Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,524,413 A→G 29.9% I515V (ATT→GTT)  mrcA → penicillin‑binding protein 1a, murein transglycosylase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,524,4130AG29.9% 249.8 / 155.1 208I515V (ATT→GTT) mrcApenicillin‑binding protein 1a, murein transglycosylase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (70/74);  new base G (36/26);  total (108/100)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

Only 100 of 212 total aligned reads displayed.

CTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGC  >  NC_000913/3524314‑3524511
                                                                                                   |                                                                                                  
    cacgTATGTTGAACGATGTGCCAATTTCTCGCTCGGATGCAAGTGCCGGTTCTGACTGGCAGCCGACGAACTCACCACCGCAGTCTGCTGGTCCAAt                                                                                                   >  2:3498173/4‑97 (MQ=255)
    cAAGTATGTTGAACGATGTGCCAATGTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCAGAAGAACTCACCACCGCAGTATGCTGGTCCaa                                                                                                    <  2:2437674/96‑1 (MQ=255)
      aGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCt                                                                                              <  1:229230/100‑1 (MQ=255)
      aGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCt                                                                                              <  1:3086526/100‑1 (MQ=255)
       gTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCtt                                                                                             >  2:1997270/1‑100 (MQ=255)
       gTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCtt                                                                                             <  2:2697865/100‑1 (MQ=255)
         aTGTTGAACGATGTGCCAATTTCTCGCTGTGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCAGCGCAGTATGCTGGTCCAATTCGCtt                                                                                             <  2:1499519/98‑1 (MQ=255)
          tGTTGAACGATGTGCCAATTTCTCGCTGGGATTCAAGTGCCGGTTCTGACTGCCAGCCGAAGAACTCACCACCTCAGTATGCTGGTCCAATTCGCTTACg                                                                                          <  2:3488876/100‑1 (MQ=255)
          tGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACg                                                                                          >  2:1452542/1‑100 (MQ=255)
          tGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACg                                                                                          <  2:2569753/100‑1 (MQ=255)
           gTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGt                                                                                         >  1:3365764/1‑100 (MQ=255)
             tGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCa                                                                                       <  1:589775/100‑1 (MQ=255)
             tGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCa                                                                                       >  2:2086455/1‑100 (MQ=255)
               aaCGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAgg                                                                                     <  2:2059904/100‑1 (MQ=255)
               aaCGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAgg                                                                                     <  1:1645480/100‑1 (MQ=255)
                 cGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAgggg                                                                                   <  1:1972436/100‑1 (MQ=255)
                    tgtgCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTg                                                                                <  1:2742476/100‑1 (MQ=255)
                     gtgCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTgg                                                                               <  1:1654023/100‑1 (MQ=255)
                     gtgCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAggg                                                                                    >  2:3274427/1‑95 (MQ=255)
                      tgCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTggg                                                                              <  1:34713/100‑1 (MQ=255)
                      tgCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTggg                                                                              <  2:2230991/100‑1 (MQ=255)
                       gCCAATTACTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTgg                                                                               >  2:2167911/1‑98 (MQ=255)
                        ccAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTc                                                                            >  1:1134049/1‑100 (MQ=255)
                         cAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTc                                                                            <  1:1466948/99‑1 (MQ=255)
                         cAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCa                                                                           >  1:1425448/1‑100 (MQ=255)
                         cAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCa                                                                           >  2:112288/1‑100 (MQ=255)
                          aaTTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAg                                                                          <  2:4049130/100‑1 (MQ=255)
                          aaTTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAg                                                                          <  2:3070574/100‑1 (MQ=255)
                             ttCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCg                                                                       <  2:1624924/100‑1 (MQ=255)
                              tctcGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTc                                                                        <  2:3698541/98‑1 (MQ=255)
                                 cGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGaaaa                                                                   <  1:1314834/100‑1 (MQ=255)
                                   cTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAAc                                                                 >  1:2884378/1‑100 (MQ=255)
                                     gggATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgt                                                               >  2:1391602/1‑100 (MQ=255)
                                      ggATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtg                                                              <  1:1918614/100‑1 (MQ=255)
                                       gATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCACTTCGCTTACGTCAGGGGCTGGGTCAGGCGAAACACgtgg                                                             >  2:1334517/1‑100 (MQ=255)
                                       gATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtgg                                                             <  2:2342737/100‑1 (MQ=255)
                                       gATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtgg                                                             >  1:2196997/1‑100 (MQ=255)
                                       gATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtgg                                                             >  1:3793756/1‑100 (MQ=255)
                                         tggacgTGCCGGTTGTGAGTGGCATGCGAAGAACTCACCACCGCAGTATGCTGGTCCAAGTCGCTTACGTCAGGGGCTGGGTAAGTCGAAAAACg                                                                <  2:1829405/90‑1 (MQ=255)
                                         tGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtggtg                                                           >  1:3663326/1‑100 (MQ=255)
                                           cAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGAt                                                         >  1:1108556/1‑100 (MQ=255)
                                             aGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATgg                                                       <  1:1019339/100‑1 (MQ=255)
                                              gTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGt                                                      <  1:463684/100‑1 (MQ=255)
                                               tGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGtn                                                     <  2:3065970/100‑2 (MQ=255)
                                               tGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTa                                                     <  1:3094648/100‑1 (MQ=255)
                                                gCCGGTTCTGACTGGCAGCCGAAGAACTCACTACCGCAGCATGCTGGCCCAATTCGCTTAAGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGCTGGtgc                                                    >  2:2908722/1‑98 (MQ=255)
                                                 ccGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGACTGGGTCAGTCGAAAAACGTGGTGATGGTAcg                                                   >  1:2689296/1‑100 (MQ=255)
                                                  cGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTAcgc                                                  <  2:434861/100‑1 (MQ=255)
                                                   ggTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTAcgcg                                                 >  2:2457358/1‑100 (MQ=255)
                                                   ggTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTAcgcg                                                 >  1:2409754/1‑100 (MQ=255)
                                                    gTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACgcgc                                                <  1:3976333/100‑1 (MQ=255)
                                                    gTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACgcgc                                                <  1:3910234/100‑1 (MQ=255)
                                                       cTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAAt                                             >  2:736735/1‑100 (MQ=255)
                                                        tGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATg                                            <  2:225735/100‑1 (MQ=255)
                                                         gACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGc                                           >  1:2349138/1‑100 (MQ=255)
                                                             ggCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGc                                       >  2:1733645/1‑100 (MQ=255)
                                                             ggCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGc                                       <  1:1011364/100‑1 (MQ=255)
                                                               cAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGa                                     >  2:1630070/1‑100 (MQ=255)
                                                                 gCCGAAGAAGTCACCACCGCAGTAGGCCGGTCCAATACGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATg                                   <  2:1480117/100‑1 (MQ=255)
                                                                 gCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGacg                                   <  1:3835964/100‑3 (MQ=255)
                                                                 gCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATg                                   >  2:3405591/1‑100 (MQ=255)
                                                                   cGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATggg                                 <  1:2843583/100‑1 (MQ=255)
                                                                   cGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGATGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATggg                                 >  2:1167401/1‑100 (MQ=255)
                                                                     aagaaCTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGAGACAGACGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCg                               <  2:2809473/100‑1 (MQ=255)
                                                                       gaaCTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTc                             <  1:1537102/100‑1 (MQ=255)
                                                                        aaCTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCg                            >  2:1587267/1‑100 (MQ=255)
                                                                        aaCTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCg                            <  2:2381067/100‑1 (MQ=255)
                                                                        aaCTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCg                            >  1:853538/1‑100 (MQ=255)
                                                                         aCTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCg                            >  1:3816488/1‑99 (MQ=255)
                                                                         aCTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGa                           <  1:3034450/100‑1 (MQ=255)
                                                                         aCTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGa                           >  1:2537058/1‑100 (MQ=255)
                                                                          cTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGAc                          <  1:1237698/100‑1 (MQ=255)
                                                                          cTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGAc                          >  1:2417451/1‑100 (MQ=255)
                                                                          cTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGAc                          >  1:4122823/1‑100 (MQ=255)
                                                                          cTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGCGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGAc                          <  1:2577305/100‑1 (MQ=255)
                                                                            caccacCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTa                        >  2:6084/1‑100 (MQ=255)
                                                                            caccacCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTa                        >  2:69767/1‑100 (MQ=255)
                                                                            caccacCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGCAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTa                        >  1:1786044/1‑100 (MQ=255)
                                                                            caccacCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTa                        >  1:3268101/1‑100 (MQ=255)
                                                                            caccacCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTa                        >  2:1959299/1‑100 (MQ=255)
                                                                              ccaccGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACg                      >  2:320211/1‑100 (MQ=255)
                                                                                accGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCt                    >  2:3802330/1‑100 (MQ=255)
                                                                                 ccGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTg                   >  2:4069590/1‑100 (MQ=255)
                                                                                 ccGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTg                   >  2:907721/1‑100 (MQ=255)
                                                                                 ccGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTg                   >  1:4089214/1‑100 (MQ=255)
                                                                                  cGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGc                  <  2:2385972/100‑1 (MQ=255)
                                                                                   gCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCa                 <  2:1816058/100‑1 (MQ=255)
                                                                                   gCAGTATGCGGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCa                 <  2:1524698/100‑1 (MQ=255)
                                                                                   gCAGTAGGCTGGTCCAGTTCGCTGACGTCAGGGGCTGGGTCAGTCGAAAAACGGGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTg                   <  2:843352/98‑1 (MQ=255)
                                                                                     aGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCTCAATGCGTGCGATGGGCGTCTACTACGCTGCAGa               <  2:3945090/100‑1 (MQ=255)
                                                                                     aGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGa               >  2:2940814/1‑100 (MQ=255)
                                                                                      gTATGCTGGTCTAGTGCGCTTACGTCAGGGGCTGGGTCAGGCGAAAAACGTGGTGATGGTACGCGCAATCCGGGCGATCGGCGTCGACTACTCTGCAg                <  2:1988415/98‑1 (MQ=255)
                                                                                       taggccggTCCAGTTCGCTTACGTCGGGGGCTGAGGCAGGCGAAAAACGTGGTGATGGTACGCGCAATGCGAGCGATGGGCGTCGACTACGCTGCAGAat             <  2:3469110/94‑1 (MQ=255)
                                                                                        aTGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAata            >  1:3766380/1‑100 (MQ=255)
                                                                                        aTGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAata            <  1:3517580/100‑1 (MQ=255)
                                                                                         tGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAatat           >  1:990316/1‑100 (MQ=255)
                                                                                         tGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAatat           >  1:710214/1‑100 (MQ=255)
                                                                                            tGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTCCGCGCAATGCGTGCGATGGGCGTCGGCTACGCTGCAGAACATCTg        >  2:2481151/1‑100 (MQ=255)
                                                                                             ggTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGc       <  1:2650343/100‑1 (MQ=255)
                                                                                              ggccAATTGGCTTACGGCAGGGGCTGGCTCAGTCATGAAAAGTGGTGATGGTACGCGTAATGCGGGCGATGGGCGTGGACTACGCTGCAGAATATCTGCa      <  2:706171/98‑1 (MQ=255)
                                                                                                   |                                                                                                  
CTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGC  >  NC_000913/3524314‑3524511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: