Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,461,483 G→T 55.4% A60S (GCA→TCA)  fadL → long‑chain fatty acid outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,461,4830GT55.4% 30.6 / 199.9 141A60S (GCA→TCA) fadLlong‑chain fatty acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (32/31);  new base T (34/44);  total (66/75)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.31e-01

Only 100 of 143 total aligned reads displayed.

GGCTTTCAGTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTAGCC  >  NC_000913/2461384‑2461582
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ggCTTTCAGTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCg                                                                                                     >  2:712757/1‑100 (MQ=255)
   tttCAGTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCAt                                                                                                  <  1:3264129/100‑1 (MQ=255)
    ttCAGTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCAtt                                                                                                 <  1:473595/100‑1 (MQ=255)
      cAGTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGAGGCAGGTAACGTCAGCCGTAACCCCGCATTGa                                                                                               >  2:879146/1‑100 (MQ=255)
       aGTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGAt                                                                                              <  2:2449961/100‑1 (MQ=255)
        gTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGAtt                                                                                             >  2:2607502/1‑100 (MQ=255)
        gTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGAtt                                                                                             <  2:1596947/100‑1 (MQ=255)
        gTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGAtt                                                                                             <  1:1433957/100‑1 (MQ=255)
        gTTAAACGAATTTTCTTCCTCTAGCCTGGGCCGGGCTTATTCAGGTGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGAtt                                                                                             >  2:473061/1‑100 (MQ=255)
         ttAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCCGCCGCAACCCCTCATCGAtt                                                                                             <  2:829705/99‑1 (MQ=255)
          tAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTAc                                                                                           <  1:2464344/100‑1 (MQ=255)
          tAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTAc                                                                                           >  2:1871994/1‑100 (MQ=255)
           aaaCGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTCTTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACt                                                                                          >  1:2964890/1‑100 (MQ=255)
            aaCGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTa                                                                                         <  2:3576104/100‑1 (MQ=255)
            aaCGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTa                                                                                         >  2:2800609/1‑100 (MQ=255)
             aCGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTAt                                                                                        >  2:4101787/1‑100 (MQ=255)
             aCGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTAt                                                                                        >  2:230109/1‑100 (MQ=255)
               gAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGt                                                                                      >  1:2104131/1‑100 (MQ=255)
                aaTTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGtt                                                                                     <  2:72286/100‑1 (MQ=255)
                 aTTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGttt                                                                                    <  1:2607518/100‑1 (MQ=255)
                     tcttcCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGAcc                                                                                <  2:2429310/100‑1 (MQ=255)
                      cttcCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCg                                                                               >  1:3951007/1‑100 (MQ=255)
                      cttcCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCg                                                                               >  1:2956385/1‑100 (MQ=255)
                      cttcCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCg                                                                               <  1:119250/100‑1 (MQ=255)
                        tcCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGcc                                                                             <  1:2340877/100‑1 (MQ=255)
                         ccTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGccc                                                                            <  1:1814700/100‑1 (MQ=255)
                         ccTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGccc                                                                            >  2:1903887/1‑100 (MQ=255)
                          ctctGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCg                                                                           <  1:1143016/100‑1 (MQ=255)
                          ctctGGCCTGCGCCGGGCTTACTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTCACCGccc                                                                            >  2:1220251/1‑99 (MQ=255)
                             aggCCTGAGCAGGGCTTATTCAGGGTAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGAc                                                                         >  2:623124/2‑99 (MQ=255)
                              ggCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACAt                                                                       >  1:1059532/1‑100 (MQ=255)
                               gCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACAtt                                                                      >  1:1023159/1‑100 (MQ=255)
                                ccTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACAttt                                                                     <  1:4043541/100‑1 (MQ=255)
                                   gggCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCt                                                                  >  1:307014/1‑100 (MQ=255)
                                     gCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGc                                                                <  1:3816862/100‑1 (MQ=255)
                                     gCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGc                                                                >  1:1327589/1‑100 (MQ=255)
                                      ccGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCg                                                               >  1:1474955/1‑100 (MQ=255)
                                      ccGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCg                                                               <  1:198282/100‑1 (MQ=255)
                                       cgagCTTATTCAGGGGAAGACGCAAGTGCCGATGATGCAGATAACGTCAGCCGTAACTCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTg                                                                 <  2:364468/94‑1 (MQ=255)
                                       cGGGCTTATTCAGGGGAAGTCGCAATTGCAGATGATGCAGGTAACGGCAGCCGTAACCCCTCATGGATTACTATGTTGGACCGCCCGACATTTTCTGCgg                                                              <  2:1998716/100‑1 (MQ=255)
                                        gttggtATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCggg                                                             <  1:3143732/95‑1 (MQ=255)
                                             tATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCgg                                                        >  2:3083810/1‑100 (MQ=255)
                                              aTTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGt                                                       >  1:2491400/1‑100 (MQ=255)
                                               ttCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGtt                                                      >  2:3548090/1‑100 (MQ=255)
                                                 cAGGGGAAGGCGCAATTGCCGATGATGCAGGTAGCGTCAGCCGTAACCCCGCATTGATTACTATGTTTGAGCGCCCGACATTTTCTGCGGGTGCGGTTta                                                    >  2:2482166/1‑100 (MQ=255)
                                                 cAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTta                                                    <  1:2648371/100‑1 (MQ=255)
                                                  aGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTtat                                                   <  1:2956515/100‑1 (MQ=255)
                                                    gggAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTatat                                                 <  1:4028147/100‑1 (MQ=255)
                                                     gggaGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATAtt                                                <  1:1544158/97‑1 (MQ=255)
                                                        aGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGAc                                             <  1:405295/100‑1 (MQ=255)
                                                        aGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGAc                                             <  1:405323/100‑1 (MQ=255)
                                                         ggCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGAcc                                            >  2:1306583/1‑100 (MQ=255)
                                                         ggCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTCTGTTTGACCGCCCGACGTTTTCTGCGGGTGCGGTTTATATTGAcc                                            >  1:995272/1‑100 (MQ=255)
                                                         ggCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGAcc                                            <  1:1665244/100‑1 (MQ=255)
                                                           cgcAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCg                                          <  1:3196955/100‑1 (MQ=255)
                                                             cAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGCCCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGa                                        <  1:3034201/100‑1 (MQ=255)
                                                              aaTTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGAt                                       <  1:3030366/100‑1 (MQ=255)
                                                              aaTTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGAt                                       <  1:1585819/100‑1 (MQ=255)
                                                              aaTTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGAt                                       <  2:3908135/100‑1 (MQ=255)
                                                               aTTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATg                                      <  1:858647/100‑1 (MQ=255)
                                                               aTTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATg                                      <  1:1015950/100‑1 (MQ=255)
                                                                 tGCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTa                                    >  2:2152037/1‑100 (MQ=255)
                                                                  gCCGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTaa                                   >  2:1137405/1‑100 (MQ=255)
                                                                   ccGATGATGCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTaaa                                  <  1:1231293/100‑1 (MQ=255)
                                                                   ccGATGATGCAGATAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTaaa                                  >  1:2713347/1‑100 (MQ=255)
                                                                    cGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAat                                 <  2:1488368/100‑1 (MQ=255)
                                                                     gatgatGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAata                                <  2:1606333/100‑1 (MQ=255)
                                                                     gatgatGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAata                                >  2:3647887/1‑100 (MQ=255)
                                                                      atgatgCAGGTAACGTCAGCCGTAGCTCCGCATTGGGTACTGTGTTGGCCCGCCCGACATTTGCTGCGGGTGCGGTTTATATTGACCCGGATGTAAatct                               <  2:1854385/100‑3 (MQ=255)
                                                                         atgCAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAg                            >  2:3660718/1‑100 (MQ=255)
                                                                         atgCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTACGGGTGCGGTTTATATTGACCCGGATGTAAATATCAg                            >  1:2409094/1‑100 (MQ=255)
                                                                           gCAGGTAACGTCAGCCGTAACCACTCATTGATTACTATGTTTGACCGTCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCg                          <  2:2795731/100‑1 (MQ=255)
                                                                            cAGGTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCgg                         <  1:331178/100‑1 (MQ=255)
                                                                            cAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCgg                         <  1:869406/100‑1 (MQ=255)
                                                                               gTAACGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAAc                      >  2:1675713/1‑100 (MQ=255)
                                                                               gTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAAc                      <  1:1423271/100‑1 (MQ=255)
                                                                                 aaCGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGt                    <  1:2457840/100‑1 (MQ=255)
                                                                                  aCGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCCGTTTATATTGACCCGGATGTAAATTTCAGCGGAACGtc                   <  2:2550542/100‑1 (MQ=255)
                                                                                   cGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGtct                  <  2:4018306/100‑1 (MQ=255)
                                                                                   cGTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGtct                  <  2:2013464/100‑1 (MQ=255)
                                                                                    gTCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGtctc                 >  1:1701348/1‑100 (MQ=255)
                                                                                    gTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGtctc                 >  2:3364765/1‑100 (MQ=255)
                                                                                    gTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGtctc                 >  2:3364898/1‑100 (MQ=255)
                                                                                    gTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGAGTGCGGTTTATATTGACCCGGATGTAAACATCAGCGGAACGtctc                 >  2:3833716/1‑100 (MQ=255)
                                                                                     tCAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTcc                <  1:805329/100‑1 (MQ=255)
                                                                                     tCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTcc                <  1:1894575/100‑1 (MQ=255)
                                                                                      cAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCa               <  1:3524252/100‑1 (MQ=255)
                                                                                      cAGCCGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCa               >  2:651159/1‑100 (MQ=255)
                                                                                      cAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTTCGGGTGCGGTTTATATTGACCCGGATGTAAATATCCGCGGAACGTCTcc                >  2:925131/1‑99 (MQ=255)
                                                                                        gCCGTAACCCCGCCTTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGGTGATATTGACCGGGCTGTAAATATCAGCGGAACGTCTCCATc             >  1:483797/1‑100 (MQ=255)
                                                                                        gCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATc             >  2:1389080/1‑100 (MQ=255)
                                                                                          cGTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTg           >  2:1725533/1‑100 (MQ=255)
                                                                                          cGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTg           >  1:857287/1‑100 (MQ=255)
                                                                                           gTAACCCCTCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTgg          <  2:140158/100‑1 (MQ=255)
                                                                                            tAACCCCTCATTGATTACTATGTGTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGt         <  1:782828/100‑1 (MQ=255)
                                                                                             agccccTCATTGTTTACTACGTTTGACCGCCCGACATTTTCTGCGGCGGTGGTTTATATTGGCCCGGATGTAAATATCAGCCGAACGTCTCCATCTGGTc        <  2:323158/98‑1 (MQ=255)
                                                                                                cccGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTa     >  2:3079784/1‑100 (MQ=255)
                                                                                                cccGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTa     >  1:2202744/1‑100 (MQ=255)
                                                                                                cccGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTa     >  1:2901252/1‑100 (MQ=255)
                                                                                                   gCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGCCCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTAGcc  <  2:9103/100‑1 (MQ=255)
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GGCTTTCAGTTAAACGAATTTTCTTCCTCTGGCCTGGGCCGGGCTTATTCAGGGGAAGGCGCAATTGCCGATGATGCAGGTAACGTCAGCCGTAACCCCGCATTGATTACTATGTTTGACCGCCCGACATTTTCTGCGGGTGCGGTTTATATTGACCCGGATGTAAATATCAGCGGAACGTCTCCATCTGGTCGTAGCC  >  NC_000913/2461384‑2461582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: