Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,090,126 C→T 15.0% intergenic (+80/‑66) hisL → / → hisG his operon leader peptide/ATP phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,090,1260CT15.0% 300.8 / 37.0 123intergenic (+80/‑66)hisL/hisGhis operon leader peptide/ATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (51/51);  new base T (7/11);  total (60/63)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

Only 100 of 128 total aligned reads displayed.

TCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGC  >  NC_000913/2090028‑2090225
                                                                                                  |                                                                                                   
  accatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCtt                                                                                                  >  2:1206750/1‑100 (MQ=255)
    catcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc                                                                                                >  2:2655862/1‑100 (MQ=255)
     atcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTACATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCg                                                                                               >  2:1804814/1‑100 (MQ=255)
      tcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                                                                              <  1:1669501/100‑1 (MQ=255)
       catcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                                                             <  2:2193590/100‑1 (MQ=255)
       catcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                                                             <  1:1722239/100‑1 (MQ=255)
        atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCgggg                                                                                            <  1:1123896/100‑1 (MQ=39)
        atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCgggg                                                                                            <  2:2161787/100‑1 (MQ=39)
        atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCgggg                                                                                            <  1:816423/100‑1 (MQ=39)
        atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                            <  1:3392557/100‑1 (MQ=255)
        atcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                            >  1:2010431/1‑100 (MQ=255)
        atcCGGACTAGTCTTTCAGGCGATGTGTTCTGGAAGACATTCAGATCCTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCgggg                                                                                            <  2:1660848/100‑1 (MQ=38)
         tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCggggg                                                                                           <  1:1484117/100‑1 (MQ=38)
         tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCggggg                                                                                           <  1:194858/100‑1 (MQ=38)
         tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCggggg                                                                                           <  1:2714516/100‑1 (MQ=38)
         tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                                                                           >  1:521635/1‑100 (MQ=255)
         tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATAAAAAAGCCCCCGGAAGGTGATCTTCCggggg                                                                                           <  1:1539644/100‑1 (MQ=37)
         tcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTAGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                                                                              >  2:175352/1‑97 (MQ=255)
          ccTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGc                                                                                          <  1:247843/100‑1 (MQ=255)
           cTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCt                                                                                         <  1:363932/100‑1 (MQ=255)
           cTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCt                                                                                         <  1:364119/100‑1 (MQ=255)
               cTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttttt                                                                                     >  2:2530039/1‑100 (MQ=255)
               cTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttttt                                                                                     >  2:4004269/1‑100 (MQ=255)
                tAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttttt                                                                                     <  2:3531293/99‑1 (MQ=255)
                 aGTCTTTCAGGTGATGTGTGCTGGAAGACAGTCTGATCTTTCAGTGGTTTTTGTACGCATTAGAATGCCCCCGGAAGGTGATCTTCCGGGGGCtttctca                                                                                   <  2:1012984/100‑5 (MQ=16)
                 aGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTa                                                                                   >  1:2212510/1‑100 (MQ=255)
                  gTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAt                                                                                  >  2:2818992/1‑100 (MQ=255)
                  gTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAt                                                                                  <  1:1972272/100‑1 (MQ=255)
                  gTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAt                                                                                  <  1:1246257/100‑1 (MQ=255)
                  gTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAt                                                                                  <  1:3969185/100‑1 (MQ=255)
                      ttCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCTGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                                                              <  2:567047/100‑1 (MQ=255)
                       tCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                                                                             <  1:1412015/100‑1 (MQ=255)
                         aGGCGATGTGTGCTGGAAGACATTCAGATCTACCGGTGGTGCAAGAACGAATGAGAAAGCCAGCGCAACATGTACTGCCGGAGGCTTATTTATTg                                                                                >  2:3065204/1‑95 (MQ=255)
                          ggCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGt                                                                          <  1:2085446/100‑1 (MQ=255)
                             gATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGa                                                                       <  2:1686500/100‑1 (MQ=255)
                               tgtgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATa                                                                     <  1:2029390/100‑1 (MQ=255)
                               tgtgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATa                                                                     <  1:3495710/100‑1 (MQ=255)
                                gtgtgCTGGAAGACATGCAGATCGTCCGGTGGTGCTTGAACGCATGACACAGCCCCCGGATGATCAGGCTCGGGAAGCTTTTTTATTgttcgttt                                                                         <  2:2527971/95‑8 (MQ=255)
                                 tgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAAc                                                                   <  2:3155528/100‑1 (MQ=255)
                                  gtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGGGCGGTTGATAACg                                                                  >  1:2541063/1‑100 (MQ=255)
                                   tgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACgg                                                                 >  1:217631/1‑100 (MQ=255)
                                    gCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGt                                                                >  2:16652/1‑100 (MQ=255)
                                     cTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGtt                                                               >  2:596768/1‑100 (MQ=255)
                                       ggAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCa                                                             >  1:558269/1‑100 (MQ=255)
                                       ggAAGACATTCAGATCTTCCAGTGGTACATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCa                                                             <  1:1804827/100‑1 (MQ=255)
                                        gAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTTCGCGGTTGATAACGGTTCAg                                                            <  2:2528675/100‑1 (MQ=255)
                                        gAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAg                                                            <  1:2832483/100‑1 (MQ=255)
                                           gACATTCAGATCTTCCTGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACa                                                         <  2:2418368/100‑1 (MQ=255)
                                               ttCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGtt                                                     >  2:350199/1‑100 (MQ=255)
                                                tCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGttt                                                    >  1:3487302/1‑100 (MQ=255)
                                                 cAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTa                                                   >  2:2323716/1‑100 (MQ=255)
                                                 cAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTa                                                   >  2:4134831/1‑100 (MQ=255)
                                                 cAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTa                                                   >  2:895673/1‑100 (MQ=255)
                                                  aGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaa                                                  <  1:139984/100‑1 (MQ=255)
                                                  aGAACTTCCAGTAGTGCATGAACGCATGAGACAGCCCCCGGCCGATCAAATTCCGAGGGCATGTGTATTCCGCGCATGATAACGGTTCAGACAgacgt                                                    >  2:3800599/1‑94 (MQ=255)
                                                   aaTCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaaa                                                 >  2:1260042/2‑100 (MQ=255)
                                                    aTCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAag                                                <  2:3810441/100‑1 (MQ=255)
                                                    aTCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAag                                                <  1:2022189/100‑1 (MQ=255)
                                                    aTCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAag                                                <  1:2323729/100‑1 (MQ=255)
                                                         ccAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGaa                                           <  2:1094660/100‑1 (MQ=255)
                                                              ggtgCATGAACGCATGAGAGAGCCCCCGGAAGATCACCTGACGGGGGCATTTTGATGGCGGGGTTGATGACGGTTCAGACAGTTTTACAGAGGAATAACa                                      >  2:3168602/1‑100 (MQ=255)
                                                               gtgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACaa                                     >  1:2987485/1‑100 (MQ=255)
                                                                 gCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACaaaa                                   <  2:81251/100‑1 (MQ=255)
                                                                   aTGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAATGGTTCAGACAGGTTTAAAGAGGAATAACAAAATg                                 <  1:471618/100‑1 (MQ=255)
                                                                    tGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATga                                <  1:86320/100‑1 (MQ=255)
                                                                    tGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATga                                >  1:26837/1‑100 (MQ=255)
                                                                      aaCGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgaca                              >  1:936943/1‑100 (MQ=255)
                                                                      aaCGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgaca                              <  1:2762191/100‑1 (MQ=255)
                                                                        gcaataaaaaaGCCCCCGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTCCAGACAGGTTTAAAGAGGAATAACAAAATgacaga                            >  2:1047535/9‑100 (MQ=21)
                                                                        cGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacaga                            >  1:3118154/1‑100 (MQ=255)
                                                                         gCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacagac                           >  1:109064/1‑100 (MQ=255)
                                                                         gCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacagac                           <  1:3518246/100‑1 (MQ=255)
                                                                          cATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacagaca                          <  1:990742/100‑1 (MQ=255)
                                                                            tGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaac                        >  1:3322593/1‑100 (MQ=255)
                                                                             gagaAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaaca                       >  1:1647799/1‑100 (MQ=255)
                                                                                 aaGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCg                   >  2:1910871/1‑100 (MQ=255)
                                                                                 aaGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCg                   <  2:137032/100‑1 (MQ=255)
                                                                                  aGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGt                  <  2:2010418/100‑1 (MQ=255)
                                                                                   tctcccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAATGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGtt                 <  2:1248725/97‑1 (MQ=38)
                                                                                   gCCCCCGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGtt                 >  1:37056/1‑100 (MQ=255)
                                                                                   gCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCTCGGTTGATAACGGATCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCg                   >  2:4099103/1‑98 (MQ=255)
                                                                                   gCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGtt                 <  1:1695717/100‑1 (MQ=255)
                                                                                     ccccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGCATAACAAAATGACAGACAACACTCGttt                >  1:4035131/1‑99 (MQ=255)
                                                                                     ccccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTa               >  2:229484/1‑100 (MQ=255)
                                                                                     ccccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTa               >  1:1244998/1‑100 (MQ=255)
                                                                                     ccccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTa               >  1:1707335/1‑100 (MQ=255)
                                                                                      cccGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAAGACTCGTTTAc              >  2:2721107/1‑100 (MQ=38)
                                                                                      cccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTAc              >  1:2167408/1‑100 (MQ=255)
                                                                                      cccGGAAGATCACCTTCCGGGGGCTTTTTGATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTAc              >  2:3467276/1‑100 (MQ=255)
                                                                                       ccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACg             >  2:2032681/1‑100 (MQ=255)
                                                                                        cGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCATACATGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGc            <  2:2366815/100‑1 (MQ=255)
                                                                                          gAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGCATAACAAAATGACAGACAACACTCGTTTACGCAt          >  2:3246292/1‑100 (MQ=255)
                                                                                           aaGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATa         <  1:3246312/100‑1 (MQ=255)
                                                                                              aTCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCt      >  1:4085162/1‑100 (MQ=255)
                                                                                               tCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTa     <  1:1311992/100‑1 (MQ=255)
                                                                                                cACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTAt    <  2:370220/100‑1 (MQ=255)
                                                                                                cACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTAt    >  2:3801906/1‑100 (MQ=255)
                                                                                                 aCCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATg   >  1:813240/1‑100 (MQ=255)
                                                                                                  ccTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGc  >  1:641903/1‑100 (MQ=255)
                                                                                                  ccTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGc  >  1:1954953/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
TCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGC  >  NC_000913/2090028‑2090225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: