Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,477,434 T→C 25.9% intergenic (‑15/‑206) fkpA ← / → slyX FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)/phi X174 lysis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,477,4340TC25.9% 279.2 / 117.3 182intergenic (‑15/‑206)fkpA/slyXFKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)/phi X174 lysis protein
Reads supporting (aligned to +/- strand):  ref base T (65/69);  new base C (26/21);  total (91/91)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.67e-01

Only 100 of 185 total aligned reads displayed.

CAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTATTTTC  >  NC_000913/3477335‑3477533
                                                                                                   |                                                                                                   
   cTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCgg                                                                                                  >  1:2730013/1‑100 (MQ=255)
    ttCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCggg                                                                                                 <  1:1904223/100‑1 (MQ=255)
    ttCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCggg                                                                                                 <  2:449390/100‑1 (MQ=255)
     tcggcagcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCgggg                                                                                                <  1:734727/97‑1 (MQ=255)
       aggagcaAAAGTGATTCGTGCATGAAGGGCAACGGCCATTTTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCgggg                                                                                                <  2:4001528/95‑1 (MQ=255)
       agcagcAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCggggag                                                                                              >  1:1461090/1‑98 (MQ=255)
        gcagcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCAt                                                                                             >  2:1492183/1‑100 (MQ=255)
        gcagcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAt                                                                                             >  2:3271652/1‑100 (MQ=255)
         cagcaAAAGTGATTGGTGCATGCAGGTCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtc                                                                                            <  2:436033/100‑1 (MQ=255)
         cagcaAAAGTGATTGGGGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtc                                                                                            <  2:1195252/100‑1 (MQ=255)
          agcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCAtct                                                                                           >  1:2290156/1‑100 (MQ=255)
           gcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtctc                                                                                          >  1:3728112/1‑100 (MQ=255)
                aGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCAcccc                                                                                     <  2:4092163/100‑1 (MQ=255)
                  tGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAg                                                                                   >  1:296675/1‑100 (MQ=255)
                   gATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAgg                                                                                  <  2:3777717/100‑1 (MQ=255)
                   gATTGGTGCATGCAGGGCAACGGCCATTGTAGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATGTCACCCCAgg                                                                                  >  2:2724118/1‑100 (MQ=255)
                    aTTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAggg                                                                                 <  2:2281168/100‑1 (MQ=255)
                    aTTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAgg                                                                                  <  2:2313129/99‑1 (MQ=255)
                     ttGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGt                                                                                <  1:3751261/100‑1 (MQ=255)
                       ggTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCGGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTGAACCCAGGGtt                                                                               >  2:256686/1‑99 (MQ=255)
                         tgcatgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAAc                                                                            >  1:2149414/1‑100 (MQ=255)
                         tgcatgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAAc                                                                            <  1:1615929/100‑1 (MQ=255)
                         tgcatgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAAc                                                                            <  2:533135/100‑1 (MQ=255)
                          gcatgcaGGGCAACGGCCATTGTGGTCGCCAGCAGTGTTACGCTAAACAGTGATGTCATCGATATCTCCAGTATCGGCGCCTCTCACCCCAGTGTTa                                                                              >  2:2344798/1‑97 (MQ=255)
                          gcatgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACt                                                                           <  1:3150321/100‑1 (MQ=255)
                          gcatgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACt                                                                           <  1:3431473/100‑1 (MQ=255)
                          gcatgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACt                                                                           <  2:1607051/100‑1 (MQ=255)
                             tgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACtatt                                                                        <  2:765875/100‑1 (MQ=255)
                              gcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACtatta                                                                       <  2:204458/100‑1 (MQ=255)
                                aGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATc                                                                     >  2:2747878/1‑100 (MQ=255)
                                aGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATc                                                                     <  1:1215551/100‑1 (MQ=255)
                                aGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATc                                                                     <  2:1085495/100‑1 (MQ=255)
                                 gggCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCa                                                                    <  1:1523176/100‑1 (MQ=255)
                                   gCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGa                                                                  >  1:10068/1‑100 (MQ=255)
                                    cAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGaa                                                                 >  2:3129350/1‑100 (MQ=255)
                                    cAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGaa                                                                 >  1:2393873/1‑100 (MQ=255)
                                    aaaaggCCATTGTGGTCGCCAGCCGCGTTACTTTAAACAGTGATTGCATCCATGTCTCCAGGCTCGGGGCATCTCGCCCCTCGGTTAACTATTATCAGa                                                                  >  2:3382504/5‑99 (MQ=255)
                                       cGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAggg                                                              <  2:3822652/100‑1 (MQ=255)
                                        ggCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGt                                                             >  1:306919/1‑100 (MQ=255)
                                          ccATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTAc                                                           <  1:3861975/100‑1 (MQ=255)
                                           cATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACt                                                          <  2:751420/100‑1 (MQ=255)
                                            aTTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACta                                                         <  1:950740/100‑1 (MQ=255)
                                            aTTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACta                                                         <  1:1336731/100‑1 (MQ=255)
                                                tgGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATaaa                                                     <  2:390929/100‑1 (MQ=255)
                                                tgGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATaaa                                                     >  1:1292340/1‑100 (MQ=255)
                                                 ggTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAg                                                    >  1:1397446/1‑100 (MQ=255)
                                                   tCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCg                                                  >  2:849125/1‑100 (MQ=255)
                                                    cGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCg                                                  >  2:315029/1‑99 (MQ=255)
                                                         gcagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCg                                            <  1:1696404/100‑1 (MQ=255)
                                                          cagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGa                                           <  2:2591949/100‑1 (MQ=255)
                                                            gcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGGTCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAc                                         >  1:350711/1‑100 (MQ=255)
                                                            gcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAc                                         >  2:717755/1‑100 (MQ=255)
                                                            gcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAc                                         >  1:1882515/1‑100 (MQ=255)
                                                              gTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACaa                                       >  1:4056337/1‑100 (MQ=255)
                                                              gTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACaa                                       >  2:2447536/1‑100 (MQ=255)
                                                              gTTACTTTAAACAGTGATTTCATCCAGATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACaa                                       >  1:2521160/1‑100 (MQ=255)
                                                                 aCTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATc                                    <  1:2878470/100‑1 (MQ=255)
                                                                 aCTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATc                                    >  1:1009663/1‑100 (MQ=255)
                                                                 aCTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATc                                    <  1:3687227/100‑1 (MQ=255)
                                                                  cTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCt                                   >  1:1938722/1‑100 (MQ=255)
                                                                  cTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCt                                   >  1:3678489/1‑100 (MQ=255)
                                                                  cTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCt                                   >  1:3371605/1‑100 (MQ=255)
                                                                      aaaCAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacat                               >  2:1394765/1‑100 (MQ=255)
                                                                       aaCAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacata                              >  1:493371/1‑100 (MQ=255)
                                                                        ccAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacatac                             >  1:3331262/2‑100 (MQ=255)
                                                                        aCAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAATAAATCtacatac                             <  1:2724134/100‑1 (MQ=255)
                                                                         cAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCTCCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacataca                            <  2:2246315/100‑1 (MQ=255)
                                                                         cAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacataca                            >  2:1533543/1‑100 (MQ=255)
                                                                              aTTTCATCCATATCTCTAGGATCGGGGCATCTCACCTCAGGGTTCACTATTCGCAGAAGGGTACTGTAAGGCGGTGTGTAACACATCtgaatgc                             >  2:3528134/1‑88 (MQ=255)
                                                                               tttCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACg                      <  1:3948150/100‑1 (MQ=255)
                                                                               tttCATCCATATCTCCAGGACCGGGGCATCTCACCCGAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACg                      >  1:2916547/1‑100 (MQ=255)
                                                                               tttCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAAGGTACTATAAAGCGTTGTCGAACAAATCtacataagatcgga                      >  2:2903197/1‑92 (MQ=255)
                                                                                ttCATCCATATCTCCAGGATCGGTGCATCTCACCCCAGGGTTATCTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGaca                        <  1:3097059/97‑1 (MQ=255)
                                                                                 tCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGcc                    <  2:296672/100‑1 (MQ=255)
                                                                                 tCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGcc                    <  1:2556057/100‑1 (MQ=255)
                                                                                  cATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGccc                   <  1:1808827/100‑1 (MQ=255)
                                                                                  cATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGccc                   >  2:431414/1‑100 (MQ=255)
                                                                                   aTCCATATCTCCAGGATCGGAGCATCTCACCCCAGGTTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGccc                   <  2:1325367/99‑1 (MQ=255)
                                                                                      cATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtatt               >  2:168231/1‑100 (MQ=255)
                                                                                      cATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtatt               <  2:2995177/100‑1 (MQ=255)
                                                                                      cATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtatt               >  1:1233395/1‑100 (MQ=255)
                                                                                      cATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtatt               >  1:2930439/1‑100 (MQ=255)
                                                                                       atatCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtatta              <  2:2238551/100‑1 (MQ=255)
                                                                                          tctcCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatca           <  1:3161579/100‑1 (MQ=255)
                                                                                          tctcCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatca           >  1:646263/1‑100 (MQ=255)
                                                                                          tctcCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatca           <  1:3588804/100‑1 (MQ=255)
                                                                                           ctcCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcat          >  2:3370509/1‑100 (MQ=255)
                                                                                           ctcCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcat          >  2:4011908/1‑100 (MQ=255)
                                                                                           ctcCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcat          >  1:1003358/1‑100 (MQ=255)
                                                                                            tcCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcatc         <  2:3622217/100‑1 (MQ=255)
                                                                                            tcCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcatc         >  2:2621592/1‑100 (MQ=255)
                                                                                            tcCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcatc         >  1:1075271/1‑100 (MQ=255)
                                                                                            tcCAGGAGCGGGGCATCTTACACCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACAGACAGACACGCCCTATTatcatc         <  2:186989/100‑1 (MQ=255)
                                                                                             ccAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCt        >  1:2218632/1‑100 (MQ=255)
                                                                                              cAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCtn       <  2:3947856/100‑2 (MQ=255)
                                                                                               aGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGAAACGCCCTATTATCATCTa       >  2:3519427/1‑99 (MQ=255)
                                                                                                 gATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTAttt    >  1:2068989/1‑100 (MQ=255)
                                                                                                  aTCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTAtttt   <  2:2133858/100‑1 (MQ=255)
                                                                                                  aTCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTAtttt   >  1:2599918/1‑100 (MQ=255)
                                                                                                   tCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTATTTTc  <  1:2447550/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTATTTTC  >  NC_000913/3477335‑3477533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: