Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,964,868 T→C 53.4% K96K (AAA→AAG)  thyA ← thymidylate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,964,8680TC53.4% 54.2 / 225.1 180K96K (AAA→AAG) thyAthymidylate synthetase
Reads supporting (aligned to +/- strand):  ref base T (45/39);  new base C (46/50);  total (91/89)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 189 total aligned reads displayed.

AATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTG  >  NC_000913/2964769‑2964966
                                                                                                   |                                                                                                  
aataatGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGt                                                                                                    <  1:2960531/100‑1 (MQ=255)
 ataatGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGtt                                                                                                   <  1:2256484/100‑1 (MQ=255)
  taatGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGctt                                                                                                  <  2:2604195/100‑1 (MQ=255)
   aatGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTa                                                                                                 >  1:1177775/1‑100 (MQ=255)
    atGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTAc                                                                                                <  2:656995/100‑1 (MQ=255)
     tGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTAcc                                                                                               <  1:3033483/100‑1 (MQ=255)
     tGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTAcc                                                                                               >  2:538989/1‑100 (MQ=255)
     tGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTAcc                                                                                               >  1:3268608/1‑100 (MQ=255)
     tGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTAcc                                                                                               <  1:2281931/100‑1 (MQ=255)
     tGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATAGGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTAcc                                                                                               <  1:2024316/100‑1 (MQ=255)
        ggcgCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATa                                                                                            >  2:2686765/1‑100 (MQ=255)
        ggcgCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATa                                                                                            <  2:4073467/100‑1 (MQ=255)
        ggcgCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATa                                                                                            <  1:2669003/100‑1 (MQ=255)
         gcgcGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATac                                                                                           <  1:1238467/100‑1 (MQ=255)
          cgcgAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATTTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATaca                                                                                          >  2:254427/1‑100 (MQ=255)
           gcgAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATacac                                                                                         <  2:729618/100‑1 (MQ=255)
           gcgAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATacac                                                                                         <  1:856852/100‑1 (MQ=255)
            cgAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACt                                                                                        <  2:1307660/100‑1 (MQ=255)
             gAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTg                                                                                       >  2:3439584/1‑100 (MQ=255)
                  cGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCg                                                                                  >  1:180118/1‑100 (MQ=255)
                   gggTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGa                                                                                 >  2:3385321/1‑100 (MQ=255)
                    ggTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAg                                                                                <  2:464870/100‑1 (MQ=255)
                    ggTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAg                                                                                <  1:2926846/100‑1 (MQ=255)
                     gTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAgg                                                                               <  1:3570494/100‑1 (MQ=255)
                     gTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAgg                                                                               <  1:1409831/100‑1 (MQ=255)
                      tCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAggn                                                                              <  2:3826897/100‑2 (MQ=255)
                        gTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGTCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCg                                                                            >  1:3332611/1‑100 (MQ=255)
                        gTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCg                                                                            >  2:1858244/1‑100 (MQ=255)
                         tttttCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGc                                                                           <  2:3901864/100‑1 (MQ=255)
                         tttttCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGc                                                                           >  2:2423424/1‑100 (MQ=255)
                          ttttCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGcc                                                                          >  1:1019216/1‑100 (MQ=255)
                           tttCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCg                                                                         <  1:3493996/100‑1 (MQ=255)
                             tCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGtt                                                                       <  1:4049269/100‑1 (MQ=255)
                              cAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGttt                                                                      <  1:3657449/100‑1 (MQ=255)
                               aGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGtttt                                                                     >  1:1680562/1‑100 (MQ=255)
                               aGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGtttt                                                                     <  2:2736543/100‑1 (MQ=255)
                                gCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTc                                                                    >  2:3981788/1‑100 (MQ=255)
                                  tGGTTCAGTACCGTAGTGATCTGGTCAATATGACGCCCATCTGGCGCAGGCCCGGCGCGCCACTGTTCACCATACACTGGCCCGCGGCCGCCGTTTTc                                                                    >  2:1359659/1‑98 (MQ=255)
                                  tGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCAt                                                                  >  1:979317/1‑100 (MQ=255)
                                   ggTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATc                                                                 >  1:1480384/1‑100 (MQ=255)
                                    gTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCAATGTTGACCATACACTGGCCCGAGGTCGCCTTTTTCAt                                                                  >  2:3216311/1‑98 (MQ=255)
                                     ttaaGTACCGTAGTGATCTAGACAATATGACGACCTTCTGGCGTTGGCCAGGCGCGCTACTGCTTACCATGCACTGGCCCGAGGTCGCCGTTTTCATCgg                                                               <  2:3503888/97‑1 (MQ=255)
                                     ttCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCgg                                                               <  1:2355858/100‑1 (MQ=255)
                                      tCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGc                                                              >  1:486450/1‑100 (MQ=255)
                                        aGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGccc                                                            <  1:3807899/100‑1 (MQ=255)
                                        aGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGccc                                                            <  2:290880/100‑1 (MQ=255)
                                        aGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGccc                                                            >  1:699162/1‑100 (MQ=255)
                                         gTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCa                                                           <  1:3398854/100‑1 (MQ=255)
                                             cGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCg                                                       >  1:4078131/1‑100 (MQ=255)
                                              gTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGGCGCCGTTTTCATCGGCCCATTCGt                                                      >  2:1979151/1‑100 (MQ=255)
                                               tAGTGATCTGGTCAATATGGCGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTc                                                     <  2:1216487/100‑1 (MQ=255)
                                               tAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTc                                                     <  1:3619180/100‑1 (MQ=255)
                                                aGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTcc                                                    >  2:1487133/1‑100 (MQ=255)
                                                 gTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTccc                                                   <  1:3744678/100‑1 (MQ=255)
                                                 gTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTccc                                                   <  1:1349615/100‑1 (MQ=255)
                                                 gTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTccc                                                   <  2:2307587/100‑1 (MQ=255)
                                                   gATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAg                                                 >  1:2768218/1‑100 (MQ=255)
                                                   gATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAg                                                 >  1:2686920/1‑100 (MQ=255)
                                                   gATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAg                                                 >  2:797659/1‑100 (MQ=255)
                                                   gATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAg                                                 >  2:271466/1‑100 (MQ=255)
                                                      cTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCaagat                                              >  1:1044894/1‑96 (MQ=255)
                                                      cTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCCTTCTTCCCAg                                                 >  2:3939763/1‑97 (MQ=255)
                                                       tGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATgg                                             <  1:1394507/100‑1 (MQ=255)
                                                        ggTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGt                                            >  1:2243352/1‑100 (MQ=255)
                                                        ggTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGAGGGt                                            >  2:2818532/1‑100 (MQ=255)
                                                        ggTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGt                                            <  2:876150/100‑1 (MQ=255)
                                                         gTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTg                                           <  1:2316407/100‑1 (MQ=255)
                                                         gTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTg                                           <  1:1621408/100‑1 (MQ=255)
                                                         gTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTg                                           >  1:798863/1‑100 (MQ=255)
                                                          tCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGa                                          <  2:137137/100‑1 (MQ=255)
                                                           cAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGAc                                         >  1:1745114/1‑100 (MQ=255)
                                                           cAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGAc                                         <  2:2223473/100‑1 (MQ=255)
                                                             atatGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACAt                                       <  1:3482234/100‑1 (MQ=255)
                                                              tatGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACAtt                                      >  2:3100532/1‑100 (MQ=255)
                                                               atGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTg                                     <  2:3377684/100‑1 (MQ=255)
                                                               aggacgacCATCTGGCGTTGGCCAGGCGCGCCACTGTTTAGCATAGACTGGCCCGAGGTCGGCGTTTTCATCGGCCCATTCGTCCCAGATGGTGAc                                         <  2:249580/94‑1 (MQ=255)
                                                                   cgacCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGtttt                                 <  2:1653042/100‑1 (MQ=255)
                                                                     acCATCTGGCGTTGGCCAGGCGCGCCTCTGTTTACCATACCCTGGACCGAGGTCGCCGCCTTCAGCGGATCATTCGTCCCAGATGGTGACACTGTTTTCg                               >  2:1067276/1‑100 (MQ=255)
                                                                     acCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCGCGAGGTCGCCGTGTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCg                               <  2:1502247/100‑1 (MQ=255)
                                                                     acCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCg                               <  2:1131575/100‑1 (MQ=255)
                                                                     acCATCTGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCg                               <  1:950692/100‑1 (MQ=255)
                                                                     acCATCTGACGTTGGCCAGGCGCGCCACTGCTTACCATAAACTGGCCCGAGGTCGCCCTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCg                               <  2:1430909/100‑1 (MQ=255)
                                                                        aTCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCgtgt                            <  1:243704/100‑1 (MQ=255)
                                                                          ntGGCGTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAg                          >  1:3294441/2‑100 (MQ=255)
                                                                               gTTGGCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAg                     <  1:1551292/100‑1 (MQ=255)
                                                                                  ggCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCaa                  >  2:1575652/1‑100 (MQ=255)
                                                                                  ggCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCaa                  >  2:1843770/1‑100 (MQ=255)
                                                                                   gCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGc                    >  2:3578436/1‑97 (MQ=255)
                                                                                   gCCAGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAAt                 >  1:1151132/1‑100 (MQ=255)
                                                                                      atgcgcgcCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGtt              >  2:709563/3‑100 (MQ=255)
                                                                                      aGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGtt              <  1:1818183/100‑1 (MQ=255)
                                                                                      aGGCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGtt              <  1:3917227/100‑1 (MQ=255)
                                                                                       ggCGCGCCACTTCTTACCACACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGtt              <  1:2674040/99‑1 (MQ=255)
                                                                                       ggCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTa             >  1:60317/1‑100 (MQ=255)
                                                                                       ggCGCGCCACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCACAGATGTTGACATTGTTTTCGTGTAGATAAGCAATGTTa             >  2:440618/1‑100 (MQ=255)
                                                                                         cgcgcCACTGTTTACCATACACTGGCCCGAGGTGGCCGTTTTCATCGGCCCATTCGTGCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAgt           >  2:318805/1‑100 (MQ=255)
                                                                                             ccACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCg       >  1:2807247/1‑100 (MQ=255)
                                                                                              cACTGCTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGc      <  2:4000512/100‑1 (MQ=255)
                                                                                                cTGCTTACCATACACTGGCCCGGGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGcc     <  2:169347/99‑1 (MQ=255)
                                                                                                  gTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTg  >  2:3025645/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
AATAATGCGGCGCGAATCCGGGTCGTTTTTCAGCTGGTTCAGTACCGTAGTGATCTGGTCAATATGACGACCATCTGGCGTTGGCCAGGCGCGCCACTGTTTACCATACACTGGCCCGAGGTCGCCGTTTTCATCGGCCCATTCGTCCCAGATGGTGACATTGTTTTCGTGTAGATAAGCAATGTTAGTGTCGCCCTG  >  NC_000913/2964769‑2964966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑