Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,669,920 A→G 22.5% E110G (GAA→GGA)  treF → cytoplasmic trehalase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,669,9200AG22.5% 354.7 / 104.6 194E110G (GAA→GGA) treFcytoplasmic trehalase
Reads supporting (aligned to +/- strand):  ref base A (72/78);  new base G (17/27);  total (89/105)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

Only 100 of 204 total aligned reads displayed.

CCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTAAC  >  NC_000913/3669821‑3670019
                                                                                                   |                                                                                                   
 cTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGaa                                                                                                    <  2:1823862/100‑1 (MQ=255)
 cTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGaa                                                                                                    >  1:1704478/1‑100 (MQ=255)
  tttCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGaaa                                                                                                   >  2:3331722/1‑100 (MQ=255)
   ttCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGaata                                                                                                  <  1:2808509/100‑3 (MQ=255)
    tCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAAc                                                                                                 <  1:3703472/100‑1 (MQ=255)
    tCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAAc                                                                                                 <  1:2189530/100‑1 (MQ=255)
     cccGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAAcc                                                                                                <  1:1487237/100‑1 (MQ=255)
     cccGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAAcc                                                                                                <  1:2170545/100‑1 (MQ=255)
     cccGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAAcc                                                                                                <  1:3400513/100‑1 (MQ=255)
            gcgcACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTg                                                                                         <  1:138793/100‑1 (MQ=255)
             cgcACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTgg                                                                                        >  1:1436569/1‑100 (MQ=255)
               cACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCt                                                                                      >  1:2533126/1‑100 (MQ=255)
               cACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCt                                                                                      <  2:879483/100‑1 (MQ=255)
                aCCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTg                                                                                     <  1:3295863/100‑1 (MQ=255)
                  cTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGcc                                                                                   >  1:2698559/1‑100 (MQ=255)
                  cTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGcc                                                                                   >  2:1448403/1‑100 (MQ=255)
                    aaaaTGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCgg                                                                                 >  1:3556743/1‑100 (MQ=255)
                    aaaaTGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCgg                                                                                 <  2:3349290/100‑1 (MQ=255)
                    aaaaTGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCgg                                                                                 >  1:1655846/1‑100 (MQ=255)
                     aaaTGGACCCGTTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAATCCACTTCTGGCTGCCgg                                                                                 <  2:2372688/99‑1 (MQ=255)
                     aaaTGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCACGTTTGTTGAAAACCACTTCTGGCTGCCg                                                                                  >  2:1025886/1‑98 (MQ=255)
                       aTGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAgg                                                                              <  1:1544503/100‑1 (MQ=255)
                       aTGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAgg                                                                              <  1:2646860/100‑1 (MQ=255)
                        tGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGt                                                                             >  2:2955201/1‑100 (MQ=255)
                        tGGACCCGCTGGATAGCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTAGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAgg                                                                              >  2:2056533/1‑99 (MQ=255)
                          gACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCt                                                                           >  1:394497/1‑100 (MQ=255)
                            cccGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGGCTTGGGCAAGTTTGTTGAAAACCACTTCTGGCTGCGTGAGGTCTAc                                                                         >  2:3296246/1‑100 (MQ=255)
                            cccGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTAc                                                                         >  1:1976087/1‑100 (MQ=255)
                            cccGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTAc                                                                         <  1:3743403/100‑1 (MQ=255)
                             ccGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACt                                                                        >  1:200976/1‑100 (MQ=255)
                             ccGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACt                                                                        >  1:3986376/1‑100 (MQ=255)
                                   gATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCg                                                                  <  1:1347606/100‑1 (MQ=255)
                                   gATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCg                                                                  <  2:1375145/100‑1 (MQ=255)
                                     ttacTTAGTCTGCTGCCGTTAATTGGGCCGGCATCGTGATTTTGACTTCCGCCAGTTTGTTGGAAGCCACTTCTGGCTGCCGGCGGTCTACTc                                                                       <  2:484726/90‑1 (MQ=255)
                                     tatCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAg                                                                >  2:375231/1‑100 (MQ=255)
                                     tatCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAg                                                                >  1:1861350/1‑100 (MQ=255)
                                     tatCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAg                                                                >  2:638329/1‑100 (MQ=255)
                                      atCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgt                                                               <  1:1083809/100‑1 (MQ=255)
                                      atCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgt                                                               <  2:4029715/100‑1 (MQ=255)
                                      atCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgt                                                               <  1:2355654/100‑1 (MQ=255)
                                      atCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgt                                                               <  1:2095908/100‑1 (MQ=255)
                                       tCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgta                                                              <  2:75028/100‑1 (MQ=255)
                                       tCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgta                                                              >  1:3083204/1‑100 (MQ=255)
                                        cTTAATCCGCTACCGTAAAGTGCGCCTTCATCGTCATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtat                                                             >  2:367983/1‑100 (MQ=255)
                                        cTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtat                                                             >  1:1206778/1‑100 (MQ=255)
                                         ttAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatg                                                            <  1:388676/100‑1 (MQ=255)
                                         ttAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatg                                                            <  2:3116934/100‑1 (MQ=255)
                                           aaTCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACTACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatgta                                                          <  2:2545574/100‑1 (MQ=255)
                                            aTCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatgtat                                                         >  2:1775151/1‑100 (MQ=255)
                                            aTCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatgtat                                                         <  1:1580831/100‑1 (MQ=255)
                                             tCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATc                                                        >  2:4112668/1‑100 (MQ=255)
                                              ccGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCg                                                       >  1:774702/1‑100 (MQ=255)
                                               cGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCgg                                                      <  1:3446903/100‑1 (MQ=255)
                                               cGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCgg                                                      <  1:564075/100‑1 (MQ=255)
                                                gCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGa                                                     <  1:2955218/100‑1 (MQ=255)
                                                 cTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGAc                                                    >  2:2471542/1‑100 (MQ=255)
                                                 cTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGAc                                                    <  2:3985942/100‑1 (MQ=255)
                                                    ccGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCg                                                 >  1:99704/1‑100 (MQ=255)
                                                    ccGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCg                                                 <  1:1173056/100‑1 (MQ=255)
                                                    ccGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCg                                                 >  1:3555122/1‑100 (MQ=255)
                                                            tGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTc                                         <  2:684049/100‑1 (MQ=255)
                                                              cgcCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTccc                                       <  1:3977996/100‑1 (MQ=255)
                                                              cgcCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTccc                                       <  1:213643/100‑1 (MQ=255)
                                                                ccGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTg                                     >  1:474584/1‑100 (MQ=255)
                                                                ccGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTg                                     <  2:2732196/100‑1 (MQ=255)
                                                                  gTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGaa                                   <  2:3827665/100‑1 (MQ=255)
                                                                     aTCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAaga                                >  2:3526194/1‑100 (MQ=255)
                                                                       cGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGc                              <  1:3036244/100‑1 (MQ=255)
                                                                        gTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCa                             <  2:3081156/100‑1 (MQ=255)
                                                                            ttttGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATc                         <  1:2470844/100‑1 (MQ=255)
                                                                             tttGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCg                        >  1:259780/1‑100 (MQ=255)
                                                                             tttGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAACGAGCATATCg                        >  1:3864189/1‑100 (MQ=255)
                                                                             tttGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCg                        >  2:2669315/1‑100 (MQ=255)
                                                                                gACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGAcc                     <  1:2589457/100‑1 (MQ=255)
                                                                                gACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGAcc                     <  2:3378209/100‑1 (MQ=255)
                                                                                 aCTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCa                    >  1:579437/1‑100 (MQ=255)
                                                                                 aCTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCa                    <  1:1831356/100‑1 (MQ=255)
                                                                                 aCTTGCGAAAGTTTCTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGAca                     <  2:819463/99‑2 (MQ=255)
                                                                                  cTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAg                   >  2:2913727/1‑100 (MQ=255)
                                                                                   ttGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTGCTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGc                  <  2:2156184/100‑1 (MQ=255)
                                                                                   ttGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGc                  <  1:2422830/100‑1 (MQ=255)
                                                                                    tGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAg                   >  2:189910/1‑98 (MQ=255)
                                                                                     gcgcAAGTTTGTTGAAAACCACTTCTGGCTGCCGGGGGGCTCCTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACGAGCtg                >  2:1552073/1‑100 (MQ=255)
                                                                                     gcgcAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCtg                >  1:1260911/1‑100 (MQ=255)
                                                                                     gcgcAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCtg                >  2:4010409/1‑100 (MQ=255)
                                                                                       gcAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCtgtg              <  1:547049/100‑1 (MQ=255)
                                                                                         aaGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGc            >  1:3589989/1‑100 (MQ=255)
                                                                                          aGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGcc           >  1:794423/1‑100 (MQ=255)
                                                                                            tttGTTGAAAACCACTTCTTGCTGCCCGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCgg         <  2:3053840/100‑1 (MQ=255)
                                                                                             ttgttgAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGt        <  1:821121/100‑1 (MQ=255)
                                                                                               gttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGc      >  1:1133615/1‑100 (MQ=255)
                                                                                               gttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGc      <  1:290495/100‑1 (MQ=255)
                                                                                               gttgAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGc      <  2:1698519/100‑1 (MQ=255)
                                                                                               gttgAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGc      <  2:1092643/100‑1 (MQ=255)
                                                                                                ttgAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCt     >  1:227716/1‑100 (MQ=255)
                                                                                                ntgAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCt     >  1:228159/2‑100 (MQ=255)
                                                                                                 tgAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCTAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTa    >  2:2205524/1‑100 (MQ=255)
                                                                                                 tgAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTa    >  1:4024160/1‑100 (MQ=255)
                                                                                                 tgAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTa    >  1:3160278/1‑100 (MQ=255)
                                                                                                  gAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTaa   >  2:380654/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTAAC  >  NC_000913/3669821‑3670019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑