Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 53,012 A→G 24.2% G135G (GGT→GGC pdxA ← 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091353,0120AG24.2% 335.9 / 117.9 198G135G (GGT→GGCpdxA4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base A (69/81);  new base G (24/24);  total (93/105)
Fisher's exact test for biased strand distribution p-value = 7.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 199 total aligned reads displayed.

GTAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACGCTCGC  >  NC_000913/52915‑53111
                                                                                                  |                                                                                                   
 tAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCg                                                                                                  >  1:3627392/1‑100 (MQ=255)
  aaaTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCgg                                                                                                 <  2:1107715/100‑1 (MQ=255)
  aaaTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCgg                                                                                                 <  2:2733505/100‑1 (MQ=255)
      gCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGCaa                                                                                              >  2:1330373/1‑99 (MQ=255)
       cgtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTaaaa                                                                                            >  1:4072405/1‑100 (MQ=255)
       cgtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTaaaa                                                                                            <  1:988432/100‑1 (MQ=255)
       cgtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTaaaa                                                                                            >  1:1703398/1‑100 (MQ=255)
       cgtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTaaaa                                                                                            >  1:1918355/1‑100 (MQ=255)
       cgtcctTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCCAAAAACTCGGTATGACCGGTaaa                                                                                             <  2:240575/99‑1 (MQ=255)
        gtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCTCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAg                                                                                           >  2:598245/1‑100 (MQ=255)
        gtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAg                                                                                           <  1:1213527/100‑1 (MQ=255)
        gtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAg                                                                                           >  1:632325/1‑100 (MQ=255)
         tcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAg                                                                                           <  2:4077307/100‑1 (MQ=255)
         tcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAgg                                                                                          <  2:2203061/100‑1 (MQ=255)
          cgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAgg                                                                                          >  1:1349376/1‑99 (MQ=255)
           gtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGaa                                                                                        >  2:3683753/1‑100 (MQ=255)
            ttGCCAGCGCCACGCGAAGTTCTTCAGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGaa                                                                                        <  2:629032/99‑1 (MQ=255)
             tGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGaaga                                                                                      >  1:4041620/1‑98 (MQ=255)
             tGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATg                                                                                      <  1:614310/100‑1 (MQ=255)
                cAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGcc                                                                                    <  2:1036770/99‑1 (MQ=255)
                 aGCGCCACGCGAAGTTCTTCTGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAg                                                                                  <  1:2865964/100‑1 (MQ=255)
                   cgcCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGcg                                                                                >  2:473147/1‑100 (MQ=255)
                   cgcCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAAATAGGTCTGGCCGGTAAAAGGaccgcaaac                                                                                 >  1:760830/1‑91 (MQ=255)
                    gcCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGcgt                                                                               >  1:2034091/1‑100 (MQ=255)
                    gcCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGcgt                                                                               >  2:3038152/1‑100 (MQ=255)
                     ccACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGcgtc                                                                              >  1:588417/1‑100 (MQ=255)
                      cACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGcgtcg                                                                             <  1:3075739/100‑1 (MQ=255)
                       aCGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGcgtcgt                                                                            <  2:1186936/100‑1 (MQ=255)
                       aCGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGcgtcgt                                                                            <  2:1828472/100‑1 (MQ=255)
                       aCGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGcgtcg                                                                             <  2:2653349/99‑1 (MQ=255)
                        cgcgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGtt                                                                           <  1:3786982/100‑1 (MQ=255)
                         gcgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTta                                                                          <  1:1189406/100‑1 (MQ=255)
                           gAAGTTCTTCGGTCGCAAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTtaat                                                                        <  1:2125870/100‑1 (MQ=255)
                              gTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAAc                                                                     <  1:2923336/100‑1 (MQ=255)
                               ttcttcGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACg                                                                    <  2:1058569/100‑1 (MQ=255)
                                  ttcGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCt                                                                 >  1:2350819/1‑100 (MQ=255)
                                  tgcGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCt                                                                 <  2:874795/98‑1 (MQ=255)
                                    cGGTCGCCAGCATCATCACCACCTTTATCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCttt                                                               >  2:940742/1‑100 (MQ=255)
                                     ggTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTa                                                              >  2:1300901/1‑100 (MQ=255)
                                     ggTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTa                                                              >  1:2775150/1‑100 (MQ=255)
                                     ggTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTa                                                              <  2:1755827/100‑1 (MQ=255)
                                     ggTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTa                                                              <  2:2754915/100‑1 (MQ=255)
                                      gTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGTAATGCCAGCGTCGTTAATAACGCCTTTan                                                             <  1:1110066/100‑2 (MQ=255)
                                      gTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTAt                                                             >  1:1618674/1‑100 (MQ=255)
                                        cGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGc                                                           >  1:3086049/1‑100 (MQ=255)
                                         nccAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCa                                                          >  2:2175189/2‑100 (MQ=255)
                                         gCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCa                                                          <  1:3643194/100‑1 (MQ=255)
                                         gCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCa                                                          <  2:3466520/100‑1 (MQ=255)
                                          ccAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGc                                                           >  1:2056948/1‑98 (MQ=255)
                                           cAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCAcc                                                        <  2:1595401/100‑1 (MQ=255)
                                           cAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCAcc                                                        >  1:55279/1‑100 (MQ=255)
                                           cAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCAcc                                                        <  1:496457/100‑1 (MQ=255)
                                              catcatCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGa                                                     <  1:3188168/100‑1 (MQ=255)
                                                tcatcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGAcc                                                   <  1:3405248/100‑1 (MQ=255)
                                                tcatcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGAcc                                                   <  1:2233607/100‑1 (MQ=255)
                                                tcatcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGAcc                                                   >  1:2297259/1‑100 (MQ=255)
                                                  atcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtg                                                 >  2:3206542/1‑100 (MQ=255)
                                                  atcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtg                                                 >  2:683836/1‑100 (MQ=255)
                                                   tcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgt                                                <  1:3248762/100‑1 (MQ=255)
                                                   tcaCCACCTTTTACGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgt                                                >  1:2715563/1‑100 (MQ=255)
                                                     accaccTTTTTCGCCTGCGAACGCTCTTCGAAAAANTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGa                                              <  1:2520394/100‑1 (MQ=255)
                                                      ccaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGAt                                             <  1:1125986/100‑1 (MQ=255)
                                                       caccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATc                                            <  2:4086950/100‑1 (MQ=255)
                                                         ccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAg                                          >  1:3493928/1‑100 (MQ=255)
                                                          cTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAgc                                         <  2:2048280/100‑1 (MQ=255)
                                                             ttttCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAgcgc                                       >  1:108724/1‑100 (MQ=255)
                                                               ttCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTGTGCACCGGACCTGTGATCAGCGCgg                                     <  1:3161748/100‑1 (MQ=255)
                                                               ttCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCgg                                     <  1:346636/100‑1 (MQ=255)
                                                               ttCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCgg                                     <  1:1052844/100‑1 (MQ=255)
                                                               ttCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCgg                                     <  2:2469474/100‑1 (MQ=255)
                                                                tCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGc                                    <  1:2953033/100‑1 (MQ=255)
                                                                 cGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCa                                   >  1:782098/1‑100 (MQ=255)
                                                                  gCCTGCGAACGGTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAGCGCCTTTATGCACCGGACCTGTGATCAGCGCgg                                     <  2:3848146/97‑1 (MQ=255)
                                                                  gCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCaa                                  >  2:3650362/1‑100 (MQ=255)
                                                                   ccTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCaaa                                 >  2:1883861/1‑100 (MQ=255)
                                                                    cgggggaCGCGCTTCGAAGAGCTCGGTAGGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGGTCAGCGCGGCAAAt                                <  1:3732734/94‑1 (MQ=255)
                                                                    cTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCa                                   <  1:3856810/97‑1 (MQ=255)
                                                                        gAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGc                            <  1:2912261/100‑1 (MQ=255)
                                                                           cGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGt                         <  1:2413617/100‑1 (MQ=255)
                                                                             ctctTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTc                       <  1:3593604/100‑1 (MQ=255)
                                                                              tctTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAGCGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCa                      <  1:1044584/100‑1 (MQ=255)
                                                                              tctTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCa                      <  1:741706/100‑1 (MQ=255)
                                                                              tctTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCa                      <  1:2498546/100‑1 (MQ=255)
                                                                               ctTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAg                     >  2:2258772/1‑100 (MQ=255)
                                                                               ctTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTAGCCGTTCAg                     >  1:2278140/1‑100 (MQ=255)
                                                                                 tCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGAc                   >  2:3622153/1‑100 (MQ=255)
                                                                                   gAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACaa                 >  2:1970769/1‑100 (MQ=255)
                                                                                    aaaaaCTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAAc                <  2:2921561/100‑1 (MQ=255)
                                                                                         cTCGGTATGGCCGGTAACAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAAcc               <  2:1501306/96‑1 (MQ=255)
                                                                                         cTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCg           <  1:2853514/100‑1 (MQ=255)
                                                                                         cTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCg           >  2:3290680/1‑100 (MQ=255)
                                                                                         cTCGGTATGACCGGGAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCg           >  2:3092941/1‑100 (MQ=255)
                                                                                          tCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGc          <  2:782092/100‑1 (MQ=255)
                                                                                            ggTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCAc        >  1:3016037/1‑100 (MQ=255)
                                                                                            ggTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCAc        >  1:3679850/1‑100 (MQ=255)
                                                                                             gTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACg       >  2:2379601/1‑100 (MQ=255)
                                                                                             gTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACg       >  2:925029/1‑100 (MQ=255)
                                                                                              tATGACCGGCAAAAGGAATCCCAGCGTCCTTACTAACGCCTGCATGTCCCTGACCAGTGCTCAGCGCTGCAAATTCGCCGCTCAGACAACCATCGCAc        >  2:3158434/1‑98 (MQ=255)
                                                                                                tGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGCTCAGCGCGCCACATTCTCCGTTCAGACAACCATCGccc        >  2:1742351/1‑94 (MQ=255)
                                                                                                tGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACGCTc    >  2:1193035/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
GTAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACGCTCGC  >  NC_000913/52915‑53111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: