BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	344,961	T→C	56.0%	N262N (AAT→AAC)	yahL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	344,961	0	T	C	56.0%	65.3 / 202.1	164	N262N (AAT→AAC)	yahL	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (34/38); new base C (47/45); total (81/83)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01

Only 100 of 164 total aligned reads displayed.

TCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCA  >  NC_000913/344863‑345056

 ctGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATaa                                                                                               >  1:239235/1‑100 (MQ=255)

 ctGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACaa                                                                                               >  2:3670468/1‑100 (MQ=255)

 ctGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACaa                                                                                               >  2:807923/1‑100 (MQ=255)

   gTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGc                                                                                             <  2:2754891/100‑1 (MQ=255)

   gTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGc                                                                                             >  2:3947511/1‑100 (MQ=255)

    ttCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCa                                                                                            <  1:668556/100‑1 (MQ=255)

        ggACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGtt                                                                                        >  2:3557180/1‑100 (MQ=255)

        ggACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGtt                                                                                        >  2:2195615/1‑100 (MQ=255)

          aCGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTaa                                                                                      <  1:1689761/100‑1 (MQ=255)

           cGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTaaa                                                                                     >  2:3768217/1‑100 (MQ=255)

             tAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAAt                                                                                   >  2:1543844/1‑100 (MQ=255)

               aaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATcc                                                                                 <  1:258263/100‑1 (MQ=255)

               aaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATcc                                                                                 >  1:2696168/1‑100 (MQ=255)

                 aaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGc                                                                               <  1:2178644/100‑1 (MQ=255)

                  aCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCt                                                                              <  2:3160938/100‑1 (MQ=255)

                  aCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCt                                                                              <  2:887624/100‑1 (MQ=255)

                  aCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCt                                                                              <  2:3678224/100‑1 (MQ=255)

                  aCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCt                                                                              <  1:976654/100‑1 (MQ=255)

                  aCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCt                                                                              <  2:1270642/100‑1 (MQ=255)

                   cATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTa                                                                             <  1:3338164/100‑1 (MQ=255)

                    aTACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTac                                                                            <  2:4022979/100‑1 (MQ=255)

                    aTACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTac                                                                            >  1:288301/1‑100 (MQ=255)

                      aCAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTacac                                                                          <  1:1887482/100‑1 (MQ=255)

                       cAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACAct                                                                         >  1:3645546/1‑100 (MQ=255)

                       cAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACAct                                                                         >  1:3646998/1‑100 (MQ=255)

                       cAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACAct                                                                         >  2:3899457/1‑100 (MQ=255)

                        agagATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACActc                                                                        >  1:3525818/1‑100 (MQ=255)

                         gagaTGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACActct                                                                       >  1:1093989/1‑100 (MQ=255)

                           gatgatGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCttt                                                                     <  2:161437/100‑1 (MQ=255)

                           gatgatGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCttt                                                                     >  1:163117/1‑100 (MQ=255)

                             tgatgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAg                                                                   <  1:2272172/100‑1 (MQ=255)

                                tgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTc                                                                <  2:3492134/100‑1 (MQ=255)

                                tgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTc                                                                <  1:113511/100‑1 (MQ=255)

                                tgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTc                                                                >  2:1265686/1‑100 (MQ=255)

                                  cgcgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAg                                                              >  1:1866272/1‑100 (MQ=255)

                                  cgcgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAg                                                              >  2:1038909/1‑100 (MQ=255)

                                       ttAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAgg                                                         <  1:2938273/100‑1 (MQ=255)

                                         aaaTGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGt                                                       >  1:2072881/1‑100 (MQ=255)

                                            tgaatgGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTcc                                                    <  1:691817/100‑1 (MQ=255)

                                               atgGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCAcc                                                 <  1:1271128/100‑1 (MQ=255)

                                               atctatatCTAATATATTGCAGGAATTAAGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCAcc                                                 <  2:2352777/96‑1 (MQ=255)

                                                tgGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCt                                                >  2:2983814/1‑100 (MQ=255)

                                                 ggATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTg                                               >  1:3952807/1‑100 (MQ=255)

                                                 ggATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTg                                               <  1:1624468/100‑1 (MQ=255)

                                                    tatCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCaa                                            >  2:2660972/1‑100 (MQ=255)

                                                    tatCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCa                                             >  1:2551192/1‑99 (MQ=255)

                                                      tCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAgt                                          >  2:2833216/1‑100 (MQ=255)

                                                      tCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAgt                                          <  1:592570/100‑1 (MQ=255)

                                                       cTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAgtt                                         >  1:3237947/1‑100 (MQ=255)

                                                       cTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAgtt                                         >  2:548435/1‑100 (MQ=255)

                                                       cTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAgtt                                         >  1:540986/1‑100 (MQ=255)

                                                        tAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACCCGCTTTAGTTCAGAAAGGGTTCCACCTGCAAgttg                                        <  1:1309848/100‑1 (MQ=255)

                                                        tAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAgttg                                        <  2:3952781/100‑1 (MQ=255)

                                                          aTAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAgttgtt                                      <  1:2958067/100‑1 (MQ=255)

                                                             gagTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                   <  1:474603/100‑1 (MQ=255)

                                                             gagTGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                   >  1:1801944/1‑100 (MQ=255)

                                                               gTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACTCTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTcc                                 <  1:357100/100‑1 (MQ=255)

                                                               gTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTcc                                 <  2:3588615/100‑1 (MQ=255)

                                                                tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCa                                >  1:3775422/1‑100 (MQ=255)

                                                                tGCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCa                                <  2:912629/100‑1 (MQ=255)

                                                                 tcAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAc                               <  2:697168/99‑1 (MQ=255)

                                                                 gCAGGAATTGGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAc                               >  2:408367/1‑100 (MQ=255)

                                                                 gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAc                               >  1:3505713/1‑100 (MQ=255)

                                                                 gCAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAc                               >  2:1501320/1‑100 (MQ=255)

                                                                  cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAcc                              <  2:3881342/100‑1 (MQ=255)

                                                                  cAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCTACCTGCAAGTTTTTATTCCAc                               >  2:464985/1‑99 (MQ=255)

                                                                  cAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAcc                              <  2:3371687/100‑1 (MQ=255)

                                                                  cAGGAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCAcc                              <  2:2696152/100‑1 (MQ=255)

                                                                     gAATTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTga                           >  2:885680/1‑100 (MQ=255)

                                                                      aaTTAGGTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgag                          >  1:3877332/1‑100 (MQ=255)

                                                                       aTTAGATGACAGATATTACCCTGTAAATAAGCACGTTAACAACCGCTACTCCCTATAGCTCAGAAAGGGATCCACCGGCAAGTCGTTATTCCACCTgcg                          <  2:2273782/99‑3 (MQ=255)

                                                                         tAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGt                       <  2:714018/100‑1 (MQ=255)

                                                                           ggTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGa                     >  1:2245556/1‑100 (MQ=255)

                                                                           ggTGACAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGa                     >  1:800873/1‑100 (MQ=255)

                                                                            atctcAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGaa                    <  1:1991732/96‑1 (MQ=255)

                                                                             tGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAAt                   <  1:2833232/100‑1 (MQ=255)

                                                                              gACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATc                  >  1:1454861/1‑100 (MQ=255)

                                                                              gACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATc                  <  2:1905529/100‑1 (MQ=255)

                                                                               aCAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCg                 >  2:1235378/1‑100 (MQ=255)

                                                                                cAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGc                <  1:519999/100‑1 (MQ=255)

                                                                                cAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGc                >  1:3456070/1‑100 (MQ=255)

                                                                                cAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGaaagat                >  2:1991719/1‑96 (MQ=255)

                                                                                cAGATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGc                >  2:3315758/1‑100 (MQ=255)

                                                                                  gATATTATCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCaa              <  1:3090839/100‑1 (MQ=255)

                                                                                   atatTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAAt             <  2:2020506/100‑1 (MQ=255)

                                                                                       tatCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAg         >  1:1751429/1‑100 (MQ=255)

                                                                                         tCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGt       <  1:662796/100‑1 (MQ=255)

                                                                                         tCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGt       <  2:1709462/100‑1 (MQ=255)

                                                                                         tCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGt       <  1:2289576/100‑1 (MQ=255)

                                                                                         tCCTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGt       <  2:3537277/100‑1 (MQ=255)

                                                                                          ccTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTa      >  2:3107572/1‑100 (MQ=255)

                                                                                          ccTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTa      >  1:1326901/1‑100 (MQ=255)

                                                                                          ccTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTa      >  1:1183796/1‑100 (MQ=255)

                                                                                           cTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTaa     >  1:3990566/1‑100 (MQ=255)

                                                                                           cTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTaa     >  1:807159/1‑100 (MQ=255)

                                                                                           cTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTaa     >  2:1803477/1‑100 (MQ=255)

                                                                                           cTGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCGGAAAGGGTTCCACCTGCAAATTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTaa     >  2:2248123/1‑100 (MQ=255)

                                                                                            tGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAAt    <  1:3768241/100‑1 (MQ=255)

                                                                                            tGTAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAAt    <  2:1326892/100‑1 (MQ=255)

                                                                                              tAAACAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCa  >  1:1052374/1‑100 (MQ=255)

TCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCA  >  NC_000913/344863‑345056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`