Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,278,298 C→T 100% intergenic (‑61/+272) yejG ← / ← bcr uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,278,2980CT97.2% 403.6 / ‑0.2 124intergenic (‑61/+272)yejG/bcruncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base T (65/55);  total (67/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

GCAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACG  >  NC_000913/2278201‑2278392
                                                                                                 |                                                                                              
gCAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaa                                                                                              <  1:1923482/100‑1 (MQ=255)
gCAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATcaa                                                                                              <  1:153812/100‑1 (MQ=255)
 cAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTGCATCAATTTTAACACATCATtaac                                                                                             >  1:742628/1‑100 (MQ=255)
   gTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaacaa                                                                                           >  2:3636110/1‑100 (MQ=255)
     cGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATc                                                                                         <  2:223673/100‑1 (MQ=255)
      gacggACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAATCACATACACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATcc                                                                                        <  2:3710347/97‑1 (MQ=255)
      gATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATcc                                                                                        >  1:3070998/1‑100 (MQ=255)
       aTGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCg                                                                                       >  1:372779/1‑100 (MQ=255)
       aTGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCg                                                                                       >  1:3008430/1‑100 (MQ=255)
        tGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGt                                                                                      >  1:3888802/1‑100 (MQ=255)
        tGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGt                                                                                      >  2:3306940/1‑100 (MQ=255)
          gACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCt                                                                                    >  2:1136766/1‑100 (MQ=255)
          gACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCt                                                                                    >  1:2220439/1‑100 (MQ=255)
          gACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTAACTTCACAATTTGTTTACATCAATTTTAACAAATCATTAACAATCCGTc                                                                                     >  2:2392572/1‑99 (MQ=255)
           aCGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCtt                                                                                   <  1:3536615/100‑1 (MQ=255)
            cGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCttt                                                                                  >  1:120749/1‑100 (MQ=255)
             gATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCTGTCTTta                                                                                 <  1:2161001/100‑1 (MQ=255)
             gATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTta                                                                                 <  1:302530/100‑1 (MQ=255)
              aTTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGAAAGCACATTCACTTCACAATTTGTTTATATCAATTTTAACACATCATTAACAATCCGTCTTtat                                                                                <  2:1710416/100‑1 (MQ=255)
                tGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATAc                                                                              <  1:2873837/100‑1 (MQ=255)
                 gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACa                                                                             <  2:1212801/100‑1 (MQ=255)
                 gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACa                                                                             <  1:261899/100‑1 (MQ=255)
                 gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACa                                                                             >  1:67890/1‑100 (MQ=255)
                 gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACa                                                                             >  2:3357881/1‑100 (MQ=255)
                 gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACa                                                                             >  2:1340042/1‑100 (MQ=255)
                  agagTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAg                                                                            >  1:1207488/1‑100 (MQ=255)
                   tagTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGa                                                                           >  2:460196/2‑100 (MQ=255)
                   gagTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTATAACACATCATTAAAAATCAGTCTTTATACAGa                                                                           >  2:2383835/1‑100 (MQ=255)
                    agTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGAt                                                                          <  2:513543/100‑1 (MQ=255)
                      ttGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATc                                                                         <  2:470343/99‑1 (MQ=255)
                        gTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTa                                                                      >  1:461330/1‑100 (MQ=255)
                         tAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACGATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTaa                                                                     >  1:2796326/1‑100 (MQ=255)
                           aTGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAg                                                                   >  1:2854420/1‑100 (MQ=255)
                            tGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGa                                                                  <  1:2474358/100‑1 (MQ=255)
                            tGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACAAATCATTAACAATCCGTCTTTATACAGATCGTAAAg                                                                   <  2:821042/99‑1 (MQ=255)
                              aaGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGAtt                                                                >  1:3972518/1‑100 (MQ=255)
                                gTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGa                                                              >  1:3319352/1‑100 (MQ=255)
                                  cACTTATTTTCCCCGGAAGCACATTCACTTCACACTTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                            <  2:3501973/100‑1 (MQ=255)
                                  cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                            >  1:3613563/1‑100 (MQ=255)
                                  cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                            >  1:380559/1‑100 (MQ=255)
                                   aCTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGc                                                           >  1:3484483/1‑100 (MQ=255)
                                    cTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGcc                                                          <  2:2045280/100‑1 (MQ=255)
                                    cTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGcc                                                          <  2:3070979/100‑1 (MQ=255)
                                    cTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGcc                                                          <  2:3436976/100‑1 (MQ=255)
                                       aTTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCg                                                       <  2:1873109/100‑1 (MQ=255)
                                         tttCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGcc                                                     <  1:460200/100‑1 (MQ=255)
                                          ttCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCt                                                    >  1:1262532/1‑100 (MQ=255)
                                          ttCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCt                                                    <  2:2419872/100‑1 (MQ=255)
                                           tCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTg                                                   <  1:3665187/100‑1 (MQ=255)
                                            tcccGGAAGCACATGCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCTTAAAGATTGATGCCTCGcc                                                     >  2:608899/2‑97 (MQ=255)
                                              ccGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGt                                                >  1:3952859/1‑100 (MQ=255)
                                                ggAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTCAAGATTGATGCCTCGCATGCGTg                                               >  2:1428276/1‑99 (MQ=255)
                                                 gAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGc                                             >  2:1063544/1‑100 (MQ=255)
                                                 gAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGc                                             >  2:1063695/1‑100 (MQ=255)
                                                  aaGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGcc                                            <  1:3336308/100‑1 (MQ=255)
                                                    gCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCctc                                          >  1:977175/1‑100 (MQ=255)
                                                    gCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCctc                                          <  2:2316009/100‑1 (MQ=255)
                                                      acaTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTa                                        >  1:3872166/1‑100 (MQ=255)
                                                        atctacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                      <  1:3298778/96‑1 (MQ=255)
                                                        aTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                      >  1:1614368/1‑100 (MQ=255)
                                                        aTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                      <  1:2730661/100‑1 (MQ=255)
                                                          tcacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGc                                    <  1:3567253/100‑1 (MQ=255)
                                                          tcacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGc                                    <  2:2917113/100‑1 (MQ=255)
                                                          tcacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGc                                    <  1:3306960/100‑1 (MQ=255)
                                                          tcacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGc                                    <  1:3453527/100‑1 (MQ=255)
                                                          tcacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGc                                    <  1:2081760/100‑1 (MQ=255)
                                                           cacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGct                                   >  2:569533/1‑100 (MQ=255)
                                                            acttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATgagat                                  >  2:3298758/1‑96 (MQ=255)
                                                            acttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctc                                  >  2:1113021/1‑100 (MQ=255)
                                                                 acaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAtt                             >  2:2258314/1‑100 (MQ=255)
                                                                   aaTTTGTTTACATCAATGTTAACACATCATTAACAATCCGTCTTGATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCGCTGAt                              <  2:4112982/97‑1 (MQ=255)
                                                                       tGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTa                       <  2:3462174/100‑1 (MQ=255)
                                                                        gTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTaa                      <  1:2653634/100‑1 (MQ=255)
                                                                        gTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTaa                      >  1:320331/1‑100 (MQ=255)
                                                                        gTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTaa                      >  2:1302821/1‑100 (MQ=255)
                                                                         tttACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAat                     <  2:883544/100‑1 (MQ=255)
                                                                         tttACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAat                     <  2:2821609/100‑1 (MQ=255)
                                                                            aCATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATc                  <  2:1822629/100‑1 (MQ=255)
                                                                            aCATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATc                  <  2:2854404/100‑1 (MQ=255)
                                                                             cATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCt                 >  2:941971/1‑100 (MQ=255)
                                                                             cATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCt                 >  2:1714762/1‑100 (MQ=255)
                                                                              aTCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAACGATTGATGCCTCGCCTGCGTGGCCTCTATGGCCCTGCTTTACGTAATATCTg                >  1:3430824/1‑100 (MQ=255)
                                                                              aTCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTg                >  1:2640903/1‑100 (MQ=255)
                                                                              aTCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTg                >  1:2861820/1‑100 (MQ=255)
                                                                                cAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCg              >  1:3886456/1‑100 (MQ=255)
                                                                                cAATTTTAACACATCATTAACAATCCGTCTATACACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGCAATATCTGc               >  2:2721058/1‑99 (MQ=255)
                                                                                 aaTTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCgg             >  2:1080070/1‑100 (MQ=255)
                                                                                 aaTTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCgg             >  1:3243603/1‑100 (MQ=255)
                                                                                   ttttAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGc           >  2:1641906/1‑100 (MQ=255)
                                                                                    tttAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCg          <  2:302093/100‑1 (MQ=255)
                                                                                     ttAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGACGt         >  2:3797979/1‑100 (MQ=255)
                                                                                      tAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTg        <  1:245935/100‑1 (MQ=255)
                                                                                       aaCACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTgg       <  1:1340051/100‑1 (MQ=255)
                                                                                       aaCACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTgg       >  1:772285/1‑100 (MQ=255)
                                                                                        acacATCATTAACAATCCGTCTTTATACAGTTCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTgg       <  2:2041940/99‑1 (MQ=255)
                                                                                        acacATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGt      <  2:3432405/100‑1 (MQ=255)
                                                                                        acacATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGt      >  1:1702067/1‑100 (MQ=255)
                                                                                         caccTCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGc               >  2:2397535/1‑90 (MQ=255)
                                                                                          acaTCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGCTTTAAGTAATATCTGCGGGCGTGGTCa    >  2:1843968/1‑100 (MQ=255)
                                                                                           catcatTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCAc   >  2:2101363/1‑100 (MQ=255)
                                                                                                 |                                                                                              
GCAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACG  >  NC_000913/2278201‑2278392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: