Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,417,572 C→T 15.9% A847V (GCG→GTG)  acrF → multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,417,5720CT15.9% 470.2 / 66.9 196A847V (GCG→GTG) acrFmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (83/81);  new base T (18/13);  total (102/94)
Fisher's exact test for biased strand distribution p-value = 5.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

Only 100 of 201 total aligned reads displayed.

GACTGGAACGCTACAACGGTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGCTCTGGTAGCAATTTCCTT  >  NC_000913/3417473‑3417671
                                                                                                   |                                                                                                   
gACTGGAACGCTACAACGGTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGc                                                                                                     <  1:346782/100‑1 (MQ=255)
 aCTGGAACGCTACAACGCTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCg                                                                                                    <  2:3394729/100‑1 (MQ=255)
  cTGGAACGCTACAACGGTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGTCATGGCGTTGATGGAAAACCTTGCGt                                                                                                   >  1:1511260/1‑100 (MQ=255)
  cTGGAACGCTACAACGGTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGt                                                                                                   >  2:2681357/1‑100 (MQ=255)
   tGGAACGCTACAACGGTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGTGTc                                                                                                  <  1:616860/100‑1 (MQ=255)
   tGGAACGCTACAACGGTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCAATGGCGTTGATGGAAAACCTTGCGTc                                                                                                  >  1:1789798/1‑100 (MQ=255)
    ggAACGCTACAACGGTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCa                                                                                                 >  2:2455422/1‑100 (MQ=255)
    ggAACGCTACAACGGTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCa                                                                                                 >  1:2974990/1‑100 (MQ=255)
     gAACGCTACAACGGTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCaa                                                                                                >  1:936324/1‑100 (MQ=255)
       aCGCTACAACGGTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCaaa                                                                                               >  1:1253777/1‑99 (MQ=255)
         gCTACAACGGTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAAtt                                                                                            >  1:2012530/1‑100 (MQ=255)
          cTACAACGGTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTGCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTa                                                                                           >  2:2650790/1‑100 (MQ=255)
          cTACAACGGTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTa                                                                                           <  1:1892615/100‑1 (MQ=255)
            aCAACGGTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTAcc                                                                                         <  2:567552/100‑1 (MQ=255)
               aCGGTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGc                                                                                      >  2:2059016/1‑100 (MQ=255)
                cGGTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCg                                                                                     <  1:2355617/100‑1 (MQ=255)
                  gTCTGTCGTCAATGGAGATTCAGGGGGAAGGCGCGTCAGGAACCAGTTCTGGCGATGCGATGGCGTTGATGGAAAACCTTGGGTGAAAATTACGTGgggg                                                                                   >  2:3933152/1‑100 (MQ=255)
                   tCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGc                                                                                  <  1:696546/100‑1 (MQ=255)
                   tCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGc                                                                                  >  2:3026841/1‑100 (MQ=255)
                    ctcccGTCAATGGACAGTCAGGCGGAAGTCGCGTCAGCAATCATTTCCGGCTATGCCATGGGGGTGATGGAAAACCTTGCTCCAAAATTTCCTGCGGGCa                                                                                 <  2:1848209/97‑1 (MQ=255)
                     tGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCAt                                                                                >  1:3025890/1‑100 (MQ=255)
                     tGCCGTCAATGCAGATTCAGGGTGACGCCGCGCCATGAACCAGTTCCGGCGATCCCATGGGGTTGATGGAAAACCTTGCGTCACATTTTCCTGCggg                                                                                   <  2:2550019/97‑1 (MQ=255)
                      gCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCAtt                                                                               >  2:3733283/1‑100 (MQ=255)
                        cGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTgg                                                                             >  2:2828195/1‑100 (MQ=255)
                         gTCCATGTAGATTTAGGGGGAAGGCGCGCTAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTgg                                                                             <  2:325056/99‑1 (MQ=255)
                           cAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGtt                                                                           <  2:2300931/99‑1 (MQ=255)
                             aTGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGTGTCAAAATTACCTGCGGGCATTGGTTATg                                                                        <  1:869965/100‑1 (MQ=255)
                             aTGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGTGTCAAAATTACCTGCGGGCATTGGTTATg                                                                        >  1:1014298/1‑100 (MQ=255)
                             aTGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATg                                                                        <  2:3172026/100‑1 (MQ=255)
                             aTGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATg                                                                        >  1:517641/1‑100 (MQ=255)
                             aTGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATg                                                                        <  2:1290360/100‑1 (MQ=255)
                              tGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGa                                                                       <  2:1224462/100‑1 (MQ=255)
                               ggAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGTGTCAAAATTACCTGCGGGCATTGGTTATGAc                                                                      <  2:49001/100‑1 (MQ=255)
                                gagaTTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACt                                                                     <  1:3535423/100‑1 (MQ=255)
                                gagaTTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACt                                                                     >  1:3387506/1‑100 (MQ=255)
                                 agaTTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGAc                                                                      <  1:1590399/98‑1 (MQ=255)
                                  gaTTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTgg                                                                   >  1:1554899/1‑100 (MQ=255)
                                    ttCCGGGGGCAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAGTTACCTGCGGGGGTTGGTTATGACTGGa                                                                  >  2:667258/1‑99 (MQ=255)
                                     tCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACg                                                                >  1:4028375/1‑100 (MQ=255)
                                      naGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACgg                                                               >  2:633490/2‑100 (MQ=255)
                                       aGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACggg                                                              <  2:1476810/100‑1 (MQ=255)
                                       aGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACggg                                                              <  1:4021483/100‑1 (MQ=255)
                                        gggggAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGCCg                                                                >  2:1410635/1‑97 (MQ=255)
                                        gggggAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGt                                                             >  2:383710/1‑100 (MQ=255)
                                         ggggAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTa                                                            <  2:886126/100‑1 (MQ=255)
                                         ggggAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTa                                                            <  1:3982894/100‑1 (MQ=255)
                                         ggggAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTa                                                            >  1:1208696/1‑100 (MQ=255)
                                             aaGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTc                                                        >  1:285783/1‑100 (MQ=255)
                                             aaGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTc                                                        >  1:3857495/1‑100 (MQ=255)
                                              aGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCg                                                       <  1:2138902/100‑1 (MQ=255)
                                              aGCCGCGCCAGGAAACAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGTGTCAAAATTACCTGCGGGCATTGGTTATGAATGAACAGAAATGTCg                                                       >  2:2656983/1‑100 (MQ=255)
                                                 cgcgCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTAt                                                    <  1:3560685/100‑1 (MQ=255)
                                                    gcCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAg                                                 >  2:3158950/1‑100 (MQ=255)
                                                    gcCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAg                                                 >  1:2545660/1‑100 (MQ=255)
                                                     ccAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAgg                                                >  2:3575164/1‑100 (MQ=255)
                                                      cAGGAACCATTTCCGGCGATGCCATGGCGTTTATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATc                                                   <  2:403450/96‑1 (MQ=255)
                                                      cAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGa                                               >  1:3030566/1‑100 (MQ=255)
                                                        ggAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGTGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAAc                                             <  2:596660/100‑1 (MQ=255)
                                                         gAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACg                                            <  2:3536234/100‑1 (MQ=255)
                                                            ccAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCtt                                         >  1:3817645/1‑100 (MQ=255)
                                                             cAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTa                                        <  1:2670385/100‑1 (MQ=255)
                                                               gTTCCGGCGATGCCATGGCGTTGATGGAAAAGCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATc                                      >  2:3681571/1‑100 (MQ=255)
                                                               gTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGTGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATc                                      >  1:2617828/1‑100 (MQ=255)
                                                               gTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATc                                      >  2:2153706/1‑100 (MQ=255)
                                                               gTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATc                                      >  1:2347769/1‑100 (MQ=255)
                                                                ttCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCg                                     <  1:1310464/100‑1 (MQ=255)
                                                                 tCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCgg                                    >  2:2920060/1‑100 (MQ=255)
                                                                 tCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCgg                                    <  2:1508578/100‑1 (MQ=255)
                                                                  ccGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCggg                                   <  1:413124/100‑1 (MQ=255)
                                                                  ccGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCggg                                   >  2:1614263/1‑100 (MQ=255)
                                                                    ggCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCggg                                   >  2:4065433/1‑98 (MQ=255)
                                                                    ggCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGaa                                 <  1:3960777/100‑1 (MQ=255)
                                                                      cGATGCCATGGCGTGGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAAc                               <  2:3950584/100‑1 (MQ=255)
                                                                       gATGCCATGGCGTTGATGGAAAACCTTGTGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAAc                               <  2:1014292/99‑1 (MQ=255)
                                                                       gATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAAcc                              <  1:3402337/100‑1 (MQ=255)
                                                                          ggcATGGGGGTGGTGGAAAGCCTTGTGGCGAAATGACCTGCGGGCATTGGTTATGACGGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAgg                           <  2:4023793/98‑1 (MQ=255)
                                                                          gCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAgg                           >  2:2263184/1‑100 (MQ=255)
                                                                          gCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAgg                           <  2:210132/100‑1 (MQ=255)
                                                                           ccATGGCGTTGATGGAAAACCTTGTGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGc                          >  2:1663900/1‑100 (MQ=255)
                                                                           ccATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGc                          <  1:2323008/100‑1 (MQ=255)
                                                                              tGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTcc                       <  1:1262234/100‑1 (MQ=255)
                                                                                 cGTTGATGGAAAACCTTGTGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGc                    >  1:2868835/1‑100 (MQ=255)
                                                                                 cGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGc                    >  1:3901778/1‑100 (MQ=255)
                                                                                  gTTGATGGAAAACCTTGTGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGc                    >  1:1764149/1‑99 (MQ=255)
                                                                                    tGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGctct                 >  2:2355333/1‑100 (MQ=255)
                                                                                     gATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGCTCTg                >  1:3732503/1‑100 (MQ=255)
                                                                                     gATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGACGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGCTTTg                >  1:2854575/1‑100 (MQ=255)
                                                                                      aTGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTGTGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGCTCTgg               <  1:2379105/100‑1 (MQ=255)
                                                                                      aTGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGACCGCTTATCGGGAAACCAGGCTCCCGCTCTgg               >  2:726595/1‑100 (MQ=255)
                                                                                      aTGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGCTCTgg               >  1:3396738/1‑100 (MQ=255)
                                                                                       tGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGCTCTGGt              <  1:283999/100‑1 (MQ=255)
                                                                                          aaaaCCTTGCGTCAAAATTACCTGCAGGCATTGGTTATAACTGGACGCGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGCTCTGGTAg            <  1:3933178/99‑1 (MQ=255)
                                                                                           aaaCCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGCTCTGGTAGCa          >  1:744081/1‑100 (MQ=255)
                                                                                             aCCTTGCGTCAAAATTGCCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGCTCTGGTAGCAAt        <  2:142639/100‑1 (MQ=255)
                                                                                               cTTGTGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCGCCCGCTCTGGTAGCAAttt      <  2:1655748/100‑1 (MQ=255)
                                                                                               cTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGCTCTGGTAg            <  2:1573031/94‑1 (MQ=255)
                                                                                               cTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGCTCTGGTAGCAAttt      >  1:3660178/1‑100 (MQ=255)
                                                                                               cTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGCTCTGGTAGCAAttt      <  1:3747156/100‑1 (MQ=255)
                                                                                                   cGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGCTCTGGTAGCAATTTCCtt  <  1:3158969/100‑1 (MQ=255)
                                                                                                   cGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCACGCTCTGGTAGCAATTTCCt   >  2:1973223/1‑99 (MQ=255)
                                                                                                   |                                                                                                   
GACTGGAACGCTACAACGGTCTGCCGTCAATGGAGATTCAGGGGGAAGCCGCGCCAGGAACCAGTTCCGGCGATGCCATGGCGTTGATGGAAAACCTTGCGTCAAAATTACCTGCGGGCATTGGTTATGACTGGACGGGTATGTCGTATCAGGAACGCTTATCGGGAAACCAGGCTCCCGCTCTGGTAGCAATTTCCTT  >  NC_000913/3417473‑3417671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: