Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,071,321 A→G 100% intergenic (+110/‑218) yeeP → / → flu pseudogene, CP4‑44 prophage; 50S ribosome‑binding GTPase family;Phage or Prophage Related; putative histone/CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,071,3210AG96.9% 412.9 / 1.3 127intergenic (+110/‑218)yeeP/flupseudogene, CP4‑44 prophage; 50S ribosome‑binding GTPase family;Phage or Prophage Related; putative histone/CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base G (64/59);  total (66/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

Only 100 of 128 total aligned reads displayed.

GATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAAC  >  NC_000913/2071225‑2071417
                                                                                                |                                                                                                
gATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGata                                                                                               >  1:103622/1‑100 (MQ=255)
gATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGata                                                                                               <  2:4034713/100‑1 (MQ=255)
gATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGata                                                                                               <  1:3176563/100‑1 (MQ=255)
gATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGata                                                                                               <  1:2188625/100‑1 (MQ=255)
  tGCCCTCACTGACTCTGAGTCTGCTCACAAAAGCACGGTTTTCGTTACTGTCTCTCTTGTCCCTGCAATAGCTCAATAATAGAATAAAACGATCGATATc                                                                                             <  2:2213876/100‑1 (MQ=255)
   gCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCt                                                                                            >  1:2845593/1‑100 (MQ=255)
    ccctccctGACTCTGAGTCTGCTCACCAAATCACGGTTTTCGGTACCGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTa                                                                                           <  1:2605548/100‑1 (MQ=255)
    ccctccctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTa                                                                                           >  1:863517/1‑100 (MQ=255)
      ctccctGACTCTGAGTCTGCTCACCAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGACTAAAACGATCGATATCTAtt                                                                                         >  2:3270402/1‑100 (MQ=255)
      ctccctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTAtt                                                                                         >  2:461144/1‑100 (MQ=255)
        ccctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTAtttt                                                                                       <  1:4090974/100‑1 (MQ=255)
        ccctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCAATATCTAtttt                                                                                       >  1:3770803/1‑100 (MQ=255)
         cctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTAtttt                                                                                       <  1:2613147/99‑1 (MQ=255)
         cctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTa                                                                                      >  2:2807528/1‑100 (MQ=255)
          ctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTat                                                                                     >  2:688065/1‑100 (MQ=255)
           tGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTatc                                                                                    <  2:2129327/100‑1 (MQ=255)
            gACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTatcg                                                                                   <  1:2314226/100‑1 (MQ=255)
              ctctGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTatcgat                                                                                 >  1:3368037/1‑100 (MQ=255)
              ctctGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTatcgat                                                                                 >  2:3762650/1‑100 (MQ=255)
              ctctGAGTCTGCTCACAAAAGCACTGTGTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTatcgat                                                                                 <  1:1507140/100‑1 (MQ=255)
               tctGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTatcgatc                                                                                >  2:1642750/1‑100 (MQ=255)
                ctGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCACGCAATAGCTCAATAATAGAATAAAACGCACGATATATATTTTatccat                                                                                 >  2:2478567/1‑98 (MQ=255)
                 tGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGTTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGt                                                                              <  2:4057743/100‑1 (MQ=255)
                 tGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGt                                                                              <  2:3019502/100‑1 (MQ=255)
                  gAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCAATATCTATTTTATCGATCGtt                                                                             <  1:1293922/100‑1 (MQ=255)
                   aGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGttt                                                                            >  1:1025818/1‑100 (MQ=255)
                    gTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTta                                                                           <  1:232679/100‑1 (MQ=255)
                    gTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTta                                                                           >  1:1118049/1‑100 (MQ=255)
                    ccgatcTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTta                                                                           <  2:2156905/95‑1 (MQ=255)
                       tGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTatat                                                                        <  2:290922/100‑1 (MQ=255)
                         cTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATatc                                                                       <  2:2778556/99‑1 (MQ=255)
                           cacaAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCACTAATAGAATAAAACGATCGATACCTATTTTATCCATCGTTTCTatcgat                                                                    >  2:1336085/1‑100 (MQ=255)
                           cacaAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTtaagatcgg                                                                    >  1:2156918/1‑93 (MQ=255)
                           cacaAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATatcgat                                                                    >  1:39698/1‑100 (MQ=255)
                            acaAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATatcgatc                                                                   <  1:1107797/100‑1 (MQ=255)
                             caAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATatcgatcg                                                                  <  2:1956619/100‑1 (MQ=255)
                             caAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATatcgatcg                                                                  >  1:3863577/1‑100 (MQ=255)
                               aaaGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATcgatcgat                                                                >  2:1584572/1‑100 (MQ=255)
                               aaaGCACTGTTTTCGTTACTGTCTCTCTTGCCCCTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTAGATcgatcgat                                                                >  2:2723249/1‑100 (MQ=255)
                                 aGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATaa                                                              <  2:210525/100‑1 (MQ=255)
                                  tgaCTGTTTTCGTTACTGTCTATCTGTGCCGTGCAATATCTAAATAACAGAATAAAACGATCGATATCTAGTTTAGCGATCGATATAAACGATCGATaaa                                                             <  2:657190/98‑2 (MQ=16)
                                  gCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAg                                                             >  2:3020047/1‑100 (MQ=255)
                                  gCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAg                                                             >  2:4058864/1‑100 (MQ=255)
                                   cACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGc                                                            >  1:1673859/1‑100 (MQ=255)
                                   cACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGc                                                            >  1:2555383/1‑100 (MQ=255)
                                    aCTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCt                                                           <  2:2624939/100‑1 (MQ=255)
                                     cTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGCTCGTTTATATCGATCGATAAGCta                                                          >  1:2138106/1‑100 (MQ=255)
                                      ttttttCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATTGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCta                                                          <  1:2851405/97‑1 (MQ=255)
                                      tGTTTTTGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATATAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCt                                                           <  2:3488663/98‑1 (MQ=255)
                                      tGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATCGCTCAATAATAGAATAAAACGATCGCTATCTATTTTATCAATCGTTTATATCGATCGATAATCta                                                          >  2:1202152/1‑99 (MQ=255)
                                       gTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCtaat                                                        >  2:2334616/1‑100 (MQ=255)
                                       gTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCtaat                                                        >  2:2226389/1‑100 (MQ=255)
                                       gTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATAGATCGTTTATATCGATCGATAAGCta                                                          >  2:1614783/1‑98 (MQ=255)
                                         tttCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCtaataa                                                      <  2:1109872/100‑1 (MQ=255)
                                           ncGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCtaataata                                                    >  1:1258924/2‑100 (MQ=255)
                                            cGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCtaataata                                                    <  2:1736153/99‑1 (MQ=255)
                                             gTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAAc                                                  >  2:2855697/1‑100 (MQ=255)
                                               tACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATAGATAAGCtaataataccat                                                >  1:2829502/1‑96 (MQ=255)
                                                aCTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAAcatt                                               >  1:2148778/1‑97 (MQ=255)
                                                aCTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAAcatt                                               >  1:3011569/1‑97 (MQ=255)
                                                  tGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTg                                             >  1:2034561/1‑100 (MQ=255)
                                                  tGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTg                                             <  1:1865641/100‑1 (MQ=255)
                                                    tctctcTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTc                                           <  2:3757604/100‑1 (MQ=255)
                                                       ctctTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTg                                             >  2:225164/1‑95 (MQ=255)
                                                       ctctTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGt                                        <  2:39698/100‑1 (MQ=255)
                                                       ctctTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGt                                        >  1:4039080/1‑100 (MQ=255)
                                                        tcgtGTCCGTGCAATAGCTCAATAATAGAATAAAACTATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTa                                       <  2:3141976/97‑1 (MQ=255)
                                                         ctTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTaa                                      >  1:3578652/1‑100 (MQ=255)
                                                                 tGCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGcaca                              <  2:3151066/100‑1 (MQ=255)
                                                                  gCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAg                             <  1:3659918/100‑1 (MQ=255)
                                                                  gCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAg                             >  1:1284073/1‑100 (MQ=255)
                                                                  gCAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATAACGATCGATAAGCTAATAATAACCTTTTTCAGTAACATGCACAg                             >  2:1552247/1‑100 (MQ=255)
                                                                   cAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGa                            >  2:2694188/1‑100 (MQ=255)
                                                                   cAATAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGa                            >  2:3750896/1‑100 (MQ=255)
                                                                     aTAGCTCAATAATAGAATAAAACGATCGATATTTATTTTATCTTTCGTTTATATCGATCGATAAGCTAATAATATCCTTTGTCAGTAACATGCACAGATa                          <  2:1886036/100‑1 (MQ=255)
                                                                     aTAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATa                          <  2:2425260/100‑1 (MQ=255)
                                                                     aTAGCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATa                          <  2:2101058/100‑1 (MQ=255)
                                                                        gCTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGt                       >  1:3460987/1‑100 (MQ=255)
                                                                         cTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTa                      >  2:952245/1‑100 (MQ=255)
                                                                         cTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTa                      >  2:1564600/1‑100 (MQ=255)
                                                                         cTCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTa                      >  1:182046/1‑100 (MQ=255)
                                                                          tCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTAc                     <  2:235292/100‑1 (MQ=255)
                                                                          tCAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTAc                     <  2:1913912/100‑1 (MQ=255)
                                                                           cAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACa                    <  1:1564612/100‑1 (MQ=255)
                                                                           cAATAATAGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACa                    >  1:3516989/1‑100 (MQ=255)
                                                                              taataGAATAAGACGATCGATATCTATTTTATCGATAGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACa                    >  2:3386569/1‑97 (MQ=255)
                                                                              taataGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGaa                 >  2:3820618/1‑100 (MQ=255)
                                                                               aataGATTAAACGGAGCGATACCTTTTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGaaa                <  2:1490557/100‑1 (MQ=255)
                                                                               aataGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGaaa                <  1:1723136/100‑1 (MQ=255)
                                                                                ataGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAg               <  2:3515149/100‑1 (MQ=255)
                                                                                  aGAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGAACAGATACGTACAGAAAGAc             >  2:3211333/1‑100 (MQ=255)
                                                                                   gAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGGTACGTACAGAAAGACa            <  2:3868990/100‑1 (MQ=255)
                                                                                   gAATAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACa            >  2:2320129/1‑100 (MQ=255)
                                                                                    aaTAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACAt           <  1:3770220/100‑1 (MQ=255)
                                                                                      tAAAACGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTc         >  2:1382591/1‑100 (MQ=255)
                                                                                         aaCGATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAgg      <  2:3917022/100‑1 (MQ=255)
                                                                                           cgatcgatATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGa    >  1:329477/1‑100 (MQ=255)
                                                                                            gATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGaa   <  1:2846987/100‑1 (MQ=255)
                                                                                            gATCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGaa   <  2:1551178/100‑1 (MQ=255)
                                                                                             aTCGATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGaac  >  1:1272752/1‑100 (MQ=255)
                                                                                                |                                                                                                
GATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAAC  >  NC_000913/2071225‑2071417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: