Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,057,140 A→G 17.1% intergenic (‑191/+38) ibsC ← / ← serA toxic membrane protein/D‑3‑phosphoglycerate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,057,1400AG17.1% 434.5 / 83.4 206intergenic (‑191/+38)ibsC/serAtoxic membrane protein/D‑3‑phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (92/78);  new base G (18/17);  total (110/96)
Fisher's exact test for biased strand distribution p-value = 8.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.10e-01

Only 100 of 213 total aligned reads displayed.

CAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTT  >  NC_000913/3057041‑3057238
                                                                                                    |                                                                                                  
  aCGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGt                                                                                                  <  1:3171830/100‑1 (MQ=255)
   cGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTc                                                                                                 <  1:2421399/100‑1 (MQ=255)
    gCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGCGAAGGGCTAAAAAAACGGGCAAGTCa                                                                                                >  2:586472/1‑100 (MQ=255)
     cATTGATCTGACATTGATTTGTTCTCTGGAGCAGACTCGCAAAGGAGAATGCGTAACGCGGCAGCGGTGTGGAGAAGAGATAAAAAAACGGGGAAGTAAg                                                                                               <  2:4059497/100‑1 (MQ=255)
      aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCact                                                                                              <  2:2844732/100‑3 (MQ=255)
         gATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGAc                                                                                           <  1:3886925/100‑1 (MQ=255)
          aTCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGGATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAACAAACGGGCAAGACAGTGaac                                                                                          >  2:185104/1‑98 (MQ=255)
          aTCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGAcc                                                                                          >  2:1701045/1‑100 (MQ=255)
            cTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGAct                                                                                          <  2:1532490/98‑2 (MQ=255)
             tGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGc                                                                                       >  1:2094177/1‑100 (MQ=255)
               aCTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc                                                                                     <  2:2233206/100‑1 (MQ=255)
               aCTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc                                                                                     <  1:1701057/100‑1 (MQ=255)
                cTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCg                                                                                    <  2:1158024/100‑1 (MQ=255)
                cTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCg                                                                                    >  1:1587049/1‑100 (MQ=255)
                cTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTCGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCg                                                                                    >  2:3913845/1‑100 (MQ=255)
                cTTTGATTTATTTTCTGAAGCAGCCTCGCACAGTAGAATGCGCAACGCGGCACCGGCGCGGAGAAGGGATAAAAAAACGAGAAAGTAAGTTAcc                                                                                          >  2:3289759/1‑94 (MQ=255)
                 tttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGt                                                                                   <  1:3336131/100‑1 (MQ=255)
                 tttGATTTAGTTTCTGGAGCAGACCCGAAAAGTATAATGCGCAACGCTACAACGGTGTAGAGAAAGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGt                                                                                   <  1:1765841/100‑1 (MQ=25)
                    gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGtttt                                                                                <  1:1517240/100‑3 (MQ=38)
                    gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGtttt                                                                                <  2:2063121/100‑3 (MQ=38)
                    gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGCGCAAGTCACTGACCTg                                                                                        >  2:3393244/1‑92 (MQ=255)
                     atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGGGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCg                                                                                    <  2:2546265/95‑1 (MQ=255)
                      tttatttaCTGGAGCAGGCTCGCAAAGTAGAATGCGCAACGAGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGttttt                                                                               <  2:1558739/99‑4 (MQ=37)
                      tttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCGGGTGAt                                                                               >  2:2097806/1‑99 (MQ=255)
                      tttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtt                                                                              >  1:1317971/1‑100 (MQ=255)
                      tttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtt                                                                              >  2:3648395/1‑100 (MQ=255)
                      tttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtt                                                                              >  1:552842/1‑100 (MQ=255)
                        tatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGttttttta                                                                            <  1:2656641/100‑7 (MQ=37)
                        tatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtttt                                                                            >  1:2185658/1‑100 (MQ=255)
                        tatgtTGTGGAGCAGGCTGGCATAGTAGAATGCGCAACGCGGCCACGGTGTGGAGAATGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGttt                                                                                 <  2:3319940/95‑2 (MQ=21)
                         atttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTc                                                                           >  1:776363/1‑100 (MQ=255)
                         atttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTc                                                                           >  2:2699696/1‑100 (MQ=255)
                          ttttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCa                                                                          <  1:1177811/100‑1 (MQ=255)
                          ttttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCa                                                                          >  2:2637580/1‑100 (MQ=255)
                           tttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCag                                                                         <  2:387468/100‑1 (MQ=255)
                           tttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCag                                                                         <  2:2917214/100‑1 (MQ=255)
                            ttCTGGAGCAGACTCGCAAAGTAGAAGGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCaga                                                                        <  2:413967/100‑1 (MQ=255)
                             tCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCagag                                                                       >  2:1880606/1‑100 (MQ=255)
                               tGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAgaga                                                                      <  1:631605/99‑1 (MQ=255)
                                ggAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAg                                                                    >  1:2721629/1‑100 (MQ=255)
                                  aGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAggg                                                                  <  2:2185645/100‑1 (MQ=255)
                                  aGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAggg                                                                  <  1:3688470/100‑1 (MQ=255)
                                    cAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGa                                                                <  1:4012047/100‑1 (MQ=255)
                                      gACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAAt                                                              >  1:2936931/1‑100 (MQ=255)
                                       aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAAtt                                                             <  1:1695466/100‑1 (MQ=255)
                                       aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAAtt                                                             <  2:475017/100‑1 (MQ=255)
                                        cTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTa                                                            >  1:3357507/1‑100 (MQ=255)
                                        cTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTa                                                            >  2:2044845/1‑100 (MQ=255)
                                        cTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTa                                                            >  1:279501/1‑100 (MQ=255)
                                        cTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTa                                                            >  2:2913931/1‑100 (MQ=255)
                                        cTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTa                                                            >  1:2561425/1‑100 (MQ=255)
                                         tCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAg                                                           <  1:3320942/100‑1 (MQ=255)
                                         tCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAg                                                           <  2:371095/100‑1 (MQ=255)
                                             aaaGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAca                                                       <  2:320157/100‑1 (MQ=255)
                                              aaGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcag                                                      <  1:3904351/100‑1 (MQ=255)
                                               aGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagc                                                     <  1:1032566/100‑1 (MQ=255)
                                                gTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagca                                                    <  1:1651988/100‑1 (MQ=255)
                                                     aTGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACgg                                               >  1:2702197/1‑100 (MQ=255)
                                                     aTGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACgg                                               >  1:1298405/1‑100 (MQ=255)
                                                       gcgcAACGCGGCAACGGTGTGGAGAAGGGTTGACGAAACCGGGTAAGTAGGTAACTTGCCGGTGGATTTTCAGAGAGGGGAAATTAGTAAAGCAGAggg                                               >  2:1408154/1‑99 (MQ=255)
                                                       gcgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgc                                             >  1:925233/1‑100 (MQ=255)
                                                          cAACGCGGCAACGGTGTGGAGAAGGGATAAAACAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGcgcg                                          >  2:1292373/1‑100 (MQ=255)
                                                          cAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGcgcg                                          >  1:3775994/1‑100 (MQ=255)
                                                             cgcgGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAAt                                       <  1:2379151/100‑1 (MQ=255)
                                                              gcgGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATg                                      <  1:1604742/100‑1 (MQ=255)
                                                               cgGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATgg                                     <  2:297754/100‑1 (MQ=255)
                                                                   aaCGTTGTGGAGAAGGGATAAAAAAACGGGAACGTCGGTGACCTGCCCGGTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTAcc                                 <  2:658719/100‑1 (MQ=255)
                                                                         gtgGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAAt                           >  2:24765/1‑100 (MQ=255)
                                                                           ggAGAAGGGATAAAACAACGGGCAAGTCAGGGACCTGACCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAg                         >  2:2204609/1‑100 (MQ=255)
                                                                           ggAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAg                         <  2:3945483/100‑1 (MQ=255)
                                                                           ggAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAg                         <  2:600911/100‑1 (MQ=255)
                                                                           ggAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAg                         >  1:2067769/1‑100 (MQ=255)
                                                                            gagaAGGGATAAAAAAGCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAAGGGAATTAGTACAGCAGACGTGCGCGAATGGTACCTGGAATAGc                        >  2:887249/1‑100 (MQ=255)
                                                                            gagaAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGGAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAgn                        <  1:531322/100‑2 (MQ=255)
                                                                             agaAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCt                       <  1:1158172/100‑1 (MQ=255)
                                                                                aGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTc                    <  1:314470/100‑1 (MQ=255)
                                                                                 gggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCa                   <  1:1213315/100‑1 (MQ=255)
                                                                                  ggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCAt                  <  1:2608691/100‑1 (MQ=255)
                                                                                    aTAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTg                >  2:518027/1‑100 (MQ=255)
                                                                                    aTAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTg                >  1:1254025/1‑100 (MQ=255)
                                                                                     tAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGc               >  1:2865837/1‑100 (MQ=255)
                                                                                     tAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGc               <  2:2417638/100‑1 (MQ=255)
                                                                                       aaaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc              >  1:2684899/1‑100 (MQ=255)
                                                                                       aaaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc              <  1:1006550/100‑1 (MQ=255)
                                                                                        aatcaaCGGGCAGGTCACTGACTTGCCCGTTTATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCt             >  1:232392/5‑100 (MQ=37)
                                                                                        aaaaaaCGGGCAAGTCGTTGATCTGCCCGGGGAGCTTCAGAGAAGGGGAAGTAGTACAGCAGGCGGGCGCGAATGGTACCTGGAATAGCCTTCATTg                <  2:1420494/97‑1 (MQ=255)
                                                                                        aaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCt             >  2:3052909/1‑100 (MQ=255)
                                                                                           caaCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCa          >  1:214139/2‑100 (MQ=38)
                                                                                            aaCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAg         >  1:2502680/1‑100 (MQ=39)
                                                                                            aaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAg         <  2:2002683/100‑1 (MQ=255)
                                                                                             aCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGTAGTTAGTACAGCAGACGGGCGCGAATGGTACCGGGAATAGCGTTCATTTCCTGCAgc        >  2:343584/1‑100 (MQ=37)
                                                                                             aCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgc        >  1:463493/1‑100 (MQ=39)
                                                                                              cGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgcg       <  2:2163714/100‑1 (MQ=255)
                                                                                               gggCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgcgc      >  1:3475002/1‑100 (MQ=255)
                                                                                                 gCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCtt    >  1:2979574/1‑100 (MQ=255)
                                                                                                 gCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCGTTCATTGCCTGCAGCGCtt    >  2:311473/1‑100 (MQ=255)
                                                                                                  naaGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGCCGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCttt   >  1:3489966/2‑100 (MQ=255)
                                                                                                  cAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCttt   >  1:1854569/1‑100 (MQ=255)
                                                                                                   aaGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGCATAGCTTTCATTGCCTGCAGCGCtttt  >  2:1649543/1‑100 (MQ=255)
                                                                                                   aaGTCAGTGACCTGCCCGTTGATTGTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCtttt  >  2:2308417/1‑100 (MQ=255)
                                                                                                    |                                                                                                  
CAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTT  >  NC_000913/3057041‑3057238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: