Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,638,037 C→A 23.7% A206S (GCG→TCG)  bamB ← BamABCDE complex OM biogenesis lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,638,0370CA23.7% 391.7 / 113.1 185A206S (GCG→TCG) bamBBamABCDE complex OM biogenesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (80/61);  new base A (20/24);  total (100/85)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 185 total aligned reads displayed.

GAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCG  >  NC_000913/2637940‑2638135
                                                                                                 |                                                                                                  
gAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAAc                                                                                                  <  1:953960/100‑1 (MQ=255)
      gTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAg                                                                                            <  1:860936/100‑1 (MQ=255)
      gTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAg                                                                                            <  2:2345767/100‑1 (MQ=255)
       tCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGc                                                                                           <  1:577323/100‑1 (MQ=255)
         gCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCg                                                                                         <  2:3174864/100‑1 (MQ=255)
            tGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTg                                                                                      >  1:3063104/1‑100 (MQ=255)
            tGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTg                                                                                      <  1:3543517/100‑1 (MQ=255)
             gggAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGt                                                                                     >  2:3367189/1‑100 (MQ=255)
               gAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCg                                                                                   >  2:1614928/1‑100 (MQ=255)
               gAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCg                                                                                   >  1:1874499/1‑100 (MQ=255)
                 aaTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGgc                                                                                 <  2:3271288/100‑1 (MQ=255)
                  aTACGCTGCTGCCACATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGgcg                                                                                <  2:2821997/100‑1 (MQ=255)
                  aTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGgcg                                                                                >  1:3183874/1‑100 (MQ=255)
                      gctgctGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGa                                                                            >  1:1284160/1‑100 (MQ=255)
                       ctgctgCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGAc                                                                           >  1:511633/1‑100 (MQ=255)
                         gctgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTc                                                                         >  1:3391128/1‑100 (MQ=255)
                          ctgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCg                                                                        <  1:224505/100‑1 (MQ=255)
                           tgcCAAATCATCTGGCCCTGTTCCCTCAGCGCTGCGCTGACGCGACCATTATCTCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGc                                                                       >  2:3702349/1‑100 (MQ=255)
                           tgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGc                                                                       >  1:1045524/1‑100 (MQ=255)
                           tgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGc                                                                       >  2:2859666/1‑100 (MQ=255)
                           tgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGc                                                                       >  2:477608/1‑100 (MQ=255)
                           tgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCCACCATTATCGCCCCCCACGACGGCCGCACCACAAGCCGCTGCCgcc                                                                                 >  2:3393243/1‑88 (MQ=255)
                           tgcCAAATCATCTGGCCCTGTCCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCa                                                                              <  2:1651382/93‑1 (MQ=255)
                            gcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGcc                                                                      >  1:1974000/1‑100 (MQ=255)
                            gcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGcc                                                                      >  2:2723696/1‑100 (MQ=255)
                            gcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACCCGcc                                                                      >  1:1976094/1‑100 (MQ=255)
                            gcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGcc                                                                      <  2:2257931/100‑1 (MQ=255)
                                aaTCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGc                                                                  >  2:3876771/1‑100 (MQ=255)
                                aaTCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGc                                                                  >  2:1043042/1‑100 (MQ=255)
                                 atcatcTGGTCCTGTTCCATCAGCACTGCGCTGACGCGACGATTCTCGCCCTTCAAGACAGCCGCACCAAAAGCCGTTGTTGGCTCAGACTCGGCACGCa                                                                 >  2:1022152/1‑100 (MQ=255)
                                   catcTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCaaa                                                               <  1:2338274/100‑1 (MQ=255)
                                    atcTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAag                                                              >  2:2437331/1‑100 (MQ=255)
                                     tatGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAaga                                                             >  2:670987/3‑100 (MQ=255)
                                      cTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagag                                                            >  1:810436/1‑100 (MQ=255)
                                      cTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagag                                                            <  1:2847156/100‑1 (MQ=255)
                                       tGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagaga                                                           <  1:1032962/100‑1 (MQ=255)
                                       tGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagaga                                                           >  1:2974430/1‑100 (MQ=255)
                                       tGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagaga                                                           >  1:150269/1‑100 (MQ=255)
                                        ggCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagagag                                                          >  2:3461352/1‑100 (MQ=255)
                                        ggCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAagagag                                                          >  1:3904462/1‑100 (MQ=255)
                                         gCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGc                                                         <  1:1612041/100‑1 (MQ=255)
                                         gCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGc                                                         <  2:121195/100‑1 (MQ=255)
                                         gCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCACCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGc                                                         <  2:1115134/100‑1 (MQ=255)
                                            cTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGaa                                                      >  1:1378674/1‑100 (MQ=255)
                                             tGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAg                                                     >  1:3957589/1‑100 (MQ=255)
                                             tGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAg                                                     <  2:3904436/100‑1 (MQ=255)
                                             tGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAg                                                     >  1:680456/1‑100 (MQ=255)
                                              gTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGCCGGCCGCACCAAACGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAgg                                                    >  2:3652266/1‑100 (MQ=255)
                                                tCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCa                                                  >  2:855023/1‑100 (MQ=255)
                                                tCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCa                                                  >  1:649637/1‑100 (MQ=255)
                                                tCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCa                                                  <  2:495331/100‑1 (MQ=255)
                                                 ccATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCCAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCa                                                  >  1:249750/1‑99 (MQ=255)
                                                 ccATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGGGCGAAGGCa                                                  >  1:2480160/1‑99 (MQ=255)
                                                  cATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCata                                                <  2:3235136/100‑1 (MQ=255)
                                                      aGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGa                                            >  2:3433632/1‑100 (MQ=255)
                                                      aGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCCGCGCAGACTCGCCACGCCAAGAGAGCGAAGCCATATCGa                                            >  2:1480843/1‑100 (MQ=255)
                                                        cACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAgg                                          <  1:2752754/100‑1 (MQ=255)
                                                        cACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAgg                                          <  1:1570786/100‑1 (MQ=255)
                                                          cTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGtt                                        <  1:3951892/100‑1 (MQ=255)
                                                             cgcTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAAc                                     >  1:3224866/1‑100 (MQ=255)
                                                             cgcTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAAc                                     <  1:3524336/100‑1 (MQ=255)
                                                             cgcTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAAc                                     <  1:2682149/100‑1 (MQ=255)
                                                               cTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTg                                   <  1:41046/100‑1 (MQ=255)
                                                               cTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTg                                   <  1:40345/100‑1 (MQ=255)
                                                               cTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTg                                   <  1:2544106/100‑1 (MQ=255)
                                                                tGACGCGACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGATAACTg                                   >  2:2220163/1‑99 (MQ=255)
                                                                   cgcgACCAGTATCTCCCCCCACGCCGGCCGCACCAAAAGCCGATGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTg                                   <  2:2534723/96‑1 (MQ=255)
                                                                     cgACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCAtt                             <  2:1906028/100‑1 (MQ=255)
                                                                     cgACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCAtt                             >  2:1097006/1‑100 (MQ=255)
                                                                      gACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCAttt                            <  2:891477/100‑1 (MQ=255)
                                                                      gACCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAACGAGAGCGAAGGCATATCGAGGTTAACTGTCCAttt                            >  1:2822139/1‑100 (MQ=255)
                                                                       aCCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGTCTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTg                           <  2:1062058/100‑1 (MQ=255)
                                                                       aCCATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTg                           <  1:2066461/100‑1 (MQ=255)
                                                                        ccATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGCCTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTACCTGTCCATTTg                           >  1:1769741/1‑99 (MQ=255)
                                                                        ccATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGa                          >  1:3381667/1‑100 (MQ=255)
                                                                        ccATTATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTg                           <  1:15386/99‑1 (MQ=255)
                                                                         cATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGAc                         >  2:1610450/1‑100 (MQ=255)
                                                                          aTTATCGCGCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAACGAGAGCGAAGGCATATCGAGGCTAACTGTCCATTTGACa                        >  2:4093011/1‑100 (MQ=255)
                                                                           ttATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAg                       <  2:2136004/100‑1 (MQ=255)
                                                                           ttATCGCCCCCCACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAg                       <  1:758942/100‑1 (MQ=255)
                                                                              tCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAgcgc                    >  1:1353518/1‑100 (MQ=255)
                                                                                ttccgatctgacgGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCg                  <  2:1741273/91‑1 (MQ=255)
                                                                                gCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCg                  >  2:2748578/1‑100 (MQ=255)
                                                                                gCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCg                  >  1:2100716/1‑100 (MQ=255)
                                                                                gCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCg                  >  2:4123998/1‑100 (MQ=255)
                                                                                 ccccccACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGt                 <  2:3977025/100‑1 (MQ=255)
                                                                                    cccACGACGGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAg              >  1:2378209/1‑100 (MQ=255)
                                                                                     ccACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGc             <  1:1178977/100‑1 (MQ=255)
                                                                                     ccACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGc             <  2:1535689/100‑1 (MQ=255)
                                                                                      cACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCt            >  1:2933147/1‑100 (MQ=255)
                                                                                         gacgGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCg         <  1:1099057/100‑1 (MQ=255)
                                                                                         gacgGCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCg         <  2:31558/100‑1 (MQ=255)
                                                                                            ggCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTc      <  2:95667/100‑1 (MQ=255)
                                                                                             gCCGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCa     >  1:3631693/1‑100 (MQ=255)
                                                                                             gCCGAACCAAAAGCCGGTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGt        <  2:2637532/97‑1 (MQ=255)
                                                                                              ccGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGCGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAg    >  2:792513/1‑100 (MQ=255)
                                                                                              ccGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAg    <  2:3703283/100‑1 (MQ=255)
                                                                                              ccGAACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAg    >  1:3696074/1‑100 (MQ=255)
                                                                                               cGCACCAACAGCCGTTGTCGGCGAACACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGGCCATTTGACAGCGCCGTCAGCTTCGTTCAgc   <  2:3339718/100‑1 (MQ=255)
                                                                                                gCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAgcg  <  1:2537424/100‑1 (MQ=255)
                                                                                                 |                                                                                                  
GAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCG  >  NC_000913/2637940‑2638135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑