Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,577,660 C→T 15.0% intergenic (‑68/+71) gntK ← / ← gntR gluconate kinase 2/d‑gluconate inducible gluconate regulon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,577,6600CT15.0% 577.9 / 73.6 237intergenic (‑68/+71)gntK/gntRgluconate kinase 2/d‑gluconate inducible gluconate regulon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (110/91);  new base T (16/20);  total (126/111)
Fisher's exact test for biased strand distribution p-value = 2.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.04e-01

Only 100 of 242 total aligned reads displayed.

TAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGA  >  NC_000913/3577561‑3577759
                                                                                                   |                                                                                                   
    tgtgGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGAc                                                                                                 <  1:3794135/100‑1 (MQ=255)
     gtggtgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACa                                                                                                <  1:1594273/100‑1 (MQ=255)
       ggtgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAat                                                                                              >  1:3411583/1‑100 (MQ=255)
       ggtgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAat                                                                                              >  2:614838/1‑100 (MQ=255)
        ntgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCGGCTGGACAatg                                                                                             >  1:3104151/2‑99 (MQ=255)
        gtgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAata                                                                                             <  1:2224669/100‑1 (MQ=255)
         tgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCGGCTGGACAatgt                                                                                            <  1:3282571/100‑3 (MQ=255)
         tgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCGGCTGGACAatgt                                                                                            <  1:787628/100‑3 (MQ=255)
         tgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAatat                                                                                            >  2:3651642/1‑100 (MQ=255)
         tgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAatat                                                                                            <  2:2002360/100‑1 (MQ=255)
         tgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAatat                                                                                            >  1:3845750/1‑100 (MQ=255)
           aTCATTGTTAGTCGTGCTCAAATTAGTCCCCCCACAATACAATAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAatat                                                                                            <  2:3973497/98‑1 (MQ=255)
           aTCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCGGCTGGACAatgtta                                                                                          <  1:2548421/100‑5 (MQ=255)
            tCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTCACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAg                                                                                         >  1:264122/1‑100 (MQ=255)
             cATGGTTAGTCGTTCTCAAAGTGGTGCCCCCCCAATACAAGATTTAAAAATGTTACGGGTCACTGTTATCGGTAACATTGTCCGGCTGGACAatgttacc                                                                                        <  2:403175/100‑7 (MQ=255)
             cATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTCTCGGTAACATTGTCCAGCCGGACAATATCAGa                                                                                        >  2:1963047/1‑100 (MQ=255)
             cATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCGGCTGGACAatgttacc                                                                                        <  2:413790/100‑7 (MQ=255)
             cATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCGGCTGGACAatgttacc                                                                                        <  1:98077/100‑7 (MQ=255)
              aTGGTTAGTCGTGCTCATAGTGGTGCCCCTACAATACAAGAATTAAAAATGTTATGGGTAACTGTTATCGGTAACATTGTCCGGCTGGACAatgttaccg                                                                                       <  2:809030/100‑8 (MQ=255)
               tGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAg                                                                                      >  2:4124520/1‑100 (MQ=255)
               tGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAg                                                                                      <  1:1125840/100‑1 (MQ=255)
               tGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAg                                                                                      <  1:2862658/100‑1 (MQ=255)
               tGGTTAGTCGTGCTCAAAGTGGTGCCCCCAAAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAg                                                                                      <  2:1783889/100‑1 (MQ=255)
                 gTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGc                                                                                     >  1:4055347/1‑99 (MQ=255)
                 gTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCa                                                                                    >  2:362310/1‑100 (MQ=255)
                  ttAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCaa                                                                                   <  1:109021/100‑1 (MQ=255)
                     gTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAatat                                                                                <  2:3762173/100‑1 (MQ=255)
                       cGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATatcc                                                                              >  1:3538221/1‑100 (MQ=255)
                       cGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATatc                                                                               >  1:3591069/1‑99 (MQ=255)
                        gTGCTCAAAGTGGTGCCCCAACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATatcca                                                                             <  2:1913261/100‑1 (MQ=255)
                         tGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATatccat                                                                            <  1:2629139/100‑1 (MQ=255)
                            tCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATAtccatcca                                                                         >  1:1184809/1‑100 (MQ=255)
                            tCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATAtccatcca                                                                         >  1:1289206/1‑100 (MQ=255)
                            tCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATAtccatcca                                                                         >  2:2322938/1‑100 (MQ=255)
                             cAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATccatccat                                                                        >  2:3385256/1‑100 (MQ=255)
                             cAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATccatccat                                                                        >  1:2614467/1‑100 (MQ=255)
                               aaGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGc                                                                      >  1:879565/1‑100 (MQ=255)
                                 gtggtgTCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCt                                                                    >  1:608524/1‑100 (MQ=255)
                                  tggtgCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCtt                                                                   >  1:2965476/1‑100 (MQ=255)
                                   ggtgCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTa                                                                  <  2:3467518/100‑1 (MQ=255)
                                   ggtgCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTa                                                                  <  2:1317505/100‑1 (MQ=255)
                                   ggtgCCCCCACAATACAAGAATTAAAAATGTTACGGGCAACCGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATCTCCATCCATGCCTTa                                                                  >  2:1970588/1‑100 (MQ=255)
                                     tgCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAg                                                                >  1:1800352/1‑100 (MQ=255)
                                      gCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGGTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCAGGCCATAAgt                                                               >  1:1864187/1‑100 (MQ=255)
                                       cccccACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAgtg                                                              <  1:1214677/100‑1 (MQ=255)
                                       cccccACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAgtg                                                              >  2:1603856/1‑100 (MQ=255)
                                        ccccACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAgtgt                                                             >  1:2446461/1‑100 (MQ=255)
                                        caccAAAATTCAATATTTAAATATTTTACTGTTAACTTTTATCGCTAACATTGTCCAGCCTGACAATATCATAAGCAATATCCATCCATGCCTTAAg                                                                <  2:3725410/95‑1 (MQ=255)
                                           cacaATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGta                                                            <  2:2090669/98‑1 (MQ=255)
                                             caATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAg                                                        <  1:55051/100‑1 (MQ=255)
                                             caATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAg                                                        <  1:1011534/100‑1 (MQ=255)
                                              aaTACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAgt                                                       >  2:3418980/1‑100 (MQ=255)
                                                  cAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGa                                                   <  1:1324576/100‑1 (MQ=255)
                                                  cAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGa                                                   >  2:2977321/1‑100 (MQ=255)
                                                   aaGAATTAAAGATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAgtgt                                                     >  2:19053/1‑97 (MQ=255)
                                                    aagaTTAAAAATTTTACGGGTAACTGTTATCGGGAAGATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGGGTATAAGTGTGAGc                                                 <  2:1206824/97‑1 (MQ=255)
                                                    aGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGc                                                 >  2:353082/1‑100 (MQ=255)
                                                       aTTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTa                                               <  2:2563322/99‑1 (MQ=255)
                                                       aTTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTAc                                              <  2:3224172/100‑1 (MQ=255)
                                                       aTTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTAc                                              <  2:1427692/100‑1 (MQ=255)
                                                        ttAAAAATGTTGCGGGTAACTGTTATCGGTAGCATTGTCTAGCCGGACAATATTAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTa                                               <  2:2100380/98‑1 (MQ=255)
                                                        ttAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCTGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACt                                             <  1:1986325/100‑1 (MQ=255)
                                                         tAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACtt                                            >  1:1899113/1‑100 (MQ=255)
                                                         tAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACtt                                            >  1:902505/1‑100 (MQ=255)
                                                         tAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACtt                                            >  2:3904352/1‑100 (MQ=255)
                                                          aaaaaTGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTc                                           <  1:2877767/100‑1 (MQ=255)
                                                           aaaaTGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCa                                          >  1:150385/1‑100 (MQ=255)
                                                              aTGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCACGCCTTAAGTGTATAAGTGCGAGCTACTTc                                           >  2:2601944/1‑96 (MQ=255)
                                                              aTGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATAAATCCAGGCCTTAAATGTATAAGTGTGAGCTACTTCAAAt                                       >  2:2456506/1‑100 (MQ=255)
                                                                 ttACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTtg                                    <  1:746669/100‑1 (MQ=255)
                                                                   aCGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTtgtg                                  <  2:2710163/100‑1 (MQ=255)
                                                                    cGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTgg                                 <  1:4060472/100‑1 (MQ=255)
                                                                    cGGGTAACTGTTATCGGTAACATTGTACAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAAttt                                     >  2:3704872/1‑96 (MQ=255)
                                                                       gTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCt                              >  1:3967901/1‑100 (MQ=255)
                                                                       gTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCt                              >  2:407317/1‑100 (MQ=255)
                                                                           cTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTaaa                          >  1:429857/1‑100 (MQ=255)
                                                                            tGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAAt                         <  2:1111421/100‑1 (MQ=255)
                                                                               tgcttctgataTTGTCCGGCTGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGa                      >  2:896439/11‑100 (MQ=38)
                                                                                gcttctgataTTGTCCGGCTGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGAt                     >  2:1085078/10‑100 (MQ=38)
                                                                                aTCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGAt                     >  1:3351852/1‑100 (MQ=255)
                                                                                 tCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGGGTATAAGTGTGAGCGACTTCAAATTTGTCGGCTTAAATAGa                      >  2:3855668/1‑98 (MQ=255)
                                                                                 tCGCTAACATTGTCCAGGCGGATAGTATGAGATGCAATATCCATCCAAGGCTTAAGTGTATATGTGTGAGCTACTTCAAATTTGTGGGCTGAAATAGATc                    <  2:3498097/100‑1 (MQ=255)
                                                                                  ttctgataTTGTCCGGCTGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTCGGCTTAAATAGATcc                   >  2:138843/8‑100 (MQ=255)
                                                                                  cGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGAt                     >  1:1663682/1‑98 (MQ=255)
                                                                                   tctgataTTGTCCGGCTGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCGACTTCAAATTTGTGGGCTTAAATAGTTCCg                  >  2:3067869/7‑100 (MQ=255)
                                                                                    gTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGc                 >  1:3454044/1‑100 (MQ=255)
                                                                                      gataTTGTCCGGCTGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGccc               >  1:305890/4‑100 (MQ=255)
                                                                                      gataTTGTCCGGCTGGACAATAGCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGATACTTCAAATTTGTGGGCTTAAATAGATcagac                >  2:1684467/4‑95 (MQ=255)
                                                                                      aaCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGccc               <  1:2097537/100‑1 (MQ=255)
                                                                                       aCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCg              <  2:316591/100‑1 (MQ=255)
                                                                                        naTTGTCCAGCCGGACAATATCAGAAGCAATATACACCCATGCCTTAAGTGAATAAGAGTGAGCTCCCTCAAATCTGTGGACTTAAATAGAGCCGCCAgg             >  2:664908/2‑100 (MQ=255)
                                                                                        cATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCgg             >  1:3699446/1‑100 (MQ=255)
                                                                                         aTTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGt            <  2:2327346/100‑1 (MQ=255)
                                                                                         aTTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGt            >  1:3664939/1‑100 (MQ=255)
                                                                                            gTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGaa         >  2:2210231/1‑99 (MQ=255)
                                                                                            gTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGAc         >  1:1316513/1‑100 (MQ=255)
                                                                                            gTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGAc         >  1:343007/1‑100 (MQ=255)
                                                                                                aGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAgg     >  1:2338003/1‑100 (MQ=255)
                                                                                                 gCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGt    <  2:2892771/100‑1 (MQ=255)
                                                                                                   cGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGa  >  1:169068/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGA  >  NC_000913/3577561‑3577759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: