Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,998,129 C→T 51.2% pseudogene (700/1137 nt) ygeQ ← pseudogene, glycosyl hydrolase family 15, part of T3SS PAI ETT2 remnant

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,998,1290CT51.2% ‑2.3 / 266.2 191pseudogene (700/1137 nt)ygeQpseudogene, glycosyl hydrolase family 15, part of T3SS PAI ETT2 remnant
Reads supporting (aligned to +/- strand):  ref base C (41/52);  new base T (43/54);  total (84/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

Only 100 of 195 total aligned reads displayed.

ATGCTCAGGAATCTTCCATAACATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTCCACAGCTGCGGCTTGCCTTC  >  NC_000913/2998030‑2998225
                                                                                                   |                                                                                                
aTGCTCAGGAATCTTCCATAACATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGc                                                                                                  >  2:3878969/1‑100 (MQ=255)
aTGCTCAGGAATCTTCCATAACATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGc                                                                                                  <  1:3878994/100‑1 (MQ=255)
  gCTCAGGAATCTTCCATAACATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAgtgt                                                                                                <  2:3815125/100‑1 (MQ=255)
   cTCAGGAATCTTCCATAACATAGAAATTGTCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGtt                                                                                               <  2:771931/100‑1 (MQ=255)
   cTCAGGAATCTTCCATAACATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTTCCTATCTTTAgtgt                                                                                                >  2:3567043/1‑99 (MQ=255)
   cTCAGGAATCTTCCATAACATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGtt                                                                                               >  1:2096282/1‑100 (MQ=255)
   cTCAGGAATCTTCCATAACATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGtt                                                                                               <  1:1352744/100‑1 (MQ=255)
   cTCAGGAATCTTCCATAACATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGtt                                                                                               >  2:3460086/1‑100 (MQ=255)
    tCAGGAATCTTCCATAACATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTa                                                                                              <  1:3661286/100‑1 (MQ=255)
    tCAGGAATCTTCCATAACATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTa                                                                                              >  1:2306028/1‑100 (MQ=255)
        gAATCTTCCATAACATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAg                                                                                          <  2:1917723/100‑1 (MQ=255)
           tCTTCCATAACATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTAc                                                                                       <  1:1170581/100‑1 (MQ=255)
             ttCCATAACATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCACAACCGACTTTAATCGGTCACATTTTTGCCAATCTTCAGCGTTAATAGTACCa                                                                                     >  2:584771/1‑100 (MQ=255)
                cATAACATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAgtg                                                                                  <  1:1879366/100‑1 (MQ=255)
                 aTAACATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAgtgt                                                                                 >  2:1435306/1‑100 (MQ=255)
                   aaCATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTg                                                                               >  1:2433734/1‑100 (MQ=255)
                    aCATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGa                                                                              >  2:1481750/1‑100 (MQ=255)
                     cATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGAt                                                                             <  1:1512788/100‑1 (MQ=255)
                       tAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTg                                                                           >  1:191326/1‑100 (MQ=255)
                       tAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTg                                                                           <  2:110378/100‑1 (MQ=255)
                         gAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCt                                                                         >  1:1866685/1‑100 (MQ=255)
                         gAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCt                                                                         >  1:3356193/1‑100 (MQ=255)
                           aaTTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTg                                                                       <  1:662083/100‑1 (MQ=255)
                           aaTTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTg                                                                       <  1:2538933/100‑1 (MQ=255)
                           aaTTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTg                                                                       <  2:4082478/100‑1 (MQ=255)
                           aaTTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTg                                                                       <  2:1574009/100‑1 (MQ=255)
                           aaTTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTCTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTg                                                                       <  1:1018386/100‑1 (MQ=255)
                           aaTTGGCTTGATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTg                                                                       <  1:4117827/100‑1 (MQ=255)
                           aaTGGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTg                                                                       <  2:685750/100‑1 (MQ=255)
                             ttGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGta                                                                     <  1:1639730/100‑1 (MQ=255)
                               ggCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGtata                                                                   <  2:2513045/100‑1 (MQ=255)
                               ggCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGtata                                                                   >  1:3604239/1‑100 (MQ=255)
                                gCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATaa                                                                  >  1:2123050/1‑100 (MQ=255)
                                 cgttATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAg                                                                 <  1:3709893/98‑1 (MQ=255)
                                 cTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAg                                                                 >  1:1187898/1‑100 (MQ=255)
                                  tttATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAgg                                                                >  1:3421476/1‑100 (MQ=255)
                                  tttATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAgg                                                                >  1:3476289/1‑100 (MQ=255)
                                  cttATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAgg                                                                <  1:79873/99‑1 (MQ=255)
                                    tATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGga                                                               >  2:79873/1‑99 (MQ=255)
                                    tATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGgag                                                              >  1:2687342/1‑100 (MQ=255)
                                      tCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGAt                                                            <  1:54889/100‑1 (MQ=255)
                                        cAGATACGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTCTTGCTTGCTTGTCTAAGGCGATCa                                                          >  2:2027695/1‑100 (MQ=255)
                                        cAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCa                                                          >  1:295596/1‑100 (MQ=255)
                                          gATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAg                                                        <  2:3626301/100‑1 (MQ=255)
                                           aTAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAgg                                                       <  2:2228532/100‑1 (MQ=255)
                                           aTAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAgg                                                       <  1:424002/100‑1 (MQ=255)
                                           aTAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAgg                                                       <  2:2100418/100‑1 (MQ=255)
                                             aaGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTTCCAATCTTCATCGTTAATAGTACCAGTGTTGATTGCTTCTATAAGGAGATCAAGGTa                                                     >  2:1204160/1‑100 (MQ=255)
                                             aaGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTa                                                     <  1:3325699/100‑1 (MQ=255)
                                              aGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTaa                                                    <  1:3195821/100‑1 (MQ=255)
                                               gCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTaaa                                                   <  2:1841636/100‑1 (MQ=255)
                                                 aaTCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGa                                                 <  1:3847223/100‑1 (MQ=255)
                                                  aTCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGAc                                                >  1:3168128/1‑100 (MQ=255)
                                                   tCAGCAGCGCAACCGCCTTTAATCGGTAACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGAGTTGATTGCTTGTATAAGGAAATTAAGGTAAAGAcc                                               >  2:488009/1‑100 (MQ=255)
                                                   tCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGAcc                                               <  1:2573775/100‑1 (MQ=255)
                                                   tCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGAcc                                               <  1:1967656/100‑1 (MQ=255)
                                                    cagcagCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCt                                              >  2:2379505/1‑100 (MQ=255)
                                                    cagcagCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCt                                              <  1:1301076/100‑1 (MQ=255)
                                                     agcagcGCAACCGACTTTAATCGGTCACCTTTTTTTTTTTTTTTTTTGTTAATAGTACCATTGTTTATTTCTTCTATAAGGAGATCAAGGTAAAGActt                                              >  2:3747601/1‑97 (MQ=255)
                                                      gcagcGTAACCGACTTGAAGTGCTCAGGCTTGTGCAAAACTTCAGCGTTAATAGTAGCAGTGTTGATTGCTTGTATAAGGGGATCAAgg                                                       <  2:3582847/89‑1 (MQ=255)
                                                       cagcGCAACCTACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATCGCTTGTATAAGGAGATCAAGGTAAAGACCTaaa                                           <  2:2722508/100‑1 (MQ=255)
                                                         gcgcAACCGACTTTAATCGGTCACCTTTTTTTTGATCTTCAGTGTTAATAGTACCAGTGTTGATTGTTTTTATATTGTGTTCAAGGTAAAGACCTa                                             >  2:1014783/1‑96 (MQ=255)
                                                         gcgcAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGc                                         <  1:1041688/100‑1 (MQ=255)
                                                          cgcAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGca                                        <  2:3231556/100‑1 (MQ=255)
                                                           gcAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGcat                                       >  2:1526883/1‑100 (MQ=255)
                                                            cAACCGACTTTAATCGGTCACCTTTTTTTTTTTTTTCATTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGCGATCAAGGTAAAGACCTAAAGcatc                                      >  2:10284/1‑100 (MQ=255)
                                                             agccGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAg                                          <  2:2632877/93‑1 (MQ=255)
                                                              aCCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGcatcat                                    >  2:3059450/1‑100 (MQ=255)
                                                               ccGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCAtt                                   >  1:959131/1‑100 (MQ=255)
                                                                  aCTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTg                                <  2:3854235/100‑1 (MQ=255)
                                                                  aCTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTg                                <  1:1556630/100‑1 (MQ=255)
                                                                   cTTTAATCGGTCACGTTTTTGCAAATCTTCAGGGTGAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTg                                <  2:1935949/99‑1 (MQ=255)
                                                                   cTTTAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGt                               >  1:739920/1‑100 (MQ=255)
                                                                     ttAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGttt                             >  1:2941301/1‑100 (MQ=255)
                                                                     ttAATCGGTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGtt                              <  2:914738/99‑1 (MQ=255)
                                                                        aTCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATg                          <  1:3179285/100‑1 (MQ=255)
                                                                         tCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATgg                         <  1:226578/100‑1 (MQ=255)
                                                                           ggTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGtt                       <  2:3509975/100‑1 (MQ=255)
                                                                            gTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTc                      >  2:526738/1‑100 (MQ=255)
                                                                            gTCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTc                      <  2:3623637/100‑1 (MQ=255)
                                                                             tCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTcc                     <  2:4137849/100‑1 (MQ=255)
                                                                             tCACCTTTTTGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTcc                     <  2:2432571/100‑1 (MQ=255)
                                                                              cACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTCca                    <  2:3200426/100‑1 (MQ=255)
                                                                              cACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTCca                    >  1:2465204/1‑100 (MQ=255)
                                                                                 cTTTTTGCCAATCTTAAGCGTTAATAGTACAAGTGTTGGTTGATTGTATACGGAGATCAAAGTAAAGAGCTAAAGGATCATTTTGTTTATGGttgccc                   >  2:3698796/1‑94 (MQ=255)
                                                                                  tttttGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAATACCTAAAGCATCATTTTGTTTATGGTTcc                     <  2:2815961/95‑1 (MQ=255)
                                                                                     ttGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTCCACAGCTGc             <  2:1236487/100‑1 (MQ=255)
                                                                                      tGCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATTATTTTGTTTATGGTTCCACAGCTGCg            <  2:3734918/100‑1 (MQ=255)
                                                                                       gCCAATCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTCCACAGCTGCgg           <  1:1793447/100‑1 (MQ=255)
                                                                                       gCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTCCACAGCTGCgg           >  2:662557/1‑100 (MQ=255)
                                                                                        ctaaTCTTCAGCGTTAATAGTCCCATTGTTGATTGCTTGTATAAGGTGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTCCACAGCTGCGGc          <  1:822012/98‑1 (MQ=255)
                                                                                        ccAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTCCACAGCTGCGGc          >  2:3950445/1‑100 (MQ=255)
                                                                                        ccAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTCCACAGCTGCGGc          >  1:2853704/1‑100 (MQ=255)
                                                                                        ccAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTCCACAGCTGCGGc          <  1:2452273/100‑1 (MQ=255)
                                                                                           aTCTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTCCACAGCTGCGGCTTg       <  2:1256701/100‑1 (MQ=255)
                                                                                             cTTCAGTGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTCCACAGCTGCGGCTTGcc     <  1:603724/100‑1 (MQ=255)
                                                                                              ttCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTCCACAGCTGCGGCTTGCct    >  1:1400194/1‑100 (MQ=255)
                                                                                               tCAGCGTTAATAGTACCAGTGTTGATTTCTTGTATATGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTACACAGCTGCGGCTTGCctt   <  2:3387196/100‑1 (MQ=255)
                                                                                                cAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGATATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTCCACAGCTGCGGCTTGCcttc  <  2:958967/100‑1 (MQ=255)
                                                                                                cAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTCCACAGCTGCGGCTTGCcttc  >  2:2990773/1‑100 (MQ=255)
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ATGCTCAGGAATCTTCCATAACATAGAAATTGGCTTTATCCAGATAAGCAATCAGCAGCGCAACCGACTTTAATCGGTCACCTTTTTGCCAATCTTCAGCGTTAATAGTACCAGTGTTGATTGCTTGTATAAGGAGATCAAGGTAAAGACCTAAAGCATCATTTTGTTTATGGTTCCACAGCTGCGGCTTGCCTTC  >  NC_000913/2998030‑2998225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: