Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,307,386 A→T 77.8% D410E (GAT→GAA yojI ← microcin J25 efflux ABC transporter permease/ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,307,3860AT77.8% 286.1 / 76.0 139D410E (GAT→GAAyojImicrocin J25 efflux ABC transporter permease/ATPase
Reads supporting (aligned to +/- strand):  ref base A (19/12);  new base T (60/48);  total (79/60)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 141 total aligned reads displayed.

CGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCG  >  NC_000913/2307289‑2307485
                                                                                                 |                                                                                                   
  ttGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCa                                                                                                  <  2:3576565/99‑1 (MQ=255)
    gCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAAc                                                                                               >  1:2474110/1‑100 (MQ=255)
    gCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAAc                                                                                               >  2:2707042/1‑100 (MQ=255)
    gCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAAc                                                                                               >  1:4104039/1‑100 (MQ=255)
    gCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAAc                                                                                               >  2:2726917/1‑100 (MQ=255)
     cTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACa                                                                                              >  1:2219066/1‑100 (MQ=255)
       tAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGTCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGc                                                                                            >  1:2920479/1‑100 (MQ=255)
        aaCTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGcc                                                                                           <  1:617438/100‑1 (MQ=255)
         aCTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCa                                                                                          >  1:3649942/1‑100 (MQ=255)
          cTCACGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAg                                                                                         <  2:2593288/100‑1 (MQ=255)
          cTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAg                                                                                         >  1:150191/1‑100 (MQ=255)
          cTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAg                                                                                         <  1:2420227/100‑1 (MQ=255)
           tCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGa                                                                                        <  1:900193/100‑1 (MQ=255)
            cAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGAc                                                                                       >  2:2872909/1‑100 (MQ=255)
              aGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACAt                                                                                     >  1:3792814/1‑100 (MQ=255)
                cTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCg                                                                                   >  1:1933215/1‑100 (MQ=255)
                cTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCg                                                                                   >  2:2436554/1‑100 (MQ=255)
                    tGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTaa                                                                               >  1:2419717/1‑100 (MQ=255)
                     gAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTaaa                                                                              >  1:906808/1‑100 (MQ=255)
                      aGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAac                                                                             >  1:3536198/1‑100 (MQ=255)
                      aGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAac                                                                             <  2:315615/100‑1 (MQ=255)
                      aGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAac                                                                             <  1:1090075/100‑1 (MQ=255)
                       ggcATGTTCAGCTGCGCCAGCCACTTCTCGCCCAGTTGCGGGTTAGCGGGTTCACCCTTCGGCACAAGCAGTTGTTCAAACAGCCAGACATCGGTaaa                                                                              <  2:1845840/96‑1 (MQ=255)
                       gCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAaca                                                                            >  2:1720300/1‑100 (MQ=255)
                       gCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAaca                                                                            >  2:1668081/1‑100 (MQ=255)
                            tttCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGcc                                                                       <  2:1184516/100‑1 (MQ=255)
                            ttgcAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGca                                                                       <  2:2295459/97‑2 (MQ=255)
                               cAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTACCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGCAAACACTGCCg                                                                      >  2:3586643/1‑98 (MQ=255)
                                aGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGaaa                                                                   <  1:94499/100‑1 (MQ=255)
                                aGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGaaa                                                                   <  2:3182390/100‑1 (MQ=255)
                                aGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGaaa                                                                   <  1:183612/100‑1 (MQ=255)
                                aGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGaaa                                                                   >  1:2621150/1‑100 (MQ=255)
                                aGCTGCCCCACCCACTTCTCAACCAGTTGCAGGACAGCGGGCCTCCCCTCCCGCCCCCGCCGTTGTTCAAACAGGCAGACATCGGTAAaccacgcc                                                                       >  2:325823/1‑90 (MQ=255)
                                 gCTGCGCGAGCTACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTCCAAACAGCCAGACATCGGTAAACACTGCCGaaaa                                                                  >  2:2164445/1‑100 (MQ=255)
                                  cTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAAc                                                                 <  1:39914/100‑1 (MQ=255)
                                  cTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAAc                                                                 <  1:1791011/100‑1 (MQ=255)
                                    gcgcCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAg                                                               <  1:1190201/100‑1 (MQ=255)
                                     cgcCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGt                                                              <  2:2092548/100‑1 (MQ=255)
                                      gccagccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACCCTGCCGAAAACAGtt                                                             >  2:2272768/1‑100 (MQ=255)
                                       ccagccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCCGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGttt                                                            >  2:1398960/1‑100 (MQ=255)
                                         acccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGc                                                          <  2:2509632/98‑1 (MQ=255)
                                          gccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCg                                                         >  1:3395723/1‑100 (MQ=255)
                                          gccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCg                                                         <  2:632238/100‑1 (MQ=255)
                                              cTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATa                                                      <  2:1942987/99‑1 (MQ=255)
                                               ttCTCAATCAGTTGCGGGTTAGCGGGTTTACCCTCCAGCCCCAGCATGTGTACAAACAGCCAAACATCGTCCAACACCGCCAAAAACAGATTTCGaaa                                                      >  2:762238/1‑96 (MQ=255)
                                               ttCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAAt                                                    <  1:3039474/100‑1 (MQ=255)
                                               ttCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAAt                                                    >  2:897828/1‑100 (MQ=255)
                                               ttCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAAt                                                    <  2:115438/100‑1 (MQ=255)
                                                tctcAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATc                                                   <  2:1440894/100‑1 (MQ=255)
                                                tctcAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATc                                                   >  2:3405181/1‑100 (MQ=255)
                                                 ctcAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCt                                                  >  2:2857990/1‑100 (MQ=255)
                                                  tcAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCtt                                                 >  1:472447/1‑100 (MQ=255)
                                                  tcAACCAGTTACGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCtt                                                 >  2:3277523/1‑100 (MQ=255)
                                                   cAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTc                                                >  1:2971534/1‑100 (MQ=255)
                                                   cAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAAAAGTTTGCGATAATCTTc                                                >  2:3930428/1‑100 (MQ=255)
                                                   cAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCACCAGTTGTTCAAACAGCCGGACCTCGGTAAACACTGCCGAACACAGTTTGCGATAATCTTc                                                >  2:1997106/1‑100 (MQ=255)
                                                    aaCCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTcc                                               >  1:2692069/1‑100 (MQ=255)
                                                    aaCCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTcc                                               >  2:3815609/1‑100 (MQ=255)
                                                      ccAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCgg                                             <  1:599716/100‑1 (MQ=255)
                                                      ccAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCgg                                             <  1:2389991/100‑1 (MQ=255)
                                                      ccAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCgg                                             >  2:1946300/1‑100 (MQ=255)
                                                      ccAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCgg                                             >  1:305415/1‑100 (MQ=255)
                                                       cAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgt                                            <  1:753134/100‑1 (MQ=255)
                                                        aGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGGCATCGGTAAACACTGCCGAAAACAGTGTGCGATAATCTTCCGgtt                                           >  1:2251276/1‑100 (MQ=255)
                                                         gTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgttg                                          >  1:789232/1‑100 (MQ=255)
                                                          ttGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgttgt                                         <  2:1631054/100‑1 (MQ=255)
                                                           tGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgttgtt                                        >  2:1203641/1‑100 (MQ=255)
                                                           tGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgttgtt                                        <  1:1660421/100‑1 (MQ=255)
                                                           tGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAAAAGTTTGCGATAATCTTCCGgttgtt                                        >  2:2489871/1‑100 (MQ=255)
                                                            gCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTc                                       >  1:1041181/1‑100 (MQ=255)
                                                             cGGGTTAGCGGGTTTACTCTCCAGACCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcg                                      >  2:1836299/1‑100 (MQ=255)
                                                               ggTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgcc                                    >  2:3751924/1‑100 (MQ=255)
                                                                gTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgccg                                   <  1:3930454/100‑1 (MQ=255)
                                                                  tAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACCTCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgccgc                                  >  2:852787/1‑99 (MQ=255)
                                                                   aGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTg                                >  1:1492801/1‑100 (MQ=255)
                                                                   aGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTg                                >  1:1277182/1‑100 (MQ=255)
                                                                    gCGGGTTTGACGTACGGCACCAGCTGTTGTTCAAACAGCCCGACGTCGGTAAAAACGGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgccgc                                  <  2:2758362/97‑1 (MQ=255)
                                                                    gCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTg                                >  2:483285/1‑99 (MQ=255)
                                                                    gCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTg                                >  1:3880265/1‑99 (MQ=255)
                                                                    gCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGa                               >  2:968506/1‑100 (MQ=255)
                                                                    gCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGa                               >  2:967723/1‑100 (MQ=255)
                                                                     cGGGTTTGCCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGAc                              <  2:795891/100‑1 (MQ=255)
                                                                     cGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGAc                              <  1:1562515/100‑1 (MQ=255)
                                                                       ggTTTACCCTCCGGCCCCAGCCGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATACTCTTCCGGTTGTTCGCCGCTGa                               >  2:3416040/1‑97 (MQ=255)
                                                                           tACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGttt                        <  1:4003595/100‑1 (MQ=255)
                                                                            aCCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTg                       >  1:407543/1‑100 (MQ=255)
                                                                             cccTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTCAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGAcccatctgc                      >  2:3389901/1‑92 (MQ=255)
                                                                             cccTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGc                      >  1:3972268/1‑100 (MQ=255)
                                                                             cccTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGc                      >  2:753261/1‑100 (MQ=255)
                                                                              ccTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGcc                     >  1:52154/1‑100 (MQ=255)
                                                                               cTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCa                    <  1:2615933/100‑1 (MQ=255)
                                                                                 ccGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATc                  >  1:728319/1‑100 (MQ=255)
                                                                                   ggCCCCAGCAGTTGTTGAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCa                <  2:2420726/100‑1 (MQ=255)
                                                                                     ccccAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGc              <  1:1048854/100‑1 (MQ=255)
                                                                                     ccccAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGc              >  1:2957858/1‑100 (MQ=255)
                                                                                      cccAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTACCATCCAGCa             <  1:1532095/100‑1 (MQ=255)
                                                                                        cagcagTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCaan           <  1:3483506/100‑2 (MQ=255)
                                                                                         agcagTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGa          <  2:2222801/100‑1 (MQ=255)
                                                                                               tGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTcgc    <  1:3545291/100‑1 (MQ=255)
                                                                                                 aTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTcgccg  <  2:1162164/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
CGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCG  >  NC_000913/2307289‑2307485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: