Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,159,354 C→T 24.9% R323R (CGC→CGT mdtC → multidrug efflux system, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,159,3540CT24.9% 230.5 / 77.1 132R323R (CGC→CGTmdtCmultidrug efflux system, subunit C
Reads supporting (aligned to +/- strand):  ref base C (47/52);  new base T (12/21);  total (59/73)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01

Only 100 of 133 total aligned reads displayed.

AGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGG  >  NC_000913/2159255‑2159452
                                                                                                   |                                                                                                  
aGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGc                                                                                                    >  2:1244509/1‑100 (MQ=255)
 gCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCt                                                                                                   <  1:476762/100‑1 (MQ=255)
  ccAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTc                                                                                                  >  2:3932101/1‑100 (MQ=255)
  ccAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTc                                                                                                  >  2:1200283/1‑100 (MQ=255)
   cAATATTATCCAGGCGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAGTTGCCCAGGATCGTTcc                                                                                                 <  2:2373866/100‑1 (MQ=255)
   cAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTcc                                                                                                 <  2:1071565/100‑1 (MQ=255)
    aaTATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTccc                                                                                                <  1:4028635/100‑1 (MQ=255)
    aaTATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTccc                                                                                                <  1:1991478/100‑1 (MQ=255)
      tattatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTccccc                                                                                              <  1:2102524/100‑1 (MQ=255)
       attatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCcca                                                                                             >  2:2920519/1‑100 (MQ=255)
       agtacccAGACTGTTGGCAGACTCCGGGCAAACTTTCCGGAGTTGGAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTccccc                                                                                              <  2:4065326/94‑1 (MQ=255)
        ttatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCTCAGGATCGCTCCCccac                                                                                            <  2:925930/100‑1 (MQ=255)
        ttatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCccac                                                                                            <  1:4008853/100‑1 (MQ=255)
        ttatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCccac                                                                                            <  1:196531/100‑1 (MQ=255)
         tatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGTTCTGCAAATTGCCCAGGTTCGCTCCCccacc                                                                                           <  1:2781022/100‑1 (MQ=255)
         tatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGCTCGCTCCCccacc                                                                                           <  1:2780548/100‑1 (MQ=255)
         tatCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCccacc                                                                                           <  1:2592878/100‑1 (MQ=255)
          atCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCccacca                                                                                          <  1:2697219/100‑1 (MQ=255)
               gACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTcgc                                                                                     >  1:3224078/1‑100 (MQ=255)
                aCGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTcgcg                                                                                    <  2:1916648/100‑1 (MQ=255)
                aCGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTcgcg                                                                                    <  2:1022275/100‑1 (MQ=255)
                aCGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTcgcg                                                                                    <  2:3224058/100‑1 (MQ=255)
                aCGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTcgcg                                                                                    <  1:1326118/100‑1 (MQ=255)
                 cGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCgcgc                                                                                   <  2:2130179/100‑1 (MQ=255)
                 cGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCgcgc                                                                                   <  1:3510701/100‑1 (MQ=255)
                 cGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCgcgc                                                                                   >  1:1624419/1‑100 (MQ=255)
                  ggTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGcc                                                                                  <  1:2043803/100‑1 (MQ=255)
                  ggTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGcc                                                                                  <  1:3327618/100‑1 (MQ=255)
                      gACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGc                                                                              >  2:3607155/1‑100 (MQ=255)
                      gACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGc                                                                              >  1:1208203/1‑100 (MQ=255)
                       aCAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCt                                                                             >  1:8598/1‑100 (MQ=255)
                       aCAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCt                                                                             >  2:4115416/1‑100 (MQ=255)
                        cAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTg                                                                            <  1:2770256/100‑1 (MQ=255)
                         aGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTgg                                                                           <  2:3011083/100‑1 (MQ=255)
                          gCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGga                                                                          <  1:43859/100‑1 (MQ=255)
                           cATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGgaa                                                                         <  1:3518792/100‑1 (MQ=255)
                            aTCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGgaag                                                                        <  2:1184617/100‑1 (MQ=255)
                            aTCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGgaag                                                                        <  1:2074441/100‑1 (MQ=255)
                            aTCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGgaag                                                                        <  2:2952023/100‑1 (MQ=255)
                            aTCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGgaag                                                                        <  2:3607204/100‑1 (MQ=255)
                               cGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGaagaag                                                                     <  1:1225190/100‑1 (MQ=255)
                                  gCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCg                                                                  >  1:2061023/1‑100 (MQ=255)
                                   cAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGa                                                                 >  2:2987914/1‑100 (MQ=255)
                                     aaaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGc                                                               >  1:2686634/1‑100 (MQ=255)
                                     aaaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGc                                                               >  2:543972/1‑100 (MQ=255)
                                      aaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCa                                                              <  1:1650984/100‑1 (MQ=255)
                                         tACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAAc                                                           <  2:69027/100‑1 (MQ=255)
                                         tACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAAc                                                           >  1:516389/1‑100 (MQ=255)
                                           ccGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGc                                                         >  1:207036/1‑100 (MQ=255)
                                           ccGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGc                                                         <  1:985284/100‑1 (MQ=255)
                                             ggAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTg                                                       >  2:4104243/1‑100 (MQ=255)
                                              gAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGa                                                      <  2:3534285/100‑1 (MQ=255)
                                              gAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGa                                                      <  1:504379/100‑1 (MQ=255)
                                               aGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGAt                                                     <  1:2474209/100‑1 (MQ=255)
                                                gTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGAtt                                                    <  1:1031618/100‑1 (MQ=255)
                                                gTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGAtt                                                    <  2:558308/100‑1 (MQ=255)
                                                gTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGAtt                                                    >  1:3201195/1‑100 (MQ=255)
                                                gTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGAtt                                                    <  2:2380345/100‑1 (MQ=255)
                                                  tGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAt                                                  >  1:2900731/1‑100 (MQ=255)
                                                    cAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAtct                                                >  2:908673/1‑100 (MQ=255)
                                                    cAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTAtct                                                >  2:3873727/1‑100 (MQ=255)
                                                      ggAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCg                                              >  2:2596895/1‑100 (MQ=255)
                                                      ggAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCg                                              <  1:1398894/100‑1 (MQ=255)
                                                       gAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCgg                                             <  1:728693/100‑1 (MQ=255)
                                                            cATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGgcg                                        >  1:1679537/1‑100 (MQ=255)
                                                             aTTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGgcgc                                       >  1:1680541/1‑100 (MQ=255)
                                                              ttCCGGCGGCGATTGATCTGCAAATTGCCGAGGTTCGTTCCCCAACCGTTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCt                                      <  2:1226228/100‑1 (MQ=255)
                                                                 cggcggCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGt                                   >  1:3225123/1‑100 (MQ=255)
                                                                 cggcggCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGt                                   <  1:2286813/100‑1 (MQ=255)
                                                                   gcggcgATTGAACTGCAAATTGCCCAGGTTCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTg                                  <  2:2062621/99‑1 (MQ=255)
                                                                    cggcgATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCggtggt                                   >  2:2306748/1‑97 (MQ=255)
                                                                       cgATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCt                             <  1:3343108/100‑1 (MQ=255)
                                                                       cgATTGATCTGCAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCt                             <  1:1015457/100‑1 (MQ=255)
                                                                        gATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCtg                            >  1:3803239/1‑100 (MQ=255)
                                                                          ttGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCtggt                          <  1:3482930/100‑1 (MQ=255)
                                                                            gATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCtggtgg                        >  1:2528050/1‑100 (MQ=255)
                                                                            gATCGGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCtggtgg                        <  2:438317/100‑1 (MQ=255)
                                                                             aTCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTggtggt                       >  1:417605/1‑100 (MQ=255)
                                                                              tCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGgtggtg                      >  1:3137031/1‑100 (MQ=255)
                                                                               cTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGgtgt                     >  1:729546/1‑100 (MQ=255)
                                                                                tGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTggtggt                       >  2:590282/1‑97 (MQ=255)
                                                                                tGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGtt                    >  1:2928258/1‑100 (MQ=255)
                                                                                tGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGtt                    >  1:3882035/1‑100 (MQ=255)
                                                                                 gCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGttt                   >  1:858491/1‑100 (MQ=255)
                                                                                 gCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGttt                   <  2:3882010/100‑1 (MQ=255)
                                                                                 gCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCCGGTGAtatt                             >  2:1919836/1‑87 (MQ=255)
                                                                                  cAAATTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGtttt                  <  1:3952547/100‑1 (MQ=255)
                                                                                   aaaTTGCCCAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGttttt                 >  1:2137629/1‑100 (MQ=255)
                                                                                    aggtGCTCAGGGACGCTCCCCCGCCGGTCGTGTCTCGGTGGAAGAAGTCGAGCAGACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGttt                   <  2:789625/94‑1 (MQ=255)
                                                                                    aaTTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTa                <  1:804841/100‑1 (MQ=255)
                                                                                      ttGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTAtt              <  1:595328/100‑1 (MQ=255)
                                                                                       tGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTc             >  1:1616545/1‑100 (MQ=255)
                                                                                        gCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTAttgc            >  2:2500229/1‑98 (MQ=255)
                                                                                        gCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTcc            >  1:3291845/1‑100 (MQ=255)
                                                                                        gCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTcc            <  1:3873752/100‑1 (MQ=255)
                                                                                         cccAGGATCGTTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCCGCGGTGACTCTGAAGGTGATATTATTCCt           >  2:660422/1‑100 (MQ=255)
                                                                                          ccAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTg          >  1:1491642/1‑100 (MQ=255)
                                                                                          ccAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTg          <  1:1604364/100‑1 (MQ=255)
                                                                                              gggcGCTCCGCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTg          <  2:1624407/93‑1 (MQ=255)
                                                                                                  gCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCggg  <  1:2688779/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
AGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGCGCTGGTGATTCTGGTGGTGTTTTTATTCCTGCGCTCGGG  >  NC_000913/2159255‑2159452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: