ggTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATAc                                                                            <  2:2134645/100‑1 (MQ=255)
                        gtgtACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATAcc                                                                           <  2:407018/100‑1 (MQ=255)
                        gtgtACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAACATGCCCATAcc                                                                           <  2:2364945/100‑1 (MQ=255)
                        gtgtACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATAcc                                                                           <  1:753268/100‑1 (MQ=255)
                         tgtACGTCTACTCGCCAAGTCAATGTGAGCGAAGTTAACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTATTGCACCATGCCCATAc                                                                            <  2:2122198/98‑1 (MQ=255)
                           tACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGAt                                                                        >  2:1497119/1‑100 (MQ=255)
                           tACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGAt                                                                        <  1:1023858/100‑1 (MQ=255)
                           tACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGAt                                                                        >  2:444090/1‑100 (MQ=255)
                            aCGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATc                                                                       >  2:602205/1‑100 (MQ=255)
                            aCGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATc                                                                       <  1:2708140/100‑1 (MQ=255)
                            aCGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATc                                                                       >  2:4065205/1‑100 (MQ=255)
                            aCGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATc                                                                       >  1:4133771/1‑100 (MQ=255)
                             cGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCg                                                                      <  1:3660857/100‑1 (MQ=255)
                             cGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCg                                                                      >  1:110182/1‑100 (MQ=255)
                               gTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGtt                                                                    <  2:816356/100‑1 (MQ=255)
                                ttACTCGCCAAGCCCATGTGAGCGAAGTTGACGATGCAATAGGTATTGTACGGTTAGTTTATGACTAGGTTTTTTTGCAGCATGCCCATACCGATCGTTa                                                                   <  2:1914524/100‑1 (MQ=255)
                                  aCTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATg                                                                 <  2:516152/100‑1 (MQ=255)
                                   cTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCTTACCTATACCGATCGTTAttt                                                                >  2:1801585/1‑98 (MQ=255)
                                   cTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGt                                                                >  1:3090407/1‑100 (MQ=255)
                                   cTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATACCCATACCGATCGTTATGt                                                                >  2:3180860/1‑100 (MQ=255)
                                   cTCGCCAAGCCCATGTGAGCGAAGTTCACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGt                                                                >  1:2516276/1‑100 (MQ=255)
                                    tCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTc                                                               <  1:4065232/100‑1 (MQ=255)
                                     cGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCa                                                              >  2:3016732/1‑100 (MQ=255)
                                      gCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAg                                                             >  1:3886786/1‑100 (MQ=255)
                                           gCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTcgcg                                                        >  2:1463475/1‑100 (MQ=255)
                                            cccATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCgg                                                       <  1:2160963/100‑1 (MQ=255)
                                             ccATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCggg                                                      >  1:968263/1‑100 (MQ=255)
                                             ccATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCggg                                                      <  2:244160/100‑1 (MQ=255)
                                             ccATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCggg                                                      >  2:2466886/1‑100 (MQ=255)
                                             ccATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCggg                                                      >  2:412452/1‑100 (MQ=255)
                                              cATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCgggn                                                     <  2:3220372/100‑2 (MQ=255)
                                              cATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGa                                                     <  2:3220436/100‑1 (MQ=255)
                                               aTGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGAt                                                    >  1:2205616/1‑100 (MQ=255)
                                               aTGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGAt                                                    >  1:2977806/1‑100 (MQ=255)
                                                   gAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGcc                                                <  1:340331/100‑1 (MQ=255)
                                                   gAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGcc                                                <  1:2363159/100‑1 (MQ=255)
                                                   gAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTCGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGAAATCGcc                                                <  2:1856498/100‑1 (MQ=255)
                                                    aGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCg                                               <  1:2675562/100‑1 (MQ=255)
                                                     gCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTAGGATCCGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGa                                              <  2:2641208/100‑1 (MQ=255)
                                                       gAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATc                                            <  1:3733657/100‑1 (MQ=255)
                                                        aaGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATCCCGATCGTTATGTCAGTCGCGGGATCGCCGATcg                                           <  1:2155066/100‑1 (MQ=255)
                                                          gTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATcgcg                                         >  1:485298/1‑100 (MQ=255)
                                                          gTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATcgcg                                         <  1:3702981/100‑1 (MQ=255)
                                                          gTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATcgcg                                         >  1:3660255/1‑100 (MQ=255)
                                                           tttaCGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCgcgc                                        <  2:2459598/97‑1 (MQ=255)
                                                            tGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCa                                       >  1:1967322/1‑100 (MQ=255)
                                                             gACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCAt                                      >  2:2352895/1‑100 (MQ=255)
                                                             gACGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGCGATCGGCGATCccgcg                                       <  2:3510401/99‑2 (MQ=255)
                                                              aCGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATg                                     <  1:3304447/100‑1 (MQ=255)
                                                              aCGCGGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGCGATCGGCGATCccgcgac                                     <  2:636454/100‑4 (MQ=255)
                                                              aCGCGGCAACAGGTAGTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCAt                                      <  2:2353830/99‑1 (MQ=255)
                                                               cgcgGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGc                                    >  2:1728080/1‑100 (MQ=255)
                                                               cgcgGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGc                                    >  1:47204/1‑100 (MQ=255)
                                                               cgcgGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGCGATCGGCGATCccgcgac                                     <  2:2965079/99‑4 (MQ=255)
                                                               cgcgGCAACAGGTAGTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGCGATCGGCGATCccgcgact                                    <  2:4133935/100‑5 (MQ=255)
                                                                 cgGCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCca                                  >  1:2142658/1‑100 (MQ=255)
                                                                  ggCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCcag                                 <  1:1552403/100‑1 (MQ=255)
                                                                  ggCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCcag                                 <  1:3205805/100‑1 (MQ=255)
                                                                   gCAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCcagc                                >  1:3478351/1‑100 (MQ=255)
                                                                    cAACAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCcagca                               >  1:1987153/1‑100 (MQ=255)
                                                                    cAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGAATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCTCATGCcagca                               >  2:1052658/1‑100 (MQ=255)
                                                                       cAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGa                            >  1:525965/1‑100 (MQ=255)
                                                                       cAGGTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGa                            >  2:719947/1‑100 (MQ=255)
                                                                         ggTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAg                          >  1:1498923/1‑100 (MQ=255)
                                                                         ggTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAg                          >  1:759668/1‑100 (MQ=255)
                                                                         ggTATTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAg                          <  2:3493601/100‑1 (MQ=255)
                                                                         ggTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAg                          >  1:1876881/1‑100 (MQ=255)
                                                                         ggTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAg                          >  2:3107197/1‑100 (MQ=255)
                                                                          gTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAgg                         <  1:3419990/100‑1 (MQ=255)
                                                                            aTTGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCt                       >  1:2127003/1‑100 (MQ=255)
                                                                            aTTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCATCAGGAAgg                         >  2:2605684/1‑98 (MQ=255)
                                                                              tGTACGGTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCtat                     >  2:1303298/1‑100 (MQ=255)
                                                                                 aCGGTTAGTTTATGACTAGGCTTTTTTTTTGCTTTTTTATACCGATCGTTATGTCCGTCGTCTGATCTCCGATCGCGCATGCCAGCAGGAAGGCTacac                   >  2:1882665/1‑99 (MQ=255)
                                                                                    gTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCTATACCCAc               >  2:1305730/1‑100 (MQ=255)
                                                                                    gTTAGTTTATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCTATACCCAc               >  1:964282/1‑100 (MQ=255)
                                                                                           tATGACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCTATACCCACGCGGTcc        <  1:1704541/100‑1 (MQ=255)
                                                                                              gACTAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCTATACCCACGCGGTccc       >  2:4042801/1‑98 (MQ=255)
                                                                                              gACCAGGCTTTTTAGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCTATACCCACGCGGTCCCCt     >  1:2798291/1‑100 (MQ=255)
                                                                                                 cAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCTATACCCACGCGGTCCCCTTTg  >  2:352695/1‑100 (MQ=255)
                                                                                                 cAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCTATACCCACGCGGTCCCCTTTg  <  2:4022601/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
TCTTTCATTAATAACGCTGGCGGGGTGTACGGTTACTCGCCAAGCCCATGTGAGCGAAGTTGACGCGGCAACAGGTATTGTACGGTTAGTTTATGACCAGGCTTTTTTGCAGCATGCCCATACCGATCGTTATGTCAGTCGCGGGATCGCCGATCGCGCATGCCAGCAGGAAGGCTATACCCACGCGGTCCCCTTTG  >  NC_000913/1988242‑1988438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: