Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,090,124 C→G 15.3% intergenic (+78/‑68) hisL → / → hisG his operon leader peptide/ATP phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,090,1240CG15.3% 332.6 / 43.2 121intergenic (+78/‑68)hisL/hisGhis operon leader peptide/ATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (51/51);  new base G (8/11);  total (59/62)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 128 total aligned reads displayed.

TCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTAT  >  NC_000913/2090025‑2090223
                                                                                                   |                                                                                                   
tcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATc                                                                                                     <  1:2815457/100‑1 (MQ=255)
tcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCACGGAAGATc                                                                                                     >  2:1054320/1‑100 (MQ=255)
 catcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATcn                                                                                                    <  1:3657526/100‑2 (MQ=255)
 catcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATc                                                                                                     >  2:3955704/1‑99 (MQ=255)
 catcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCa                                                                                                    <  1:1726075/100‑1 (MQ=255)
     accatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCtt                                                                                                >  2:1206750/1‑100 (MQ=255)
       catcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc                                                                                              >  2:2655862/1‑100 (MQ=255)
        atcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTACATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCg                                                                                             >  2:1804814/1‑100 (MQ=255)
         tcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                                                                            <  1:1669501/100‑1 (MQ=255)
          catcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                                                           <  2:2193590/100‑1 (MQ=255)
          catcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                                                           <  1:1722239/100‑1 (MQ=255)
           atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCgggg                                                                                          <  1:1123896/100‑1 (MQ=39)
           atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCgggg                                                                                          <  2:2161787/100‑1 (MQ=39)
           atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCgggg                                                                                          <  1:816423/100‑1 (MQ=39)
           atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                          >  1:2010431/1‑100 (MQ=255)
           atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                          <  1:3392557/100‑1 (MQ=255)
           atcCGGACTAGTCTTTCAGGCGATGTGTTCTGGAAGACATTCAGATCCTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCgggg                                                                                          <  2:1660848/100‑1 (MQ=38)
            tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCggggg                                                                                         <  1:194858/100‑1 (MQ=38)
            tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCggggg                                                                                         <  1:2714516/100‑1 (MQ=38)
            tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCggggg                                                                                         <  1:1484117/100‑1 (MQ=38)
            tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                                                                         >  1:521635/1‑100 (MQ=255)
            tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATAAAAAAGCCCCCGGAAGGTGATCTTCCggggg                                                                                         <  1:1539644/100‑1 (MQ=37)
            tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTAGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                                                                            >  2:175352/1‑97 (MQ=255)
             ccTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGc                                                                                        <  1:247843/100‑1 (MQ=255)
              cTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCt                                                                                       <  1:364119/100‑1 (MQ=255)
              cTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCt                                                                                       <  1:363932/100‑1 (MQ=255)
                  cTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttttt                                                                                   >  2:4004269/1‑100 (MQ=255)
                  cTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttttt                                                                                   >  2:2530039/1‑100 (MQ=255)
                   tAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttttt                                                                                   <  2:3531293/99‑1 (MQ=255)
                    aGTCTTTCAGGTGATGTGTGCTGGAAGACAGTCTGATCTTTCAGTGGTTTTTGTACGCATTAGAATGCCCCCGGAAGGTGATCTTCCGGGGGCtttctca                                                                                 <  2:1012984/100‑5 (MQ=16)
                    aGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTa                                                                                 >  1:2212510/1‑100 (MQ=255)
                     gTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAt                                                                                >  2:2818992/1‑100 (MQ=255)
                     gTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAt                                                                                <  1:3969185/100‑1 (MQ=255)
                     gTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAt                                                                                <  1:1246257/100‑1 (MQ=255)
                     gTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAt                                                                                <  1:1972272/100‑1 (MQ=255)
                          tCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                                                                           <  1:1412015/100‑1 (MQ=255)
                            aGGCGATGTGTGCTGGAAGACATTCAGATCTACCGGTGGTGCAAGAACGAATGAGAAAGCCAGCGCAACATGTACTGCCGGAGGCTTATTTATTg                                                                              >  2:3065204/1‑95 (MQ=255)
                             ggCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGt                                                                        <  1:2085446/100‑1 (MQ=255)
                                gATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGa                                                                     <  2:1686500/100‑1 (MQ=255)
                                  tgtgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATa                                                                   <  1:3495710/100‑1 (MQ=255)
                                  tgtgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATa                                                                   <  1:2029390/100‑1 (MQ=255)
                                   gtgtgCTGGAAGACATGCAGATCGTCCGGTGGTGCTTGAACGCATGACACAGCCCCCGGATGATCAGGCTCGGGAAGCTTTTTTATTgttcgttt                                                                       <  2:2527971/95‑8 (MQ=255)
                                    tgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAAc                                                                 <  2:3155528/100‑1 (MQ=255)
                                     gtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGGGCGGTTGATAACg                                                                >  1:2541063/1‑100 (MQ=255)
                                      tgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACgg                                                               >  1:217631/1‑100 (MQ=255)
                                       gCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGt                                                              >  2:16652/1‑100 (MQ=255)
                                       gCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGt                                                              >  1:3888844/1‑100 (MQ=255)
                                        cTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGtt                                                             >  2:596768/1‑100 (MQ=255)
                                          ggAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCa                                                           >  1:558269/1‑100 (MQ=255)
                                           gAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTTCGCGGTTGATAACGGTTCAg                                                          <  2:2528675/100‑1 (MQ=255)
                                           gAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAg                                                          <  1:2832483/100‑1 (MQ=255)
                                              gACATTCAGATCTTCCTGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACa                                                       <  2:2418368/100‑1 (MQ=255)
                                                  ttCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGtt                                                   >  2:350199/1‑100 (MQ=255)
                                                   tCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGttt                                                  >  1:3487302/1‑100 (MQ=255)
                                                    cAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTa                                                 >  2:2323716/1‑100 (MQ=255)
                                                    cAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTa                                                 >  2:4134831/1‑100 (MQ=255)
                                                    cAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTa                                                 >  2:895673/1‑100 (MQ=255)
                                                     aGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaa                                                <  1:139984/100‑1 (MQ=255)
                                                     aGAACTTCCAGTAGTGCATGAACGCATGAGACAGCCCCCGGCCGATCAAATTCCGAGGGCATGTGTATTCCGCGCATGATAACGGTTCAGACAgacgt                                                  >  2:3800599/1‑94 (MQ=255)
                                                      aaTCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaaa                                               >  2:1260042/2‑100 (MQ=255)
                                                       aTCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAag                                              <  2:3810441/100‑1 (MQ=255)
                                                       aTCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAag                                              <  1:2022189/100‑1 (MQ=255)
                                                       aTCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAag                                              <  1:2323729/100‑1 (MQ=255)
                                                            ccAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGaa                                         <  2:1094660/100‑1 (MQ=255)
                                                            ccAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGaa                                         <  1:3048658/100‑1 (MQ=255)
                                                                 ggtgCATGAACGCATGAGAGAGCCCCCGGAAGATCACCTGACGGGGGCATTTTGATGGCGGGGTTGATGACGGTTCAGACAGTTTTACAGAGGAATAACa                                    >  2:3168602/1‑100 (MQ=255)
                                                                    gCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACaaaa                                 <  2:81251/100‑1 (MQ=255)
                                                                       tGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATga                              >  1:26837/1‑100 (MQ=255)
                                                                       tGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATga                              <  1:86320/100‑1 (MQ=255)
                                                                         aaCGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgaca                            <  1:2762191/100‑1 (MQ=255)
                                                                         aaCGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgaca                            >  1:936943/1‑100 (MQ=255)
                                                                           gcaataaaaaaGCCCCCGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTCCAGACAGGTTTAAAGAGGAATAACAAAATgacaga                          >  2:1047535/9‑100 (MQ=21)
                                                                           cGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacaga                          >  1:3118154/1‑100 (MQ=255)
                                                                            gCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacagac                         >  1:109064/1‑100 (MQ=255)
                                                                            gCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacagac                         <  1:3518246/100‑1 (MQ=255)
                                                                                gagaAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaaca                     >  1:1647799/1‑100 (MQ=255)
                                                                                    aaGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCg                 <  2:137032/100‑1 (MQ=255)
                                                                                    aaGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCg                 >  2:1910871/1‑100 (MQ=255)
                                                                                     aGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGt                <  2:2010418/100‑1 (MQ=255)
                                                                                      tctcccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAATGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGtt               <  2:1248725/97‑1 (MQ=38)
                                                                                      gCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCTCGGTTGATAACGGATCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCg                 >  2:4099103/1‑98 (MQ=255)
                                                                                      gCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGtt               <  1:1695717/100‑1 (MQ=255)
                                                                                       cccccGGAAGATCACCTTCCGGGGGCTTCTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGttt              >  1:499114/1‑100 (MQ=255)
                                                                                        ccccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTa             >  1:1837517/1‑100 (MQ=39)
                                                                                        ccccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGCATAACAAAATGACAGACAACACTCGttt              >  1:4035131/1‑99 (MQ=255)
                                                                                        ccccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTa             >  2:229484/1‑100 (MQ=255)
                                                                                        ccccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTa             >  1:1244998/1‑100 (MQ=255)
                                                                                        ccccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTa             >  1:1707335/1‑100 (MQ=255)
                                                                                         cccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAAGACTCGTTTAc            >  2:2721107/1‑100 (MQ=38)
                                                                                         cccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTAc            >  1:2167408/1‑100 (MQ=255)
                                                                                         cccGGAAGATCACCTTCCGGGGGCTTTTTGATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTAc            >  2:3467276/1‑100 (MQ=255)
                                                                                          ccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACg           >  2:2032681/1‑100 (MQ=255)
                                                                                           cGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCATACATGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGc          <  2:2366815/100‑1 (MQ=255)
                                                                                             gAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGCATAACAAAATGACAGACAACACTCGTTTACGCAt        >  2:3246292/1‑100 (MQ=255)
                                                                                              aaGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATa       <  1:3246312/100‑1 (MQ=255)
                                                                                                gATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGc     <  1:939688/100‑1 (MQ=255)
                                                                                                 aTCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCt    >  1:4085162/1‑100 (MQ=255)
                                                                                                  tCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTa   <  1:1311992/100‑1 (MQ=255)
                                                                                                   cACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTAt  <  2:370220/100‑1 (MQ=255)
                                                                                                   cACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTAt  >  2:3801906/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTAT  >  NC_000913/2090025‑2090223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: