Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,183,656 T→A 54.1% intergenic (‑5/+60) yegX ← / ← thiD putative family 25 glycosyl hydrolase/hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,183,6560TA54.1% 45.7 / 202.7 150intergenic (‑5/+60)yegX/thiDputative family 25 glycosyl hydrolase/hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase
Reads supporting (aligned to +/- strand):  ref base T (30/39);  new base A (34/47);  total (64/86)
Fisher's exact test for biased strand distribution p-value = 8.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

GTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGACCA  >  NC_000913/2183560‑2183749
                                                                                                |                                                                                             
 tCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCAt                                                                                           >  1:732108/1‑100 (MQ=255)
  cTGACGCCGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAACTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGttt                                                                                              <  1:3660273/96‑1 (MQ=255)
    gACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATccc                                                                                        <  1:3510514/100‑1 (MQ=255)
    gACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATccc                                                                                        <  1:3988581/100‑1 (MQ=255)
    gACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATccc                                                                                        <  1:2758107/100‑1 (MQ=255)
    gACGCGGATAAAATGCAAGGATGGAAATTAGCCCAAGAGCGCATATTAGAGGGGTGAGTTTTTTTCGGCTGGTAATTCGTAGCTGCATTGGtt                                                                                               <  2:2565230/93‑1 (MQ=255)
     aCGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCt                                                                                       <  1:1107057/100‑1 (MQ=255)
      cgcgGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTg                                                                                      >  2:1124514/1‑100 (MQ=255)
      cgcgGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTg                                                                                      <  1:2220989/100‑1 (MQ=255)
       gcgGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGa                                                                                     <  2:3280431/100‑1 (MQ=255)
        cgGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGATATTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGa                                                                                     >  1:706663/1‑99 (MQ=255)
         ggATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAAt                                                                                   >  2:2379423/1‑100 (MQ=255)
          gATAAATTGCAAGGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAACTCTTAGCTGCATTGGTATCATCCCTGAATg                                                                                  <  2:2710784/100‑1 (MQ=255)
           aTAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGt                                                                                 >  1:790212/1‑100 (MQ=255)
            tAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTc                                                                                <  2:1529705/100‑1 (MQ=255)
            tAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTc                                                                                >  1:2062450/1‑100 (MQ=255)
              aaTTGCAACGAGGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAg                                                                              <  2:884399/100‑1 (MQ=255)
               aTTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGt                                                                             <  1:3067042/100‑1 (MQ=255)
               aTTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAACTGCCTTGGTATCATCCCTGAATGTCAGt                                                                             >  2:740182/1‑100 (MQ=255)
                 tGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGc                                                                           >  1:3331482/1‑100 (MQ=255)
                  gCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGcc                                                                          <  2:553029/100‑1 (MQ=255)
                   atctgATGGAAATTAGCCCAAGAGCGCATAATAGAGAGTTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCa                                                                         <  1:762046/96‑1 (MQ=255)
                    aaCGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCaa                                                                        >  1:3829909/1‑100 (MQ=255)
                      cGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAg                                                                      >  1:1522042/1‑100 (MQ=255)
                      cGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAg                                                                      <  1:938497/100‑1 (MQ=255)
                       gATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTTGTTTCATCCCTGAATGTCAGTGCCaagat                                                                     >  2:762040/1‑97 (MQ=255)
                        aTGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGc                                                                    >  1:1402469/1‑100 (MQ=255)
                        aTGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGc                                                                    <  1:2648336/100‑1 (MQ=255)
                        aTGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGc                                                                    <  1:189963/100‑1 (MQ=255)
                           gAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGa                                                                 >  1:804424/1‑100 (MQ=255)
                               ttAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAAt                                                             <  1:2606087/100‑1 (MQ=255)
                                 aGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATaa                                                           <  2:3765596/100‑1 (MQ=255)
                                  ggccAAGAGCGCATAATAGTCAGGTGAGTTTGTGTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAAc                                                          <  2:1803211/98‑1 (MQ=255)
                                  gCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAAc                                                          >  2:2087151/1‑100 (MQ=255)
                                  gCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGCTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATaa                                                           >  2:2253644/1‑99 (MQ=255)
                                  gCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGCTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAAc                                                          >  2:2252560/1‑100 (MQ=255)
                                   cccAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAAcc                                                         >  2:723415/1‑100 (MQ=255)
                                   cacAAGAGCGCATAATAGATAAGTTAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAAc                                                          <  1:406283/97‑1 (MQ=255)
                                    ccAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCa                                                        <  1:2379437/100‑1 (MQ=255)
                                    ccAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCa                                                        <  2:2122190/100‑1 (MQ=255)
                                    ccAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCa                                                        >  1:466701/1‑100 (MQ=255)
                                     cAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCaa                                                       >  2:3959283/1‑100 (MQ=255)
                                        gagCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAagc                                                    <  1:731646/100‑1 (MQ=255)
                                         agCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAagca                                                   <  2:2032086/100‑1 (MQ=255)
                                         agCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAagca                                                   <  2:2680121/100‑1 (MQ=255)
                                            gcATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCt                                                <  2:1429622/100‑1 (MQ=255)
                                            gcATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCt                                                <  1:142824/100‑1 (MQ=255)
                                              ataataGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCtat                                              <  1:1622511/100‑1 (MQ=255)
                                               taataGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCtata                                             >  1:4027752/1‑100 (MQ=255)
                                                aataGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAg                                            <  2:3096272/100‑1 (MQ=255)
                                                   agagagGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTAc                                         >  1:1582487/1‑100 (MQ=255)
                                                    gagagGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACg                                        >  1:60381/1‑100 (MQ=255)
                                                        ggTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGc                                    <  2:2603696/100‑1 (MQ=255)
                                                        ggTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGc                                    <  1:393624/100‑1 (MQ=255)
                                                        ggTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGc                                    >  1:62416/1‑100 (MQ=255)
                                                        ggTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGc                                    <  1:3163427/100‑1 (MQ=255)
                                                        ggTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGc                                    >  2:1407844/1‑100 (MQ=255)
                                                        ggTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGc                                    <  1:667330/100‑1 (MQ=255)
                                                        ggTGAGTTTTCTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGCCTTACAATAACCAAAGCAGCTATAGTACGGTGc                                    <  2:2715968/100‑1 (MQ=255)
                                                         gTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCt                                   >  1:524161/1‑100 (MQ=255)
                                                           gAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCTCTGAATGTCAGTGCCAAAGGCTGACAATAATCAAAGCAGCTATAGTACGGTGCTca                                 <  2:241687/100‑1 (MQ=255)
                                                            aGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTcac                                >  1:3682358/1‑100 (MQ=255)
                                                             gTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTcacc                               <  1:1569411/100‑1 (MQ=255)
                                                             gTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTcacc                               <  1:851878/100‑1 (MQ=255)
                                                             gTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTcacc                               >  1:3632689/1‑100 (MQ=255)
                                                             gTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTcacc                               >  1:1207242/1‑100 (MQ=255)
                                                             gTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTcacc                               <  2:3611338/100‑1 (MQ=255)
                                                              tttttttCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTcacca                              >  2:591344/1‑100 (MQ=255)
                                                               ttttttCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTcacca                              <  1:3937578/99‑1 (MQ=255)
                                                                tttttCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCaccacc                            <  1:1624209/100‑1 (MQ=255)
                                                                tttttCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCaccacc                            <  1:2302595/100‑1 (MQ=255)
                                                                 ttttCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCAccacca                           <  2:3331462/100‑1 (MQ=255)
                                                                  tttCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAg                          >  1:948206/1‑100 (MQ=255)
                                                                   ttCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAgg                         <  2:1492496/100‑1 (MQ=255)
                                                                   ttCGACTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAgg                         <  2:1858741/100‑1 (MQ=255)
                                                                     cGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCg                       <  1:3217471/100‑1 (MQ=255)
                                                                      gACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGt                      >  1:860396/1‑100 (MQ=255)
                                                                       aCTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTg                     >  2:3353446/1‑100 (MQ=255)
                                                                       aCTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGtn                     <  2:3103325/100‑2 (MQ=255)
                                                                        cTGGTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTgg                    >  2:1062328/1‑100 (MQ=255)
                                                                          ggTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGaa                  >  2:3548303/1‑100 (MQ=255)
                                                                          ggTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGaa                  >  1:1275017/1‑100 (MQ=255)
                                                                          ggTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGa                   >  1:372977/1‑99 (MQ=255)
                                                                           gTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAg                 <  1:3057222/100‑1 (MQ=255)
                                                                           gTAATTCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAg                 >  2:3544834/1‑100 (MQ=255)
                                                                            tAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGATATAGTACGGTGCGCACCACCAGGCGTGGAAgt                <  2:2741399/100‑1 (MQ=255)
                                                                              aTTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAgtgg              <  1:114642/100‑1 (MQ=255)
                                                                              aTTCTTAGCTGCATTGGTAGCATCCCTGAATGTCAGTGCCAAAGGCTGACAAGAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAgtgg              <  2:25702/100‑1 (MQ=255)
                                                                                tCTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAgtggtg            >  2:3855464/1‑100 (MQ=255)
                                                                                 cTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGa           >  1:2607717/1‑100 (MQ=255)
                                                                                 cTTAGCTGCATTGGTATCATGCCTGAATGTCAGTGCCAAAGGCTGGCAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGa           >  2:1220498/1‑100 (MQ=255)
                                                                                 cTTAGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGa           <  2:2810982/100‑1 (MQ=255)
                                                                                    aGCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAAcc        <  2:1796342/100‑1 (MQ=255)
                                                                                     gCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCg       <  2:1303698/100‑1 (MQ=255)
                                                                                     gCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCg       >  1:2446154/1‑100 (MQ=255)
                                                                                     gCTGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCg       <  1:2414745/100‑1 (MQ=255)
                                                                                      cTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTAACCACCAGGCGAGGAAGTGGTGAAcc        >  2:390713/1‑98 (MQ=255)
                                                                                       tGCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGa     <  2:1841463/100‑1 (MQ=255)
                                                                                        gCATTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGAc    <  1:3962939/100‑1 (MQ=255)
                                                                                          aTTGGTATCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGACCa  <  1:1606896/100‑1 (MQ=255)
                                                                                                |                                                                                             
GTCTGACGCGGATAAATTGCAACGATGGAAATTAGCCCAAGAGCGCATAATAGAGAGGTGAGTTTTTTTCGACTGGTAATTCTTAGCTGCATTGGTTTCATCCCTGAATGTCAGTGCCAAAGGCTGACAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGGTGAACCGGACCA  >  NC_000913/2183560‑2183749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: