Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,452,057 A→T 100% I77N (ATC→AAC)  rplD ← 50S ribosomal subunit protein L4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,452,0570AT98.7% 611.5 / ‑2.5 188I77N (ATC→AAC) rplD50S ribosomal subunit protein L4
Reads supporting (aligned to +/- strand):  ref base A (3/0);  new base T (77/106);  total (82/106)
Fisher's exact test for biased strand distribution p-value = 7.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

CAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGC  >  NC_000913/3451960‑3452154
                                                                                                 |                                                                                                 
cAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTc                                                                                                 <  1:1974586/100‑1 (MQ=255)
 aGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCg                                                                                                <  1:3699495/100‑1 (MQ=255)
  ggATGCTTTTCGGCGCGCCGCGGTACATCTACGTGTTGACTTTTTGACTGGGGGCCTGCGGACGAGCAGCAAAAGTCACGCCACCAGAACGCCAGTTCgg                                                                                               <  2:425560/100‑1 (MQ=255)
  ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCgg                                                                                               >  1:390611/1‑100 (MQ=255)
  ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCgg                                                                                               >  1:4034827/1‑100 (MQ=255)
  ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCgg                                                                                               >  1:4122860/1‑100 (MQ=255)
   gATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCggg                                                                                              <  2:3042255/100‑1 (MQ=255)
    aTGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGc                                                                                             <  2:3757977/100‑1 (MQ=255)
      gatttcCAGCGCGCCGGGGTACAGCGTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCATCCAGAACGCCAGTTCggg                                                                                              <  2:3191716/95‑1 (MQ=255)
      gCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGctc                                                                                           >  2:1095543/1‑100 (MQ=255)
       cTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGctct                                                                                          >  1:1556234/1‑100 (MQ=255)
        ttttCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCtt                                                                                         <  2:2798330/100‑1 (MQ=255)
         tttCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTg                                                                                        <  2:2192398/100‑1 (MQ=255)
         nttCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTg                                                                                        >  2:1671544/2‑100 (MQ=255)
           tCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGAt                                                                                      <  1:544789/100‑1 (MQ=255)
           tCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGAt                                                                                      <  1:2135003/100‑1 (MQ=255)
            cAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGATCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATa                                                                                     <  1:2554480/100‑1 (MQ=255)
             aGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAg                                                                                    >  2:3153090/1‑100 (MQ=255)
             aGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAg                                                                                    <  1:1379183/100‑1 (MQ=255)
              gcgcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGa                                                                                   <  1:3753225/100‑1 (MQ=255)
                  gccgcGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCA                                                                               <  1:1109138‑M1/100‑1 (MQ=255)
                        gTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAAcgc                                                                         >  1:2227554/1‑100 (MQ=255)
                         tACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAAcgcg                                                                        <  2:74887/100‑1 (MQ=255)
                           cATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCa                                                                      <  2:160192/100‑1 (MQ=255)
                             tcttctTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACg                                                                    >  2:1775925/1‑100 (MQ=255)
                              cttcttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGCACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACgg                                                                   <  2:3973578/100‑1 (MQ=255)
                              cgtcttGTGAACTTTTGTACTTTGCTCCTGCGGCCGCGCAGCCAAGGTCCCGCCACCAGAACGCCAGTTCGGGCTCTTGTTAGAACCAGATCgcgc                                                                       <  2:2042515/94‑1 (MQ=255)
                                tcttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGcc                                                                 >  1:1761177/1‑100 (MQ=255)
                                   tGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGt                                                              >  1:3474562/1‑100 (MQ=255)
                                    gTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTg                                                             <  1:636800/100‑1 (MQ=255)
                                      tAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGc                                                            <  1:528805/99‑1 (MQ=255)
                                       aaCTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCt                                                          >  2:3488476/1‑100 (MQ=255)
                                         cTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTtt                                                        <  2:133845‑M2/100‑3 (MQ=255)
                                         cTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTtt                                                        <  1:3612531‑M2/100‑3 (MQ=255)
                                         cTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTtt                                                        <  1:3743012‑M2/100‑3 (MQ=255)
                                          tttttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTttc                                                       >  2:3284204‑M2/1‑97 (MQ=255)
                                          tttttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTttc                                                       <  1:1543604‑M2/100‑4 (MQ=255)
                                            tttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTttctg                                                     <  1:2669648‑M2/100‑6 (MQ=255)
                                              tGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAAaggcaccggccgtgcgcgttctggt                                                   <  2:2303031‑M1/100‑26 (MQ=255)
                                              tGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAAaggcaccggccgtgcgcgttctggn                                                   <  1:2057343‑M1/100‑26 (MQ=255)
                                               gACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGgcg                                                  <  1:3020337/100‑1 (MQ=255)
                                                aCTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGCTAGAACCAGAAaggcaccggccgtgcgcattccggttc                                                 <  2:186431‑M1/100‑28 (MQ=255)
                                                aCTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGgcgc                                                 <  2:4134452/100‑1 (MQ=255)
                                                 cTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGcc                                                >  1:2186334/1‑100 (MQ=255)
                                                 cTGGGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGcc                                                <  2:1808306/100‑1 (MQ=255)
                                                   gtgGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAAaggcaccggccgtgcgcgttctggttctat                                              <  1:892137‑M1/100‑31 (MQ=255)
                                                    tgGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACg                                             >  1:956788/1‑100 (MQ=255)
                                                     ggTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACgg                                            <  1:736302/100‑1 (MQ=255)
                                                     ggTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACgg                                            >  1:3295476/1‑100 (MQ=255)
                                                      gTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGt                                           >  2:2441489/1‑100 (MQ=255)
                                                       tCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtt                                          >  1:1639820/1‑100 (MQ=255)
                                                        ccTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGttt                                         <  2:428067/100‑1 (MQ=255)
                                                        ccTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGttt                                         >  1:2107061/1‑100 (MQ=255)
                                                        ccTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGAGCACCGCCGGTTCCTTTCTGGCACCACGGttt                                         >  2:1451849/1‑100 (MQ=255)
                                                         cTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttt                                        <  2:2768959/100‑1 (MQ=255)
                                                           gCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttttt                                      >  2:3827309/1‑100 (MQ=255)
                                                            cGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTa                                     <  1:4109570/100‑1 (MQ=255)
                                                                cGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCgg                                 >  2:2120872/1‑100 (MQ=255)
                                                                  agcagcAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGaa                               <  2:3468653/100‑1 (MQ=255)
                                                                    cagcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAcc                             <  2:3224075/100‑1 (MQ=255)
                                                                     agcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCa                            >  2:3310286/1‑100 (MQ=255)
                                                                     accaAAGGTCACGCCACTAGAACGCCAGCTCAGCCTCATCATAACCCCAGACCGCGCACGGCCGATGCCCTCCTGCCGCCACGGTTCGTTACCgtaccta                            >  2:1568681/3‑94 (MQ=255)
                                                                      gcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAg                           >  1:133111/1‑100 (MQ=255)
                                                                      gcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAg                           <  2:560992/100‑1 (MQ=255)
                                                                      gcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAg                           <  2:4034801/100‑1 (MQ=255)
                                                                      gcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAg                           >  1:3384889/1‑100 (MQ=255)
                                                                      gcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTGGATAGAACCAGAACGCGCCCGGCCGGTACCTTTATGACGCCACGGTTTTTTACCGGAACCa                            >  2:985568/1‑99 (MQ=255)
                                                                       caAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGt                          <  2:2455033/100‑1 (MQ=255)
                                                                        aaaGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGtt                         <  1:1811636/100‑1 (MQ=255)
                                                                        aaaGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGtt                         >  1:3310881/1‑100 (MQ=255)
                                                                          aGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTAc                       <  1:2427102/100‑1 (MQ=255)
                                                                          aGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTAc                       <  1:810496/100‑1 (MQ=255)
                                                                          aGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTAc                       <  1:468196/100‑1 (MQ=255)
                                                                          aGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACAGGAACCAGTTa                        <  2:3350248/99‑1 (MQ=255)
                                                                           ggTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACt                      <  2:1191299/100‑1 (MQ=255)
                                                                           ggTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACt                      <  1:3528187/100‑1 (MQ=255)
                                                                             tCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCTGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTc                    <  2:3052130/100‑1 (MQ=255)
                                                                             tCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACttn                    <  2:2988484/100‑2 (MQ=255)
                                                                             tCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTc                    >  1:1052817/1‑100 (MQ=255)
                                                                              cACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCa                   <  1:1790092/100‑1 (MQ=255)
                                                                              cACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGCTTTTTACCGGCACCAGTTACTTCa                   >  1:2697145/1‑100 (MQ=255)
                                                                              cACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGATTTTTACCGGAACCAGTTACTTCa                   >  2:810949/1‑100 (MQ=255)
                                                                                cGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCGTTCTGGCGCCGCGGTTTTTTACCGGAACCAGTTACTTCAGc                 >  2:518146/1‑100 (MQ=255)
                                                                                 gCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCa                <  1:1934016/100‑1 (MQ=255)
                                                                                 gCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCa                <  2:1253712/100‑1 (MQ=255)
                                                                                  ccaccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCa                <  2:663098/99‑1 (MQ=255)
                                                                                  ccaccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCAc               >  1:3161786/1‑100 (MQ=255)
                                                                                   caccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACg              <  1:2398572/100‑1 (MQ=255)
                                                                                     ccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAg            <  1:932365/100‑1 (MQ=255)
                                                                                     ccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAg            <  1:1762574/100‑1 (MQ=255)
                                                                                     ccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAg            <  1:1510902/100‑1 (MQ=255)
                                                                                      caGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGt           >  2:3361870/1‑100 (MQ=255)
                                                                                       aGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtc          >  2:2879663/1‑100 (MQ=255)
                                                                                       aGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtc          >  1:3413417/1‑100 (MQ=255)
                                                                                        gAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtct         >  1:2322447/1‑100 (MQ=255)
                                                                                        gAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtct         >  2:3271127/1‑100 (MQ=255)
                                                                                          aCGCCAGCTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtcttc       >  1:2163903/1‑100 (MQ=255)
                                                                                           cGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtcttct      >  2:2573103/1‑100 (MQ=255)
                                                                                            gCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTg     <  2:1965566/100‑1 (MQ=255)
                                                                                             ccAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGa    >  1:2039193/1‑100 (MQ=255)
                                                                                                 |                                                                                                 
CAGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGC  >  NC_000913/3451960‑3452154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: