Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,235,656 T→C 15.7% I131T (ATC→ACC)  preA → dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,235,6560TC15.7% 320.7 / 49.5 140I131T (ATC→ACC) preAdihydropyrimidine dehydrogenase, NADH‑dependent, subunit C
Reads supporting (aligned to +/- strand):  ref base T (53/65);  new base C (14/8);  total (67/73)
Fisher's exact test for biased strand distribution p-value = 1.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.98e-01

Only 100 of 140 total aligned reads displayed.

ACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGTGA  >  NC_000913/2235560‑2235754
                                                                                                |                                                                                                  
aCCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGa                                                                                                 <  1:1250530/100‑1 (MQ=255)
 cccGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGGCTGGTGCAAGAAGCTGGCGCGGATATGATCGAg                                                                                                >  2:1608822/1‑100 (MQ=255)
 cccGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAg                                                                                                <  2:435643/100‑1 (MQ=255)
 cccGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAg                                                                                                >  1:3107911/1‑100 (MQ=255)
 cccGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAg                                                                                                >  2:1818437/1‑100 (MQ=255)
 cccGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAg                                                                                                >  2:3684423/1‑100 (MQ=255)
  ccGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAgt                                                                                               >  2:1178084/1‑100 (MQ=255)
  ccGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAgt                                                                                               >  1:2593865/1‑100 (MQ=255)
   cGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAgtg                                                                                              <  2:3436585/100‑1 (MQ=255)
   cGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAgtg                                                                                              <  2:1562752/100‑1 (MQ=255)
       cAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAAc                                                                                          >  2:3928885/1‑100 (MQ=255)
        aaaGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAAc                                                                                          <  2:2903723/99‑1 (MQ=255)
         aaGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACtt                                                                                        >  2:2719616/1‑100 (MQ=255)
           gTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTtct                                                                                      <  1:3438293/100‑1 (MQ=255)
            tATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTtctc                                                                                     <  2:726358/100‑1 (MQ=255)
             aTTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTcc                                                                                    <  1:1531234/100‑1 (MQ=255)
             aTTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTcc                                                                                    <  1:1252591/100‑1 (MQ=255)
             aTTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTcc                                                                                    <  2:1032739/100‑1 (MQ=255)
             aTTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTcc                                                                                    <  1:873345/100‑1 (MQ=255)
                gATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTg                                                                                  >  1:3780391/1‑99 (MQ=255)
                gATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGt                                                                                 >  2:739840/1‑100 (MQ=255)
                gATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGt                                                                                 >  1:3947002/1‑100 (MQ=255)
                gATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGt                                                                                 >  1:1441479/1‑100 (MQ=255)
                 aTCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTc                                                                                <  2:1555993/100‑1 (MQ=255)
                  tCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTcc                                                                               <  1:2719632/100‑1 (MQ=255)
                   cGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCAGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCg                                                                              >  1:2430819/1‑100 (MQ=255)
                    gCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGc                                                                             >  1:639719/1‑100 (MQ=255)
                    gCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGc                                                                             >  2:659922/1‑100 (MQ=255)
                     cTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCa                                                                            <  2:3420290/100‑1 (MQ=255)
                     cTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCa                                                                            >  1:749948/1‑100 (MQ=255)
                     cTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCa                                                                            >  2:93770/1‑100 (MQ=255)
                        cGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAAt                                                                         <  2:3847169/100‑1 (MQ=255)
                        cGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAAt                                                                         <  2:3354105/100‑1 (MQ=255)
                        cGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAAt                                                                         >  2:1940595/1‑100 (MQ=255)
                        cGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAAt                                                                         <  1:3153572/100‑1 (MQ=255)
                            cATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACt                                                                     <  1:1141784/100‑1 (MQ=255)
                            cATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACt                                                                     >  1:2640535/1‑100 (MQ=255)
                            cATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACt                                                                     >  2:2277770/1‑100 (MQ=255)
                             aTGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACtt                                                                    >  1:3268584/1‑100 (MQ=255)
                               ggggggAAATGAGCAGCAATGGGAGTAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAGCTTCTCCTGTCCCCAATTGACTtc                                                                   <  2:2160935/99‑1 (MQ=255)
                                ggggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTtctc                                                                 >  2:2104550/1‑100 (MQ=255)
                                 gggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCa                                                                >  1:3919611/1‑100 (MQ=255)
                                 gggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCa                                                                >  1:3373432/1‑100 (MQ=255)
                                   gAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATg                                                              <  2:3069352/100‑1 (MQ=255)
                                     aaaTGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCg                                                            <  1:1488483/100‑1 (MQ=255)
                                      aaTGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGACGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCCTGc                                                             >  2:2372426/1‑98 (MQ=255)
                                      aaTGAGCAGCAATGGGAGGAGCTGGCGCGCATGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATAACTTCTCATGCGa                                                           >  2:785343/1‑100 (MQ=255)
                                       aTGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGAt                                                          <  1:3173265/100‑1 (MQ=255)
                                       aTGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGAt                                                          >  1:1104187/1‑100 (MQ=255)
                                        tGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATg                                                         <  2:106801/100‑1 (MQ=255)
                                             agcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAg                                                    <  2:3486708/100‑1 (MQ=255)
                                             agcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAg                                                    >  1:3485526/1‑100 (MQ=255)
                                              gcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTGCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGc                                                   <  2:1610610/100‑1 (MQ=255)
                                              gcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGc                                                   <  1:997903/100‑1 (MQ=255)
                                              gcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGc                                                   <  1:1428213/100‑1 (MQ=255)
                                              gcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATACGATCGGGTGTAACTTCTCCTGGCCGCAAATGACTTCTCATGCGATGGGTAg                                                    >  1:2154843/1‑99 (MQ=255)
                                               caATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCg                                                  <  1:2493009/100‑1 (MQ=255)
                                               caATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCg                                                  <  1:152474/100‑1 (MQ=255)
                                                aaTGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGa                                                 <  2:3600149/100‑1 (MQ=255)
                                                aaTGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGa                                                 <  2:1065803/100‑1 (MQ=255)
                                                 aTGGGAGGAGCCGGCGCGGCTGGTGCAAGAAGCTGGCGCGGATATGATCGCGTGTAAATTGTCCTGTCCGAAAATGACTTCTCATGCGATGGGTAGCg                                                  <  2:3100771/98‑1 (MQ=255)
                                                  tGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATg                                               <  1:4068354/100‑1 (MQ=255)
                                                   gggAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGt                                              >  2:3342909/1‑100 (MQ=255)
                                                   gggAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGt                                              >  1:2546479/1‑100 (MQ=255)
                                                    ggaggaGCTGGCGCACCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTc                                             <  1:3988477/100‑1 (MQ=255)
                                                     gaggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCg                                            >  1:3989783/1‑100 (MQ=255)
                                                     gaggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCg                                            <  1:63172/100‑1 (MQ=255)
                                                      aggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCgg                                           >  2:2241759/1‑100 (MQ=255)
                                                       ggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCggg                                          >  2:1617849/1‑100 (MQ=255)
                                                       ggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCggg                                          <  2:1236357/100‑1 (MQ=255)
                                                        gagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGc                                         <  1:3337586/100‑1 (MQ=255)
                                                            tGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAg                                     <  2:3608805/100‑1 (MQ=255)
                                                            tGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAg                                     <  2:698769/100‑1 (MQ=255)
                                                               ctcgcCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGccc                                  <  2:2018656/98‑1 (MQ=255)
                                                                 cgcCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCgg                                <  1:426887/100‑1 (MQ=255)
                                                                  gcCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGa                               <  2:730380/100‑1 (MQ=255)
                                                                  gcCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGa                               <  1:3838942/100‑1 (MQ=255)
                                                                   ccTGGTGCAAGATGCTGGCGCTGAGATGCTGGAGTGTAACTTGTCCCGTCCGCAGATGACTGCTCATGCGATGGGTAGCGATGTCGGGCAAAGCTCgggg                              <  2:3222773/100‑3 (MQ=255)
                                                                   ccTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAg                              <  1:787469/100‑1 (MQ=255)
                                                                     tGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCt                            <  1:1117603/100‑1 (MQ=255)
                                                                       gTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTgg                          <  2:2893568/100‑1 (MQ=255)
                                                                       gTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCGCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTgg                          <  2:2305779/100‑1 (MQ=255)
                                                                        tGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGt                         >  2:3675034/1‑100 (MQ=255)
                                                                        tGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGt                         <  2:2508933/100‑1 (MQ=255)
                                                                        tGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGt                         <  2:1385371/100‑1 (MQ=255)
                                                                           aagaagCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGa                      >  1:2155780/1‑100 (MQ=255)
                                                                            agaagCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGaa                     >  1:1719267/1‑100 (MQ=255)
                                                                            agaagCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGa                      >  2:786920/1‑99 (MQ=255)
                                                                                 cTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAata                <  1:2573488/100‑1 (MQ=255)
                                                                                  tGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAatat               <  1:2640895/100‑1 (MQ=255)
                                                                                  tGGCGCGGATATGACCGAGTGTAACGTCTCCTGTCCGCAAGTGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAatat               <  2:3980301/100‑1 (MQ=255)
                                                                                   ggCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATAtt              >  2:182075/1‑100 (MQ=255)
                                                                                   ggCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATAtt              >  1:650231/1‑100 (MQ=255)
                                                                                          atatGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGc       >  1:2470454/1‑100 (MQ=255)
                                                                                            atGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAg     <  2:2496627/100‑1 (MQ=255)
                                                                                             tGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGt    >  2:914380/1‑100 (MQ=255)
                                                                                             tGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGt    >  2:492776/1‑100 (MQ=255)
                                                                                               aTCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGTGa  >  2:703736/1‑100 (MQ=255)
                                                                                               aTCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGGAGAAAAATATTGTCGGGCAGTGa  >  1:1924590/1‑100 (MQ=255)
                                                                                               aTCGAGGGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGTGa  >  1:1930216/1‑100 (MQ=255)
                                                                                                |                                                                                                  
ACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAGTGA  >  NC_000913/2235560‑2235754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: