Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,638,525 A→G 47.2% E295G (GAA→GGA)  pitA → phosphate transporter, low‑affinity; tellurite importer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,638,5250AG47.2% 20.4 / inf 247E295G (GAA→GGA) pitAphosphate transporter, low‑affinity; tellurite importer
Reads supporting (aligned to +/- strand):  ref base A (73/56);  new base G (60/55);  total (134/113)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

Only 100 of 250 total aligned reads displayed.

TACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAG  >  NC_000913/3638427‑3638624
                                                                                                  |                                                                                                   
tacccgtGATGCCATCAACAACGTCCAAGCTTACTTTGAGCCGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCGGATCAGTTAGTACCGGCTCCGGaa                                                                                                    >  2:530975/1‑100 (MQ=255)
  cccgtGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGc                                                                                                  <  1:3329116/100‑1 (MQ=255)
  cccgtGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGc                                                                                                  <  2:404296/100‑1 (MQ=255)
   ccgtGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCt                                                                                                 <  2:3285268/100‑1 (MQ=255)
      tGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGgc                                                                                              <  1:2390759/100‑1 (MQ=255)
       gATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGgcg                                                                                             <  1:1520341/100‑1 (MQ=255)
           ccATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGcgcaac                                                                                         >  1:187816/1‑100 (MQ=255)
            cATCAACAACGTCGAAGCTTACTTTGCGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGcgcaacg                                                                                        <  1:859028/100‑1 (MQ=255)
            cATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGcgcaacg                                                                                        >  1:2641289/1‑100 (MQ=255)
            cATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGcgcaacg                                                                                        >  2:3509920/1‑100 (MQ=255)
               caacaaCGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGcgcaacgcta                                                                                     >  2:2508624/1‑98 (MQ=255)
                aacaacGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCgcaacgcaac                                                                                    <  2:1961743/100‑1 (MQ=255)
                 acaacGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCgcaacgcaac                                                                                    >  2:837821/1‑99 (MQ=255)
                  caacGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCt                                                                                  <  2:1853679/100‑1 (MQ=255)
                  caacGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCt                                                                                  >  1:2295171/1‑100 (MQ=255)
                    acGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGc                                                                                <  2:334903/100‑1 (MQ=255)
                     cGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCg                                                                               <  1:3031235/100‑1 (MQ=255)
                      gTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCgg                                                                              <  1:2275209/100‑1 (MQ=255)
                       tCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGa                                                                             >  1:3191501/1‑100 (MQ=255)
                       tCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAAACTGCGGa                                                                             >  1:2092692/1‑100 (MQ=255)
                           aGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCGACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTc                                                                         <  2:612506/100‑1 (MQ=255)
                               tACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACt                                                                     <  1:3826196/100‑1 (MQ=255)
                               tACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGACCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCAc                                                                      >  2:1016786/1‑99 (MQ=255)
                                aCTTTGAGCTGCATCCAGCGCTGGACGATCAGGCTACCGGTGCTGTGCAGTAAGTACCGCCGACGGACGCTGGCGCAACGGAACGTTCGGAGcaccac                                                                      >  2:301226/1‑98 (MQ=255)
                                aCTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTg                                                                    <  2:1870775/100‑1 (MQ=255)
                                 cTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGc                                                                   <  1:1044238/100‑1 (MQ=255)
                                 cTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGc                                                                   <  1:3233912/100‑1 (MQ=255)
                                 cTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGc                                                                   <  1:1080549/100‑1 (MQ=255)
                                  tttGCGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGc                                                                   <  2:2461759/99‑1 (MQ=255)
                                  tttGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGcc                                                                  <  2:873823/100‑1 (MQ=255)
                                   ttGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCGGGCGCAACGCAACCTGCGGAGTTCCACTGCCa                                                                 <  2:1934390/100‑1 (MQ=255)
                                       gcagcaTCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCg                                                             >  2:2840112/1‑100 (MQ=255)
                                       gcagcaTCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCg                                                             >  2:1724658/1‑100 (MQ=255)
                                        cagcaTCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGt                                                            <  1:2610808/100‑1 (MQ=255)
                                         agcaTCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTc                                                           >  1:2061714/1‑100 (MQ=255)
                                          gcaTCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCg                                                          >  2:34489/1‑100 (MQ=255)
                                              ccTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATa                                                      <  2:1433718/100‑1 (MQ=255)
                                               cTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATAc                                                     <  1:2638756/100‑1 (MQ=255)
                                               cTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATAc                                                     >  2:2976786/1‑100 (MQ=255)
                                                 gcgcTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCa                                                   <  2:3876129/100‑1 (MQ=255)
                                                   gctgctCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCAtt                                                 >  2:3111890/1‑100 (MQ=255)
                                                   gctgctCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCAt                                                  <  2:1229707/99‑1 (MQ=255)
                                                   gctgctCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCAtt                                                 >  1:2452194/1‑100 (MQ=255)
                                                      gctCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAAc                                              >  1:1970083/1‑100 (MQ=255)
                                                       ctCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAAcg                                             >  2:1524382/1‑100 (MQ=255)
                                                       ctCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAAcg                                             <  1:1666236/100‑1 (MQ=255)
                                                       ctCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCACCGGAAGCTGGCGCAACGCTACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAAcg                                             <  2:2257864/100‑1 (MQ=255)
                                                         cAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAAcgcg                                           >  2:1667737/1‑100 (MQ=255)
                                                         cAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAAcgcg                                           >  1:687507/1‑100 (MQ=255)
                                                          tctcAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACgcgc                                          <  2:3062248/97‑1 (MQ=255)
                                                           aaCAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCt                                         >  1:1199860/1‑100 (MQ=255)
                                                             cAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACgcgcaga                                       >  1:3060990/1‑97 (MQ=255)
                                                                ctcttccgatcTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAAcc                                    <  2:260205/90‑1 (MQ=255)
                                                                 cTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCg                                   <  1:2974091/100‑1 (MQ=255)
                                                                  tACCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGAGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCg                                   <  2:1148171/99‑1 (MQ=255)
                                                                  tACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGc                                  >  1:3672226/1‑100 (MQ=255)
                                                                   aCCGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGcc                                 <  1:4087328/100‑1 (MQ=255)
                                                                   aCCGGTGCTAATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGACATCCGTCGAATACCATTAACGCGCTCAACCGcc                                 <  2:4113928/100‑1 (MQ=255)
                                                                    ccGGTGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCt                                <  1:2880529/100‑1 (MQ=255)
                                                                    ccGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCt                                <  2:445021/100‑1 (MQ=255)
                                                                    ccGGTGCGGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGcc                                 <  1:2770774/99‑1 (MQ=255)
                                                                      ggcgCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACCGCCAGCCGTCGACTACCACTAACGCCCCCAACCGCCTGa                              >  2:3924677/4‑100 (MQ=255)
                                                                        tGCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGaaa                            >  1:113544/1‑100 (MQ=255)
                                                                         gCTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAg                           >  1:896728/1‑100 (MQ=255)
                                                                          cTGATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAgg                          <  2:2312755/100‑1 (MQ=255)
                                                                          cTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAgg                          <  1:3419550/100‑1 (MQ=255)
                                                                            gATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTa                        >  1:972732/1‑100 (MQ=255)
                                                                            gATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTa                        <  1:3488516/100‑1 (MQ=255)
                                                                            gATCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTa                        >  1:76397/1‑100 (MQ=255)
                                                                            gATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTa                        <  1:4064019/100‑1 (MQ=255)
                                                                            gATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTa                        >  1:1440369/1‑100 (MQ=255)
                                                                             aTCAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTAt                       >  1:2223442/1‑100 (MQ=255)
                                                                              tCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATg                      <  1:1568872/100‑1 (MQ=255)
                                                                               cAGTTAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGt                     <  1:612554/100‑1 (MQ=255)
                                                                               cAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGt                     <  1:1301508/100‑1 (MQ=255)
                                                                                 gTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATCCCATTAAGACGCTCAACCGCCCGACAGGTATGt                     >  2:3751894/1‑98 (MQ=255)
                                                                                 gTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTg                   >  1:3959614/1‑100 (MQ=255)
                                                                                  ttAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGa                  <  2:567862/100‑1 (MQ=255)
                                                                                  ttAGTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGa                  <  2:3786488/100‑1 (MQ=255)
                                                                                  ttAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGa                  >  1:1241714/1‑100 (MQ=255)
                                                                                   tAGTACTGGATCCGGAAGCGGGCGCGACGGTAACTGCGGAGTTCCACTAGCATTCGTCGAATGCCTTGAACGCGTTTAATCGACTGGTAGGGATGTTg                   <  2:2699726/98‑1 (MQ=255)
                                                                                    aGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGacc                <  2:3584422/100‑1 (MQ=255)
                                                                                     gTACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGacca               <  2:3566688/100‑1 (MQ=255)
                                                                                      tACCGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGaccac              >  1:486235/1‑100 (MQ=255)
                                                                                       aCCGGCTCCGGGAGCTGGCGCAACGGAACCTGCGGAGCTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGCAAGGTATGTTGaccac              >  1:191001/1‑99 (MQ=255)
                                                                                       aCCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGCGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGaccacc             >  2:2882775/1‑100 (MQ=255)
                                                                                       aCCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGaccacc             <  1:3133224/100‑1 (MQ=255)
                                                                                       aCCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGaccacc             >  2:2061629/1‑100 (MQ=255)
                                                                                        ccGGCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCg            >  1:1751545/1‑100 (MQ=255)
                                                                                        ccGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCg            >  1:3752295/1‑100 (MQ=255)
                                                                                           gCTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGAtg         >  1:220613/1‑100 (MQ=255)
                                                                                            cTCCGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCCCAACCGCCTGAAAGGTATGTTGACCACCGAtg         >  2:2198536/1‑99 (MQ=255)
                                                                                            cTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGAtgt        >  2:3956474/1‑100 (MQ=255)
                                                                                               cGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGTGGa     >  1:1605436/1‑100 (MQ=255)
                                                                                               cGGGAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGTGGa     <  1:3944784/100‑1 (MQ=255)
                                                                                                ggAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGTGGaa    <  1:2576362/100‑1 (MQ=255)
                                                                                                ggAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGTGGaa    <  1:3858275/100‑1 (MQ=255)
                                                                                                ggAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGACATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGTGGaa    >  1:2912821/1‑100 (MQ=255)
                                                                                                 gAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGTGGaaa   >  1:208441/1‑100 (MQ=255)
                                                                                                 gAAGCTGGCGCAACGCAACCTGCGGAGTGCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCGGAAAGGTATGTTGACCACCGATGTGGaaa   <  2:3851557/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
TACCCGTGATGCCATCAACAACGTCGAAGCTTACTTTGAGCAGCATCCTGCGCTGCTCAAACAGGCTACCGGTGCTGATCAGTTAGTACCGGCTCCGGAAGCTGGCGCAACGCAACCTGCGGAGTTCCACTGCCATCCGTCGAATACCATTAACGCGCTCAACCGCCTGAAAGGTATGTTGACCACCGATGTGGAAAG  >  NC_000913/3638427‑3638624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: