Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,161,946 A→G 26.4% A161A (GCA→GCG iceT → putative citrate/iron‑citrate/zinc‑citrate efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,161,9460AG26.4% 209.1 / 89.4 140A161A (GCA→GCGiceTputative citrate/iron‑citrate/zinc‑citrate efflux transporter
Reads supporting (aligned to +/- strand):  ref base A (55/47);  new base G (14/23);  total (69/71)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.17e-01

Only 100 of 144 total aligned reads displayed.

GCGCGAGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGACGCGGCGC  >  NC_000913/2161847‑2162045
                                                                                                   |                                                                                                   
gcgcgAGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCa                                                                                                     <  2:99361/100‑1 (MQ=255)
   cgAGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCg                                                                                                  >  1:4082845/1‑100 (MQ=255)
    gAGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGcgtcgt                                                                                                 >  2:3652264/1‑100 (MQ=255)
     aGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTg                                                                                                >  2:734431/1‑100 (MQ=255)
       cgatgtatGGCGGCGATGACCGTTGTCACGTTACCCGTTCAGGTCGGTCCGGTGCTCGGTCCGGCGCGCGGCGGTCTGCTGGTGGAGTACGCATCGTGGc                                                                                              <  2:2883339/95‑1 (MQ=255)
         atatatGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCAc                                                                                            <  1:3074074/100‑1 (MQ=255)
          tatatGGCGGCGATGACCTTTGTCGCGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACt                                                                                           <  2:3098588/100‑1 (MQ=255)
          tatatGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTTCGGCGCTCGGTGGTCTGCTGGTGGAGTATGCATCGTGGCatt                                                                                           <  1:2789981/100‑3 (MQ=255)
          tatatGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACt                                                                                           >  2:3908042/1‑100 (MQ=255)
          tatatGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACt                                                                                           <  1:1268961/100‑1 (MQ=255)
           agatGGCGGGGGTGGCCTGTGCCTCGTTGCCAGGGCGGGGCGGTGCGCGGCTCGGCCCGGCGCTCGACAGTCTGATGTTGGAGTACGCATCGTGGCAc                                                                                            <  2:2348791/96‑1 (MQ=255)
               ggcggcGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATc                                                                                      <  1:3777377/100‑1 (MQ=255)
                   gcgATGACCTTTGTCACGTTCCCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCtttt                                                                                  <  2:860926/100‑1 (MQ=255)
                   gcgATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGGGTACGCATCGTGGCACTGGATCtttt                                                                                  <  2:37104/100‑1 (MQ=255)
                   gcgATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCtttt                                                                                  <  2:1665294/100‑1 (MQ=255)
                    cgATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCttttt                                                                                 <  1:2498551/100‑1 (MQ=255)
                    cgATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCttttt                                                                                 <  1:2794468/100‑1 (MQ=255)
                    cgATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCttttt                                                                                 <  1:101494/100‑1 (MQ=255)
                    cgATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCttttt                                                                                 <  1:2398900/100‑1 (MQ=255)
                    cgATGACCTTTGTCACGTTACCCGGGCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCttttt                                                                                 >  1:1854793/1‑100 (MQ=255)
                    ccaTGGCCTTTGTCACGTTCTCTGGTTAGTTCGGTCCGCTGCGCGGTCCGGGGCTCGGCCGTCTGCTGGTGGTTTACGCATCGTGGCACTGGATCttttt                                                                                 <  2:354850/98‑1 (MQ=255)
                     gATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTg                                                                                <  2:2626509/100‑1 (MQ=255)
                         aCCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGCATCTTTTTGATCa                                                                            <  1:2810670/100‑1 (MQ=255)
                          ccTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCaa                                                                           >  1:863896/1‑100 (MQ=255)
                          ccTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCaa                                                                           >  1:538123/1‑100 (MQ=255)
                          ccTTTGTCACGTTACCCGGGCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTg                                                                                >  2:3748184/1‑95 (MQ=255)
                           cTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAAc                                                                          <  1:3742657/100‑1 (MQ=255)
                               gttaCGTTATCCGGTCAGGTCGGTCCGCTGCTGGGTCCGGCGCTGGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGAGCAACAttg                                                                      <  2:3502118/97‑2 (MQ=255)
                               gTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTc                                                                      >  1:362773/1‑100 (MQ=255)
                               gTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTc                                                                      >  2:1838967/1‑100 (MQ=255)
                                 cACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGGGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCg                                                                    >  2:1138397/1‑100 (MQ=255)
                                   cGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGt                                                                  >  1:2748155/1‑100 (MQ=255)
                                        cccGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTgggg                                                              >  2:3282238/1‑99 (MQ=255)
                                        cccGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTgggg                                                              >  2:3497076/1‑99 (MQ=255)
                                        cccGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGa                                                             >  2:3389932/1‑100 (MQ=255)
                                        cccGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGa                                                             >  1:3474692/1‑100 (MQ=255)
                                          cGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGAtt                                                           >  2:2878935/1‑100 (MQ=255)
                                           ggTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTa                                                          >  1:213569/1‑100 (MQ=255)
                                                gtttggttCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTAGGCATCGGGGCACTGGATCGTTTTGATCAACATTCCGGTGGGGATTATCgg                                                      <  2:1798495/95‑1 (MQ=255)
                                                ggtcggtgCGCAGCCTGGTCCGGCGCTCGGCGGTCTGCTGGCGGAGTACGCATCGCGTCCCTGGAGCTTGTTGATTAATCTTCCTGTgggggctagtgtt                                                     >  2:2176785/1‑91 (MQ=255)
                                                ggtcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGt                                                     >  2:2618682/1‑100 (MQ=255)
                                                 gtcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTg                                                    <  1:1647977/100‑1 (MQ=255)
                                                 gtcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTg                                                    <  1:96402/100‑1 (MQ=255)
                                                 gtcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTg                                                    >  2:1602587/1‑100 (MQ=255)
                                                 gtcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTg                                                    >  1:3088540/1‑100 (MQ=255)
                                                   cggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCTGTGGGGATTATCGGTGCg                                                  <  2:1618455/100‑1 (MQ=255)
                                                     gtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGAt                                                >  1:681197/1‑100 (MQ=255)
                                                     gtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGAt                                                >  1:3514110/1‑100 (MQ=255)
                                                      tcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATc                                               <  1:683639/100‑1 (MQ=255)
                                                      tcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATc                                               >  1:3382856/1‑100 (MQ=255)
                                                      tcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATc                                               <  1:456977/100‑1 (MQ=255)
                                                        cGCTGCTCGGTCCGGCGCTCGGCGGTTTGCTGGTGGAGTACGCAGCGTGGCACTGGATCTTTTTGATCAATATTCCGGTGGGGATTATCGGTGCGATCg                                              <  2:124410/99‑1 (MQ=255)
                                                        cGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGc                                             >  2:3520287/1‑100 (MQ=255)
                                                        cGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGc                                             <  1:3393586/100‑1 (MQ=255)
                                                         gctgctCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGcc                                            <  1:724896/100‑1 (MQ=255)
                                                         gctgctCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGagatcg                                            >  1:3880168/1‑95 (MQ=255)
                                                             ctCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCcaca                                         <  2:3194915/99‑1 (MQ=255)
                                                             ctCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACAt                                        <  1:4002240/100‑1 (MQ=255)
                                                              tCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACAtt                                       >  1:3677245/1‑100 (MQ=255)
                                                               cGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTg                                      >  1:2980494/1‑100 (MQ=255)
                                                                  ttgggCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTg                                   <  2:493675/97‑1 (MQ=255)
                                                                  tCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTg                                   <  1:3664037/100‑1 (MQ=255)
                                                                   ccGGCTCTCGGCGGTGTGCTGGGGGAGTCTGGCCCGGGGCGCCGGATCTGTTTGATCAACATTCCTGTGGGGATGATCGGTGCGATCGCCACATTGCTGt                                  <  2:158737/100‑1 (MQ=255)
                                                                   ccGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGt                                  <  1:2609744/100‑1 (MQ=255)
                                                                    cGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGtt                                 <  1:515089/100‑1 (MQ=255)
                                                                    cGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGtt                                 >  1:593029/1‑100 (MQ=255)
                                                                     ggCGCTCGGCGGTCTGCTGGTGGGGTACGCGTGGTGGCACTGGATCTTTTTGAGCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGtt                                 <  2:2171390/99‑1 (MQ=255)
                                                                     ggCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTa                                <  2:251915/100‑1 (MQ=255)
                                                                     ggCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTa                                >  2:3412728/1‑100 (MQ=255)
                                                                      gcgcTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTaa                               <  2:1469385/100‑1 (MQ=255)
                                                                       cgcTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAAt                              <  1:3087446/100‑1 (MQ=255)
                                                                       cgcTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAAt                              >  1:3284984/1‑100 (MQ=255)
                                                                       cgcTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAAt                              <  1:3497098/100‑1 (MQ=255)
                                                                        gcTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATg                             >  2:414890/1‑100 (MQ=255)
                                                                         cTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGc                            >  2:1200464/1‑100 (MQ=255)
                                                                             gcggTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGaa                        >  1:1323532/1‑100 (MQ=255)
                                                                              cggTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAAc                       <  2:1267568/100‑1 (MQ=255)
                                                                              cggTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAAc                       <  1:39279/100‑1 (MQ=255)
                                                                               ggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACt                      <  2:3750699/100‑1 (MQ=255)
                                                                                gTCTGTTTGTGGAGTATGCATCGTGGCACTGGTTCTTTTTGATCAACATTCTGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTa                     <  2:1041632/100‑1 (MQ=255)
                                                                                  ctgctgGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTaca                   <  1:2616483/100‑1 (MQ=255)
                                                                                   tgctgGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGCTCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTac                    >  2:2448437/1‑98 (MQ=255)
                                                                                   tgctgGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTacac                  <  1:3537325/100‑1 (MQ=255)
                                                                                       ggtggAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATg              >  2:1383822/1‑100 (MQ=255)
                                                                                       ggtggAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATg              >  1:775134/1‑100 (MQ=255)
                                                                                        gtggAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATAGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCCTGc             >  2:2663375/1‑100 (MQ=255)
                                                                                         tggAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCa            <  2:681191/100‑1 (MQ=255)
                                                                                         tggAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCa            >  1:2153993/1‑100 (MQ=255)
                                                                                         tggAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCa            <  2:2046865/100‑1 (MQ=255)
                                                                                           gAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATGCCGGTGGGGCTTATCGGTGCGATCGCCACATTGCTGTTAATCCCGAACTACACCATGcn            <  2:1966191/98‑2 (MQ=255)
                                                                                            ngTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGAc         >  1:189444/2‑100 (MQ=255)
                                                                                            aGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGAc         >  2:2975877/1‑100 (MQ=255)
                                                                                            aGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGAc         <  1:2776861/100‑1 (MQ=255)
                                                                                            aGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGAc         >  1:1558242/1‑100 (MQ=255)
                                                                                              tACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGAcgc       >  2:472990/1‑100 (MQ=255)
                                                                                                 gCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGACgcggc    <  1:20617/100‑1 (MQ=255)
                                                                                                  cATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACCCCATGCAGAcgccgc    >  2:932364/1‑99 (MQ=255)
                                                                                                  cATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGACgcggcg   >  1:731226/1‑100 (MQ=255)
                                                                                                   aTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATTCCGAACTACACCATGCAGACGCGgcgc  >  2:3506327/1‑100 (MQ=255)
                                                                                                   aTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGACGCGgcgc  >  1:3605287/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GCGCGAGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGACGCGGCGC  >  NC_000913/2161847‑2162045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: