Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,469,544 G→C 49.9% P104A (CCT→GCT)  chiA ← periplasmic endochitinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,469,5440GC49.9% 3.7 / inf 217P104A (CCT→GCT) chiAperiplasmic endochitinase
Reads supporting (aligned to +/- strand):  ref base G (62/46);  new base C (54/54);  total (117/100)
Fisher's exact test for biased strand distribution p-value = 3.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 223 total aligned reads displayed.

CCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACG  >  NC_000913/3469447‑3469643
                                                                                                 |                                                                                                   
    gctgTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTatt                                                                                               <  1:2387519/100‑1 (MQ=255)
     ctttttATTCCAGGAAACAAAAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAACCGAACCGCCATTAGACAGCGTATTGGAAGCAGCCGTatt                                                                                               <  2:185103/96‑1 (MQ=255)
      tgTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttg                                                                                             >  2:2380458/1‑100 (MQ=255)
      tgTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttg                                                                                             <  1:949694/100‑1 (MQ=255)
       gTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTatttga                                                                                            >  1:1405273/1‑100 (MQ=255)
         ttATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttgaat                                                                                          >  2:45303/1‑100 (MQ=255)
         ttATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTatttgaat                                                                                          <  1:183698/100‑1 (MQ=255)
           aTTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttgaattt                                                                                        >  2:3466474/1‑100 (MQ=255)
             tCCATGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttgaatttga                                                                                      <  2:3551721/100‑1 (MQ=255)
             tCCAGGCAACTACAGAAGAATTAGACTGCACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTACTGGATGCAGCCGTattcgaatttga                                                                                      >  2:1581183/1‑100 (MQ=255)
             tCCAGGCAACTACAGAAGAATTAGACGTAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTatttgaatttga                                                                                      <  2:1657469/100‑1 (MQ=255)
             tCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTatttgaatttga                                                                                      >  1:846755/1‑100 (MQ=255)
              ccAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGGAtttgaatttgaa                                                                                     >  2:197660/1‑100 (MQ=255)
              ccAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTAtttgaatttgaa                                                                                     <  1:1763999/100‑1 (MQ=255)
              ccAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTAtttgaatttgaa                                                                                     <  1:1293005/100‑1 (MQ=255)
               ctggCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTgaag                                                                                    >  1:2828179/3‑100 (MQ=255)
               cAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTgaag                                                                                    <  1:1068476/100‑1 (MQ=255)
               cAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTgaag                                                                                    >  1:1772425/1‑100 (MQ=255)
               cAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTgaag                                                                                    >  2:191293/1‑100 (MQ=255)
               cAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTgaag                                                                                    >  2:3427965/1‑100 (MQ=255)
                aGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTgaaga                                                                                   <  2:3194747/100‑1 (MQ=255)
                 ggCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTgaagaa                                                                                  >  1:1704889/1‑100 (MQ=255)
                 ggCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTgaagaa                                                                                  >  1:264669/1‑100 (MQ=255)
                 ggCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTgaagaa                                                                                  >  1:2505688/1‑100 (MQ=255)
                    aaCTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAagaagag                                                                               >  2:2954571/1‑99 (MQ=255)
                       tACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAg                                                                            <  2:2452375/100‑1 (MQ=255)
                        aCAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGc                                                                           >  1:3194889/1‑100 (MQ=255)
                          agaagaATAAGACGGAACTGTGCCCTGTGCTGGTGTAGCCCAACCTCCATACGCCCGCGTCTTCGATCCAGCCTTCCTCGAAGTTGAAGAAGATCAGCTg                                                                         >  2:67665/1‑100 (MQ=255)
                           gaagaaTTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGc                                                                        >  1:2098308/1‑100 (MQ=255)
                            nagaaTTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGcc                                                                       >  1:3787940/2‑100 (MQ=255)
                            aagaaTTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGcc                                                                       <  1:1692475/100‑1 (MQ=255)
                            aagaaTTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGcc                                                                       <  2:1259951/100‑1 (MQ=255)
                             agaaTTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCg                                                                      >  1:3558723/1‑100 (MQ=255)
                                aTTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGctc                                                                   >  1:3725251/1‑100 (MQ=255)
                                   aGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCttt                                                                >  1:2051634/1‑100 (MQ=255)
                                      cGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCg                                                             >  2:482756/1‑100 (MQ=255)
                                       ggAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGc                                                            <  1:3424537/100‑1 (MQ=255)
                                       ggAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGc                                                            <  2:2880273/100‑1 (MQ=255)
                                        gAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCa                                                           <  2:4049956/100‑1 (MQ=255)
                                         aaCAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAg                                                          >  2:3837774/1‑100 (MQ=255)
                                          aCAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAgg                                                         <  2:1772413/100‑1 (MQ=255)
                                          aCAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAgg                                                         >  2:2727688/1‑100 (MQ=255)
                                           cAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGa                                                        <  2:1360486/100‑1 (MQ=255)
                                             gTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGaaa                                                      >  1:2729837/1‑100 (MQ=255)
                                               gCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAgt                                                    >  2:256030/1‑100 (MQ=255)
                                                cccTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAgtg                                                   <  1:437087/100‑1 (MQ=255)
                                                  cTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTa                                                 <  1:2194623/100‑1 (MQ=255)
                                                   tgtgCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTAt                                                >  2:3299624/1‑100 (MQ=255)
                                                    gtgCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTAtt                                               >  1:3382237/1‑100 (MQ=255)
                                                     tgCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTg                                              <  1:599647/100‑1 (MQ=255)
                                                          gtgtAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCaa                                          >  2:3313433/1‑99 (MQ=255)
                                                          gtgtAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCaaa                                         >  2:151635/1‑100 (MQ=255)
                                                             tAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTg                                      <  1:491137/100‑1 (MQ=255)
                                                             tAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTg                                      <  1:1375323/100‑1 (MQ=255)
                                                               gCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGAc                                    >  1:261238/1‑100 (MQ=255)
                                                               gCCGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGAc                                    <  1:252135/100‑1 (MQ=255)
                                                                ccGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACt                                   >  1:1613755/1‑100 (MQ=255)
                                                                ccGAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACt                                   >  2:962546/1‑100 (MQ=255)
                                                                  gAACCGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTaa                                 >  1:1547087/1‑100 (MQ=255)
                                                                     ccGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAAttt                              >  1:2174451/1‑100 (MQ=255)
                                                                      cGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTc                             <  2:1093822/100‑1 (MQ=255)
                                                                      cGCCATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTc                             <  2:3266649/100‑1 (MQ=255)
                                                                       gCCATTAGCCGGCGGCTGGGCTGCAGCCGTATTTGAATTTGACGAAGATGACCTGCAGCTCTTTTCGGAGGAAAGTGTATCGCCAAACTGACTATTTTca                            >  2:1318843/1‑100 (MQ=255)
                                                                        ccATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTcag                           >  2:3734512/1‑100 (MQ=255)
                                                                        ccATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTcag                           <  1:463083/100‑1 (MQ=255)
                                                                         cATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTcagc                          <  2:1254058/100‑1 (MQ=255)
                                                                         cATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTcagc                          >  1:1009248/1‑100 (MQ=255)
                                                                         cATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTcagc                          <  2:1681580/100‑1 (MQ=255)
                                                                         cATTAGCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTcagc                          <  1:2013050/100‑1 (MQ=255)
                                                                           ttAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTcagcag                        <  1:2365612/100‑1 (MQ=255)
                                                                            tAGCCGGCGTATTGTATGCAGCCGTATTTGAATTTGAAGAAGATGATCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCagcagc                       <  2:769521/100‑1 (MQ=255)
                                                                             aGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCt                      >  1:3033226/1‑100 (MQ=255)
                                                                              gCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTg                     <  1:1108996/100‑1 (MQ=255)
                                                                              gCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTg                     >  1:1239222/1‑100 (MQ=255)
                                                                              gCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTg                     <  1:886575/100‑1 (MQ=255)
                                                                              gCCGGCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTg                     >  2:1722358/1‑100 (MQ=255)
                                                                               ccGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGt                    >  1:2336908/1‑100 (MQ=255)
                                                                                 ggCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTg                  >  1:1919918/1‑100 (MQ=255)
                                                                                  ttccgatctaTGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGc                 <  1:4090111/91‑1 (MQ=255)
                                                                                  gCGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGc                 <  2:3212666/100‑1 (MQ=255)
                                                                                   cGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCg                >  2:804941/1‑100 (MQ=255)
                                                                                   cGTATTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCg                <  2:3767132/100‑1 (MQ=255)
                                                                                      aTTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCgg               >  2:3574726/1‑98 (MQ=255)
                                                                                      aTTGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTa             <  1:582919/100‑1 (MQ=255)
                                                                                       ttGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTAc            <  1:535433/100‑1 (MQ=255)
                                                                                       ttGGATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTAc            <  2:4083152/100‑1 (MQ=255)
                                                                                         ggATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGc          <  1:3290248/100‑1 (MQ=255)
                                                                                         ggATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGc          <  2:3545665/100‑1 (MQ=255)
                                                                                         ggATGCAGCCGTATTTGAATGTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGc          >  2:2532839/1‑100 (MQ=255)
                                                                                          gATGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCt         >  1:2516039/1‑100 (MQ=255)
                                                                                            tGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCttt       >  1:773998/1‑100 (MQ=255)
                                                                                            tGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCttt       >  2:3628277/1‑100 (MQ=255)
                                                                                            tGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCttt       <  2:2185706/100‑1 (MQ=255)
                                                                                            tGCAGCCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCttt       >  2:1828063/1‑100 (MQ=255)
                                                                                                ggCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAAc   <  1:2692506/100‑1 (MQ=255)
                                                                                                 gCGTATTTGAATTTGAAGAAGATGCGCTGCCGCTCTTTTCGGAGGAAAGTGTATTGCCAAACTGACTAAGTTCAGCAGCTGTTGCGGTACGCTTTAAACg  <  2:3889505/100‑1 (MQ=255)
                                                                                                 gCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAAca  >  1:672448/1‑99 (MQ=255)
                                                                                                 gCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACg  <  1:383120/100‑1 (MQ=255)
                                                                                                 gCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACg  <  2:1753466/100‑1 (MQ=255)
                                                                                                 gCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACg  <  2:3033207/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
CCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACG  >  NC_000913/3469447‑3469643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: