Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,299,499 A→G 54.9% L27S (TTA→TCA)  napH ← ferredoxin‑type protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,299,4990AG54.9% 52.1 / 206.1 162L27S (TTA→TCA) napHferredoxin‑type protein
Reads supporting (aligned to +/- strand):  ref base A (38/35);  new base G (52/37);  total (90/72)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01

Only 100 of 163 total aligned reads displayed.

AATAACAGGCTACTGCTGTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCC  >  NC_000913/2299400‑2299597
                                                                                                   |                                                                                                  
  tAACAGGCTACTGCTGTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAac                                                                                                  <  2:3120760/100‑1 (MQ=255)
     cAGGCTACTGCTGTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACAcc                                                                                               <  1:717082/100‑1 (MQ=255)
     cAGGCTACTGCTGTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACAcc                                                                                               <  1:2259001/100‑1 (MQ=255)
     cAGGCTACTGCTGTAGTTGCCGTGCAAGATCCACACACAAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACAcc                                                                                               >  1:2278101/1‑100 (MQ=255)
       ggCTACTGCTGTAGTGGCTGTGCAAGATCCACACCCCGAACCATGGACCGCTCGAAAACAACCCCAGCACGAAGAACTAACAAAGGCGAAGAGACACCa                                                                                              >  2:3509498/1‑99 (MQ=255)
         cTACTGCTGTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGcc                                                                                           >  1:2475587/1‑100 (MQ=255)
         cTACTGCTGTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGcc                                                                                           <  2:4110070/100‑1 (MQ=255)
            ctgctgTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAAc                                                                                        >  2:1445345/1‑100 (MQ=255)
             tgctgTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACg                                                                                       <  2:536258/100‑1 (MQ=255)
              gctgTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACgg                                                                                      >  1:916532/1‑100 (MQ=255)
               ctgTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGt                                                                                     <  1:3468193/100‑1 (MQ=255)
                tgTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTg                                                                                    <  1:3016463/100‑1 (MQ=255)
                 gTAGTTGCCGTGCAAGATCCACACACCAACCCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACAAAAAAAGGCGACGTGCCACCAGCCAACg                                                                                       >  2:1246919/1‑96 (MQ=255)
                 gTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGAGACCAGCCAACGGTGa                                                                                   >  1:4073695/1‑100 (MQ=255)
                  tAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGAc                                                                                  <  1:2011244/100‑1 (MQ=255)
                  tAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGAc                                                                                  <  1:2328445/100‑1 (MQ=255)
                   aGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACt                                                                                 >  1:1191187/1‑100 (MQ=255)
                   aGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACt                                                                                 >  1:2306677/1‑100 (MQ=255)
                    gTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACt                                                                                 >  2:3374958/1‑99 (MQ=255)
                      tGCCGTGCAAGATCCCCACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAACGGCGACGTGACACCAGCCCACGGTGACTgcg                                                                              >  2:1052373/1‑100 (MQ=255)
                      tGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTgcg                                                                              >  1:602619/1‑100 (MQ=255)
                       gCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTgcgc                                                                             >  2:248723/1‑100 (MQ=255)
                        ccGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGcc                                                                            <  1:3385578/100‑1 (MQ=255)
                         cGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCCGCACGCAGAACTGACAAAGGCGACGTGAAACCAGCCAACGGTGGCTGCGcc                                                                            >  1:3748134/1‑99 (MQ=255)
                         cGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGcca                                                                           >  2:1130161/1‑100 (MQ=255)
                         cGTCCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGcca                                                                           <  2:1545569/100‑1 (MQ=255)
                          gTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGccac                                                                          >  1:1444535/1‑100 (MQ=255)
                          gTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGccac                                                                          >  1:1145411/1‑100 (MQ=255)
                           tGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGccacc                                                                         <  2:3666552/100‑1 (MQ=255)
                            gCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGccacca                                                                        >  1:3986569/1‑100 (MQ=255)
                             cAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAg                                                                       >  2:3976716/1‑100 (MQ=255)
                             cAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAg                                                                       <  2:3302164/100‑1 (MQ=255)
                             cAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAg                                                                       >  2:3400946/1‑100 (MQ=255)
                             cAAGATCCACACACCAAACCATGGACCGCTCAAAAACAGCCCCAGCACGAAGAACTGATAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAg                                                                       <  2:2255869/100‑1 (MQ=255)
                               aGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGcc                                                                     <  1:3976742/100‑1 (MQ=255)
                                gacccACACACCACCCCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCt                                                                    <  1:1827473/97‑1 (MQ=255)
                                   ccACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCtttt                                                                 <  1:3266654/100‑1 (MQ=255)
                                    cacacaCCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCttttt                                                                <  2:1906077/100‑1 (MQ=255)
                                    cacacaCCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCttttt                                                                <  1:1007796/100‑1 (MQ=255)
                                     acacacCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCtttttt                                                               >  1:2080043/1‑100 (MQ=255)
                                      cacacCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTtc                                                              <  2:1502681/100‑1 (MQ=255)
                                        cacCAAACCATGGCCCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTtctc                                                            >  1:2783203/1‑100 (MQ=255)
                                         acCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCCGCCTTTTTtctac                                                           <  1:2797866/100‑3 (MQ=255)
                                          ccAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGCCTGCGCCACCAGCCTTTTTTCTCCa                                                          >  1:1723926/1‑100 (MQ=255)
                                          ccAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCa                                                          >  1:130042/1‑100 (MQ=255)
                                           cAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAg                                                         >  1:2931928/1‑100 (MQ=255)
                                            aaaCCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAgc                                                        >  2:3993162/1‑100 (MQ=255)
                                               ccATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGcc                                                     >  2:494795/1‑100 (MQ=255)
                                               ccATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGcc                                                     >  1:2121190/1‑100 (MQ=255)
                                                 aTGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTc                                                   >  1:3278328/1‑100 (MQ=255)
                                                  tGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTcg                                                  <  1:3055787/100‑1 (MQ=255)
                                                  tGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTcg                                                  <  1:1647636/100‑1 (MQ=255)
                                                    gACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTTCATTGCCTcacg                                                >  2:1518025/1‑100 (MQ=255)
                                                    gACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTcgcg                                                >  2:92331/1‑100 (MQ=255)
                                                     aCCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCgcgc                                               <  2:1099070/100‑1 (MQ=255)
                                                       cGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGccc                                             <  2:747964/100‑1 (MQ=255)
                                                        gCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCACCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCg                                            <  1:1424755/100‑1 (MQ=255)
                                                         cTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCgg                                           >  2:4026662/1‑100 (MQ=255)
                                                           cAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCg                                         >  2:2059726/1‑100 (MQ=255)
                                                           cAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCg                                         <  1:4026688/100‑1 (MQ=255)
                                                           cAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCg                                         >  1:3198300/1‑100 (MQ=255)
                                                           cAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCg                                         >  2:3984928/1‑100 (MQ=255)
                                                            aaaaaCATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGt                                        >  1:1783437/1‑100 (MQ=255)
                                                            aaaaaCATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGt                                        >  2:2180871/1‑100 (MQ=255)
                                                              aaaCATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCa                                      >  2:1848018/1‑100 (MQ=255)
                                                                aCATCCCCAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACg                                    <  1:1036477/100‑1 (MQ=255)
                                                                    ccccAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTa                                >  1:74962/1‑100 (MQ=255)
                                                                     cccAGCACGAAGAGCTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTAc                               <  2:3349905/100‑1 (MQ=255)
                                                                     cccAGCACGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTAc                               >  2:808695/1‑100 (MQ=255)
                                                                     cccAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTAc                               >  1:3504273/1‑100 (MQ=255)
                                                                     cccAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTAc                               >  1:4123021/1‑100 (MQ=255)
                                                                            cGAAGAACTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTTTTTTTTTTTTTGCGCCCGGCGTCACGTTTACGAttt                          >  2:2703509/1‑98 (MQ=255)
                                                                            cGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGc                        >  2:2190740/1‑100 (MQ=255)
                                                                            cGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGc                        <  1:2051568/100‑1 (MQ=255)
                                                                            cGAAGAACTGACAAAGGCGACGTAACAACAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTAGCGCCCGGTGTCACGATTACGAttt                          >  2:1181342/1‑98 (MQ=255)
                                                                             gaagaaCTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGcc                       <  1:1569816/100‑1 (MQ=255)
                                                                             gaagaaCTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGcc                       <  1:308431/100‑1 (MQ=255)
                                                                               agaaCTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCAt                     <  2:3452073/100‑1 (MQ=255)
                                                                                  aCTGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGt                  <  1:92332/100‑1 (MQ=255)
                                                                                  aCTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGt                  <  2:1170094/100‑1 (MQ=255)
                                                                                    tGACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTc                <  2:1622470/100‑1 (MQ=255)
                                                                                     gACAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTcc               <  1:233746/100‑1 (MQ=255)
                                                                                     gACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTcc               <  1:3325764/100‑1 (MQ=255)
                                                                                     gACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTcc               <  2:3659903/100‑1 (MQ=255)
                                                                                      aCAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTccc              <  2:2747802/100‑1 (MQ=255)
                                                                                       cAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTcccc             >  1:792705/1‑100 (MQ=255)
                                                                                       cAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTcccc             <  1:1108057/100‑1 (MQ=255)
                                                                                       cAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTcccc             >  1:3146818/1‑100 (MQ=255)
                                                                                       cAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTcccc             >  1:2960586/1‑100 (MQ=255)
                                                                                       cAAAGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTcccc             <  2:3734173/100‑1 (MQ=255)
                                                                                       cAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTcccc             <  2:3089628/100‑1 (MQ=255)
                                                                                       cAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTcccc             <  1:2771576/100‑1 (MQ=255)
                                                                                        aaaGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCt            <  1:1996313/100‑1 (MQ=255)
                                                                                          aGGCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTcc          >  1:1890527/1‑100 (MQ=255)
                                                                                           ggCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTcca         >  1:3710170/1‑100 (MQ=255)
                                                                                            gCGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTccag        <  1:1222812/100‑1 (MQ=255)
                                                                                            gCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTccag        <  2:666301/100‑1 (MQ=255)
                                                                                             cGACGTGACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTccagc       <  2:2924742/100‑1 (MQ=255)
                                                                                             cGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTccagc       <  2:2307329/100‑1 (MQ=255)
                                                                                                 gTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCcagccagc   >  1:2158125/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
AATAACAGGCTACTGCTGTAGTTGCCGTGCAAGATCCACACACCAAACCATGGACCGCTCAAAAACATCCCCAGCACGAAGAACTGACAAAGGCGACGTAACACCAGCCAACGGTGACTGCGCCACCAGCCTTTTTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCC  >  NC_000913/2299400‑2299597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑