Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,239,199 T→C 52.1% N31D (AAC→GAC)  mglA ← methyl‑galactoside ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,239,1990TC52.1% 25.8 / 162.0 126N31D (AAC→GAC) mglAmethyl‑galactoside ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (37/23);  new base C (35/30);  total (73/53)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

TAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAATTTTA  >  NC_000913/2239102‑2239298
                                                                                                 |                                                                                                   
tAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCAt                                                                                                   >  1:3570866/1‑100 (MQ=255)
 aaaTACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATc                                                                                                  <  1:2949532/100‑1 (MQ=255)
     aCCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGt                                                                                              >  1:2148688/1‑100 (MQ=255)
      ccAAACAGGCATTTTAATAATGTCGCTTTTCCTGCAACGTTTTCCCACATTAATACATGGATAGAATGTGGCCGGACATTTAAAGTAACGCTATCAAGTg                                                                                             >  2:3889241/1‑100 (MQ=255)
      ccAAACAGGCATTTGAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAACGCATGGATGGAAGTTGGACGGACTTTTAATTTAACGTGATCAAGTg                                                                                             >  2:1011197/1‑100 (MQ=255)
        aaaCAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGcc                                                                                           <  2:3841456/100‑1 (MQ=255)
          aCAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCtt                                                                                         <  1:1250512/100‑1 (MQ=255)
           cAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTa                                                                                        >  1:3523986/1‑100 (MQ=255)
           cAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTa                                                                                        >  1:3318420/1‑100 (MQ=255)
             ggCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAac                                                                                      >  1:2995577/1‑100 (MQ=255)
              gCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAac                                                                                      <  2:3477697/99‑1 (MQ=255)
              gCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAaca                                                                                     <  1:2481667/100‑1 (MQ=255)
               cATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATTGATAGAATGTGGCCGGACTTTTAAATTAAGGTTATCAAGTGACTTaa                                                                                       >  2:516984/1‑97 (MQ=255)
               cATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAacac                                                                                    >  2:3112327/1‑100 (MQ=255)
               cATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAacac                                                                                    >  1:2052189/1‑100 (MQ=255)
               cATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAaca                                                                                     <  2:228545/99‑1 (MQ=255)
                   ttAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAgg                                                                                <  1:1052391/100‑1 (MQ=255)
                    taataaTCTCGATTTTCCTGCACAGTGTTACCCCTTTCCTGAATTGATAGACTGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCa                                                                                  <  2:3341984/97‑1 (MQ=255)
                     aataatGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGaa                                                                              >  2:1351396/1‑100 (MQ=255)
                     aataatGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGaa                                                                              <  2:1358455/100‑1 (MQ=255)
                      ataatGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGaaa                                                                             >  2:1310412/1‑100 (MQ=255)
                       taatGTCGATTTTCCTGCACCATTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAg                                                                            <  2:184067/100‑1 (MQ=255)
                          tGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGAc                                                                         >  1:1601750/1‑100 (MQ=255)
                              gATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACttgt                                                                     <  1:426164/100‑1 (MQ=255)
                              gATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACttgt                                                                     >  2:1226072/1‑100 (MQ=255)
                              gATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACt                                                                        <  2:142006/97‑1 (MQ=255)
                              gATGTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACttgt                                                                     <  2:801885/100‑1 (MQ=255)
                                  ttCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGAt                                                                 <  1:1174666/100‑1 (MQ=255)
                                   tCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATa                                                                >  2:1525673/1‑100 (MQ=255)
                                   tCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATa                                                                >  1:3717505/1‑100 (MQ=255)
                                   tCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATa                                                                <  1:453532/100‑1 (MQ=255)
                                     cTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATAcc                                                              >  2:277363/1‑100 (MQ=255)
                                     cTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATAcc                                                              >  2:592411/1‑100 (MQ=255)
                                     cTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATAcc                                                              <  1:404309/100‑1 (MQ=255)
                                      tGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCg                                                             >  1:2855365/1‑100 (MQ=255)
                                       gCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGc                                                            >  1:3868786/1‑100 (MQ=255)
                                       gCACCGTTTTCCCCCATTAATGAATGGATAAAATGTGGCAGGACTTTTAAAGTAACGTCATCAAGTGCCTTAACACCCGGAAAGGACTAGTTGATACCGc                                                            >  2:1738230/1‑100 (MQ=255)
                                        cACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCt                                                           <  1:3905878/100‑1 (MQ=255)
                                          ccGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCa                                                         <  1:1662854/100‑1 (MQ=255)
                                          ccGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCa                                                         >  2:2636923/1‑100 (MQ=255)
                                              tttCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTc                                                     >  2:4032427/1‑100 (MQ=255)
                                                tCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCca                                                   >  2:715885/1‑100 (MQ=255)
                                                tCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCca                                                   <  1:894086/100‑1 (MQ=255)
                                                 cccccATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCcaa                                                  >  1:1633178/1‑100 (MQ=255)
                                                  ccccATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCcaac                                                 >  1:3368714/1‑100 (MQ=255)
                                                  ccccATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCcaac                                                 >  1:168649/1‑100 (MQ=255)
                                                  ccccATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCcaac                                                 <  2:2300443/100‑1 (MQ=255)
                                                  ccccATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCcaac                                                 <  1:3368128/100‑1 (MQ=255)
                                                  ccccATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCcaac                                                 >  1:614822/1‑100 (MQ=255)
                                                  ccccATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCcaac                                                 <  1:1328933/100‑1 (MQ=255)
                                                   cccATTAATGCATGGAGAGAATGTGGCCGGACTTTTAAATGTACGTTAGCAAGTGCCTTGACACCAGGAATGGACTGGTTGATACCGCTCATGTCcatca                                                >  1:829132/1‑100 (MQ=255)
                                                      aTTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAg                                              <  2:1395495/99‑1 (MQ=255)
                                                       ttAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCATGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTa                                            <  2:2014860/100‑1 (MQ=255)
                                                       ttAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTa                                            <  2:1542981/100‑1 (MQ=255)
                                                         aaTGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTAtt                                          <  2:3481216/100‑1 (MQ=255)
                                                         aaTGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTAtt                                          >  2:2676613/1‑100 (MQ=255)
                                                          atgcagttaTAGAATGCGGCCGTAGTTTTAAAATATCGGTATCAAGGGCCTGAACCCCAGGAAAGGACTTGTTGATACCGCTCATTTCcaacaa                                               <  2:3700043/86‑1 (MQ=255)
                                                            gCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTccc                                       >  1:690066/1‑100 (MQ=255)
                                                            gCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTcc                                        >  1:2769174/1‑99 (MQ=255)
                                                             cATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTcccc                                      >  1:1839638/1‑100 (MQ=255)
                                                             cATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTccc                                       <  2:3570391/99‑1 (MQ=255)
                                                               tGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCgg                                    <  1:353908/100‑1 (MQ=255)
                                                                 gATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCggag                                  >  1:2439041/1‑100 (MQ=255)
                                                                 gATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCggag                                  >  1:624716/1‑100 (MQ=255)
                                                                    aGAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGAc                               >  2:2288433/1‑100 (MQ=255)
                                                                     gAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACg                              >  2:1803393/1‑100 (MQ=255)
                                                                     gAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACg                              >  2:1913158/1‑100 (MQ=255)
                                                                     gAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACg                              >  1:2955908/1‑100 (MQ=255)
                                                                     gAATGTGGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACg                              <  2:2130460/100‑1 (MQ=255)
                                                                      aaTGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACgg                             <  2:1601738/100‑1 (MQ=255)
                                                                       aTGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGa                            <  1:1362268/100‑1 (MQ=255)
                                                                          tgGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTc                         >  2:3345988/1‑100 (MQ=255)
                                                                          tgGCCGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTc                         <  2:143936/100‑1 (MQ=255)
                                                                            gCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGt                       >  2:1820815/1‑100 (MQ=255)
                                                                             ccGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGtt                      >  1:382562/1‑100 (MQ=255)
                                                                             ccGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTACAACAAGTATTCCCCGGTGGACGGAGTcat                       >  1:730530/1‑97 (MQ=255)
                                                                              cGGACTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTg                     <  2:155352/100‑1 (MQ=255)
                                                                                gACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAg                   <  1:489287/100‑1 (MQ=255)
                                                                                 aCTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGc                  <  2:161572/100‑1 (MQ=255)
                                                                                 aCTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGc                  <  1:1556288/100‑1 (MQ=255)
                                                                                  cTTTTAAATTAACGTCATCAAGTTCCTTTACACCAGCACAGCACTTGTTGATACCGCTCATTTCCAACAAGTCTTCCCCGGAGGACGGAGTCTTTGAGCt                 >  2:1832630/1‑100 (MQ=255)
                                                                                  cTTTTAAATTAACGTCATCAAGTGCCTTAACACCATGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCt                 <  1:3802435/100‑1 (MQ=255)
                                                                                  cTTTTAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCt                 >  2:1255149/1‑100 (MQ=255)
                                                                                   ttttAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTg                >  2:2842417/1‑100 (MQ=255)
                                                                                   ttttAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGTACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCATTGAGCTg                >  2:4004606/1‑100 (MQ=255)
                                                                                   ttttAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTg                <  2:3772412/100‑1 (MQ=255)
                                                                                     ttAAATTAACTTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGAc              <  1:306869/100‑1 (MQ=255)
                                                                                     ttAAATTAACGTTATCAAGTGCCTTAACACCAGGTAAGGAATTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGAc              >  2:3380916/1‑100 (MQ=255)
                                                                                     ttAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGAc              <  1:171578/100‑1 (MQ=255)
                                                                                     ttAAATTAACGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGAc              <  1:3611008/100‑1 (MQ=255)
                                                                                      tAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGAcc             <  2:3401011/100‑1 (MQ=255)
                                                                                        aaTTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCat           >  2:1068342/1‑100 (MQ=255)
                                                                                        aaTTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCat           >  2:3103237/1‑100 (MQ=255)
                                                                                        aaTTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCat           >  2:708051/1‑100 (MQ=255)
                                                                                            aaCGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGAACGGAGTCGTTGAGCTGACCATATaa       >  1:23287/1‑100 (MQ=255)
                                                                                             aCGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAAt      >  1:1236260/1‑100 (MQ=255)
                                                                                              cGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAAtt     >  2:1962056/1‑100 (MQ=255)
                                                                                              cGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAAtt     >  2:2430129/1‑100 (MQ=255)
                                                                                              cGTCATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCACGGAGGACGGAGTCGTTGAGCTGACCATATAAtt     <  2:2999671/100‑1 (MQ=255)
                                                                                                 tATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAATTTTa  <  1:2044097/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
TAAATACCAAACAGGCATTTTAATAATGTCGATTTTCCTGCACCGTTTTCCCCCATTAATGCATGGATAGAATGTGGCCGGACTTTTAAATTAACGTTATCAAGTGCCTTAACACCAGGAAAGGACTTGTTGATACCGCTCATTTCCAACAAGTATTCCCCGGAGGACGGAGTCGTTGAGCTGACCATATAATTTTA  >  NC_000913/2239102‑2239298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: