Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,041,028 G→A 20.8% R15C (CGT→TGT)  sdhE ← flavinator of succinate dehydrogenase; antitoxin of CptAB toxin‑antitoxin pair

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,041,0280GA20.8% 363.7 / 79.1 173R15C (CGT→TGT) sdhEflavinator of succinate dehydrogenase; antitoxin of CptAB toxin‑antitoxin pair
Reads supporting (aligned to +/- strand):  ref base G (60/76);  new base A (18/18);  total (78/95)
Fisher's exact test for biased strand distribution p-value = 5.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

CCAGCAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCC  >  NC_000913/3040929‑3041125
                                                                                                   |                                                                                                 
ccAGCAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAACACGGCATGATTGAAATATCGAGTTCGCGCATACCAcg                                                                                                   >  1:713390/1‑100 (MQ=255)
  agcagACTAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcggc                                                                                                 >  1:1117868/1‑100 (MQ=255)
    cagACGAATAAAGATGCGTTTTTTTTTTTTTTTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGTGCATACCAcagcgg                                                                                               >  2:4132532/1‑100 (MQ=255)
    cagACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcggcgg                                                                                               >  1:1129572/1‑100 (MQ=255)
    cagACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCGGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcggcgg                                                                                               <  1:2336670/100‑1 (MQ=255)
     agACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACggcggc                                                                                              <  2:4040500/100‑1 (MQ=255)
      gACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCa                                                                                             <  2:3142114/100‑1 (MQ=255)
         gAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCCTGATTGCAATATCGAGTTCGCGCATACCCCGGCGGCATg                                                                                           >  2:4070534/1‑99 (MQ=255)
         gAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGc                                                                                          >  1:3817471/1‑100 (MQ=255)
           aTAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGccc                                                                                        <  1:3065311/100‑1 (MQ=255)
            tAAAGATGCGTTTTTCGTCACCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCa                                                                                       <  2:3099877/100‑1 (MQ=255)
              aaGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCaat                                                                                     >  2:3787137/1‑100 (MQ=255)
               ataTGCGTTTTTCGTCATCGCTTAAGAAGACATAAACATATAAGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCaa                                                                                      <  2:3567600/96‑1 (MQ=255)
                  tGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCaatgaat                                                                                 >  1:1758187/1‑100 (MQ=255)
                  tGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCaatgaat                                                                                 >  1:2195635/1‑100 (MQ=255)
                    cGTTTTTCTTTATCGCTTAAACTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGATCGCGCATACCACGACGGCATGCCCaatgaat                                                                                 >  2:1591678/1‑98 (MQ=255)
                     gTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCg                                                                              >  2:802365/1‑100 (MQ=255)
                       ttttCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCggg                                                                            >  2:2425785/1‑100 (MQ=255)
                         ttCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCt                                                                          <  2:2171274/100‑1 (MQ=255)
                           ngTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGATCGCGCATACCACGGAGGCATGCCCAATGAATGCGGGCttt                                                                        >  1:3845704/2‑100 (MQ=255)
                           cGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCttt                                                                        >  1:755941/1‑100 (MQ=255)
                           cGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCttt                                                                        <  2:1118945/100‑1 (MQ=255)
                            gTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTttg                                                                       >  1:627098/1‑100 (MQ=255)
                            gTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTttg                                                                       <  1:3032129/100‑1 (MQ=255)
                            gTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTttg                                                                       >  1:1368267/1‑100 (MQ=255)
                              cATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTttgtt                                                                     >  1:3782007/1‑100 (MQ=255)
                              cATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTtcgtt                                                                     >  1:786375/1‑100 (MQ=255)
                               aTCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTttgttg                                                                    <  2:529379/100‑1 (MQ=255)
                               aTCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTttgttg                                                                    <  1:3001096/100‑1 (MQ=255)
                                tCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGCTTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTtgttgt                                                                   <  2:2906789/100‑1 (MQ=255)
                                tCGCTTAAGCTGTCGTACTAATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTtgttgt                                                                   <  2:44476/100‑1 (MQ=255)
                                 cGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTgttgtt                                                                  >  2:3210738/1‑100 (MQ=255)
                                 cGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTgttgtt                                                                  <  1:2243502/100‑1 (MQ=255)
                                  gCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAGTATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTa                                                                 >  2:208615/1‑100 (MQ=255)
                                   cTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTaa                                                                <  1:1561551/100‑1 (MQ=255)
                                   cTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTaa                                                                <  1:1943226/100‑1 (MQ=255)
                                   cTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAAAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTaa                                                                >  1:3942358/1‑100 (MQ=255)
                                    ttAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAAt                                                               >  1:3270163/1‑100 (MQ=255)
                                     tAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATg                                                              >  2:1295463/1‑100 (MQ=255)
                                     tAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATg                                                              >  2:2731115/1‑100 (MQ=255)
                                      aaGCTGTCGTACTCATGTTGGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAGAGCGGCATGCCAAATGAATGCGGGCTTTGTTGTTAAtct                                                             >  2:1541172/1‑98 (MQ=255)
                                       ctcttccgatcTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTc                                                            <  1:2499575/90‑1 (MQ=255)
                                       aGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTc                                                            <  1:3948421/100‑1 (MQ=255)
                                         cTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCa                                                          <  1:2089274/100‑1 (MQ=255)
                                            tCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtct                                                       <  1:467591/100‑1 (MQ=255)
                                            tCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtct                                                       <  1:372094/100‑1 (MQ=255)
                                            tCGTAATAAAGTTCAAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtct                                                       <  2:1488048/100‑1 (MQ=255)
                                              ntACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtcttc                                                     >  2:3986563/2‑100 (MQ=255)
                                                 cTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCttcttc                                                  >  2:3854232/1‑100 (MQ=255)
                                                 cTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCttcttc                                                  >  2:2125350/1‑100 (MQ=255)
                                                  tCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTcc                                                 <  1:1910942/100‑1 (MQ=255)
                                                  tCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTcc                                                 <  1:3363570/100‑1 (MQ=255)
                                                  tCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTcc                                                 <  1:2776313/100‑1 (MQ=255)
                                                  tCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTcc                                                 <  2:1365592/100‑1 (MQ=255)
                                                  tCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTcc                                                 <  1:2475039/100‑1 (MQ=255)
                                                   cATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTcagatcggaa                                                   >  2:2499561/1‑88 (MQ=255)
                                                    aTGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTg                                               >  1:2032814/1‑100 (MQ=255)
                                                      gTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtc                                             >  1:913976/1‑100 (MQ=255)
                                                      gTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtc                                             >  2:2611018/1‑100 (MQ=255)
                                                       ttCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtct                                            >  1:314640/1‑100 (MQ=255)
                                                       ttCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtct                                            <  2:2383842/100‑1 (MQ=255)
                                                        tCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGACCATCTTCTTCCTGtctc                                           >  2:3541792/1‑100 (MQ=255)
                                                          gAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCAc                                         >  1:2456973/1‑100 (MQ=255)
                                                          gAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCAc                                         >  2:2073339/1‑100 (MQ=255)
                                                           aaaatCGGCATGATTGGAATATCGAGTTCGCGTATACCACAGCGGCATGCCCAATGAATGCGCGCTTTGTTGTTAATGTCCATCTTCTTCCTGtctct                                          <  2:164661/98‑2 (MQ=255)
                                                           aaaaaCGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACg                                        <  1:2673210/100‑1 (MQ=255)
                                                             aaaCGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGaa                                      <  2:3816309/100‑1 (MQ=255)
                                                             aaaCGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATTAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGaa                                      <  2:2292214/100‑1 (MQ=255)
                                                               aCGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGaaaa                                    <  1:1089019/100‑1 (MQ=255)
                                                                 ggCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATc                                  <  1:1575768/100‑1 (MQ=255)
                                                                 ggCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATc                                  >  2:2695764/1‑100 (MQ=255)
                                                                  tcATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATcc                                 <  2:3522684/99‑1 (MQ=255)
                                                                   cATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCa                                >  1:3522706/1‑100 (MQ=255)
                                                                    aTGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAg                               <  2:3911392/100‑1 (MQ=255)
                                                                     tGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGt                              <  1:1268041/100‑1 (MQ=255)
                                                                     tGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGt                              <  1:983993/100‑1 (MQ=255)
                                                                       aTTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTAc                            >  1:756499/1‑100 (MQ=255)
                                                                          gAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCg                         <  1:527998/100‑1 (MQ=255)
                                                                          gAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCg                         <  2:3703844/100‑1 (MQ=255)
                                                                            aaTATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGc                       <  2:3813832/100‑1 (MQ=255)
                                                                            aaTATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTATTCCTGTCTCTAGAAAATCCAGTAccc                          >  2:298547/1‑97 (MQ=255)
                                                                              tatCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAgagatcggaag                     >  1:168125/1‑90 (MQ=255)
                                                                               atCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTAt                    >  1:1491744/1‑100 (MQ=255)
                                                                               atCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTAt                    <  1:2550839/100‑1 (MQ=255)
                                                                                 cGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTAtt                   >  2:179499/1‑99 (MQ=255)
                                                                                   aGTTCGCGCATACCACGGCGGCATGCCCAATGATTGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTGTCACGAAAATCCAGTACCCGGCTATTGTa                >  2:2478578/1‑100 (MQ=255)
                                                                                   aGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTa                <  2:957958/100‑1 (MQ=255)
                                                                                      ttccgatctccACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACg             <  1:1447338/91‑1 (MQ=255)
                                                                                      tCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACg             >  1:3176335/1‑100 (MQ=255)
                                                                                      tCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACg             >  1:1439927/1‑100 (MQ=255)
                                                                                       cgcgCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTAGCCGGCTATTGTAACgt            >  2:3020438/1‑100 (MQ=255)
                                                                                       cgcgCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACgt            >  2:3654015/1‑100 (MQ=255)
                                                                                         cgcATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACgtgt          <  1:1638373/100‑1 (MQ=255)
                                                                                          gcATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGtt         >  1:1674931/1‑100 (MQ=255)
                                                                                            aTACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGtttt       >  1:3329545/1‑100 (MQ=255)
                                                                                              aCCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCACTACCAGGCTATTGTAACGTGTTTTTc     >  1:1788260/1‑100 (MQ=255)
                                                                                               ccACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACgagatcggaa    >  2:1447328/1‑91 (MQ=255)
                                                                                               ccACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCg    >  2:168434/1‑100 (MQ=255)
                                                                                               ccACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCg    <  2:1117861/100‑1 (MQ=255)
                                                                                               caacgacgGCATGCCCAACGAATGCGGCCTTTGTTGTCAATGTCCACCTTCTTCCGGTCTCACGAAAATCCAGTACCCGGCTATAGTAACGTGttgtccg    <  2:2631443/98‑6 (MQ=255)
                                                                                                   |                                                                                                 
CCAGCAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCC  >  NC_000913/3040929‑3041125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: