Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,521,967 C→A 100% A171S (GCG→TCG)  xapR ← transcriptional activator of xapAB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,521,9670CA99.3% 458.2 / ‑4.0 136A171S (GCG→TCG) xapRtranscriptional activator of xapAB
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (68/67);  total (69/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

Only 100 of 141 total aligned reads displayed.

GGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCAT  >  NC_000913/2521870‑2522063
                                                                                                 |                                                                                                
ggcggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAacgacg                                                                                                <  2:1110444/100‑1 (MQ=255)
 gcggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAAcgacga                                                                                               >  2:2729575/1‑100 (MQ=255)
 gcggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGAATAGCCAACCGAAacgacgc                                                                                               >  1:2829191/1‑99 (MQ=255)
   ggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGAtt                                                                                             <  1:787954/100‑1 (MQ=255)
     cATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCa                                                                                           <  1:3299685/100‑1 (MQ=255)
     cATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCa                                                                                           >  1:3085143/1‑100 (MQ=255)
       ttGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATg                                                                                         <  2:720478/100‑1 (MQ=255)
        tGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGc                                                                                        <  2:1206529/100‑1 (MQ=255)
        tGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGc                                                                                        <  1:1668362/100‑1 (MQ=255)
            aCAAAATCCTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAAcc                                                                                    <  1:3734216/100‑1 (MQ=255)
             cAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCg                                                                                   <  2:1710552/100‑1 (MQ=255)
             cAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCg                                                                                   >  1:3005913/1‑100 (MQ=255)
               aaaTACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTa                                                                                 >  1:1438562/1‑100 (MQ=255)
               aaaTACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTa                                                                                 <  1:3990607/100‑1 (MQ=255)
                    cTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTg                                                                            >  2:3768099/1‑100 (MQ=255)
                     tCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTgg                                                                           <  2:3442623/100‑1 (MQ=255)
                     tCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTgg                                                                           <  1:1595455/100‑1 (MQ=255)
                      cGTCACGTAGCGCTTCCAGCGGGACGGTGGAACATGATGGGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGt                                                                          <  2:2904180/100‑1 (MQ=255)
                      cGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGt                                                                          <  2:2390495/100‑1 (MQ=255)
                       gTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTa                                                                         <  2:3085124/100‑1 (MQ=255)
                       gTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTa                                                                         >  2:442093/1‑100 (MQ=255)
                        tCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTaa                                                                        >  2:1439645/1‑100 (MQ=255)
                         cACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTaaa                                                                       >  1:824669/1‑100 (MQ=255)
                         cACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTaaa                                                                       >  1:209456/1‑100 (MQ=255)
                         cACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTaaa                                                                       >  1:90319/1‑100 (MQ=255)
                            gTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAcc                                                                    <  2:372928/100‑1 (MQ=255)
                            gTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAcc                                                                    <  1:2275002/100‑1 (MQ=255)
                              aGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAg                                                                  >  1:158502/1‑100 (MQ=255)
                              aGCGCTTCCAGCGGGACGGAGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGAAGATTCATGCAACCGTAAGCTGGTAAAACAAg                                                                  >  2:2784360/1‑100 (MQ=255)
                               gcgcTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGt                                                                 <  2:2345256/100‑1 (MQ=255)
                                cgcTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTc                                                                >  1:423422/1‑100 (MQ=255)
                                cgcTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTc                                                                <  2:3221909/100‑1 (MQ=255)
                                  cTTCCAGCAGAACGGTGGAAAATGACAGGAGATGATCCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCATTCg                                                               <  2:61462/99‑1 (MQ=255)
                                    tCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtg                                                            <  2:3951780/100‑1 (MQ=255)
                                     ccAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtgg                                                           >  1:2220912/1‑100 (MQ=255)
                                      cAGCGGGACGGTGGACAGTGATGAGAGATGATGCTGTTAAGGCATCGCCACCAGCAACGATGCTTCATGCAACGGTAAGCTGGTAAAACGAGTCggtggt                                                          >  2:2443857/1‑100 (MQ=255)
                                      cAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCGACCAGAAACGACGAGTGATGCAACCGTAAGCTGGTAAAACCAGTCggtggt                                                          <  2:3888337/100‑1 (MQ=255)
                                      cAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCggtggt                                                          >  2:468561/1‑100 (MQ=255)
                                       aGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGtt                                                         <  1:1740966/100‑1 (MQ=255)
                                          gggACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTg                                                      <  2:3682831/100‑1 (MQ=255)
                                             aCGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCg                                                   <  2:1075895/100‑1 (MQ=255)
                                              cGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGc                                                  <  2:814820/100‑1 (MQ=255)
                                               ggTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGcc                                                 >  1:179376/1‑100 (MQ=255)
                                               ggTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGcc                                                 >  2:90512/1‑100 (MQ=255)
                                                gTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGcc                                                 >  2:418500/1‑99 (MQ=255)
                                                 tGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCAt                                               >  1:1072340/1‑100 (MQ=255)
                                                  ggAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCAtt                                              >  1:811730/1‑100 (MQ=255)
                                                  ggAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCAtt                                              >  2:2051200/1‑100 (MQ=255)
                                                  ggAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCAtt                                              >  1:3490753/1‑100 (MQ=255)
                                                      aaTGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGcc                                          <  1:4030199/100‑1 (MQ=255)
                                                        tgatgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAg                                        <  2:1693394/100‑1 (MQ=255)
                                                        tgatgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAg                                        >  1:1310451/1‑100 (MQ=255)
                                                        tgatgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAg                                        >  1:970778/1‑100 (MQ=255)
                                                            gagagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTCCTGTCGCCATTCGCCAGATcc                                    >  1:3869416/1‑100 (MQ=255)
                                                             agagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATccc                                   >  1:1831507/1‑100 (MQ=255)
                                                             agagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATccc                                   >  1:2892388/1‑100 (MQ=255)
                                                              gagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCg                                  <  2:3394568/100‑1 (MQ=255)
                                                              gagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCg                                  >  1:869032/1‑100 (MQ=255)
                                                               agaTGATGCTCTTCAGGCATCGCCACCAGATTTGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGTTTCTGTCGCCATTCGCCACATCCCg                                  <  2:1020064/99‑1 (MQ=255)
                                                                gatgatGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGc                                <  1:2559355/100‑1 (MQ=255)
                                                                gatgatGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGc                                >  1:781020/1‑100 (MQ=255)
                                                                 atgatgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCa                               <  1:1057826/100‑1 (MQ=255)
                                                                  tgatgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCAt                              <  1:3421591/100‑1 (MQ=255)
                                                                  tgatgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCAt                              <  1:2706798/100‑1 (MQ=255)
                                                                  tgacgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCAt                              >  1:2340768/1‑100 (MQ=255)
                                                                   gatgCTCTTCAGGCATCGCCACCCGACACGACGATTCATGCAACCCTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATc                             <  2:3443708/100‑1 (MQ=255)
                                                                      gCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCaan                          <  2:3560011/100‑2 (MQ=255)
                                                                      gCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAg                          <  1:3134166/100‑1 (MQ=255)
                                                                       ctctTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGt                         >  1:3244635/1‑100 (MQ=255)
                                                                       ctctTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGt                         >  1:1497440/1‑100 (MQ=255)
                                                                       ctattCAGGCATCGCTACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTGGGTGGTTATGTCGCCATTCTTCAGATCCCGGCATCAAg                          <  2:2610418/96‑1 (MQ=255)
                                                                        tctTCAGGCATCGCCGCCAGAAACGACGATTTATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGt                         <  2:3072498/99‑1 (MQ=255)
                                                                        tctTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGtt                        >  2:2819417/1‑100 (MQ=255)
                                                                         ctTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTc                       >  2:1656144/1‑100 (MQ=255)
                                                                         ctTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTc                       >  2:3643650/1‑100 (MQ=255)
                                                                           tCAGTCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCTGCATCAAGTTcgc                     <  2:3148055/100‑1 (MQ=255)
                                                                           tCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgc                     <  1:815441/100‑1 (MQ=255)
                                                                           tCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgc                     >  2:3950395/1‑100 (MQ=255)
                                                                            cAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgcg                    <  1:2527911/100‑1 (MQ=255)
                                                                             aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                   <  1:3135417/100‑1 (MQ=255)
                                                                             aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                   <  2:1043325/100‑1 (MQ=255)
                                                                             aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                   >  2:1920498/1‑100 (MQ=255)
                                                                             aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                   >  1:1651779/1‑100 (MQ=255)
                                                                              ggCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcggc                  >  2:290031/1‑100 (MQ=255)
                                                                               gCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcggcg                 >  1:524578/1‑100 (MQ=255)
                                                                                cATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGt                <  2:1060820/100‑1 (MQ=255)
                                                                                  tCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTc              <  1:1550207/100‑1 (MQ=255)
                                                                                    gCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCa            <  2:913789/100‑1 (MQ=255)
                                                                                     ccaccaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAg           >  1:4134110/1‑100 (MQ=255)
                                                                                     ccaccaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAg           <  1:1932927/100‑1 (MQ=255)
                                                                                      caccaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGc          <  2:1651767/100‑1 (MQ=255)
                                                                                        ccaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCaa        <  2:4134083/100‑1 (MQ=255)
                                                                                        ccaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCaa        >  1:554503/1‑100 (MQ=255)
                                                                                         caGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAg       >  2:1028472/1‑100 (MQ=255)
                                                                                         caGAAACGACGACTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAg       >  1:2715572/1‑100 (MQ=255)
                                                                                          aGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAgg      <  1:3437487/100‑1 (MQ=255)
                                                                                           gAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGc     <  1:398554/100‑1 (MQ=255)
                                                                                            aaaCGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGcc    >  1:2961636/1‑100 (MQ=255)
                                                                                             aaCGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCa   >  2:1413730/1‑100 (MQ=255)
                                                                                             aaCGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCa   <  1:2864104/100‑1 (MQ=255)
                                                                                                 |                                                                                                
GGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCAT  >  NC_000913/2521870‑2522063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: