Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,632,584 A→T 17.2% intergenic (‑49/+20) guaA ← / ← guaB GMP synthetase (glutamine aminotransferase)/IMP dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,632,5840AT17.2% 367.6 / 65.1 151intergenic (‑49/+20)guaA/guaBGMP synthetase (glutamine aminotransferase)/IMP dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (60/65);  new base T (11/15);  total (71/80)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 153 total aligned reads displayed.

AGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGC  >  NC_000913/2632485‑2632683
                                                                                                   |                                                                                                   
 gaACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACAt                                                                                                    >  1:1437623/1‑100 (MQ=255)
   aCCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGa                                                                                                  <  2:647773/100‑1 (MQ=255)
   aCCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGa                                                                                                  >  2:3401367/1‑100 (MQ=255)
    ccGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGaa                                                                                                 >  2:1404942/1‑100 (MQ=255)
     cGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAg                                                                                                <  2:2778152/100‑1 (MQ=255)
      gAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGt                                                                                               >  2:3707996/1‑100 (MQ=255)
       aaGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTc                                                                                              <  1:2222541/100‑1 (MQ=255)
         gTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCgg                                                                                            >  1:1515450/1‑100 (MQ=255)
         gTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCgg                                                                                            <  1:3125723/100‑1 (MQ=255)
         gTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCgg                                                                                            <  1:1231790/100‑1 (MQ=255)
         gTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCACTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCgg                                                                                            >  1:1016424/1‑100 (MQ=255)
          tCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCggg                                                                                           <  2:2832433/100‑1 (MQ=255)
           ccAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGc                                                                                          <  1:454742/100‑1 (MQ=255)
           ccAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGc                                                                                          <  2:2015745/100‑1 (MQ=255)
           ccAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGc                                                                                          <  1:886219/100‑1 (MQ=255)
           ccAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGc                                                                                          <  2:5179/100‑1 (MQ=255)
           ccAGAAGGAGGATGCGATGCTTATGAATGTGTTCCGTCATGGACGCGTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGc                                                                                          <  1:3432406/100‑1 (MQ=255)
            cAGAATGAGGATGCGATGCGTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGCg                                                                                         <  2:3532765/100‑1 (MQ=255)
             aGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGCGa                                                                                        <  1:1864719/100‑1 (MQ=255)
              gAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGCGat                                                                                       <  1:3509889/100‑2 (MQ=255)
              gAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGCGat                                                                                       <  2:2412580/100‑2 (MQ=255)
              gAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGCGa                                                                                        <  2:307660/99‑1 (MQ=255)
               aaTGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAag                                                                                      <  1:2925493/100‑1 (MQ=255)
                aTGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAaga                                                                                     <  1:82105/100‑1 (MQ=255)
                 ttaGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGAGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGCGattta                                                                                    <  2:3125014/98‑5 (MQ=38)
                 tgatggtgctatgcTTATGAAGGTGTTCCGTAAGTGACGCGTATTCCGAGGGAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGCGa                                                                                        <  2:2029706/90‑1 (MQ=255)
                   aGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGaa                                                                                  >  1:2079402/1‑100 (MQ=255)
                   aGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGaa                                                                                  <  1:3618789/100‑1 (MQ=255)
                   aGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGaa                                                                                  >  1:3322685/1‑100 (MQ=255)
                    ggATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAAt                                                                                 >  2:2701168/1‑100 (MQ=255)
                     gatgcgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCGGGCGatttatatt                                                                                <  1:573670/100‑9 (MQ=37)
                     gatgcgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATc                                                                                <  2:4127535/100‑1 (MQ=255)
                      atgcgatgcTTATGAATGTTTTCCGTCATTGACGCTTATCCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCa                                                                               <  2:2771394/100‑1 (MQ=255)
                        gcgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGTCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAgg                                                                             <  2:2636063/100‑1 (MQ=255)
                         cgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGa                                                                            >  1:1040129/1‑100 (MQ=255)
                         cgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGa                                                                            <  1:3580865/100‑1 (MQ=255)
                         cgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGa                                                                            >  1:1332336/1‑100 (MQ=255)
                          gatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAg                                                                           >  1:1617497/1‑100 (MQ=255)
                           atgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGc                                                                          <  1:1950248/100‑1 (MQ=255)
                             gcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGccc                                                                        <  1:3396378/100‑1 (MQ=255)
                              cTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGTAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCa                                                                       >  2:3862015/1‑100 (MQ=255)
                              cTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCa                                                                       <  1:3181758/100‑1 (MQ=255)
                              cTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCa                                                                       <  2:3253872/100‑1 (MQ=255)
                               ttATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAg                                                                      >  1:3336037/1‑100 (MQ=255)
                                 aTGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGAc                                                                    <  1:3284673/100‑1 (MQ=255)
                                 aTGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGAc                                                                    <  2:2232309/100‑1 (MQ=255)
                                  tGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCACGACATGAAGTCGGGAGAAGAGAATCAGGAGCCCAGACg                                                                   >  2:1330952/1‑100 (MQ=255)
                                  tGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGACCGACATGAAGTCGGGCGAAGAGAATCAGGAGCACAGACg                                                                   >  2:3589069/1‑100 (MQ=255)
                                       gTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGt                                                              <  1:2426384/100‑1 (MQ=255)
                                            ccGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCggg                                                         <  1:2928606/100‑1 (MQ=255)
                                            ccGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCggg                                                         >  1:969689/1‑100 (MQ=255)
                                            ccGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCggg                                                         >  2:1293137/1‑100 (MQ=255)
                                             cGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCgggg                                                        <  1:1194490/100‑1 (MQ=255)
                                             cGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCgggg                                                        >  2:1507802/1‑100 (MQ=255)
                                               tCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGAc                                                      <  1:2200720/100‑1 (MQ=255)
                                                cATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGAGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGAct                                                     >  1:3212107/1‑100 (MQ=255)
                                                 aTTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGActc                                                    >  1:698190/1‑100 (MQ=255)
                                                 aTTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAAAATCAGGAGCCCAGACGGTAGTTCGGGGActc                                                    >  2:2460705/1‑100 (MQ=255)
                                                    gACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCttt                                                 <  1:1159673/100‑1 (MQ=255)
                                                    gACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCttt                                                 <  2:3427639/100‑1 (MQ=255)
                                                      cGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAg                                               <  2:2830740/100‑1 (MQ=255)
                                                        cTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTa                                             >  1:2120222/1‑100 (MQ=255)
                                                           aTTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATg                                          <  1:307231/100‑1 (MQ=255)
                                                            ttCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATgg                                         <  2:3093735/100‑1 (MQ=255)
                                                            ttCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATgg                                         >  2:1661499/1‑100 (MQ=255)
                                                              ccGAGGCAAGTGAAACAGATAATATAAATCGCTCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTc                                       >  1:3921132/1‑100 (MQ=255)
                                                              ccGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTc                                       >  1:2568569/1‑100 (MQ=255)
                                                               cGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCa                                      >  2:403926/1‑100 (MQ=255)
                                                                    cAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCAcgtcg                                 >  2:1174756/1‑100 (MQ=255)
                                                                     aaGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCAcgtcgt                                >  1:1741259/1‑100 (MQ=255)
                                                                       gTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGa                              <  1:3468293/100‑1 (MQ=255)
                                                                         gAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAAc                            >  2:130805/1‑100 (MQ=255)
                                                                         gAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAAc                            >  2:1455333/1‑100 (MQ=255)
                                                                           aaCAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGt                          >  1:1488618/1‑100 (MQ=255)
                                                                             cAGATAATATAAACAGCCCAACATGAAGACGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGAGACTCTTTAGTAATGGTCAAGTCGTGAACGt                          <  2:3714680/98‑1 (MQ=255)
                                                                              aGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAGTCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGc                       <  1:3743887/100‑1 (MQ=255)
                                                                               gATAATATAAATCGCCCGACATGAAGTCGGGAGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTATTTAGTAATGGTCACGTCGTGAACGTGGCt                      >  1:167379/1‑100 (MQ=255)
                                                                                 taataTAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCttt                    >  2:3589329/1‑100 (MQ=255)
                                                                                 taataTAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCttt                    <  2:1349821/100‑1 (MQ=255)
                                                                                   atatAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTcc                  >  2:1238397/1‑100 (MQ=255)
                                                                                    tataAATCGCTCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTATAGTAATGGTCACGTCGTGAACGTGGCTTTCCt                 <  2:2714365/100‑1 (MQ=255)
                                                                                    tataAATCGCCCGACATGAGGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCt                 <  1:1524546/100‑1 (MQ=255)
                                                                                     ataAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTg                <  1:439552/100‑1 (MQ=255)
                                                                                       tcttCGCCCGACTTCCTGTCGGGCGCAGAGAATCAGGAGCCCAGACTGTAGTTCGGGGACTCTTTAGTAATGGTCACCTCGTTAACGTGGCTTTCCt                 >  2:1045232/4‑97 (MQ=255)
                                                                                       tcttCGCCCGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGaa              >  1:1577383/4‑100 (MQ=38)
                                                                                       caaTCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGaa              <  1:362451/99‑1 (MQ=255)
                                                                                       aaaTCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCtca               >  2:1411181/1‑97 (MQ=255)
                                                                                       aaaTCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGaa              <  1:186048/100‑1 (MQ=255)
                                                                                       aaaTCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGaa              <  2:2289600/100‑1 (MQ=255)
                                                                                         ttCGCCCGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATg            >  1:2867346/2‑100 (MQ=255)
                                                                                         ttCGCCCGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATg            >  1:2867606/2‑100 (MQ=255)
                                                                                         ttCGCCCGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATg            >  2:2134179/2‑100 (MQ=255)
                                                                                         ttCGCCCGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATg            >  1:336556/2‑100 (MQ=255)
                                                                                         aTCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGATTCTTGAGTAATGTTCACTTCGTGAACTTGGCTTTCCTGAATg            >  1:3875914/1‑100 (MQ=255)
                                                                                         aTCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATg            <  2:1898254/100‑1 (MQ=255)
                                                                                           cGCCCGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGcc          >  1:2710580/1‑100 (MQ=255)
                                                                                           cGCCCGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGcc          >  1:2732828/1‑100 (MQ=255)
                                                                                           cGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGcc          <  2:1880361/100‑1 (MQ=255)
                                                                                             cccGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCg        <  1:3695977/100‑1 (MQ=255)
                                                                                                   aTGAAGTCGGGGGAAGAGAATCAGGAGCCCAGACGGTAGGTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGc  <  2:1016343/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
AGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGC  >  NC_000913/2632485‑2632683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: