Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 367,830 G→A 22.3% Y197Y (TAC→TAT)  mhpR ← mhp operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913367,8300GA22.3% 311.0 / 82.8 161Y197Y (TAC→TAT) mhpRmhp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (72/52);  new base A (21/15);  total (94/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

Only 100 of 164 total aligned reads displayed.

CACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATT  >  NC_000913/367731‑367929
                                                                                                   |                                                                                                   
  ccAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTa                                                                                                   >  1:2687725/1‑100 (MQ=255)
  ccAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATa                                                                                                   >  2:3127273/1‑100 (MQ=255)
     gATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAGcc                                                                                                <  2:2836728/100‑1 (MQ=255)
     gATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAAcc                                                                                                >  1:453731/1‑100 (MQ=255)
      aTTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAAccc                                                                                               <  1:3803660/100‑1 (MQ=255)
         cAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAAACctct                                                                                            >  2:734189/1‑100 (MQ=255)
          aGACAGCCAATCACCCGTTGTTCACTGCGCCGCGGTTCCGCGATCGATGCGATCTTCTCCCCCTGATCCCAGCCGCGGTAGTTCTGTCCTTACCCccct                                                                                            >  2:1101194/1‑99 (MQ=255)
          aGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGGCCATAACCctc                                                                                             >  2:2802816/1‑98 (MQ=255)
           gACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCTATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCttt                                                                                          >  2:155287/1‑100 (MQ=255)
           gACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCttt                                                                                          >  1:543613/1‑100 (MQ=255)
              aGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcg                                                                                       <  1:3479826/100‑1 (MQ=255)
              aGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcg                                                                                       <  2:3008651/100‑1 (MQ=255)
               gCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgc                                                                                      >  2:3511416/1‑100 (MQ=255)
                cgaaTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcg                                                                                     >  1:3830939/3‑100 (MQ=255)
                ccAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcg                                                                                     >  1:2007234/1‑100 (MQ=255)
                ccAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcg                                                                                     <  1:1372895/100‑1 (MQ=255)
                ccAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcg                                                                                     >  2:1404106/1‑100 (MQ=255)
                 cAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgc                                                                                    >  1:904034/1‑100 (MQ=255)
                  aaTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcg                                                                                   <  2:1231804/100‑1 (MQ=255)
                  aaTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcg                                                                                   >  1:3773335/1‑100 (MQ=255)
                    tCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgcgcg                                                                                 <  1:2803966/100‑1 (MQ=255)
                     cACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgcgc                                                                                >  1:1008530/1‑100 (MQ=255)
                      aCCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgcgcg                                                                               >  2:900311/1‑100 (MQ=255)
                      aCCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgcgcg                                                                               >  2:3049904/1‑100 (MQ=255)
                       cccGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTgcgcgcgcgcgc                                                                              >  1:1728865/1‑100 (MQ=255)
                        ccGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGcc                                                                             <  2:1853513/100‑1 (MQ=255)
                         cGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCa                                                                            >  2:1087737/1‑100 (MQ=255)
                          gttgttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAg                                                                           <  1:2622566/100‑1 (MQ=255)
                             gttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAAt                                                                        >  2:2827276/1‑100 (MQ=255)
                              ttCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATg                                                                       >  1:3022771/1‑100 (MQ=255)
                               tCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATgg                                                                      <  1:3057054/100‑1 (MQ=255)
                               tCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATgg                                                                      <  2:3668686/100‑1 (MQ=255)
                                cACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGc                                                                     <  2:927046/100‑1 (MQ=255)
                                 aCTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCt                                                                    >  2:2706088/1‑100 (MQ=255)
                                   tGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTc                                                                  >  1:1188100/1‑100 (MQ=255)
                                       cAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGc                                                              <  1:2951032/100‑1 (MQ=255)
                                       cAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGc                                                              <  1:4130914/100‑1 (MQ=255)
                                        aGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCt                                                             <  1:2827292/100‑1 (MQ=255)
                                        aGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCt                                                             <  1:3332734/100‑1 (MQ=255)
                                           ggTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTa                                                          <  2:3742571/100‑1 (MQ=255)
                                            gTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTaa                                                         >  2:2321752/1‑100 (MQ=255)
                                             tACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAAc                                                        <  1:3945919/100‑1 (MQ=255)
                                             tACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAAc                                                        <  1:1064201/100‑1 (MQ=255)
                                                 gCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAGTGGCTTCCCGCTTTAACGGtt                                                    >  2:817607/1‑100 (MQ=255)
                                                  cGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTc                                                   >  2:4020687/1‑100 (MQ=255)
                                                     tAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCg                                                <  1:638748/100‑1 (MQ=255)
                                                       gagGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGt                                               >  1:436941/1‑99 (MQ=255)
                                                        agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCTTGc                                             >  1:3614727/1‑100 (MQ=255)
                                                        agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCACCTTTAACGGTTCCCGTGc                                             >  2:2418974/1‑100 (MQ=255)
                                                        agGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGc                                             >  1:3569042/1‑100 (MQ=255)
                                                         ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                            >  2:3525532/1‑100 (MQ=255)
                                                         ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                            >  1:3172890/1‑100 (MQ=255)
                                                         ggCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGcc                                            <  2:2939043/100‑1 (MQ=255)
                                                            gATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGt                                         >  2:1912169/1‑100 (MQ=255)
                                                            gATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGt                                         >  1:1226348/1‑100 (MQ=255)
                                                            gATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGt                                         >  2:22450/1‑100 (MQ=255)
                                                            gATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGt                                         >  1:1402401/1‑100 (MQ=255)
                                                             aTCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGtt                                        <  2:2958348/100‑1 (MQ=255)
                                                              tcttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGtt                                        >  1:2468378/1‑99 (MQ=255)
                                                               cttcttCTCCTGCTCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg                                              <  2:3580154/92‑1 (MQ=255)
                                                               cttcgCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGa                                      <  2:1598052/100‑1 (MQ=255)
                                                                ttctCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGAt                                     <  1:4125317/100‑1 (MQ=255)
                                                                gtctcCTCCTGATCCCAGCCGCGGGAGTTCTTTCCATAACCCTCTTTGCGCGCGCGCGCCAGAGGGGCGTCCAGCTTTAACGTTTGCCGGGCCGGtgg                                       >  1:2251046/2‑96 (MQ=255)
                                                                 tctcCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATa                                    <  2:1650965/100‑1 (MQ=255)
                                                                 tctcCTCCTGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATa                                    >  2:3952268/1‑100 (MQ=255)
                                                                    cctcctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtc                                 >  2:1944901/1‑100 (MQ=255)
                                                                     ctcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGa                                      <  2:3858632/94‑1 (MQ=255)
                                                                      tcctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcat                               >  2:1161774/1‑100 (MQ=255)
                                                                       cctGATCCCAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGtcatc                              <  2:968784/100‑1 (MQ=255)
                                                                         tGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGCTAGTCATCAc                            >  2:1722688/1‑100 (MQ=255)
                                                                         tGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCAc                            >  1:36762/1‑100 (MQ=255)
                                                                          gATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCAcc                           >  1:1478479/1‑100 (MQ=255)
                                                                           aTCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCg                          <  1:1404115/100‑1 (MQ=255)
                                                                            ncccAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCgg                         >  1:1983300/2‑100 (MQ=255)
                                                                             cccAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCggg                        >  2:1258055/1‑100 (MQ=255)
                                                                              ccAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCgggg                       >  1:7620/1‑100 (MQ=255)
                                                                               cAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGc                      <  2:1352462/100‑1 (MQ=255)
                                                                               cAGCCGCGGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGc                      >  1:2242502/1‑100 (MQ=255)
                                                                                   cgcgGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGa                  <  2:3172871/100‑1 (MQ=255)
                                                                                    gcgGTAGTTCTGTCCGTCACCCTCTTTAAGCGCGCGCGCCAGAATGGCTTCGAGCTGTAACGATTCCCGTGCCAGTTGATAGTCATCATAGGGGCaggcg                 >  2:2571625/1‑98 (MQ=255)
                                                                                    gcgGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAg                 <  1:967437/100‑1 (MQ=255)
                                                                                    gcgGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAg                 <  1:852166/100‑1 (MQ=255)
                                                                                    gcgGTAGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAg                 <  2:1778941/100‑1 (MQ=255)
                                                                                     cgGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAgg                >  1:3779201/1‑100 (MQ=255)
                                                                                     cgGTAGTTCTGTCCATAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCt              >  2:1537073/1‑100 (MQ=255)
                                                                                      ggTAGTTCTGTCCGTACCCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTGCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGc               <  2:4025899/100‑1 (MQ=255)
                                                                                       gTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCt              <  2:1963907/100‑1 (MQ=255)
                                                                                        tAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTa             <  1:1679623/100‑1 (MQ=255)
                                                                                         aGTTCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTaa            >  1:2863026/1‑100 (MQ=255)
                                                                                          gTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAAc           >  1:639835/1‑100 (MQ=255)
                                                                                          gTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAAc           >  2:3467083/1‑100 (MQ=255)
                                                                                           ttCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACa          <  2:1138388/100‑1 (MQ=255)
                                                                                            tCTGTCCATAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACAt         >  1:3826687/1‑100 (MQ=255)
                                                                                            tCTGGCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACAt         <  2:3767502/100‑1 (MQ=255)
                                                                                             cTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCACCACCGGGGCGGGAGGCTAACAtt        >  2:92094/1‑100 (MQ=255)
                                                                                              tGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGAAGGCTAACa          >  2:3573024/1‑97 (MQ=255)
                                                                                                tCCGTAACCCTCTTTGCGCGCGCGCGCCATAATGGCTTCCAGCTTTAACGGTTCCCGTGCCATTTGATAGTCATCTTCTTGGCGGGAGGCTAACATTTCg     <  2:599360/100‑1 (MQ=255)
                                                                                                tCCGTAACCCTCTTGGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTTCCGTGCCAGTTGATAGTCATCACCGTGGCGGGAGGCTAACATTTCg     <  2:231512/100‑1 (MQ=255)
                                                                                                  cGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGAt   <  2:3879317/100‑1 (MQ=255)
                                                                                                   gTAACCCTCTTTGCGCGCGCGCCCCAGGATGGCTTCCAGCTTTAACGGTTCCAGTGCTAGTTGATAGCCATCACCGGGGCGGGAGGCTAACATTTCGAtt  <  1:1550772/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATT  >  NC_000913/367731‑367929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑