Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,244,928 T→C 51.5% intergenic (‑187/‑296) uxaC ← / → exuT uronate isomerase/hexuronate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,244,9280TC51.5% 34.2 / 265.7 201intergenic (‑187/‑296)uxaC/exuTuronate isomerase/hexuronate transporter
Reads supporting (aligned to +/- strand):  ref base T (49/48);  new base C (43/59);  total (93/108)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 201 total aligned reads displayed.

TTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGA  >  NC_000913/3244831‑3245026
                                                                                                 |                                                                                                  
 tCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAAt                                                                                                 >  2:729535/1‑100 (MQ=255)
    tGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTc                                                                                              <  1:73871/100‑1 (MQ=255)
    tGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCGGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTc                                                                                              >  2:2648608/1‑100 (MQ=255)
     gAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTcc                                                                                             >  1:514811/1‑100 (MQ=255)
      aGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTccc                                                                                            <  2:840388/100‑1 (MQ=255)
        ttAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTa                                                                                          <  2:3601743/100‑1 (MQ=255)
         tAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTAc                                                                                         >  2:1702924/1‑100 (MQ=255)
         tAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTAc                                                                                         >  1:2038194/1‑100 (MQ=255)
         tAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTAc                                                                                         <  1:1515369/100‑1 (MQ=255)
         tAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTAc                                                                                         >  1:2614613/1‑100 (MQ=255)
          aGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACa                                                                                        >  2:2110391/1‑100 (MQ=255)
            aTCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGACCTTGCTGCCGTCAGGTAATGTCCCTACaaa                                                                                      >  2:2795546/1‑100 (MQ=255)
               aaTAAACGTAGTTAAAAAAATTACTCTCAAAGAGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAatat                                                                                   >  1:478789/1‑100 (MQ=255)
                aTAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATAtt                                                                                  <  1:158287/100‑1 (MQ=255)
                 tAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTc                                                                                 <  1:1894953/100‑1 (MQ=255)
                   aaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTccc                                                                               >  1:1737573/1‑100 (MQ=255)
                   aaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTccc                                                                               <  1:246695/100‑1 (MQ=255)
                   aaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTccc                                                                               >  1:1222201/1‑100 (MQ=255)
                   aaCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTccc                                                                               >  2:2566866/1‑100 (MQ=255)
                    aCGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCca                                                                              <  2:148664/100‑1 (MQ=255)
                     cGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCcac                                                                             <  1:1702936/100‑1 (MQ=255)
                     cGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCcac                                                                             >  1:1406797/1‑100 (MQ=255)
                      gTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCcaca                                                                            <  1:2207030/100‑1 (MQ=255)
                      gTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCcaca                                                                            >  2:2731381/1‑100 (MQ=255)
                        aGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACAtt                                                                          >  1:2746544/1‑100 (MQ=255)
                         gTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACAttt                                                                         >  2:1916824/1‑100 (MQ=255)
                           tAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTtgt                                                                       >  1:751952/1‑100 (MQ=255)
                            aaaaaaaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTtgtg                                                                      <  1:3032935/100‑1 (MQ=255)
                             aaaaaaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGa                                                                     <  2:3965940/100‑1 (MQ=255)
                             aaaaaaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGa                                                                     <  1:1132439/100‑1 (MQ=255)
                               aaaaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATg                                                                   <  1:685620/100‑1 (MQ=255)
                                aaaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATgg                                                                  <  1:3304836/100‑1 (MQ=255)
                                 aaTTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGc                                                                 <  1:2394693/100‑1 (MQ=255)
                                   ttACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGctc                                                               <  2:1730221/100‑1 (MQ=255)
                                   ttACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTGGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGctc                                                               <  2:856802/100‑1 (MQ=255)
                                        ctcAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCAcc                                                          >  2:2966117/1‑100 (MQ=255)
                                         ncAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAACTCTTCCCACATTTGTGATGGCTCTCACCt                                                         >  2:3722849/2‑100 (MQ=255)
                                           aaaGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCttt                                                       <  1:3730870/100‑1 (MQ=255)
                                           aaaGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCttt                                                       >  1:3536833/1‑100 (MQ=255)
                                            aaGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCtttt                                                      <  2:3325185/100‑1 (MQ=255)
                                            aaGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCtttt                                                      <  1:2772041/100‑1 (MQ=255)
                                             aGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTa                                                     <  2:1106376/100‑1 (MQ=255)
                                               tGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTaaa                                                   <  1:1997967/100‑1 (MQ=255)
                                                ggTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAg                                                  >  1:2706503/1‑100 (MQ=255)
                                                ggTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAg                                                  <  1:544326/100‑1 (MQ=255)
                                                ggTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATAGTCCCACATTTGTGATGGCTCTCACCTTTTAAAg                                                  <  2:1594687/100‑1 (MQ=255)
                                                  tAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGtt                                                >  2:295325/1‑100 (MQ=255)
                                                  tAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGtt                                                >  1:4111903/1‑100 (MQ=255)
                                                   aaaTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTg                                               <  1:96411/100‑1 (MQ=255)
                                                   aaaTCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTg                                               <  2:3929647/100‑1 (MQ=255)
                                                      tctcGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTAt                                            <  1:1451889/100‑1 (MQ=255)
                                                       ctcGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTAt                                            >  1:2254298/1‑99 (MQ=255)
                                                             gCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGt                                     <  1:721575/100‑1 (MQ=255)
                                                             gCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGt                                     <  1:2286582/100‑1 (MQ=255)
                                                             gCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGt                                     >  2:1222441/1‑100 (MQ=255)
                                                             gCAGACCGCGCCAGTACTGGCCTTGCTGTCGGCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCGTTTAAAGTTGTATGACAAGt                                     <  2:1959165/100‑1 (MQ=255)
                                                                ggCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTAt                                  >  1:4052637/1‑100 (MQ=255)
                                                                ggCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCGTTTAAAGTTGTATGACAAGTTAt                                  >  2:4092495/1‑100 (MQ=255)
                                                                ggCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAACTATTCCCACATTTGTGATGGCTCTCACCGTTTAAAGTTGTATGACAAGTTAt                                  >  2:2434312/1‑100 (MQ=255)
                                                                  ccGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCt                                >  2:584555/1‑100 (MQ=255)
                                                                   cgcgCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCtt                               >  1:3796109/1‑100 (MQ=255)
                                                                   cgcgCCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCtt                               <  1:3663075/100‑1 (MQ=255)
                                                                    gcgcCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCttt                              >  2:817372/1‑100 (MQ=255)
                                                                    gcgcCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCttt                              >  1:3671527/1‑100 (MQ=255)
                                                                     cgcCAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTc                             <  1:3914079/100‑1 (MQ=255)
                                                                      gcCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCt                            <  2:3651790/100‑1 (MQ=255)
                                                                      gcCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCt                            >  1:2938087/1‑100 (MQ=255)
                                                                       ccAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTg                           <  1:1782752/100‑1 (MQ=255)
                                                                       ccAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTg                           <  1:3361005/100‑1 (MQ=255)
                                                                       ccAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTg                           <  2:3828126/100‑1 (MQ=255)
                                                                       ccAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTg                           >  1:935425/1‑100 (MQ=255)
                                                                        cAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGc                          <  1:3648834/100‑1 (MQ=255)
                                                                        cAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGc                          <  1:2981/100‑1 (MQ=255)
                                                                        cAGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGc                          <  2:1042307/100‑1 (MQ=255)
                                                                         aGTACTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGcc                         <  1:2966134/100‑1 (MQ=255)
                                                                            aCTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTc                      <  2:1381621/100‑1 (MQ=255)
                                                                            aCTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTc                      <  1:3973415/100‑1 (MQ=255)
                                                                            aCTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTc                      <  1:670876/100‑1 (MQ=255)
                                                                             cTGGCCTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCg                     <  1:1425222/100‑1 (MQ=255)
                                                                               ggCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACACGTTATCTTTCTGCCGCCGCa                   >  2:1360171/1‑100 (MQ=255)
                                                                                gCCTTGCTGTCGTCAGGTAATGTCCCTACAAATGTTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCAGCCGTCGCaa                  >  2:2719929/1‑100 (MQ=255)
                                                                                gCCTTGCTGTCGTCAGGCCATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGc                          >  2:1972108/1‑92 (MQ=255)
                                                                                 ccTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCaaa                 >  2:1802093/1‑100 (MQ=255)
                                                                                 ccTTGCTGTCGTCAGGTAATGTACCTACAAATATTCCCACATTTGTGATAGCTCTCACCTTTTAACGTTGTCTGACAAGTTATCTTTCTGCCGTAGCaaa                 >  2:773596/1‑100 (MQ=255)
                                                                                 ccTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTGAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCaaa                 <  2:3279505/100‑1 (MQ=255)
                                                                                  cTTGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAAt                <  1:1794977/100‑1 (MQ=255)
                                                                                   ttGCTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATc               <  2:3374250/100‑1 (MQ=255)
                                                                                    tGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCa              >  2:3060057/1‑100 (MQ=255)
                                                                                      cTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATa            <  2:3012976/100‑1 (MQ=255)
                                                                                      cTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATa            >  2:2303057/1‑100 (MQ=255)
                                                                                      cTGTCGTCAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATa            <  2:1419036/100‑1 (MQ=255)
                                                                                        gtcgtcAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAg          <  2:901495/100‑1 (MQ=255)
                                                                                        gtcgtcAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAg          <  2:794092/100‑1 (MQ=255)
                                                                                        ggcgtcAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAg          <  1:2555524/98‑1 (MQ=255)
                                                                                          cgtcAGGCAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTc        <  2:2662321/100‑1 (MQ=255)
                                                                                           gtcAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCg       <  2:1671679/100‑1 (MQ=255)
                                                                                           gtcAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCg       >  1:3466093/1‑100 (MQ=255)
                                                                                              aGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCCCACCTTTTAAAGCTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACg    >  2:3556538/1‑100 (MQ=255)
                                                                                               ggTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACgg   <  2:1276618/100‑1 (MQ=255)
                                                                                                gTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGa  >  2:2847454/1‑100 (MQ=255)
                                                                                                 |                                                                                                  
TTCGTGAGTTAGATCAATAAACGTAGTTAAAAAAATTACTCTCAAAGTGGTAAATCTCGCTGCAGGCCGCGCCAGTACTGGCCTTGCTGTCGTCAGGTAATGTCCCTACAAATATTCCCACATTTGTGATGGCTCTCACCTTTTAAAGTTGTATGACAAGTTATCTTTCTGCCGTCGCAAATCATAAGTCGACGGA  >  NC_000913/3244831‑3245026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: