Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,118,566 C→T 55.6% A183T (GCG→ACG)  sslE ← putative secreted and surface‑associated lipoprotein mucinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,118,5660CT55.6% 38.5 / 190.4 151A183T (GCG→ACG) sslEputative secreted and surface‑associated lipoprotein mucinase
Reads supporting (aligned to +/- strand):  ref base C (25/42);  new base T (38/46);  total (63/88)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

Only 100 of 153 total aligned reads displayed.

TGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACT  >  NC_000913/3118467‑3118663
                                                                                                   |                                                                                                 
tGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGc                                                                                                   >  2:3208921/1‑100 (MQ=255)
  aaCTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCg                                                                                                 <  1:3850827/100‑1 (MQ=255)
   aCTCTTCCTGTGCCAGATCGTTTTCTTTATACAGCGAGTTGAAGCGTTTACTCTCGATCATCGTGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCgg                                                                                                <  2:4117637/100‑1 (MQ=255)
   aCTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCgg                                                                                                >  2:680126/1‑100 (MQ=255)
    ctctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGa                                                                                               >  1:1527807/1‑100 (MQ=255)
     tctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGAc                                                                                              <  2:3530628/100‑1 (MQ=255)
     tctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGAc                                                                                              <  1:1272237/100‑1 (MQ=255)
      ctTCCGGTGCCAGATCTATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACa                                                                                             <  2:1300641/100‑1 (MQ=255)
      ctTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACa                                                                                             <  1:3002906/100‑1 (MQ=255)
        tCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGc                                                                                           >  1:829484/1‑100 (MQ=255)
         ccGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTTTATTCGTCGGACAGCt                                                                                          >  2:101738/1‑100 (MQ=255)
          cGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTg                                                                                         <  1:1324000/100‑1 (MQ=255)
            gTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGtt                                                                                       <  1:217557/100‑1 (MQ=255)
             tGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTGGCCGGACAGCTGTTa                                                                                      >  1:4064129/1‑100 (MQ=255)
              gCCTGATCGAGTTGCTTATGCAGGGAGTGGAAGCGTTTGCTCTGGATCACCGGGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGtt                                                                                       <  2:3412358/98‑1 (MQ=255)
                cAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTg                                                                                   <  2:2830743/100‑1 (MQ=255)
                 aGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTgg                                                                                  >  1:3853158/1‑100 (MQ=255)
                 aGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTgg                                                                                  <  2:2332513/100‑1 (MQ=255)
                  gATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGa                                                                                 >  2:411839/1‑100 (MQ=255)
                  gATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGa                                                                                 >  1:3172336/1‑100 (MQ=255)
                    tCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACg                                                                               <  1:267093/100‑1 (MQ=255)
                       aTTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACCGCTCTTACTGGACGTTa                                                                            >  2:1946209/1‑100 (MQ=255)
                         ttGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTAcc                                                                          <  2:764828/100‑1 (MQ=255)
                         ttGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTAcc                                                                          <  1:3095558/100‑1 (MQ=255)
                          tGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCa                                                                         >  1:3796241/1‑100 (MQ=255)
                           gCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAg                                                                        >  1:3732750/1‑100 (MQ=255)
                           gCTTATACAGCGAGTCGAAGCGTTTACGCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAg                                                                        <  2:777080/100‑1 (MQ=255)
                            cTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGc                                                                       >  2:1010671/1‑100 (MQ=255)
                            cTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGc                                                                       <  1:1713267/100‑1 (MQ=255)
                            cTTATACAGCGAGTCGAAGCGTTTACACTCGATCACCGAGGAGAACGTCAGAAAAACCTGTTCTGTATTCGTCGGACAGCTGTCACTGTACGTTACCAg                                                                        >  2:1494174/1‑99 (MQ=255)
                              tataCAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCg                                                                      <  2:541343/99‑1 (MQ=255)
                                taCAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGaaa                                                                   >  2:1736020/1‑100 (MQ=255)
                                 aCAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAAc                                                                  <  2:1639261/100‑1 (MQ=255)
                                  cAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAAcc                                                                 >  2:2609152/1‑100 (MQ=255)
                                  cAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAAcc                                                                 >  1:333180/1‑100 (MQ=255)
                                  cAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGCCAGCTGTTACTGGACGTTACCAGCGAAAcc                                                                 >  1:2627930/1‑100 (MQ=255)
                                  cAGCGAGTCGAAGCGTTTACTCTCGATCAACGAGGCGAACGTCAGACAAACCTGTTCTGTATTCGCTGGACAGCTGTTACTGGACGTTACCAGCGAAAcc                                                                 >  2:1868184/1‑100 (MQ=255)
                                   aGCGAGTGGAAACGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCg                                                                >  2:2161719/1‑100 (MQ=255)
                                   aGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAAcn                                                                 <  2:3948001/99‑2 (MQ=255)
                                    gCGAGTCGAAGTGTTTACTCTCGTTCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGc                                                               <  2:1362663/100‑1 (MQ=255)
                                     cGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTGACTGGACGTTACCAGCGAAACCGCa                                                              <  2:205667/100‑1 (MQ=255)
                                      gAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCAt                                                             >  1:4017109/1‑100 (MQ=255)
                                       aGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCAtt                                                            >  1:15759/1‑100 (MQ=255)
                                        gtcgaattttttACTCTCGATCACTGGGGAGAACGTCAGACAATCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCAtt                                                            <  2:3166034/90‑1 (MQ=255)
                                        gTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTg                                                           >  1:2216370/1‑100 (MQ=255)
                                           gtagccgttgctctCCAGCACCGGGGAGAACGCGAGACAAACCTGTTCTGTATTCGTCGGACATCTGTTAGTGGACGGCACCAGCGAAACCGCATTGCtt                                                        <  2:3927568/90‑1 (MQ=255)
                                           gAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCtt                                                        >  1:2448551/1‑100 (MQ=255)
                                            aaGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCttt                                                       >  1:3130414/1‑100 (MQ=255)
                                             attgTTTCCTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTTTATTGGTCGGACACCTGTTACTGGACTTTACCAGCGAAACCTCATTGCt                                                         <  2:2685079/94‑1 (MQ=255)
                                             aGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCtttt                                                      <  2:2339089/100‑1 (MQ=255)
                                                gTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCtt                                                   <  1:476802/100‑1 (MQ=255)
                                                 tttACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTg                                                  <  1:4091207/100‑1 (MQ=255)
                                                 tttACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTg                                                  <  2:586753/100‑1 (MQ=255)
                                                 tttACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTg                                                  <  1:3287786/100‑1 (MQ=255)
                                                   tACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTc                                                <  2:835134/100‑1 (MQ=255)
                                                     ctctCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACCGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCAt                                              >  2:2025222/1‑100 (MQ=255)
                                                      tctcGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATc                                             <  2:1983049/100‑1 (MQ=255)
                                                       ctcGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATc                                             >  1:1017767/1‑99 (MQ=255)
                                                        tcGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCgg                                           <  2:4093355/100‑1 (MQ=255)
                                                        tcGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCgg                                           <  1:3702270/100‑1 (MQ=255)
                                                        tcGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCgg                                           <  2:3869111/100‑1 (MQ=255)
                                                         cGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGa                                          >  2:3921091/1‑100 (MQ=255)
                                                          gATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAg                                         <  2:2980131/100‑1 (MQ=255)
                                                          gATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAg                                         <  1:2398053/100‑1 (MQ=255)
                                                          gATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAg                                         >  1:2463995/1‑100 (MQ=255)
                                                          gATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAg                                         <  1:680132/100‑1 (MQ=255)
                                                          gATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAg                                         <  1:3483601/100‑1 (MQ=255)
                                                          gATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAg                                         <  1:3156313/100‑1 (MQ=255)
                                                           aTCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGc                                        >  2:2568147/1‑100 (MQ=255)
                                                              aCCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCg                                     >  2:1785210/1‑100 (MQ=255)
                                                              aCCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCg                                     >  2:610341/1‑100 (MQ=255)
                                                              aCCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCg                                     <  1:353815/100‑1 (MQ=255)
                                                                cGAGGAGAACGTCAGACAAATCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGcgcccgcc                                   <  2:2139200/100‑1 (MQ=255)
                                                                cGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGcc                                   <  1:1036440/100‑1 (MQ=255)
                                                                cGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGcc                                   <  1:3012731/100‑1 (MQ=255)
                                                                 gaggagAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCa                                  >  2:2116440/1‑100 (MQ=255)
                                                                  aggagAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAg                                 <  2:514360/100‑1 (MQ=255)
                                                                   ggagAACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGt                                <  1:189526/100‑1 (MQ=255)
                                                                      gaACGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCCGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCt                             >  2:2545521/1‑100 (MQ=255)
                                                                         cGTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgc                          <  2:1843405/100‑1 (MQ=255)
                                                                         cGTCAGACAAACCTGTTCGGTCTTCGCCGGACAGATCTCACTGGTCGTCACCAACGAAACCGCATTGCTTTTCCTGTCATCCGACCCCGCCAGTTCttcc                          <  2:3419882/100‑3 (MQ=255)
                                                                          gTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg                         <  1:1999775/100‑1 (MQ=255)
                                                                          gTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg                         <  1:2127903/100‑1 (MQ=255)
                                                                          gTCAGACAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTgcg                         <  1:1231102/100‑1 (MQ=255)
                                                                          gTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGACCCCGCCAGTTCTTgcg                         <  2:1216947/100‑1 (MQ=255)
                                                                               ataaaCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTcc                    <  2:2508475/98‑1 (MQ=255)
                                                                               aCAAACTTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCACCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTcc                    <  2:66642/100‑1 (MQ=255)
                                                                               aCAAACCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTcc                    <  1:1877293/100‑1 (MQ=255)
                                                                               aCAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTcc                    <  1:637900/100‑1 (MQ=255)
                                                                                 aaaCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTc                  <  1:791543/100‑1 (MQ=255)
                                                                                  aaCCTGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCa                 <  2:1625307/100‑1 (MQ=255)
                                                                                   aCCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCaa                >  1:1842506/1‑100 (MQ=255)
                                                                                     ccgTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAgg              <  2:367287/98‑1 (MQ=255)
                                                                                      tGTTCTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGc             <  1:2255842/100‑1 (MQ=255)
                                                                                          cTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCACGGCTaaa         >  2:2762295/1‑100 (MQ=255)
                                                                                          cTGTATTCGTCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTaaa         >  2:1265312/1‑100 (MQ=255)
                                                                                            gTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCTGCCAGTTCTTGCGCGTCTTCAAGGCTAAACg       <  1:2782158/100‑1 (MQ=255)
                                                                                            gTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACg       >  1:2026927/1‑100 (MQ=255)
                                                                                            gTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACg       <  1:120311/100‑1 (MQ=255)
                                                                                                tcgtcgGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGacac   >  1:2555392/1‑100 (MQ=255)
                                                                                                   |                                                                                                 
TGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCGCCGGACAGCTGTTACTGGACGTTACCAGCGAAACCGCATTGCTTTTCTTGTCATCGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACT  >  NC_000913/3118467‑3118663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: