Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,634,176 A→G 16.6% intergenic (‑106/‑56) guaB ← / → xseA IMP dehydrogenase/exonuclease VII, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,634,1760AG16.6% 397.0 / 64.2 180intergenic (‑106/‑56)guaB/xseAIMP dehydrogenase/exonuclease VII, large subunit
Reads supporting (aligned to +/- strand):  ref base A (74/76);  new base G (13/17);  total (87/93)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

TCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGCAATTTTTACCGTTAGTCGCCT  >  NC_000913/2634079‑2634275
                                                                                                 |                                                                                                   
tctcGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTTCAAAAATTGCTTGCTATCCCCGAAgg                                                                                                   >  2:3452015/1‑100 (MQ=255)
   cGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCg                                                                                                <  2:3735490/100‑1 (MQ=255)
    gACGAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACACCATATTGAAAAAAATGCTTGCTATCCCCGAAGGGCg                                                                                                <  2:3196875/99‑1 (MQ=255)
     aCCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAGCCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCggg                                                                                              <  2:3017126/100‑1 (MQ=255)
        agagTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCTGGtt                                                                                            >  2:2846534/1‑99 (MQ=255)
         gagTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAGGGGCGGGTTa                                                                                           <  1:870827/99‑1 (MQ=255)
         gagTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTAc                                                                                          <  1:190251/100‑1 (MQ=255)
           gTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTTCTTTTTTTTTTTGCTATCCCCGAAGGGCGGGTTACt                                                                                         >  2:1097174/1‑99 (MQ=255)
           gTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTa                                                                                        >  1:2373159/1‑100 (MQ=255)
           gTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTa                                                                                        >  1:2493730/1‑100 (MQ=255)
           gTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTa                                                                                        >  1:1168766/1‑100 (MQ=255)
            tGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTGCCCCGTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTAt                                                                                       <  2:2441804/100‑1 (MQ=255)
             ggTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATc                                                                                      <  2:1168759/100‑1 (MQ=255)
             ggTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATc                                                                                      >  1:1679213/1‑100 (MQ=255)
              gTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCg                                                                                     <  1:624694/100‑1 (MQ=255)
               ttAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGa                                                                                    >  2:595736/1‑100 (MQ=255)
                taataaATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGAc                                                                                   >  1:3638643/1‑100 (MQ=255)
                  ataaataTTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTg                                                                                 <  1:1015396/100‑1 (MQ=255)
                  ataaataTTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTAGCCCCGAGGGGCGGGTTACTATCGACTg                                                                                 <  1:3585013/100‑1 (MQ=255)
                   taaataTTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGa                                                                                >  2:1819468/1‑100 (MQ=255)
                   taaataTTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGa                                                                                >  1:1068731/1‑100 (MQ=255)
                    aaataTTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGaa                                                                               <  2:1814335/100‑1 (MQ=255)
                      atatTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATa                                                                             >  1:1704618/1‑100 (MQ=255)
                       tatTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATaa                                                                            <  1:437238/100‑1 (MQ=255)
                        atTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAGGGGCGGGTTACTATCGACTGAATAAc                                                                           <  1:1379786/100‑1 (MQ=255)
                        atTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAAc                                                                           <  2:3651346/100‑1 (MQ=255)
                        atTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAAc                                                                           <  2:3779029/100‑1 (MQ=255)
                          tGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACct                                                                         <  1:3351291/100‑1 (MQ=255)
                           gCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAGGGGCGGGTTACTATCGACTGAATAACctg                                                                        >  1:2345110/1‑100 (MQ=255)
                           gCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACctg                                                                        <  1:1228434/100‑1 (MQ=255)
                             cgcgGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACctgc                                                                       <  1:1510445/99‑1 (MQ=255)
                                ggCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGAt                                                                   >  2:3045814/1‑100 (MQ=255)
                                 gCATTTTACAAATCTTAACCGCTTTCTTCTACCCATTTGATCAAAAAAGACTTACTACCCCCGAAGTCCGGTTTACTATCGACTGAATAAc                                                                           <  2:3647898/91‑1 (MQ=255)
                                 gCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGAtt                                                                  <  2:1515663/100‑1 (MQ=255)
                                  cATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGAttt                                                                 <  1:2132426/100‑1 (MQ=255)
                                   aTTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTTCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTa                                                                >  2:480500/1‑100 (MQ=255)
                                   aTTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTa                                                                <  1:1316783/100‑1 (MQ=255)
                                    ttATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAGGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAg                                                               <  2:3575647/100‑1 (MQ=255)
                                     tataCAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGa                                                              >  1:1002643/1‑100 (MQ=255)
                                     tataCAGAGCGTAACCGATTGCATCTAAACAAAAAAAAAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTa                                                                <  2:1270828/98‑1 (MQ=255)
                                         cAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAGGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAAttt                                                          <  1:3413693/100‑1 (MQ=255)
                                         cAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAAttt                                                          >  1:2263891/1‑100 (MQ=255)
                                          agagCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAGGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTg                                                         <  1:255106/100‑1 (MQ=255)
                                           gagCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAGGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGa                                                        <  1:3194198/100‑1 (MQ=255)
                                           gagCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGa                                                        <  2:3244962/100‑1 (MQ=255)
                                            agCGTACCCCATTGCATCTACCCCTTTTTACAAAAAATGCTTGCCATCCCCGAGGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGAt                                                       <  2:1848114/100‑1 (MQ=255)
                                            agCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAGGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGAt                                                       <  1:2646465/100‑1 (MQ=255)
                                             gCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGAtc                                                      <  1:2943592/100‑1 (MQ=255)
                                             gCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGAtc                                                      <  1:4046196/100‑1 (MQ=255)
                                                tAACCGACTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGAtct                                                     <  2:2917389/98‑1 (MQ=255)
                                                 naCCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCg                                                   >  1:3706179/2‑99 (MQ=255)
                                                 aaCCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGc                                                  <  1:2421578/100‑1 (MQ=255)
                                                  aCCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCt                                                 >  1:3760331/1‑100 (MQ=255)
                                                  aCCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCt                                                 >  1:3301163/1‑100 (MQ=255)
                                                   ccGATTGCATCTACCCCTTTTTTTTTTTTTTTCTTGCTATCCCCGAAAGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTc                                                >  2:28364/1‑100 (MQ=255)
                                                   ccGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGc                                                  <  2:501580/98‑1 (MQ=255)
                                                   ccGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTc                                                <  1:973657/100‑1 (MQ=255)
                                                   ccGATTGCATCTACACCATTATGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCt                                                 <  2:2308495/99‑1 (MQ=255)
                                                      aTTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTcaca                                             >  1:3610560/1‑100 (MQ=255)
                                                       ttGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAGGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACAt                                            <  1:827058/100‑1 (MQ=255)
                                                         gCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGt                                          >  1:1831897/1‑100 (MQ=255)
                                                           aTCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAGGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTa                                        >  2:2803688/1‑100 (MQ=255)
                                                            tCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTAc                                       <  1:2775587/100‑1 (MQ=255)
                                                            tCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTAc                                       >  1:296141/1‑100 (MQ=255)
                                                            tCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTAc                                       <  1:711788/100‑1 (MQ=255)
                                                              tACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCt                                     <  1:3753588/100‑1 (MQ=255)
                                                                 cccTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTtct                                  <  2:3879133/100‑1 (MQ=255)
                                                                  ccTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTtctc                                 >  1:557907/1‑100 (MQ=255)
                                                                   cTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCa                                <  2:3149590/100‑1 (MQ=255)
                                                                     ttttGCAAAAACTGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAAt                              >  1:1714636/1‑100 (MQ=255)
                                                                      tttGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATc                             <  1:1924169/100‑1 (MQ=255)
                                                                        tGCACAAAATGCTTGCTATCCCCGCATGGCGGGTTTCTATCTTCTGTATATATTGCTGATGTAGAATTTAATAACGCTCTCATGTTAcccgctc                                 <  2:2929821/94‑6 (MQ=255)
                                                                         gCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATcccc                          >  2:1348680/1‑100 (MQ=255)
                                                                         gCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCCGCTGATTTAGACTTTGATCTCGCTCACATGTTACCTTCTCAATcccc                          >  2:2221849/1‑100 (MQ=255)
                                                                          cAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCt                         <  2:2890380/100‑1 (MQ=255)
                                                                          cAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCt                         >  2:789098/1‑100 (MQ=255)
                                                                          cAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCt                         >  2:148879/1‑100 (MQ=255)
                                                                          cAAAAAATGCTTGCTATCCCCGAAGGGCGGGCTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCt                         >  1:528752/1‑100 (MQ=255)
                                                                           aaaaaaTGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATACCCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTg                        <  1:3506293/100‑1 (MQ=255)
                                                                           aaaaaaTGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTg                        >  1:1606780/1‑100 (MQ=255)
                                                                            aaaaaTGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGc                       >  2:3493258/1‑100 (MQ=255)
                                                                            aaaaaTGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGc                       <  2:1714624/100‑1 (MQ=255)
                                                                            aaaaaTGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGc                       <  2:1344968/100‑1 (MQ=255)
                                                                               aaTGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGCAAt                    >  1:278609/1‑100 (MQ=255)
                                                                                 tgcttgctATCCCCGAAGGGCGGGTTACTAACGTCTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCGCCTGCAAttt                  >  2:2715424/1‑100 (MQ=255)
                                                                                      gctATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTATCTTCTCAATCCCCTGCAATTTTTAcc             <  1:2509647/100‑1 (MQ=255)
                                                                                      gctATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGCAATTTTTAcc             >  2:2383815/1‑100 (MQ=255)
                                                                                      gctATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGCAATTTTTAcc             <  2:2117899/100‑1 (MQ=255)
                                                                                      gctATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGCAATTTTTAcc             <  1:3848147/100‑1 (MQ=255)
                                                                                        tATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGCAATTTTTACCGt           <  2:2174874/100‑1 (MQ=255)
                                                                                        tATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGCAATTTTTACCGt           >  2:4039117/1‑100 (MQ=255)
                                                                                         aTCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGCAATTTTTACCGtt          <  1:1117678/100‑1 (MQ=255)
                                                                                          tCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGCAATTTTTACCGTTa         >  1:3081072/1‑100 (MQ=255)
                                                                                          tCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGCAATTTTTACCGTTa         >  2:2969890/1‑100 (MQ=255)
                                                                                           ccccGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGCAATTTTTACCGTTAg        >  1:2161642/1‑100 (MQ=255)
                                                                                           ccccGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGCAATTTTTACCGTTAg        <  2:1054452/100‑1 (MQ=255)
                                                                                              cGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGCAATTTTTACCGTTAGTCg     <  2:1400737/100‑1 (MQ=255)
                                                                                                aaGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGCAATTTTTACCGTTAGTCGcc   <  2:1741230/100‑1 (MQ=255)
                                                                                                aaGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGCAATTTTTACCGTTAGTCGcc   <  1:2348923/100‑1 (MQ=255)
                                                                                                 aGGGCGGGTTACTATCGACTGAATAACCTGCTGTTTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGCAATTTTTACCGTTAGTCGCCt  >  2:2690362/1‑100 (MQ=255)
                                                                                                 |                                                                                                   
TCTCGACCAGAGTGGTTAATAAATATTGCCGCGGCATTATACAGAGCGTAACCGATTGCATCTACCCCTTTTTGCAAAAAATGCTTGCTATCCCCGAAGGGCGGGTTACTATCGACTGAATAACCTGCTGATTTAGAATTTGATCTCGCTCACATGTTACCTTCTCAATCCCCTGCAATTTTTACCGTTAGTCGCCT  >  NC_000913/2634079‑2634275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: