Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,044,638 C→A 64.3% intergenic (+14/‑300) asnT → / → yeeJ tRNA‑Asn/putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,044,6380CA64.3% 97.0 / 121.3 109intergenic (+14/‑300)asnT/yeeJtRNA‑Asn/putative adhesin
Reads supporting (aligned to +/- strand):  ref base C (24/15);  new base A (30/40);  total (54/55)
Fisher's exact test for biased strand distribution p-value = 7.38e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

Only 100 of 111 total aligned reads displayed.

ACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGG  >  NC_000913/2044542‑2044737
                                                                                                |                                                                                                   
acacGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCt                                                                                                  >  2:2046288/1‑100 (MQ=40)
 cacGATTCCTCTGTAGTTCAGTCAGTAGAATGGCGGACTGTTAATCCGTATGTCACTGGTTCGCGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCt                                                                                                  >  2:3672553/1‑99 (MQ=11)
  acGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCttt                                                                                                >  2:316845/1‑100 (MQ=255)
   cGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCtttt                                                                                               <  1:3195487/100‑1 (MQ=255)
    gATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCtttt                                                                                               <  2:3849406/99‑1 (MQ=255)
     aTTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCtttttt                                                                                             <  1:161840/100‑1 (MQ=255)
        ccTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAgc                                                                                          <  2:464292/100‑1 (MQ=255)
         ctctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAgcg                                                                                         <  2:2082673/100‑1 (MQ=255)
         ctctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAgcg                                                                                         >  1:850975/1‑100 (MQ=255)
         ctctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAgcg                                                                                         <  2:3006244/100‑1 (MQ=255)
          tctGAAGTTCAGTCGGTAGAACGGCGTACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTTAGAGGAGCCAAAATCTAAAAATTCGCTTTTTTAgcg                                                                                         <  2:1712151/99‑1 (MQ=17)
           ctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCa                                                                                       >  1:1115503/1‑100 (MQ=255)
             gTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAAt                                                                                     >  1:3902237/1‑100 (MQ=255)
             gTAGTTCAGTAGGTAGAACGGCGGAGTGTTAATCCGTATGTTACTGGTTTGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAAt                                                                                     <  2:4079225/100‑1 (MQ=17)
               aGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTAAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGt                                                                                   >  2:2893627/1‑100 (MQ=255)
                gctCAGTCGGTAGAACGGCTGACTGTTAATCCGTGTGTCACTGTTTTGAGTGCAGTCCGAGGAGCCAAATTCTACAAATTAGCTTTTGTAGCGCTATGTc                                                                                  >  2:2466005/3‑100 (MQ=17)
                gTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTc                                                                                  <  1:2159634/100‑1 (MQ=255)
                   cAGACGCTAGAACGGCGAATTATGAATCCGTATGACTCTGGTCCGTGTCTTGTAAGAGGAGCCAAATTCGAAAAATTCGCTTTCTTTGCTCAATGTCACt                                                                               >  2:1213678/1‑100 (MQ=12)
                    aGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTg                                                                              <  2:923731/100‑1 (MQ=255)
                     gTCGGTAGAACGGCGGACTGTTAATCCGTCTGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCGTTTTTAGCGCAATGTCACTg                                                                              >  2:3524354/1‑99 (MQ=255)
                      tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGAc                                                                            <  1:1020289/100‑1 (MQ=255)
                         gTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCtt                                                                         <  1:3715222/100‑1 (MQ=255)
                         gTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCtt                                                                         >  1:3044856/1‑100 (MQ=255)
                         gTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCtt                                                                         <  1:3252825/100‑1 (MQ=255)
                           aGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAg                                                                       <  2:963413/100‑1 (MQ=255)
                            gAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGt                                                                      >  1:3636124/1‑100 (MQ=255)
                             aaCGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGtt                                                                     >  1:68311/1‑100 (MQ=255)
                             aaCGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGtt                                                                     <  1:3573642/100‑1 (MQ=255)
                             aaCGGCCGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGtt                                                                     <  2:2569291/100‑1 (MQ=255)
                                ggcggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGaa                                                                  <  2:19080/100‑1 (MQ=255)
                                 gcggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAAc                                                                 >  1:3791167/1‑100 (MQ=255)
                                 gcggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAAc                                                                 >  1:241695/1‑100 (MQ=255)
                                   ggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACAt                                                               >  2:3923618/1‑100 (MQ=255)
                                     aCTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTg                                                             >  1:2695732/1‑100 (MQ=255)
                                      cTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGt                                                            >  2:291860/1‑100 (MQ=255)
                                       tGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGtt                                                           >  1:3478155/1‑100 (MQ=255)
                                        gTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGttt                                                          >  1:2291987/1‑100 (MQ=255)
                                         ttAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGtttt                                                         <  1:24584/100‑1 (MQ=255)
                                          tAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGttttt                                                        <  2:3392559/100‑1 (MQ=255)
                                           aaTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGtttttt                                                       <  1:1548194/100‑1 (MQ=255)
                                             tCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTaa                                                     <  1:1000363/100‑1 (MQ=255)
                                              ccGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAAc                                                    <  2:1689359/100‑1 (MQ=255)
                                               cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACg                                                   <  2:1789758/100‑1 (MQ=255)
                                                  atatcatttgTTTGAGTCTTGTCAGAGGAGAAAAAAAAAAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGa                                                 <  2:3806544/90‑1 (MQ=255)
                                                  aTGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGAt                                                <  1:2307367/100‑1 (MQ=255)
                                                    gTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAg                                              >  1:1132033/1‑100 (MQ=255)
                                                    gTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAg                                              >  2:1468386/1‑100 (MQ=255)
                                                    gTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAg                                              >  1:899529/1‑100 (MQ=255)
                                                     tCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGc                                             <  2:3770379/100‑1 (MQ=255)
                                                      cACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCg                                            >  2:1006966/1‑100 (MQ=255)
                                                      cACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCg                                            >  2:1301910/1‑100 (MQ=255)
                                                      cACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCg                                            >  2:3222402/1‑100 (MQ=255)
                                                      cACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCg                                            <  2:3143449/100‑1 (MQ=255)
                                                          ggTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGtt                                        >  2:2673600/1‑100 (MQ=255)
                                                          ggTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGtt                                        >  1:2863170/1‑100 (MQ=255)
                                                          ggTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGtt                                        >  2:2735345/1‑100 (MQ=255)
                                                          ggTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGtt                                        >  2:3017978/1‑100 (MQ=255)
                                                          ggTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGtt                                        >  1:4026363/1‑100 (MQ=255)
                                                           gTTCGAGTTCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGttt                                       <  2:3588405/100‑1 (MQ=255)
                                                           gTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGttt                                       >  1:1718315/1‑100 (MQ=255)
                                                            ttCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGttt                                       >  2:1547776/1‑99 (MQ=255)
                                                            ttCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGCTGAACATTGTTTTTTAACGGATAGCGGGtttt                                      <  1:3926184/100‑1 (MQ=255)
                                                            ttCGAGTCCAATCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGtttt                                      <  1:1648371/100‑1 (MQ=255)
                                                              cGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTa                                    <  1:2146228/100‑1 (MQ=255)
                                                              cGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTa                                    >  1:3400230/1‑100 (MQ=255)
                                                              cGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTa                                    >  2:3386673/1‑100 (MQ=255)
                                                              cGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTa                                    <  1:2442021/100‑1 (MQ=255)
                                                               gAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGAGGGttttt                                     >  2:2608242/1‑98 (MQ=255)
                                                                aGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGATCATTGTTTTTTAACGGATAGCGGGTTTTTAAc                                  <  1:3925903/100‑1 (MQ=255)
                                                                aGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAAc                                  <  2:1111887/100‑1 (MQ=255)
                                                                aGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAAc                                  >  1:730598/1‑100 (MQ=255)
                                                                aGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAAc                                  >  1:301407/1‑100 (MQ=255)
                                                                aGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAAc                                  <  1:411017/100‑1 (MQ=255)
                                                                aGTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAAc                                  <  2:315389/100‑1 (MQ=255)
                                                                 gTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACa                                 >  2:1405037/1‑100 (MQ=255)
                                                                 gTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACa                                 >  2:1213179/1‑100 (MQ=255)
                                                                 gTCCAGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTGTTTAAc                                  >  1:1161554/1‑99 (MQ=255)
                                                                  tCCTGTTAGAGCAGCCAAATTCTAAATATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAAc                                  <  2:3497463/98‑1 (MQ=255)
                                                                     aGTCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCtt                             >  1:724868/1‑100 (MQ=255)
                                                                       tCAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTaa                           <  1:670400/100‑1 (MQ=255)
                                                                        cAGAGGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAg                          <  2:3035006/100‑1 (MQ=255)
                                                                         agagGAGCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAgc                         <  1:2160265/100‑1 (MQ=255)
                                                                          gaggagCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAgcg                        <  1:2893643/100‑1 (MQ=255)
                                                                          gaggagCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAgcg                        <  1:455764/100‑1 (MQ=255)
                                                                             gagCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGccn                     <  2:2599210/100‑2 (MQ=255)
                                                                              agCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCt                    >  1:416210/1‑100 (MQ=255)
                                                                              agCCAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACAGCTTaatttccc                     >  2:3822696/1‑93 (MQ=255)
                                                                               gCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTc                   >  1:2768243/1‑100 (MQ=255)
                                                                                ccAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCg                  >  2:1179344/1‑100 (MQ=255)
                                                                                ccAAATTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCg                  <  1:1149165/100‑1 (MQ=255)
                                                                                   aaTTCTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGAcc               <  1:2347438/100‑1 (MQ=255)
                                                                                    attagaaagaTTAGCTTGTTGAGCGCAGTGGCACTGGCCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTGAACATCTTAAGCGCCCTCGAcc               <  2:3313442/94‑1 (MQ=255)
                                                                                       cTAAAAATTAGCTTTTTTAGCGCAATGTCACTGACATTAGTTGAAAATTGTTTTATAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTa           >  1:957929/1‑100 (MQ=255)
                                                                                        tAAAAATTAGCTTTTTTAGCGCAATGTCACTGGCCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTAt          <  2:1100408/100‑1 (MQ=255)
                                                                                        tAAAAATTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTAt          >  1:775406/1‑100 (MQ=255)
                                                                                          aaaaTTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATgg        <  1:2490203/100‑1 (MQ=255)
                                                                                            aaTTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGtt      <  2:691354/100‑1 (MQ=255)
                                                                                            aaTTAGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGAGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGt       <  2:821542/99‑1 (MQ=255)
                                                                                                cGCTTTTTTAGCGCAATGTCACTGACCTTGGTGGACCATTGCTTTGTAACGGATAACGGGTTTTTCAACTCTTAGACGCCCTCGACCTTTATGGTTGAgg  >  2:2766255/1‑100 (MQ=255)
                                                                                                cGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAgg  >  2:1820937/1‑100 (MQ=255)
                                                                                                |                                                                                                   
ACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGG  >  NC_000913/2044542‑2044737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: