Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,165,015 T→A 26.1% D320V (GAT→GTT)  parC ← DNA topoisomerase IV, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,165,0150TA26.1% 326.6 / 118.8 186D320V (GAT→GTT) parCDNA topoisomerase IV, subunit A
Reads supporting (aligned to +/- strand):  ref base T (59/77);  new base A (21/27);  total (82/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 189 total aligned reads displayed.

ACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCAT  >  NC_000913/3164917‑3165114
                                                                                                  |                                                                                                   
 cacCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGTCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATca                                                                                                   >  2:2262472/1‑99 (MQ=255)
 cacCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCg                                                                                                   <  2:2663709/100‑1 (MQ=255)
  acCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACgg                                                                                                  <  2:1079211/100‑1 (MQ=255)
   ccAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCTGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCggt                                                                                                 >  1:2972781/1‑100 (MQ=255)
   ccAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCggt                                                                                                 >  2:3034493/1‑100 (MQ=255)
   ccAGCCATTCGGAGAGGATTTCCAGCAGGTTTCTCACCGCCAGACGAACATACAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAAAtaaa                                                                                                  >  2:1795773/1‑96 (MQ=255)
     aGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCggtgg                                                                                               <  2:24425/100‑1 (MQ=255)
     aGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCggtgg                                                                                               <  2:19365/100‑1 (MQ=255)
      gCCATTCGGAGAGGATTTCTAGCATGTTTTTCACAGGCGGACGGCCATGCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCgttagt                                                                                              <  2:4111935/100‑6 (MQ=255)
        cATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAg                                                                                            <  2:4013148/100‑1 (MQ=255)
        cATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAg                                                                                            <  1:447867/100‑1 (MQ=255)
          ttCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCg                                                                                          <  1:1082572/100‑1 (MQ=255)
             ggAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAag                                                                                       >  2:3801395/1‑100 (MQ=255)
              gagaGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAaga                                                                                      >  1:1008694/1‑100 (MQ=255)
               agagGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGagaaga                                                                                      >  2:652174/1‑99 (MQ=255)
                  ggATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGCggtg                                                                                  >  1:3947067/1‑100 (MQ=255)
                    aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCGGATCGGTGGTAGCGAAGAggtgg                                                                                 >  1:1538188/1‑99 (MQ=255)
                    aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAggtggt                                                                                <  1:607635/100‑1 (MQ=255)
                    aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtggt                                                                                <  1:1388472/100‑1 (MQ=255)
                     tttCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGtt                                                                               >  1:3314247/1‑100 (MQ=255)
                      ttCCAGCATGTTTTTCATCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtc                                                                              <  2:1455259/100‑1 (MQ=255)
                       tCCAGCAGGTTTTTCACCGCCGGACGCCCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAaga                                                                                      >  2:3347934/1‑91 (MQ=255)
                        ccAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtcat                                                                            >  2:883249/1‑100 (MQ=255)
                        ccAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcat                                                                            >  1:2947218/1‑100 (MQ=255)
                         cagcagGTTTTTCACCGCCGGACGATCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtcatc                                                                           >  1:472729/1‑100 (MQ=255)
                         cagcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtcatc                                                                           >  1:3761210/1‑100 (MQ=255)
                         cagcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcacc                                                                           <  1:2802359/100‑3 (MQ=255)
                          agcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtcatca                                                                          <  1:2049231/100‑1 (MQ=255)
                            cagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCAcc                                                                        <  2:2572230/100‑1 (MQ=255)
                             agGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCt                                                                       <  1:1915295/100‑1 (MQ=255)
                             aagTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCAcc                                                                        <  2:2002346/97‑1 (MQ=255)
                              ggTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTg                                                                      >  1:1216786/1‑100 (MQ=255)
                               gtgtttCATCGCGGGACGACCATCCAGACCGATCATATGAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCAc                                                                         <  2:2803014/93‑1 (MQ=255)
                                tttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGAt                                                                    <  2:3047224/100‑1 (MQ=255)
                                 ttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATc                                                                   <  1:788165/100‑1 (MQ=255)
                                 ttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATc                                                                   <  2:930/100‑1 (MQ=255)
                                  tttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATcc                                                                  <  2:1915738/100‑1 (MQ=255)
                                  tttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATcc                                                                  >  1:1774635/1‑100 (MQ=255)
                                  tttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTGCATCACCTGATcc                                                                  >  2:3464378/1‑100 (MQ=255)
                                   ttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCa                                                                 <  1:4018883/100‑1 (MQ=255)
                                   ttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCa                                                                 <  1:3254171/100‑1 (MQ=255)
                                      aCCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTATTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCatat                                                              >  2:2939025/1‑100 (MQ=255)
                                         ggcgtacgagCATCCAGACCTATCATATTAAGGTTAATGCGATGGCTCTTTTCCAGTTCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCata                                                               <  1:2235977/91‑1 (MQ=255)
                                         gccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCa                                                           <  1:873163/100‑1 (MQ=255)
                                         gccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCa                                                           <  1:1150406/100‑1 (MQ=255)
                                               cgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGt                                                     <  1:3381871/100‑1 (MQ=255)
                                               cgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGt                                                     >  1:2357531/1‑100 (MQ=255)
                                               cgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGt                                                     <  2:3971783/100‑1 (MQ=255)
                                                 acCATCCAGACCGATCATATTACGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTg                                                   >  2:1286554/1‑100 (MQ=255)
                                                 acCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTg                                                   >  2:2654222/1‑100 (MQ=255)
                                                 acCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTg                                                   <  1:1594226/100‑1 (MQ=255)
                                                  ccATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTgg                                                  <  1:2134098/100‑1 (MQ=255)
                                                   cATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGa                                                 <  2:472725/100‑1 (MQ=255)
                                                   cATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTAATCACCTGATCCATATCCACGCGGTTGGa                                                 >  1:1373702/1‑100 (MQ=255)
                                                     tCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAc                                               <  2:2779183/100‑1 (MQ=255)
                                                       cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAcgc                                             <  1:2658508/100‑1 (MQ=255)
                                                       cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAcgc                                             >  2:3323167/1‑100 (MQ=255)
                                                       cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAcgc                                             >  1:476484/1‑100 (MQ=255)
                                                       cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAcgc                                             >  2:531888/1‑100 (MQ=255)
                                                        aGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAcgcg                                            <  2:620580/100‑1 (MQ=255)
                                                          aCCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGc                                          <  1:360487/100‑1 (MQ=255)
                                                           ccGATCATATTAAGGTTAATACGATAGCTCTTTTGCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACTTGATCCATATCCACGCGGTTGGAACGTGGca                                         <  2:3365648/100‑1 (MQ=255)
                                                              aTCATATTAAGGTTAATACGATAGCTCTTTTCCTGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGCAACGCGGcaca                                       <  2:2167075/99‑1 (MQ=255)
                                                                cATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATc                                    >  1:1766693/1‑100 (MQ=255)
                                                                 atatTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCa                                   >  2:1140023/1‑100 (MQ=255)
                                                                  tatTAAGCTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAGCGCGGCACAATCAc                                  >  1:2174729/1‑100 (MQ=255)
                                                                   atTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAcc                                 >  1:1343613/1‑100 (MQ=255)
                                                                   atTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAc                                  >  2:2757532/1‑99 (MQ=255)
                                                                    ttAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAcc                                 >  1:2583204/1‑99 (MQ=255)
                                                                    ttAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCa                                >  1:3395318/1‑100 (MQ=255)
                                                                    ttAAGGTTAATACGATAGCGCTTTTCCAGAAAGGTGGTAGCGAAGAGGTGGTTCATCACCTTATCCATATACACGCGGTTGGAACGCGGCACAATc                                    >  2:367489/1‑96 (MQ=255)
                                                                      aaGGTTAATACGATAGCTCTTTTCCAGGACGGTGGTAGCGAAGAGGGGGTTCATCACCTGATCCATGTCCACGCGGTTGGAACGCGGCACAATCACCAgg                              <  2:2612037/100‑1 (MQ=255)
                                                                         gTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgg                           <  1:1706583/100‑1 (MQ=255)
                                                                         gTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgg                           >  2:1670845/1‑100 (MQ=255)
                                                                          ttAATACGATAGCTCTTTTCCAGATCGGTCATAGCGAAGAGGAGGTTCATCGCCAGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcggn                          <  2:2046950/100‑2 (MQ=255)
                                                                              tACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTTATCACCTGATTCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcggat                         >  2:1272785/1‑95 (MQ=255)
                                                                              tACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcgg                      <  2:1020802/100‑1 (MQ=255)
                                                                               aCGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcggg                     <  2:2047153/100‑1 (MQ=255)
                                                                                cGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcgggt                    >  1:1026543/1‑100 (MQ=255)
                                                                                   tAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGGTGGAACGCGGCACAATCACCAGGCGGGTCGGGTtct                 >  1:3654414/1‑100 (MQ=255)
                                                                                    aGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTtctc                <  1:1316823/100‑1 (MQ=255)
                                                                                     gCTCTTTTCCAGATCGGTGGTAGCGCAGAGGTGGTTCATCACCTGATCCATATCCACGAGGTTGGAACGCGGCACACTCACCAGGCGGGTCGGGTTCTCg               >  1:843023/1‑100 (MQ=255)
                                                                                     gCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCg               >  1:1646065/1‑100 (MQ=255)
                                                                                     gCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCg               >  2:4020480/1‑100 (MQ=255)
                                                                                     gCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCg               <  1:3801419/100‑1 (MQ=255)
                                                                                      ctctTTTTCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGCAACGCGGCACAATCACCAGGCGGGTCGTGTTCTCGt              >  2:3231172/1‑100 (MQ=255)
                                                                                      ctctTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCAGCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGt              >  2:2210074/1‑100 (MQ=255)
                                                                                      ctctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGt              >  1:3167460/1‑100 (MQ=255)
                                                                                       tctTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTg             >  2:2703015/1‑100 (MQ=255)
                                                                                        ctTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTg             >  1:2091983/1‑99 (MQ=255)
                                                                                          tttCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTc          <  1:3307698/100‑1 (MQ=255)
                                                                                          tttCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATACACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTc          >  1:2459966/1‑100 (MQ=255)
                                                                                           ttCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCa         >  1:2086553/1‑100 (MQ=255)
                                                                                             ccAGATCGGTGGTAGCGAAGAGGGGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAg        >  2:2182284/1‑99 (MQ=255)
                                                                                             ccAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGa       >  1:2722637/1‑100 (MQ=255)
                                                                                               aGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAtt     >  2:371783/1‑100 (MQ=255)
                                                                                               aGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAtt     <  1:195359/100‑1 (MQ=255)
                                                                                                gATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTc    >  2:767131/1‑100 (MQ=255)
                                                                                                  tCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCAt  <  2:1621885/100‑1 (MQ=255)
                                                                                                  tCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCAt  <  1:2714951/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
ACACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGATTCAT  >  NC_000913/3164917‑3165114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑