BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,892,993	G→A	56.8%	E113K (GAA→AAA)	ygcN →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,892,993	0	G	A	56.8%	60.2 / 196.4	161	E113K (GAA→AAA)	ygcN	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (40/29); new base A (36/55); total (77/84)
Fisher's exact test for biased strand distribution p-value = 2.55e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 175 total aligned reads displayed.

CCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGATATTCTC  >  NC_000913/2892898‑2893091

ccGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAaaaa                                                                                                <  1:1706749/100‑1 (MQ=255)

ccGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAaaaa                                                                                                >  2:2435444/1‑100 (MQ=255)

 cGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCaaaaaa                                                                                               >  1:2428329/1‑100 (MQ=255)

     ggCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGCAGCCGAAAaagaag                                                                                           >  1:3783588/1‑100 (MQ=255)

     ggCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAaagaag                                                                                           <  2:157549/100‑1 (MQ=255)

     ggCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAaagaag                                                                                           <  1:2740873/100‑1 (MQ=255)

       cGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGgt                                                                                         >  1:3917901/1‑100 (MQ=255)

       cGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGgt                                                                                         >  1:3547552/1‑100 (MQ=255)

          aaCGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTc                                                                                      <  2:244386/100‑1 (MQ=255)

           acgacgTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGgtgtag                                                                                     >  2:2497393/1‑98 (MQ=255)

           acgacgTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCg                                                                                     <  2:3973494/100‑1 (MQ=255)

            cgacgTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGa                                                                                    >  1:704527/1‑100 (MQ=255)

             gacgTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGaa                                                                                   >  1:3226282/1‑100 (MQ=255)

               cgTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATg                                                                                 <  1:1554547/100‑1 (MQ=255)

               cgTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATg                                                                                 >  1:1678292/1‑100 (MQ=255)

               cgTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATg                                                                                 >  2:782806/1‑100 (MQ=255)

               cgTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATg                                                                                 <  2:3430165/100‑1 (MQ=255)

                  tttCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCAt                                                                              >  1:1895116/1‑100 (MQ=255)

                  tttCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCAt                                                                              <  1:1055587/100‑1 (MQ=255)

                   ttCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATc                                                                             <  1:2212487/100‑1 (MQ=255)

                     ccAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATccc                                                                           <  1:2593895/100‑1 (MQ=255)

                        gatgaCAGCCCGGCGGTAAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCAAAAAAAAAAAAAGAAGGTGTCGAATGCATCCCCg                                                                         <  2:2462065/95‑1 (MQ=255)

                        gCTTACAGCCCGGCGGTGAATCCTGGAGTTTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGTTGTCGAATGCATCCCCgg                                                                        >  1:4114792/1‑100 (MQ=255)

                         cTTACAGCCCGGCGGTGAATCCTGGCGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGa                                                                       >  2:2018507/1‑100 (MQ=255)

                          ttACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAg                                                                      <  1:1352795/100‑1 (MQ=255)

                           tACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGc                                                                     >  1:4023110/1‑100 (MQ=255)

                           tACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGc                                                                     <  2:332625/100‑1 (MQ=255)

                             cAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGa                                                                   >  2:1868317/1‑100 (MQ=255)

                             cAGCCCGGCGGTGAATCCCGGAGTGTATTACCTCCACGGTTCGATTCGTTGGTGGTTGCCGAAGCCAAAAAAGGGGGTGTCGAATGCATGCCCGGAGCGa                                                                   >  2:1781245/1‑100 (MQ=255)

                               gCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACg                                                                 >  1:1234429/1‑100 (MQ=255)

                               gCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACg                                                                 >  1:3443671/1‑100 (MQ=255)

                                cccGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACgg                                                                >  1:3430058/1‑100 (MQ=255)

                                cccGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACgg                                                                >  1:2111470/1‑100 (MQ=255)

                                  cggcggTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTg                                                              <  1:921790/100‑1 (MQ=255)

                                   gggggTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTg                                                              <  2:378755/96‑1 (MQ=255)

                                    gcggTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGa                                                            <  1:988694/100‑1 (MQ=255)

                                      ggTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATg                                                          >  1:3057742/1‑100 (MQ=255)

                                       gTGAGTCCTGGAGTGTATGACGTGCACGATTCGATCCTTGGCGGGTTGCCGAAGCCATAAAAGAAGGTGTCTAATGCATGCCCGGAGCGACTGTGGATGc                                                         >  2:1845699/1‑100 (MQ=255)

                                       gTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGc                                                         >  1:2469551/1‑100 (MQ=255)

                                       gTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGc                                                         <  1:2948879/100‑1 (MQ=255)

                                         gAATCCTGGATTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCAc                                                       <  2:4061787/100‑1 (MQ=255)

                                          aaTCCTGGAGTGTATTACGTGCACGATTCGATCCGGGGCTGGTTGCCGAAGCCGAAAAATAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACt                                                      <  1:1980403/100‑1 (MQ=255)

                                           aTCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTg                                                     >  2:3965161/1‑100 (MQ=255)

                                            tCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGt                                                    >  2:3374627/1‑100 (MQ=255)

                                             ccTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTa                                                   <  2:1866981/100‑1 (MQ=255)

                                              ntGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTAt                                                  >  2:2599115/2‑100 (MQ=255)

                                              cTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTAt                                                  <  2:934232/100‑1 (MQ=255)

                                              cTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTAt                                                  >  2:114900/1‑100 (MQ=255)

                                              cTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTAt                                                  >  2:3361399/1‑100 (MQ=255)

                                               tGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATg                                                 <  1:95969/100‑1 (MQ=255)

                                                ggAGTGTATTACGGGCACGATTCGATCCGTGGCTGGTTGACGAAGCCGAAAAAGGAGGGGTCGAATGCAGCACCGGAGCGACGGGGGATGCACTGTAt                                                  >  2:3888522/1‑98 (MQ=255)

                                                 gAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATgaa                                               <  1:2127333/100‑1 (MQ=255)

                                                   gtgtATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGAGGATGCACTGTATgaaga                                             >  2:803937/1‑100 (MQ=255)

                                                   gtgtATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATgaaga                                             <  1:743314/100‑1 (MQ=255)

                                                   gtgtATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATgaaga                                             <  1:3655059/100‑1 (MQ=255)

                                                   gtgtATTACGTGCACGATTCGATACGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATgaaga                                             >  1:550402/1‑100 (MQ=255)

                                                       aTTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAAc                                         <  1:2407604/100‑1 (MQ=255)

                                                         tACGTGCACGATTCGATCCGTGGCTGGTTGCCGACGCCTAATAAGCAGGTGTCGAGTGTATCGCCGGAGCGACGGTGGATGCACTATATgaacaccacg                                        >  2:653630/1‑92 (MQ=255)

                                                         tACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACgg                                       <  1:3322879/100‑1 (MQ=255)

                                                           cGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCa                                     <  1:1606942/100‑1 (MQ=255)

                                                           cGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCa                                     >  2:3013253/1‑100 (MQ=255)

                                                           cGTGCACGATTCGATCCGTGGCACGTTGCCGAAGCCGAAAAAGAAGGTATCGAATGCATCCCCGGAGCGACGGCGGATGCACTGTATGAAGAAAACGGc                                      >  2:662226/1‑99 (MQ=255)

                                                            gTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCag                                    >  2:3565388/1‑100 (MQ=255)

                                                            gTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCag                                    >  1:2657673/1‑100 (MQ=255)

                                                            gTGCACGATTCGAGCTGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCag                                    <  2:3432107/100‑1 (MQ=255)

                                                              gCACGATTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCagag                                  <  1:1327303/100‑1 (MQ=255)

                                                              gCACCACTCGATCCGTGCCCGGCTGCCGAGGCCGAAAAATCAGGTCCCGACTGCATCCCCGGAGCGACGGCGGCTGCACTGTATGAACAAAGCGGCagag                                  >  2:643630/1‑100 (MQ=255)

                                                               cACGATTCGATCCGTGGCGGGTTGCCGAAGGCAAAAAAGAAGGTGTCGAATGCATACCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCagag                                  <  2:2631374/99‑1 (MQ=255)

                                                                   aTTCGATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCtgt                             <  1:2007049/100‑1 (MQ=255)

                                                                    ttCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTCTCGACTGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCtgtg                            >  2:1350382/1‑100 (MQ=255)

                                                                      cgctCCGTGGCTGGTTGCCCAAGCCAAAAAAGGAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTgg                           <  2:2046842/96‑1 (MQ=255)

                                                                      cGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGc                          <  1:3386784/100‑1 (MQ=255)

                                                                       gATCCGTGGCTTGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCg                         <  2:3827901/100‑1 (MQ=255)

                                                                       gATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCg                         >  1:630885/1‑100 (MQ=255)

                                                                       gATCCGTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCg                         >  2:3722103/1‑100 (MQ=255)

                                                                           cGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTAt                     >  2:286344/1‑100 (MQ=255)

                                                                            gTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTAtt                    >  2:2553824/1‑100 (MQ=255)

                                                                            gTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTAtt                    <  2:704521/100‑1 (MQ=255)

                                                                            gTGGCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTAtt                    <  1:680382/100‑1 (MQ=255)

                                                                              ggCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTtg                  >  2:153475/1‑100 (MQ=255)

                                                                              ggCTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTtg                  <  2:981565/100‑1 (MQ=255)

                                                                                cTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTtgtg                >  2:2270940/1‑100 (MQ=255)

                                                                                cTGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTtgtg                <  1:2970856/100‑1 (MQ=255)

                                                                                 tGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTgtgg               <  1:2538082/100‑1 (MQ=255)

                                                                                 tGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTgtgg               <  1:3372381/100‑1 (MQ=255)

                                                                                 tGGTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTgtgg               <  2:620156/100‑1 (MQ=255)

                                                                                  ggTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTgtggt              <  1:1182483/100‑1 (MQ=255)

                                                                                  ggTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTgtgg               <  2:3909161/99‑1 (MQ=255)

                                                                                   gTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCTGAGCGACGGTGGATGCACTGTATGAAGAACCCGGCAGAGTCTGTGGCGTTATTTgtggtt             <  2:2597628/100‑2 (MQ=255)

                                                                                   gTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCGGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTgtggtg             <  2:2243365/100‑1 (MQ=255)

                                                                                   gTTGCCGAAGCCAAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTgtggtg             >  1:3834346/1‑100 (MQ=255)

                                                                                      gccgtagccaaaATAGAAGGTATCGAATCCAGCCCGGGAGCGATGGTGGATGCTCTGTATGAAGGAAACGCCTGAGTCTGTGGCGTTATTTgtgg               <  2:2755134/95‑1 (MQ=255)

                                                                                      gccgaagccgaaAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACg          >  2:328112/1‑100 (MQ=255)

                                                                                      gccgaagccgaaAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACg          <  1:448100/100‑1 (MQ=255)

                                                                                      gccgaagccaaaAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACg          >  1:3148411/1‑100 (MQ=255)

                                                                                      gccgaagccaaaAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACg          >  2:3181483/1‑100 (MQ=255)

                                                                                      gccgaagccaaaAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACg          <  2:3328043/100‑1 (MQ=255)

                                                                                       ccgaagccgaaAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGa         >  1:2800469/1‑100 (MQ=255)

                                                                                       ccgaagccgaaAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGa         >  1:1151634/1‑100 (MQ=255)

                                                                                             ccgaaAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGATATtct   >  2:2397568/1‑100 (MQ=255)

CCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGATATTCTC  >  NC_000913/2892898‑2893091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`