Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,067,320 A→T 15.7% intergenic (‑147/+20) ygfI ← / ← yggE putative DNA‑binding transcriptional regulator/oxidative stress defense protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,067,3200AT15.7% 453.3 / 69.3 178intergenic (‑147/+20)ygfI/yggEputative DNA‑binding transcriptional regulator/oxidative stress defense protein
Reads supporting (aligned to +/- strand):  ref base A (66/84);  new base T (12/16);  total (78/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01

Only 100 of 193 total aligned reads displayed.

CCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACT  >  NC_000913/3067221‑3067419
                                                                                                   |                                                                                                   
ccTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCACTATCGGGTAAAGGCATTAGCCcgacga                                                                                                     >  2:1043015/1‑100 (MQ=255)
ccTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCcgacga                                                                                                     >  1:3600279/1‑100 (MQ=255)
 cTTGATGAAACCGCGAGCAAAGGTAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAAGATCGGGTAAAGGCATTAGCCCGACGaa                                                                                                    >  1:2250244/1‑100 (MQ=255)
  ttGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAAt                                                                                                   >  1:1019399/1‑100 (MQ=255)
    gatgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATAc                                                                                                 >  1:1016317/1‑100 (MQ=255)
      tgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGt                                                                                               >  1:273307/1‑100 (MQ=255)
      tgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGt                                                                                               >  1:3749329/1‑100 (MQ=255)
      tgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGt                                                                                               <  1:570794/100‑1 (MQ=255)
         aaCCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCgg                                                                                            <  1:970120/100‑1 (MQ=255)
         aaCCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCgg                                                                                            <  1:1273683/100‑1 (MQ=255)
          aCCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCggg                                                                                           <  1:368070/100‑1 (MQ=255)
          aCCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGc                                                                                          <  1:2295004/100‑1 (MQ=255)
           ccGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGc                                                                                          <  1:3374452/100‑1 (MQ=255)
           ccGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGc                                                                                          <  2:1153452/100‑1 (MQ=255)
            cgcgAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGc                                                                                          <  2:3311174/99‑1 (MQ=255)
            cgcgAGAAAATATAGGTGATTACGTCATGGTTTTTCAGAAAATTACAGAAACAGGAGGCAATATCGGTTAAAGGCATTAGCCCGACGTATTCGTCggg                                                                                           <  2:4126756/98‑1 (MQ=255)
             gcgAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGc                                                                                          <  2:1797879/98‑1 (MQ=255)
             gcgAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAAGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGc                                                                                          >  2:2218302/1‑98 (MQ=255)
                aGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGCTaatg                                                                                     <  1:3230627/100‑4 (MQ=39)
                 tcAACGATAGGTGATCACGTCATCGTCTTACAGAAATTTACAGAAAAAGGAGGAAACAACGGGTAAAGGCATTACCCCGACGAATAAGTCGCGCTACaa                                                                                     >  2:3666469/2‑99 (MQ=255)
                  cAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCAGTAGCCCGACGTATTCGTCGGGCTaatgcc                                                                                   <  1:3418036/100‑6 (MQ=38)
                   naaGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAata                                                                                  >  2:882432/2‑100 (MQ=255)
                   aaaGTTATGTGATTACGTCATGGTTTTTCAGAAAATTACAGAAAAAGGAGGCAATATCGGCTAAAGGCATTAGCCCGACGAATACGTCGGGCTAc                                                                                       <  2:3413273/95‑1 (MQ=255)
                    aaGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAatat                                                                                 <  1:3003557/100‑1 (MQ=255)
                         aGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATtg                                                                            <  1:2122622/100‑1 (MQ=255)
                         aGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATtg                                                                            <  1:3506120/100‑1 (MQ=255)
                          ggTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATattatt                                                                             <  2:3160210/98‑1 (MQ=255)
                            tGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgc                                                                         <  2:961074/100‑1 (MQ=255)
                              aTTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgctg                                                                       <  2:3985449/100‑1 (MQ=255)
                                tACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCa                                                                     >  2:1269590/1‑100 (MQ=255)
                                tACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCa                                                                     <  2:1055775/100‑1 (MQ=255)
                                 aCGTGATGGTTTTACAGAAAATTAAAGAAAAAGGAGGCAATATCGGGTAAATGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAg                                                                    <  2:3771016/100‑1 (MQ=255)
                                  cGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAgg                                                                   <  1:555805/100‑1 (MQ=255)
                                  cGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAgg                                                                   <  2:4106707/100‑1 (MQ=255)
                                    tCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGgtg                                                                 >  1:4043919/1‑100 (MQ=255)
                                       tGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGttt                                                              >  1:1282072/1‑100 (MQ=255)
                                            ttacagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCg                                                         <  2:1638442/100‑1 (MQ=255)
                                             tacagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCgg                                                        >  2:286902/1‑100 (MQ=255)
                                             tacagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCgg                                                        <  2:272967/100‑1 (MQ=255)
                                             tacagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTAAAAATATTACTGTGCTGCAGGTGTTTTAGCgg                                                        >  2:2788978/1‑100 (MQ=255)
                                              gcagcctattacagaaaaTGGAGGCAATATCGGGTAAACGCATTAGTCCGAAGAAAACGTATGGCTACAAAAATTATTGTGCCGCACGTGTTTTAGCg                                                         >  2:2472415/8‑98 (MQ=255)
                                              acagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCggg                                                       <  2:6804/100‑1 (MQ=255)
                                              acagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCggg                                                       >  2:2956065/1‑100 (MQ=255)
                                               cagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTa                                                            >  2:253510/1‑94 (MQ=255)
                                               cagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgt                                                      >  1:1038679/1‑100 (MQ=255)
                                               cagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgt                                                      >  2:3012653/1‑100 (MQ=255)
                                               cagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgt                                                      >  1:1421492/1‑100 (MQ=255)
                                                 gaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgttg                                                    <  1:704409/100‑1 (MQ=255)
                                                  aaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgttgt                                                   <  2:362477/100‑1 (MQ=255)
                                                   aaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgttgtt                                                  <  1:2889705/100‑1 (MQ=255)
                                                    aattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGttgttg                                                 >  1:1503249/1‑100 (MQ=255)
                                                     attacagaaaaAGGATGCAATATCTGGTAAAGGCATTAGCCCGACGATTACGTTGGGATACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGttgttg                                                 <  2:1098092/99‑1 (MQ=255)
                                                     attacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGa                                                <  2:466110/100‑1 (MQ=255)
                                                      ttacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGAt                                               <  1:2457777/100‑1 (MQ=255)
                                                        ncagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATcc                                             >  1:844058/2‑100 (MQ=255)
                                                        acagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATcc                                             <  2:1435542/100‑1 (MQ=255)
                                                         cagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGAtgc                                             >  2:3481492/1‑97 (MQ=255)
                                                         cagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCca                                            >  1:3202784/1‑100 (MQ=255)
                                                         cagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCca                                            >  1:3287918/1‑100 (MQ=255)
                                                           gaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCcaca                                          <  2:2483054/100‑1 (MQ=255)
                                                           gaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCcaca                                          >  1:270600/1‑100 (MQ=255)
                                                           gaaaaAGGAGGCAATATCGGGTAAAGGCATGAGCCCGACGAATGCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCcaca                                          <  2:1889479/100‑1 (MQ=255)
                                                             aaaaGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAgg                                        <  1:3974848/100‑1 (MQ=255)
                                                             aaaaGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAgg                                        >  1:3253658/1‑100 (MQ=255)
                                                              aaaGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGt                                       >  1:1807848/1‑100 (MQ=255)
                                                               aaGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGtt                                      <  1:882438/100‑1 (MQ=255)
                                                                 ggaggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGATGTTGATCCACAGGTTCt                                    >  2:3840215/1‑100 (MQ=255)
                                                                    ggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAAc                                 >  1:2416042/1‑100 (MQ=255)
                                                                        atatCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGa                             <  2:1330244/100‑1 (MQ=255)
                                                                        atatCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGa                             <  1:3096851/100‑1 (MQ=255)
                                                                          atCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAg                           <  1:1218701/100‑1 (MQ=255)
                                                                           tCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGa                          <  2:202365/100‑1 (MQ=255)
                                                                           tCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGATTGTTGATCCACAGGTTCTAACTGGAAGa                          >  2:1490491/1‑100 (MQ=255)
                                                                            cGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGAc                         <  1:1559366/100‑1 (MQ=255)
                                                                             gggTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTGACTGGAAGAcc                        <  1:196550/100‑1 (MQ=255)
                                                                              ggTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGAcc                        >  1:3934827/1‑99 (MQ=255)
                                                                              ggTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACca                       <  2:12871/100‑1 (MQ=255)
                                                                              ggTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACca                       <  2:3077536/100‑1 (MQ=255)
                                                                                tAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACcaca                     >  1:2866951/1‑100 (MQ=255)
                                                                                  aaGGCATTAGCCCGACGAATACGTCTGGCTACAAATATTATTGTGCTGCAGGTGTGTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATc                   <  2:1580250/100‑1 (MQ=255)
                                                                                    ggCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCg                  >  2:4029691/1‑99 (MQ=255)
                                                                                       tgtAGCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCt              >  1:3784668/3‑100 (MQ=255)
                                                                                       aTTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCt              >  1:1241226/1‑100 (MQ=255)
                                                                                          aGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGat           >  2:100552/1‑100 (MQ=255)
                                                                                          aGCCCGACGAATACGTCGGGCTACAAATATTATTCTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGat           <  1:1322434/100‑1 (MQ=255)
                                                                                           ggccGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatc          >  1:2806719/3‑100 (MQ=255)
                                                                                           gCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatc          >  1:3173148/1‑100 (MQ=255)
                                                                                           gCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatc          >  2:2061752/1‑100 (MQ=255)
                                                                                           gCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatc          >  1:1688459/1‑100 (MQ=255)
                                                                                           gCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatc          >  1:483836/1‑100 (MQ=255)
                                                                                           gCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatc          <  1:1984089/100‑1 (MQ=255)
                                                                                            cccGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatca         <  1:2061765/100‑1 (MQ=255)
                                                                                            cccGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatca         <  2:123524/100‑1 (MQ=255)
                                                                                            cccGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatca         <  1:2410606/100‑1 (MQ=255)
                                                                                               gacgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGAtcatca      >  1:2869408/1‑100 (MQ=255)
                                                                                                 cgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGAtcatcaga    <  1:2792519/100‑3 (MQ=255)
                                                                                                 cgaATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCAACAGATTCTAACTGGAAGACCACATCGACCTGATCATCaaa    >  2:3771874/1‑100 (MQ=255)
                                                                                                   aaTACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACt  >  1:2627150/1‑100 (MQ=255)
                                                                                                   aaTACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACt  >  2:1863899/1‑100 (MQ=255)
                                                                                                   aaTACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACt  <  2:2063261/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACT  >  NC_000913/3067221‑3067419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: