Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,255,991 A→T 16.6% K213* (AAA→TAA) ‡ yeiL → putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,255,9910AT16.6% 431.4 / 70.0 173K213* (AAA→TAA) yeiLputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (93/51);  new base T (16/13);  total (109/64)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

Only 100 of 180 total aligned reads displayed.

TTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGC  >  NC_000913/2255892‑2256090
                                                                                                   |                                                                                                   
 tcattcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATaa                                                                                                    <  2:3866962/100‑1 (MQ=255)
  cattcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATaaa                                                                                                   >  2:2622141/1‑100 (MQ=255)
   attcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAAt                                                                                                  <  1:1407186/100‑1 (MQ=255)
   atgcaCGACGGTTTATTAATTGAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAATCAGTTGTCAAGACTGGCGCTGGAGATGGACCCGGAGAAGaaa                                                                                                   <  2:1439320/96‑1 (MQ=255)
          acgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCgg                                                                                           <  2:673040/100‑1 (MQ=255)
           cgGTTTATTAATTAAAAGCAAGAAAGGGTATCTGATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCggg                                                                                          <  2:1157408/100‑1 (MQ=255)
           cgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCggg                                                                                          <  2:3621008/100‑1 (MQ=255)
           cgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCggg                                                                                          <  1:3406412/100‑1 (MQ=255)
             gTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCgggtc                                                                                        <  1:3281174/100‑3 (MQ=255)
              tttATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACACGGAGAATAAATTCTCCGGgatg                                                                                       >  2:1908755/1‑100 (MQ=255)
                 attaattaAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGatgatg                                                                                    >  2:3420563/1‑100 (MQ=255)
                 attaattaAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGatgatg                                                                                    >  2:3013661/1‑100 (MQ=255)
                  ttaattaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGc                                                                                   >  1:1523962/1‑100 (MQ=255)
                  ttaattaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGc                                                                                   <  1:2861180/100‑1 (MQ=255)
                    aattaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAg                                                                                 >  1:606324/1‑100 (MQ=255)
                    aattaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAg                                                                                 <  1:713723/100‑1 (MQ=255)
                     attaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGt                                                                                >  2:2453044/1‑100 (MQ=255)
                     attaaAAGCAAGAAAGGGTAGCTCATTAAAAAAAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACAGGAAGAATAAATTCTACGGGATGAGGCAGt                                                                                >  2:4013770/1‑100 (MQ=255)
                      ttaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTa                                                                               >  2:1126887/1‑100 (MQ=255)
                        aaaaGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGAGCTGGAGATCGACCCAGAGAATAAGTTCTCCGGGATGATGCAGTaaa                                                                             >  2:2789056/1‑100 (MQ=255)
                         aaaGCAAGAAAGGGTATCTCATTGAAAACAGAAAGCAGTTGTCAGGAATGGCGCTGGAGATGGACCAGGAGAATAAATTCTCCGGGCTGATGCAGTaaaa                                                                            >  2:1445418/1‑100 (MQ=255)
                         aaaGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTaaaa                                                                            >  1:210799/1‑100 (MQ=255)
                           aGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAat                                                                          >  2:1556175/1‑100 (MQ=255)
                                gAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTAttt                                                                     <  2:4132804/100‑1 (MQ=255)
                                  aaGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTc                                                                   <  2:181919/100‑1 (MQ=255)
                                  aaGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTc                                                                   <  2:1342840/100‑1 (MQ=255)
                                  aaGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTc                                                                   >  1:3041938/1‑100 (MQ=255)
                                   ttccgaTCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCa                                                                  <  1:2269174/95‑1 (MQ=255)
                                   aGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCa                                                                  <  1:2874899/100‑1 (MQ=255)
                                   aGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCa                                                                  >  1:202304/1‑100 (MQ=255)
                                    gggTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCaa                                                                 >  1:844792/1‑100 (MQ=255)
                                    gggTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATAGACCCGGAGAGTAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCaa                                                                 >  2:2242270/1‑100 (MQ=255)
                                     ggTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAAt                                                                <  2:1318015/100‑1 (MQ=255)
                                     ggTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAAt                                                                <  2:1398923/100‑1 (MQ=255)
                                       tATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAg                                                              >  1:603223/1‑100 (MQ=255)
                                        aTCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAgc                                                             >  1:2499130/1‑100 (MQ=255)
                                           tcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGcgcg                                                          >  2:3160996/1‑100 (MQ=255)
                                            cATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGcgcg                                                          >  2:4054048/1‑99 (MQ=255)
                                            cATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGa                                                         >  2:2067944/1‑100 (MQ=255)
                                            cATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCGGGAGGTGGACCCGGAGGATAAATTCTCCGGGGTGATGCGGGGAAAGTGATTTTCAGTAGCGCGa                                                         >  1:3292643/1‑100 (MQ=255)
                                             aTTAAAAACAGAAGGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGAt                                                        >  2:2548425/1‑100 (MQ=255)
                                             aTTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGAt                                                        >  2:1042245/1‑100 (MQ=255)
                                             aTTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGAt                                                        >  1:518612/1‑100 (MQ=255)
                                              ttAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGAtt                                                       <  1:3161015/100‑1 (MQ=255)
                                                 aaaaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGcc                                                    >  1:3914681/1‑100 (MQ=255)
                                                 aaaaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGcc                                                    >  2:2769030/1‑100 (MQ=255)
                                                  aaaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCg                                                   <  1:2833229/100‑1 (MQ=255)
                                                    aCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGa                                                 >  1:411914/1‑100 (MQ=255)
                                                     cAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGAt                                                <  1:3013680/100‑1 (MQ=255)
                                                      aGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATg                                               <  1:223800/100‑1 (MQ=255)
                                                      aGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTGCAATAGCGCGATTGCCGGATg                                               >  1:1837789/1‑100 (MQ=255)
                                                      aGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACACGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATg                                               <  2:2022195/100‑1 (MQ=255)
                                                       gAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGc                                              <  2:1668943/100‑1 (MQ=255)
                                                       gAAAGCAGTGGTCAGGACTGGCGCTTGAGATGGACCCGGAGAATAACTTCTTCGGGATGATGCAGGAAGAGTGATTTTCAATAGCGCGATTGCCGGATGc                                              >  2:2042061/1‑100 (MQ=255)
                                                            cAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGc                                         >  1:2579361/1‑100 (MQ=255)
                                                            cAGTTGTCAGGAATGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGc                                         >  2:1370199/1‑100 (MQ=255)
                                                              gTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCtt                                       >  1:1277861/1‑100 (MQ=255)
                                                              gTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCtt                                       >  1:3869353/1‑100 (MQ=255)
                                                              gTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCtt                                       >  2:750386/1‑100 (MQ=255)
                                                              gTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCtt                                       >  2:1188806/1‑100 (MQ=255)
                                                              gTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCtt                                       >  2:2311248/1‑100 (MQ=255)
                                                               ttGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTa                                      <  1:3337848/100‑1 (MQ=255)
                                                                 ggcAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGGTGCGGTAAAACTTATTTTGAATAGCGCGATTGCCGGATGCGACGCTTa                                      >  2:3380417/3‑98 (MQ=255)
                                                                  tCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATa                                   >  1:1383772/1‑100 (MQ=255)
                                                                  tCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATa                                   <  2:1800845/100‑1 (MQ=255)
                                                                      gACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGtt                               >  1:2012214/1‑100 (MQ=255)
                                                                       aCTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGttt                              <  1:1043944/100‑1 (MQ=255)
                                                                       aCTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGttt                              <  1:3773218/100‑1 (MQ=255)
                                                                       aCTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGttt                              <  1:1789025/100‑1 (MQ=255)
                                                                        cTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGtttt                             >  2:967852/1‑100 (MQ=255)
                                                                        cTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGtttt                             >  2:80247/1‑100 (MQ=255)
                                                                        cTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGtttt                             >  2:2999570/1‑100 (MQ=255)
                                                                          ggCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTAt                           >  1:1878149/1‑100 (MQ=255)
                                                                           gcgcTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATc                          <  1:2769046/100‑1 (MQ=255)
                                                                             gcTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCg                        >  2:675688/1‑100 (MQ=255)
                                                                               tGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGt                      >  1:1095930/1‑100 (MQ=255)
                                                                                ggAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTc                     >  2:3186784/1‑100 (MQ=255)
                                                                                ggAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTc                     >  2:623870/1‑100 (MQ=255)
                                                                                 gagaTGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCt                    >  1:610657/1‑100 (MQ=255)
                                                                                     tGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACaa                >  1:3340114/1‑100 (MQ=255)
                                                                                     tGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACaa                >  2:4091925/1‑100 (MQ=255)
                                                                                      ggACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACaaa               >  1:1701589/1‑100 (MQ=255)
                                                                                      catcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACaaa               >  1:1020726/4‑100 (MQ=255)
                                                                                        aCCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATc             <  2:3751958/100‑1 (MQ=255)
                                                                                         cccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAAtag            >  2:2506640/1‑98 (MQ=255)
                                                                                         cccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCg            >  1:3082438/1‑100 (MQ=255)
                                                                                         cccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCg            >  1:2041980/1‑100 (MQ=255)
                                                                                         cccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCg            >  1:164589/1‑100 (MQ=255)
                                                                                          ccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGa           >  2:1503549/1‑100 (MQ=255)
                                                                                          ccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGa           >  2:1937488/1‑100 (MQ=255)
                                                                                          ccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGa           >  1:3595851/1‑100 (MQ=255)
                                                                                          ccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGa           >  1:1693256/1‑100 (MQ=255)
                                                                                             gagaATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGc         <  2:1626093/99‑1 (MQ=255)
                                                                                             gagaATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCa        <  2:233744/100‑1 (MQ=255)
                                                                                             gagaATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCa        <  1:675694/100‑1 (MQ=255)
                                                                                                aaTAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTa     <  1:1303243/100‑1 (MQ=255)
                                                                                                aaTAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTa     <  1:3454680/100‑1 (MQ=255)
                                                                                                  tAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACg   >  2:568756/1‑100 (MQ=255)
                                                                                                  tAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACg   >  1:2927784/1‑100 (MQ=255)
                                                                                                   aaaTTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGc  <  1:1556187/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGC  >  NC_000913/2255892‑2256090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: